• Title/Summary/Keyword: 광합성 실험

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Effect of Submergence and Air Exposure of the Shoot on Growth, Nutrient Uptake and Photosynthesis in Monochoria vaginalis Presl. (물달개비 경엽(莖葉)의 침수여부(沈水與否)에 따른 생장(生長), 양분흡수(養分吸收) 및 광합성(光合成) 비교(比較))

  • Soh, C.H.;Yang, K.S.;Kwon, Y.W.
    • Korean Journal of Weed Science
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    • v.16 no.1
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    • pp.14-20
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    • 1996
  • Growth, nutrient uptake and photosynthesis as affected by submersion of shoot in pickerel weed (Monochoria vaginalis Presl.) were determined. The shoots of pickerel weeds in hydroponic culture were subjected to the submerged or emerged condition at 3- or 5-leaf stage for 8 or 10 days. Under submerged condition, growth in plant height was enhanced, but leaf number, leaf area, fresh and dry weight were reduced compared to those under the emerged condition. Similar responses in growth to submergence were obtained with the pickerel weeds rooted in the soil. Under submergence, chlorophyll content increased during the first 2 days, but thereafter remarkably decreased at 3-leaf stage and after the first 4 days at 5-leaf stage. Compared to the emerged condition, uptakes of $NH_4\;^+$-N, $NO_3\;^-$-N, $P_2O_5$ and $K^+$ were reduced, but uptakes of $Ca^{++}$ and $Mg^{++}$ increased under the submerged condition. Photosynthetic rate of shoot under water, measured by $CO_2$electrode, showed the maximum by 210 ${\mu}$moles $HCO_3\;^-$/g F.W. at the 8th day after submergence(DAS) at 3-leaf stage and 320 ${\mu}$moles $HCO_3\;^-$/g F. W. at 6 DAS at 5-leaf stage. These results indicate that pickerel weeds grow much better when the shoot is air-exposed and are less tolerable to submergence at 3 leaf-stage than at 5-leaf stage.

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Applicability of a Photosynthetic Ciliate, Mesodinium rubrum MR-MAL01 -Usefulness as a Live Prey Species for the Marine Aquaculture of Bivalves- (광합성 섬모류 Mesodinium rubrum MR-MAL01의 응용성(1) 이매패류 종묘생산을 위한 먹이생물 유용성)

  • 김형섭;명금옥;조수근;이원호
    • Journal of Aquaculture
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    • v.17 no.2
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    • pp.115-121
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    • 2004
  • In a series of feeding experiments using the newly established the first laboratory strain of a temperate photosynthetic ciliate, Mesodinium rubrum MR-MAL01, direct evidence was obtained for ingestion of the cultured M. rubrum cells by the bivalves like the Korean scallop, Chlamys farreri and Manila clam. Ruditapes phil-ippinarum. Each experimental Korean scallop and Manila clam removed up to 9,590 and 23,200 cells min-1, respectively. Small particles almost identical to the ruptured cells of MR-MAL01 culture were observed in the gut of the experimental bivalves, although no intact M. rubrum cell was found. In a 28 days rearing experiment, Isoshrysis galbana (KMCC H-2), a microalga, supported better growth of small Manila clam spat (0.46 mm in shell length) than MR-MAL01 strain. For the large Manila clam spat (0.84 mm in shell length), however. MR-MAL01 strain was a better prey item. Mass culture methods for this photosynthetic marine ciliate may be developed for use as live feed in aquaculture of the spat and broodstock of bivalve and small-mouthed fish larvae.

