• Clinical and Experimental Pediatrics
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• v.45 no.7
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• pp.862-874
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• 2002

Purpose : We tried to evaluate whether the detection rate of Helicobacter pylori in gastric biopsy specimens could be improved by using pre-embedding immunoelectron microscopy. Methods : A total of 119 children who complained of upper gastrointestinal symptoms were endoscoped at the Gyeongsang National University Hospital from July, 1996 to July, 1999. Five biopsy specimens(three for urease test, one for hematoxylin-eosin(H & E) staining, and one for preembedding immunoelectron microscopy) were obtained from each antrum and body. Immunoblotting analysis were also performed. Results : Among the 119 patients, H. pylori were found in 116 patients(97.5%) by the immunoelectron microscopy. Among three patients who were found H. pylori negative in immunoelectron microscopy, two patients showed H. pylori in H & E stained slides and one patient was urease test positive(color change within six hours). Urease tests were positive in 107 patients(89.9 %). The positive rate of immunoblotting tests was 81.5%. However, only 13 patients(10.9%) showed H. pylori on the H & E stained antrum or body tissue. Conclusion : In this study, we found H. pylori histopathologically in most of the pediatric patients who complained of upper gastrointestinal symptoms. This study showed that pre-embedding immunoelectron microscopic examinations can be used as a gold standard in the diagnosis of childhood H. pylori infection. However, this method also has limited capacity to detect widely scattered H. pylori compared to the other histopathologic diagnostic methods.

• Clinical and Experimental Pediatrics
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• v.46 no.2
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• pp.128-136
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• 2003

Purpose : Hyper IgM syndrome(HIGM) is characterized by severe recurrent bacterial infections with decreased serum levels of IgG, IgA, and IgE but elevated IgM levels. Recently, it has been classified into three groups; HIGM1, HIGM2 and a rare form of HIGM. HIGM1 is a X-linked form of HIGM and has now been identified as a T-cell deficiency in which mutations occur in the gene that encodes the CD40 ligand molecule. HIGM2 is an autosomal recessive form of HIGM. Molecular studies have shown that the mutation of HIGM2 is in the gene that encodes activation-induced cytidine deaminase(AID). Recently, another rare form of X-linked HIGM syndrome associated with hypohydrotic ectodermal dysplasia has been identified. We encountered a patient with a varient form of HIGM2. To clarify the cause of this form of HIGM, we evaluated the peripheral B cells of this patient. Methods : The lymphocytes of the patient were prepared from peripheral blood. B cells were immortalized with the infection of EBV. Cell cycle analysis was done with the immortalized B cells of the patient. Peripheral mononuclear cells were stained with monoclonal anti-CD40L antibody. Total RNA was extracted from the peripheral mononuclear cells. After RT-PCR, direct sequencing for CD40L gene and HuAID gene were done. Immunostainings of a lymph node for CD3, CD23, CD40, Fas-L, bcl-2, BAX were done. Results : The peripheral B cells of this patient showed normal expression of CD40L molecule and normal sequencing of CD40L gene, and also normal sequencing of AID gene. Interestingly, the peripheral B cells of this patient showed a decreased population of G2/mitosis phase in cell cycles which recovered to normal with the stimulation of IL-4. Conclusion : We suspect that the cause of increased serum IgM in this patient may be from a decrease of G2/mitosis phase of the peripheral B cells, which may be from the decreased production or secretion of IL-4. Therefore, this may be a new form of HIGM.

• Journal of the Korean earth science society
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• v.27 no.4
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• pp.450-463
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• 2006

Seasonal threshold values for fog detection over the ten airport areas within the Korean Peninsula have been derived from the data of polar-orbit Aqua/Terra MODIS and geostationary GOES-9 during a two years. The values are obtained from reflectance at $0.65{\mu}m\;(R_{0.65})$ and the difference in brightness temperature between $3.7{\mu}m\;and\;11{\mu}m\;(T_{3.7-11})$. In order to examine the discrepancy between the threshold values of two kinds of satellites, the following four parameters have been analyzed under the condition of daytime/nighttime and fog/clear-sky, utilizing their simultaneous observations over the Seoul metropolitan area: brightness temperature at $3.7{\mu}m$, the temperature at $11{\mu}m,\;the\;T_{3.7-11}$ for day and night, and the $R_{0.65}$ for daytime. The parameters show significant correlations (r<0.5) in spatial distribution between the two kinds of satellites. The discrepancy between their infrared thresholds is mainly due to the disagreement in their spatial resolutions and spectral bands, particularly at $3.7{\mu}m$. Fog detection from GOES-9 over the nine airport areas except the Cheongju airport has revealed accuracy of 60% in the daytime and 70% in the nighttime, based on statistical verification. The accuracy decreases in foggy cases with twilight, precipitation, short persistence, or the higher cloud above fog. The sensitivity of radiance and reflectance with wavelength has been analyzed in numerical experiments with respect to various meteorological conditions to investigate optical characteristics of the three channels.

