• The Journal of the Korea Contents Association
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• v.12 no.12
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• pp.313-320
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• 2012

A dental treatment room is always exposed to diverse kinds of pathogenic bacteria, and may be a mediating place of cross-infection given being contaminated the interior of a room through several routes in the form of patient's secretion and aerosol. The main agent of preventing cross-infection is a dental hygienist in the dental treatment and the dental treatment room where are scattered about a risk of cross-infection. A dental hygienist needs to have right recognition on infection control before being active as a clinical expert. This infection-control recognition level is influenced from the clinical practice. Accordingly, to survey recognition of infection control, a self-administered questionnaire research was conducted targeting 314 students who are fixed the clinical practice as regular subject in the junior course out of curriculum for the Department of Dental Hygiene at some of 4-year universities. Data collection was performed from December 9, 2011 to February 22, 2012. Except 11 copies of questionnaire with insincere response among the collected materials, 303 copies were finally analyzed by using SPSS WIN 20.0. The following conclusions were obtained. In the infection disease section, both on and off campus showed 4.89 points from 'the importance of recognizing the infections prevention', 4.65 points from 'recognizing the compulsory preventative injection for hepatitis type B', 4.77 points from 'recognizing the necessity of the preventative injection for hepatitis type B', 4.71 points from 'whether practice the prevention in reality or not', and 4.76 points from 'the educational helps to the prevention'. In other words, the section recorded the highest and meaningful points. It is considered to be needed the development in systematic and diverse infection-control educational programs and the differentiated education depending on school year for dental hygiene students.

• Korean journal of applied entomology
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• v.30 no.3
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• pp.212-218
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• 1991

Pathogenicity of Spodoptera exigua nuclear polyhedrosis virus (SeMNPV) against the host insect and 8 species of lepidopterous insects and cross infection of baculoviruses to third instar of S. exigua larvae were studied to determine as a biocontrol agent for S. exigua. The median lethal concentrations($LC_{50}$)of the SeMNPV to egg mass was $2.855\times10^5$ PIBs/ml and higher than that to the larvae of S. exigua. Mortality of the SeMNPV in third ins tar larvae was more increased than that in first and fifth instar of S. exigua larvae by 1.16 and 4.11 times, respectively. The median lethal times($LT_{50}$) to $1.56\times10^6$ PIBs/ml was in the range of 4.25 to 5.04 days. Infectivity of the SeMNPV against eight species of lepidopterous insects was showed only in the host insect, S. exigua. Autographa cali/ornica MNPV, Mamestra barassicae MNPV, and Trichoplusia ni MNPV were cross-infected to third instar of S. exigua larvae among ten of baculoviruses tested.

• Parasites, Hosts and Diseases
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• v.25 no.2
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• pp.188-194
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• 1987

The development of antibody titers and crossreaction between Sarcocystis and Toxoplasma were investigated by means of IF A test and ELISA in pigs experimentally infected with $1.5{\times}10^6$ S. suicanis sporocysts and 10,000 T. gondii oocysts, respectively. The intact and soluble Sarcocystis antigens were prepared from the bradyzoites harvested by peptic digestion of infected pork. The intact and soluble Toxoplasma antigens were prepared from the tachyzoites in mouse peritoneal cavity. IgG antibodies in pigs infected with Sarcocystis and Toxoplasma, respectively were detected first at 2 weeks post infection on both IF A test and ELISA. The antibody titer to Toxoplasma reached its maximum at 6 weeks post infection and decreased thereafter. The antibody titer to Sarcocystis reached its maximum terminally. The cross-reaction titer in pigs infected with Toxoplasma against Sarcocystis antigen was up to 1 : 16 in IFA test and up to 1 : 32 in ELISA. The titer in control group was below 1 : 4 in both reactions.

• Journal of Digital Convergence
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• v.18 no.11
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• pp.329-336
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• 2020

The study investigated the perception and experience of infection control targeting dental users. During July-August 2020, 198 adults over the age of 20 were surveyed on general characteristics, infection control awareness and experience, and improvement. Analysis was performed using PASW Statistics ver 18.0. The research results, 91% of dental users recognized that infection control was important. In the recognition of infection control were highly investigated oral treatment equipment sterilization, hand hygiene and glove replacement before and after treatment by dental staff. And dental users was relatively low the replacement of disposable gowns and safety glasses for each patient by medical staff. The dental staff are doing well in personal protection and instrument sterilization. Surface disinfection and water quality management needed improvement. It was meaningful to suggest improvement in infection control based on the perception and experience from the perspective of dental users. It is expected to be used as basic data necessary for high-quality medical services through infection control in dental medical institutions.

• Parasites, Hosts and Diseases
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• v.30 no.4
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• pp.259-262
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• 1992

This study was performed to investigate experimental transmission of Cryptosporidium parvum in a calf. A 25-day-old Korean native calf was inoculated per os with $1{\times}10^6$ C. parvum oocysts isolated from a Korean mouse. The calf commenced oocyst discharge in feces on post-inoculation day 4, and continued until the aah 11. The number of discharged oocysts Peaked($4.9{\times}10^5$) on post-inoculation day 6. However, the calf did not show signs of diarrhea. The present results indicate that C. parvum is cross-transmissible between the calf and the mouse.