Synergistic Effects of Low Dose Gamma Irradiation and Growth Regulators on Seed Germination, Growth and Photosynthesis in Rice (Oryza sativa L.) (벼의 종자 발아와 생육 및 광합성에 대한 저선량 감마선과 생장조절물질의 상승작용 효과)

  • Baek Myung-Hwa;Chung Byung Yeoup;Kim Jin-Hong;Wi Seung Gon;Kim Jae-Sung;Lee In-Jung
    • Korean Journal of Environmental Biology
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    • v.23 no.1
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    • pp.64-70
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    • 2005
  • To investigate the synergistic effects of low dose gamma irradiation and growth regulators on the growth and photosynthesis in rice (Oryza sativa L.), laboratory and greenhouse experiments were conducted using 4-year-old rice seeds. In the laboratory experiment, the germination rate was increased in 0.001 ppm IBA treatment, showing the synergistic effect of gamma irradiation and growth regulators. The seedling growth was increased by treatment of GA₃ and IBA, the irradiated groups having higher than the non-irradiated ones. Particularly, it was remarkable in 0.001 ppm IBA. In greenhouse experiment, seedling growth was increased in response to a combination of gamma irradiation and 0.001 ppm IBA. Effective quantum yield of PSⅡ(Ф/sub PSⅡ/) and photochemical quenching (qP) were increased, while non-photochemical quenching (qN) was decreased by 0.001 ppm IBA. A synergistic effect of gamma irradiation and IBA was only found in seedling growth. The present results suggest that low dose gamma irradiation and growth regulator could synergistically stimulate seedling growth.

Characteristics of Photosynthesis and Dry Matter Accumulation in Japonica and Tongil type Rice (수도자포니카 및 통일형 품종의 광합성 및 물질생산 특성)

  • 허훈;양덕조;류경열
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.1
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    • pp.45-53
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    • 1992
  • This studies were conducted to investigate the physiological characteristics of photosynthesis and dry matter accmulation of 2 Tongil type and 2 Japonica type varieties under different temperature and light intensity condition. Photosynthetic activities were lower in Tongil type varieties than Japonica type at low temperature(17$^{\circ}C$), but higher in Tongil type varieties at high temperature in each growth stages. The degradation rate of photosynthesis was higher in Tongil type varieties than Japonica type varieties at low temperature and Tongil type varieties were showed high photosynthetic activities at high temperature ($25^{\circ}C$). Specific Leaf Area in each growth stages were the highest at tillering and increased from panicle formation stage to heading stage. The ratio of respiration to photosynthesis (R /P$\times$200) into upper three leaves were significantly high in third leaf and showed same slope in each varieties. CGR, NAR were higher in Tongil type varieties than Japonica type varieties and yields, havesting index were showed high in Tongil type varieties.

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Effect of Overhead Flooding Stress on Photosynthesis and Growth in Rice (벼의 관수기간 및 수질이 광합성과 생육에 미치는 영향)

  • 이상각;강병화
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.3
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    • pp.209-214
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    • 2001
  • Physiological responses of rice to the flooding time of different water turbidity (clear water, sub-muddy water, muddy water) were analyzed as photosynthesis, chlorophyll fluorescence, transpiration, and physiological recovery. Photosynthetic rate was higher as turbidity increased and decreased as flooding time was extended. Floodings of 36 hrs and 42 hrs were resulted in 25% and 50% decrease of photosynthetic rate, respectively. Transpiration rate was higher in high turbidity (increased 30%, 25%, and 20% in clear, sub-muddy, and muddy water, respectively) and in increased floodings. Fv/Fm decreased as increased turbidity and flooding time. About 20% decrease of Fv/Fm was recorded in 48 hrs and 36 hrs after flooding with clear water and sub-muddy water(including muddy water), respectively. Total nitrogen was decreased with flooding treatment. Significant decrease of total nitrogen was occurred 36 hrs after flooding with muddy water, Dry weight measured 2 weeks after flooding treatment as an indication of recovery of flooding stress didn't show significant difference with turbidity, but significantly decreased as flooding time was prolonged. About 25% and 50% decreases were found in 24 hrs and 42 hrs flooding time, respectively. furthermore, 48 hrs of flooding with sub-muddy and muddy water resulted in no physiological recovery. Photosynthetic rate was decreased 15% and 10% with clear water and muddy water(including sub-muddy water), respectively. The rate was dramatically decreased 42 hrs after flooding. Transpiration rate increased about 20% regardless of turbidity and flooding time. We found transitory decrease of photosynthetic and transpiration rate at the point of 24 hrs after flooding and right after do-flooding.