• Childhood Kidney Diseases
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• v.2 no.1
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• pp.50-59
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• 1998

Purpose : Hepatitis B virus (HBV) infection has been involved in several forms of immune-related glomerulopathy but the pathogenic role of HBV infection is not clear. To evaluate the clinicopathological features of HBV-associated glomerulopathy, a clinicopathological analysis and immunohistochemical stain for HBs Ag and HBe Ag were done. Methods : Clinicopathological features of HBV-associated glomerulopathy were analyzed with renal biopsies in 28 HBsAg seropositive patients from April 1990 to February 1997 at Pusan Paik Hospital, and immunohistochemical evaluation for HBsAg and HBeAg was done in renal tissues. Light microscopic, immunofluorescent and electron microscopic examination and immunohistochemical staining for HBsAg (DAKO) and HBeAg (BIONIKE) of renal tissue were performed. Result ; 1. The age distribution was 6 to 73 years old, and eight were children and 20 were adults. Male : female ratio was 3:1. Nineteen (67.9%) and 21 (75.0%) of 28 cases showed hematuria and proteinuria, respectively at the time of biopsy. Sixteen (57.2%) of them had nephrotic syndrome. 2. Liver function test was performed in 11 patients and seven (63.6%) of them showed increased AST and ALT levels. Liver biopsy was done in three patients and revealed findings of chronic active hepatitis. 3. HBV-associated glomerulopathy was membranous glomerulonephritis (MGN) in 10 (35.7%), mesangiopathy in 8 (28.6%), membranoproliferative glomerulonephritis (MPGN) in 7 (25.0%) and minimal change disease in 3 (10.7%) out of 28 cases. 4. Ultrastructurally HBV-associated MGN showed conspicuous subepithelial deposits with intramembranous, mesangial and subendothelial deposits and proliferation of mesangial cells and matrix, which were suggestive of MPGN. In HBV-associated MPGN, intramembranous and subepithelial deposits were scattered. 5. Immunohistochemical staining revealed no expression for HBsAg, but positive reaction for HBeAg along capillary wall in 8 cases (28.6%), of which 3 cases were checked for serum HBeAg, all showed positivity. Conclusion : HBV-associated glomerulopathy showed a wide morphologic spectrum and overlapping ultrastructural features in MGN and MPGN, and the activity of hepatitis B virus may be related to the development of HBV-associated glomerulopathy but further studies are recommended to confirm this relationship.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.21 no.3
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• pp.99-104
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• 2011

A stoichiometric mixture of evaporating materials for $MgGa_2Se_4$ single crystal thin films was prepared from horizontal electric furnace. The crystal structure of these compounds has a rhombohedral structure with lattice constants $a_0=3.953\;{\AA}$, $c_0=38.890\;{\AA}$. To obtain the single crystal thin films, $MgGa_2Se_4$ mixed crystal was deposited on thoroughly etched semi-insulating GaAs(100) substrate by the Hot Wall Epitaxy (HWE) system. The source and substrate temperatures were $610^{\circ}C$ and $400^{\circ}C$, respectively. The crystalline structure of the single crystal thin films was investigated by the double crystal X-ray rocking curve and X-ray diffraction ${\omega}-2{\theta}$ scans. The carrier density and mobility of $MgGa_2Se_4$ single crystal thin films measured from Hall effect by van der Pauw method were $6.21{\times}10^{18}\;cm^{-3}$ and 248 $cm^2/v{\cdot}s$ at 293 K, respectively. The optical absorption of $MgGa_2Se_4$ single crystal thin films was investigated in the temperature range from 10 K to 293 K. The temperature dependence of the optical energy gap of the $MgGa_2Se_4$ obtained from the absorption spectra was well described by the Varshni's equation, $E_g(T)=E_g(0)-({\alpha}T^2/T+{\beta})$. The constants of Varshni's equation had the values of $E_g(0)=2.34\;eV$, ${\alpha}=8.81{\times}10^{-4}\;eV/K$ and ${\beta}=251\;K$, respectively.