• Journal of Oral Medicine and Pain
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• v.34 no.3
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• pp.237-245
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• 2009

The purpose of this study was to investigate the clinical efficacy of latex cover developed for dental handpiece on contamination of microorganisms during dental treatment and to determine whether it can be an alternative to conventional sterilization such as autoclaving. E. fecaelis was used as a experimental microorganism instead of oral flora. Experimental bowl with 2 cm of rectangular cavity was fabricated for handpiece operating instead of oral cavity. Latex covers ($Orokeeper^{(R)}$, Orobiotech Co., Korea) and several handpieces were used after sterilization by autoclave. Four experiments were performed to evaluate bacterial contamination related with (1) various parts of dental handpiece, (2) swabbing time with alcohol sponge, (3) postoperative air-water spraying time and (4) consecutive use of latex covers without autoclaving. The results show that face of handpiece uncovered with latex cover was severely contaminated than the covered area and that most bacteria were removed by swabbing face and head area of dental hand-piece and by air-water spraying more than 15 seconds nearly up to the level of sterilization. Conclusively it can be suggested that use of latex cover for handpiece during dental procedure, swabbing with alcohol sponge is air-water spraying for more than 15 seconds after use of dental handpiece should be very useful and practical for prevention of cross infection and should be an alternative method for the sterilization of dental handpiece under some difficult situations not being able to sterilize a handpiece with autoclave.

• Korean Journal of Microbiology
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• v.49 no.1
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• pp.51-57
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• 2013

As facilities and equipments for learning activities in college campuses are handled by mass public, their contact surfaces may function as major routes of cross-infection of microbial pathogens. However, unlike items in cafeteria which is the typical target for campus hygiene, those surfaces are not under regular surveillance or sanitary maintenance. In this study, I made a quantitative comparison of the risk of being exposed to microbial pathogens from use of learning facilities such as classrooms and library to the risk from use of cafeteria, for about 1,500 students in a college. Regarding total coliforms as surrogate model of bacterial pathogens, exposure rates were estimated for each item in learning facilities and cafeterias by devising deterministic exposure algorithms based on bacterial abundance, contract rates and transfer rates. The exposure rate in cafeterias was 1.0 CFU/day while learning facilities imposed the rate of 0.5 CFU/day, which reaches a half of the exposure rate in cafeterias. However, 70% of students were exposed more in learning facilities than cafeteria because individuals had different frequencies in using cafeteria. Based on the results, some human-contact surfaces of learning facilities, including elevator buttons, may require regular sanitary maintenance. An efficient sanitary maintenance considering seasonality in diversity of pathogens involved with cross-infections is suggested besides improvement of personal hygiene among students.

• Journal of Sericultural and Entomological Science
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• v.34 no.2
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• pp.26-31
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• 1992

Flacherie virus (FV) and Densonucleosis virus (DNV) of the silkworm, Bombyx mori, which give the most severest damage to the silk production in korea, were fed on the mulberry wild silkworm, Bombyx mori mandarina, the mulberry pyralid, Gryphodes phyloalis, and the American fall webworm, Hypantria cunea, to investigate cross infectivity by serological and histopathological at observation. By the Ouchterlony's double difusion test the mulberry wild silkworm was infected with both FV and DNV type 1 (DNV-1) and the mulberry pyralid with DNV-1, so those were confirmed the cross infection. But the American fall webworm was not recognized the cross infection by the same method. The infection and multiplication of the FV in the mulberry wild silkworm was observed in the cytoplasm of the goblet cell with the appearance of the virus-specific vesicle. In DNV-1 infection to the mulberry wild silkworm and the mulberry pyralid, the nuclei of columnar cell in the midgut of both insects was hypertrophied and the nuclei of midgut cell of the mulberry pyralid positively stained with the feulgen stain. Multiplication of DNV-1 in the midgut cell of the mulberry wild silkworm was replicated in two different patterens as linear arrays and large masses, while that of DNV-1 in the muberry pyralid was multiplied as virus masses in several portion of the nuclei of the midgut cell.

• Parasites, Hosts and Diseases
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• v.22 no.2
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• pp.223-228
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• 1984

Seven cases of surgically proven sparganosis were serologically tested by means of microELISA for their specific IgG antibody levels. For that purpose, crude saline extract of spargana from snake, Natrix tigrina lateralis was prepared and used as antigen. The sparganosis sera were also tested with Paragonimus and Cysticercus antigens to observe the cross reactivity. A total of 71 sera from normal control, ectopic and pulmonary paragonimiasis, clonorchiasis, cysticerCOSIS and Taenia saginata cases were also included. Except for one case of old calcified infection, all of 6 human sparganosis showed higher serum levels of specific IgG antibody when the differential point of positive reaction was set at the absorbance value of 0.25 (the sensitivity being 85.7%). In control and other helminthic infections, all except 3 cases of T. saginata infection showed negative reaction to sparganum antigen (the specificity being 90.7%). None of sparganosis cases showed cress reactivity to Paragonimus and Cysticercus antigens. Undiluted cerebrospinal l1uid also showed high levels of antibody when central nervous system was invaded. The serologic diagnosis by means of micro ELISA could be a useful tool in epidemiological study of human sparganosis in susceptible population, as well as in individual diagnosis.

• The Journal of the Korean dental association
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• v.31 no.12 s.295
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• pp.897-904
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• 1993