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The Effect of Seedlings Density in Nursery on Biomass Production and Growth Characteristic of Styrax obassia (생육밀도가 쪽동백나무 유묘의 물질생산량과 생장특성에 미치는 영향)

  • Choi, Jeong-Ho;Yoo, Se-Kuel;Lee, Soo-Won;Oh, Seok-Gui
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.217-221
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    • 2007
  • To provide the basic data essential for developing varieties of broad-leaved tree, the study investigated the utmost growth requirement for the growth characteristic and biomass production in accordance with planting density of Styrax obassia. The planting density of seedling can be a significant factor in contributing growth of seedling for tree growing in general. While growth of tree according to $1m^2$ planting density of Styrax obassia showed an excellent growth in tree height and the root collar diameter from $49no./m^2$, those low planting density showed decreased tendency with tree height growth while increasing the root collar diameter growth. As similar to the growth characteristic, the biomass production showed significant and statistic difference in $49no./m^2$, showing high volume of biomass production which is $3.12{\pm}0.80g$ compare to other processing section and high figure with T/R ratio which is 1.59. The special trait of photosynthetic also showed relatively high photosynthetic rate in $49no./m^2\;and\;64no./m^2$ of Styrax obassia and as the density increase, photosynthetic efficiency decreased. The plant showed stable and physiological planting pattern, displaying the best photosynthetic rate, which was the final metabolism through reserving proper space in the growth and development environment condition. This obstacle of required space essential for growth substantially deteriorated planting and ultimately, it demonstrated lower tendency of photosynthetic rate, which is the highest level of metabolism.

Role of Mesophyll Morphology in Determination of Leaf Photosynthesis in Field Grown Soybeans (포장생육대두의 엽광합성과정에서 엽육세포 형태의 역할)

  • Yun, Jin Il;Lauer, Michael J.;Taylo, S.Elwynn
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.6
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    • pp.560-567
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    • 1991
  • Photosynthetic variation in field grown soybean [Glycine max (L.) Merr. cv Hodgson78] was studied in relation to leaf anatomical variation. Variations in mesophyll morphology were accentuated by manipulating source and sink size. At R3 stage, two treatments were started: one was thinning and continu-ous debranching(6. 5 plants rather than 26 plants per m of row and remaining plants were debranched weekly), and the other was continuous partial depodding (allowing only one pod to develop at each mainstem node). Gas exchange characteristics, mesophyll cell volume and surface area per unit leaf surface, and microclimatic parameters were measured on the intact terminal leaflet at the 10th node. Observations were made 5 times with 3 to 4 day intervals starting R4 stage. Two models were used to compute leaf photosynthetic rates: one considered no effect of mesophyll morphology on photosynthesis, and the other considered potential effects of variations in mesophyll cell volume and surface area on diffusion and biochemical processes. Seventy nine percent of total photosynthetic variations observed in the experiment was explained by the latter, while 69% of the same variations was explained by the former model. By incorporating the mesophyll morphology concept, the predictability was improved by 14.6% in the field condition. Additional Index Words: photosynthesis model, leaf anatomy, Glycine max (L.) Merr., mesophyll surface area, mesophyll cell volume.

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Development of a Chinese cabbage model using Microsoft Excel/VBA (엑셀/VBA를 이용한 배추 모형 제작)