• Journal of the Korean earth science society
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• v.33 no.2
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• pp.139-147
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• 2012

Mongolia's solar-meteorological resources map has been developed using satellite data and reanalysis data. Solar radiation was calculated using solar radiation model, in which the input data were satellite data from SRTM, TERA, AQUA, AURA and MTSAT-1R satellites and the reanalysis data from NCEP/NCAR. The calculated results are validated by the DSWRF (Downward Short-Wave Radiation Flux) from NCEP/NCAR reanalysis. Mongolia is composed of mountainous region in the western area and desert or semi-arid region in middle and southern parts of the country. South-central area comprises inside the continent with a clear day and less rainfall, and irradiation is higher than other regions on the same latitude. The western mountain region is reached a lot of solar energy due to high elevation but the area is covered with snow (high albedo) throughout the year. The snow cover is a cause of false detection from the cloud detection algorithm of satellite data. Eventually clearness index and solar radiation are underestimated. And southern region has high total precipitable water and aerosol optical depth, but high solar radiation reaches the surface as it is located on the relatively lower latitude. When calculated solar radiation is validated by DSWRF from NCEP/NCAR reanalysis, monthly mean solar radiation is 547.59 MJ which is approximately 2.89 MJ higher than DSWRF. The correlation coefficient between calculation and reanalysis data is 0.99 and the RMSE (Root Mean Square Error) is 6.17 MJ. It turned out to be highest correlation (r=0.94) in October, and lowest correlation (r=0.62) in March considering the error of cloud detection with melting and yellow sand.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.3
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• pp.229-235
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• 2001

The thermal and photometric properties of mulching materials modify the radiation and energy balance on the mulched soil surface and thereby change the soil temperature. The soil surface energy balances and soil temperatures under the mulching treatments of non-mulched control, recycled paper (RPM), and black polyethylene film (BPFM) were compared before and after the establishment of potato canopy. On August 30 in 1998 when potato was not emerged yet and solar radiation was 17.9 MJ $m^{-2}$${day}^{-1}$ , the net radiation of the soil surface was estimated as 10.(1, 2. 4, and 1.3 MJ $m^{-2}$${day}^{-1}$ under the control, BPFM, and RPM, respectively. The sensible and latent heat loss from the soil surface was 9.65 MJ $m^{-2}$${day}^{-1}$ in the control, most of the net radiation being lost through evaporation and convection, whereas it amounted only to 1.39 MJ $m^{-2}$${day}^{-1}$ in BPFM and 1.36 MJ $m^{-2}$${day}^{-1}$ in RPM. Therefore, the soil heat fluxes were 0.36 1.02, and 0.06 MJ m$^{-2}$ day$^{-1}$ under the control, BPFM and RPM, respectively. On September 27 when potato canopy was fully developed, the soil surface net radiation in the control was sharply decreased as compared to that of Aug. 30, whereas the net radiation of the mulched soil surfaces showed little changes. The soil heat flux was -0.01, 0.95, and 0.12 MJ $m^{-2}$${day}^{-1}$ at the soil surface under the control, BPFM and RPM, respectively. As the mulching treatments brought about such alteration of energy partitioning into the soil, the highest soil temperature was recorded in BPFM and the lowest in RMP without regard to potato canopy development. However, the soil temperature differences among the treatments become smaller when potato canopy were fully developed.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.1
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• pp.48-57
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• 2015

In a sodium-cooled fast reactor, which is a Generation-IV reactor, refueling is conducted by rotating, but not opening, the reactor head to prevent a reaction between the sodium, water and air. Therefore, an inspection technique that checks for the presence of any obstacles between the reactor core and the upper internal structure, which could disturb the rotation of the reactor head, is essential prior to the refueling of a sodium-cooled fast reactor. To this end, an ultrasound-based inspection technique should be employed because the opacity of the sodium prevents conventional optical inspection techniques from being applied to the monitoring of obstacles. In this study, a ranging inspection technique using a plate-type ultrasonic waveguide sensor was developed to monitor the presence of any obstacles between the reactor core and the upper internal structure in the opaque sodium. Because the waveguide sensor installs an ultrasonic transducer in a relatively cold region and transmits the ultrasonic waves into the hot radioactive liquid sodium through a long waveguide, it offers better reliability and is less susceptible to thermal or radiation damage. A 10 m horizontal beam waveguide sensor capable of radiating an ultrasonic wave horizontally was developed, and beam profile measurements and basic experiments were carried out to investigate the characteristics of the developed sensor. The beam width and propagation distance of the ultrasonic wave radiated from the sensor were assessed based on the experimental results. Finally, a feasibility test using cylindrical targets (corresponding to the shape of possible obstacles) was also conducted to evaluate the applicability of the developed ranging inspection technique to actual applications.

• Korean Journal of Weed Science
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• v.8 no.2
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• pp.187-198
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• 1988

The second leaves from 30 days old seedlings of two rice cultivars which were selected as tolerant (cv. Chokoto) and susceptible (cv. Weld pally) cultivar were soaked in the concentration of $10^{-6}$, $10^{-5}$, $10^{-4}$ and $10^{-3}M$ of oxyfluorfen for 10, 15 and 22hrs and anatomical characteristics were abserved. Dipping to the solutions were carried out either directly to the attached leaves or to the seperated leaves. Development of any symptoms in epidermis, bundle sheath, mesophyll cells and bulliform cells were microscopically inspected. Both cultivars showed reductionin leaf thickness, but the susceptible ones was more sensitive than the tolerant. The degradation and disappearance of epidermal cell layer, breakage of bundle sheath cells, shrinkage of mesophyll cells and disappearance of bulliform cells were general response as affected by oxyfluorfen treatment. The susceptible cultivars showed such responses at the concentration of $10^{-5}M$ for 10 hrs while tolerant ones $10^{-3}M$, for 10 hrs. Those treatments were more effective in seperated leaves than in attached ones. The epicuticular wax layer of leaves treated as above for 20 hrs was inspected by SEM. Weld pally, the susceptible cultivar (Weld pally) showed rapid cleavage of wax layer under $10^{-5}M$ concentration while the tolerant (Chokoto) showed only minor damage on wax layer at the concentration of $10^{-3}M$.

• Journal of the Korean earth science society
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• v.31 no.6
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• pp.600-607
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• 2010

In 2008, multiple episodes of large-scale transport of natural airborne particles and anthropogenically affected particles from different sources in the East Asian continent were identified in the National Oceanic and Atmospheric Administration (NOAA) satellite RGB-composite images and the mass concentrations of ground level particulate matters. To analyze the aerosol size distribution during the large-scale transport of atmospheric aerosols, both aerosol optical depth (AOD; proportional to the aerosol total loading in the vertical column) and fine aerosol weighting (FW; fractional contribution of fine aerosol to the total AOD) of Moderate resolution Imaging Spectroradiometer (MODIS) aerosol products were used over the East Asian region. The six episodes of massive natural airborne particles were observed at Cheongwon, originating from sandstorms in northern China, Mongolia and the loess plateau of China. The $PM_{10}$ and $PM_{2.5}$ stood at 70% and 16% of the total mass concentration of TSP, respectively. However, the mass concentration of $PM_{2.5}$ among TSP increased as high as 23% in the episode in which they were flowing in by way f the industrial area in east China. In the other five episodes of anthropogenically affected particles that flowed into the Korean Peninsula from east China, the mass concentrations of $PM_{10}$ and $PM_{2.5}$ among TSP reached 82% and 65%, respectively. The average AOD for the large-scale transport of anthropogenically affected particle episodes in the East Asian region was measured at $0.42{\pm}0.17$ compared with AOD ($0.36{\pm}0.13$) for the natural airborne particle episodes. Particularly, the regions covering east China, the Yellow Sea, the Korean Peninsula, and the east Korean sea were characterized by high levels of AOD. The average FW values observed during the event of anthropogenically affected aerosols ($0.63{\pm}0.16$) were moderately higher than those of natural airborne particles ($0.52{\pm}0.13$). This observation suggests that anthropogenically affected particles contribute greatly to the atmospheric aerosols in East Asia.