  • Moon, Kyung Hwan;Song, Eun Young;Wi, Seung Hwan;Oh, Sooja
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.2
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    • pp.228-232
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    • 2018
  • Process-based crop models have been used to assess the impact of climate change on crop production. These models are implemented in procedural or object oriented computer programming languages including FORTRAN, C++, Delphi, Java, which have a stiff learning curve. The requirement for a high level of computer programming is one of barriers for efforts to develop and improve crop models based on biophysical process. In this study, we attempted to develop a Chinese cabbage model using Microsoft Excel with Visual Basic for Application (VBA), which would be easy enough for most agricultural scientists to develop a simple model for crop growth simulation. Results from Soil-Plant-Atmosphere-Research (SPAR) experiments under six temperature conditions were used to determine parameters of the Chinese cabbage model. During a plant growing season in SPAR chambers, numbers of leaves, leaf areas, growth rate of plants were measured six times. Leaf photosynthesis was also measured using LI-6400 Potable Photosynthesis System. Farquhar, von Caemmerer, and Berry (FvCB) model was used to simulate a leaf-level photosynthesis process. A sun/shade model was used to scale up to canopy-level photosynthesis. An Excel add-in, which is a small VBA program to assist crop modeling, was used to implement a Chinese cabbage model under the environment of Excel organizing all of equations into a single set of crop model. The model was able to simulate hourly changes in photosynthesis, growth rate, and other physiological variables using meteorological input data. Estimates and measurements of dry weight obtained from six SPAR chambers were linearly related ($R^2=0.985$). This result indicated that the Excel/VBA can be widely used for many crop scientists to develop crop models.

Effects of Leaf and Pod Removal on Photosynthesis and Assimilate Partition in Soybean (적엽ㆍ제협처리가 콩의 광합성과 동화물질 배분에 미치는 영향)

  • Woong Tae, Kim;Rak Chun, Seong;Harry C, Minor
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.2
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    • pp.159-165
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    • 1993
  • To clarify the effects of sink demand for assimilate on leaf photosynthetic rate, tissue composition, and leaf senescence of soybean [Glycine max (L.) Merr.] J plants, pod and leaf tissues were removed at growth stage $R^3$. Plant responses were measured every 10days from 2 through 42days following treatment. Leaves of depodded plants exhibited increased starch and chlorophyll contents and specific leaf weight. Stomatal resistance was also increased and leaf photosynthetic rate was reduced. Dry weight of vegetative tissues except leaves was increased by pod removal. Leaf removal resulted in a decreased starch content of leaves from 22 to 42days after treatment and that of roots at all sampling times. Specific leaf weight was decreased while leaf photosynthetic rate was increased. Stomatal resistance and chlorophyll content were little affected. Weight per seed was decreased 3.0% by leaf removal. Except for the seed, tissue protein contents were increased by pod removal but decreased by leaf removal, however, seed protein content was not affected by either. Apparent senescence was delayed by depodding. Both apparent and functional senescence were accelerated by leaf removal.

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Physiological Response and Growth Performance of Parasenecio firmus under Different Shading Treatments (차광처리에 따른 병풍쌈의 생리반응 및 생장특성)

  • Lee, Kyeong-Cheol;Lee, Hak-Bong;Park, Wan-Geun;Han, Sang-Sup
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.2
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    • pp.79-89
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    • 2012
  • This study was conducted to investigate the chlorophyll contents, photosynthetic characteristics, chlorophyll fluorescence, and growth performance of Parasenecio firmus under changing light environment. Parasenecio firmus was grown under non-treated (full sunlight) and three different shading conditions (88~93%, 65~75% and 45%~55% of full sunlight) for the experiment. Total chlorophyll content, photochemical efficiency (Fv/Fm), T/R ratio, specific leaf area (SLA), leaf area ratio (LAR), and leaf weight ratio (LWR) were increased with increasing shading level, but decreased dark respiration. Therefore, light absorption and light utilization efficiency were improved under the low intensity light. Plants under 65~75% of full sunlight had best maximum photosynthetic rate and net apparent quantum yield in May. On the other hand, the non-treated plants had lower maximum photosynthetic rate, photochemical efficiency, and chlorophyll content than the treated ones. Parasenecio firmus considered to be a sciophyte, is fairly sensitive to high intensity light. If 88-93% of full sunlight lasts for a long period, photosynthetic capacity will be sharply decreased, though limiting light. These results suggest that growth of Parasenecio firmus adapted to 65~75% of full sunlight.