• Title/Summary/Keyword: 관로 전달 특성

Search Result 176, Processing Time 0.024 seconds

CELL CULTURE STUDIES OF MAREK'S DISEASE ETIOLOGICAL AGENT (조직배양(組織培養)에 의한 Marek 병(病) 병원체(病原體)의 연구(硏究))

  • Kim, Uh-Ho
    • Korean Journal of Veterinary Research
    • /
    • v.9 no.1
    • /
    • pp.23-62
    • /
    • 1969
  • Throughout the studies the following experimental results were obtained and are summarized: 1. Multiplication of agents in primary cell cultures of both GF classical and CR-64 acute strain of Marek's disease infected chicken kidneys was accompanied by the formation of distinct transformed cell foci. This characteristic nature of cell transformation was passaged regularly by addition of dispersed cell from infected cultures to normal chicken kidney cell cultures, and also transferred was the nature of cell transformation to normal chick-embryo liver and neuroglial cell cultures. No cytopathic changes were noticed in inoculated chick-embryo fibroblast cultures. 2. The same cytopathic effects were noticed in normal kidney cell monolayers after the inoculation of whole blood and huffy coat cells derived from both forms of Marek's disease infected chickens. In these cases, however, the number of transformed cell foci appearing was far less than that of uninoculated monolayers prepared directly from the kidneys of Marek's disease infected chickens. 3. The change in cell culture IS regarded as a specific cell transformation focus induced by an oncogenic virus rather than it plaque in slowly progressing cytopathic effect by non-oncogenic viruses, and it is quite similar to RSV focus in chick-embryo fibroblasts in many respects. 4. The infective agent (cell transformable) were extremely cell-associated and could not be separated in an infective state from cells under the experimental conditions. 5. The focus assay of these agents was valid as shown by the high degree of linear correlation (r=0.97 and 0.99) between the relative infected cell concentration (in inoculum) and the transformed cell foci counted. 6. No differences were observed between the GF classical strain and the CR-64 acute strain of Marek's disease as far as cell culture behavior. 7. Characterization of the isolates by physical and chemical treatments, development of internuclear inclusions in Infected cells, and nucleic acid typing by differential stainings and cytochemical treatments indicated that the natures of these cell transformation agents closely resemble to those described fer the group B herpes viruses. 8. Susceptible chicks inoculated with infected kidney tissue culture cells developed specific lesions of Marek's disease, and in a case of prolonged observation after inoculation (5 weeks) the birds developed clinical symptoms and gross lesions of Marek's disease. Kidney cell cultures prepared from those inoculated birds and sacrificed showed a superior recovery of cell transformation property by formation of distinct foci. 9. Electron microscopic study of infected kidney culture cells (GF agent) by negative staining technique revealed virus particles furnishing the properties of herpes viruses. The particle was measured about $100m{\mu}$ and, so far, no herpes virus envelop has been seen from these preparations. 10. No relationship of both isolates to avian leukosis/sarcoma group viruses and PPLO was observed.

  • PDF

A Study on change in thermal properties and chemical structure of Zr-Ni delay system by aging (노화에 따른 Zr-Ni계 지연관의 열 특성 및 화학적 구조 변화에 관한 연구)

  • Park, Byung Chan;Chang, Il Ho;Kim, Sun Tae;Hwang, Taek Sung;Lee, Seungho
    • Analytical Science and Technology
    • /
    • v.22 no.4
    • /
    • pp.285-292
    • /
    • 2009
  • It has been observed that, after long term storage, some ammunitions are misfired by tamping (combustionstopping) due to aging of the chemicals loaded in the ammunitions. Used in ammunitions are percussion powder which provides the initial energy, igniter which ignites the percussion powder, and a delay system that delays the combustion for a period of time. The percussion powder is loaded first, followed by the igniter and then the delay system, and the ammunitions explode by the energy being transferred in the same order. Tamping occurs by combustion-stopping of the igniter or insufficient energy transfer from the igniter to the delay system or the combustion-stopping of the delay system, which are suspected to be caused by low purity of the components, inappropriate mixing ratio, size distribution of particulate components, type of the binder, blending method, hydrolysis by the humidity penetrated during the long term storage, and chemical changes of the components by high temperature. Goal of this study is to find the causes of the combustion-stopping of the igniter and the delay system of the ammunitions after long term storage. In this study, a method was developed for testing of the combustion-stopping, and the size distributions of the particulate components were analyzed with field-flow fractionation (FFF), and then the mechanism of chemical change during long term storage was investigated by thermal analysis (differential scanning calorimetry), XRD (X-ray diffractometry), and XPS (X-ray photoelectron spectroscopy). For the ignition system, M (metal)-O (oxygen) and M-OH peaks were observed at the oxygen's 1s position in the XPS spectrum. It was also found by XRD that $Fe_3O_4$ was produced. Thus it can be concluded that the combustion-stopping is caused by reduction in energy due to oxidation of the igniter.

Analysis of Forest Change Characteristics in North Korea using Multi-temporal Satellite Images (다시기 위성영상을 이용한 북한 전체의 산림 변화 특성 분석)

  • Lee, Hyoung-Kyu;Oh, Myoung-Kwan
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.19 no.1
    • /
    • pp.633-638
    • /
    • 2018
  • We are constantly hearing about the seriousness of food shortages in North Korea through various media reports. Recently, the severity of the problem has increased, and international organizations and relief organizations have become increasingly concerned. Due to the shortage of food and firewood, residents illegally cut trees in the mountains and, as a result, North Korea has become the third fastest-growing area of forest degradation in Asia. However, since North Korea cannot directly measure the extent of forest degradation, remote sensing techniques using satellite imagery have to be applied. The purpose of this study was to analyze the characteristics of forest change in North Korea, in order to understand the severity of the forest degradation problem. For this purpose, Landsat 5 TM and Landsat 8 OLI TIRS satellite images were acquired and classified. As a result, it was found that the forests have turned into wilderness in the Nampo City and Pyongyang municipalities, while the wasteland has changed into forests in the north of Yanggangdo. In addition, the total forested area of the whole region decreased by $4,166.22km^2$, the residential area decreased by $2,017.03km^2$, and the amount of agricultural land increased by $6,625.74km^2$, which is similar to the amount of forested area lost, although the difference in the overall area of the forests between 2017 and 2006 was small.

Impact of Group Delay in RF BPF on Impulse Radio Systems (임펄스 라디오 시스템에서 RF 대역 통과 필터의 군지연 영향 분석)

  • Myoung Seong-Sik;Kwon Bong-Su;Kim Young-Hwan;Yook Jong-Gwan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.16 no.4 s.95
    • /
    • pp.380-388
    • /
    • 2005
  • This paper presents analysis results of the effects of RF filter characteristics on the system performance of impulse radio. The impulse radio system transmits modulated pulses having very short time duration and information can be extracted in receiver side based on cross-correlation between received and transmitted pulses. Accordingly, the pulse distortion due to in-band group delay variation can cause serious system performance degradation. In general, RF bandpass filters inevitably cause group delay difference to the signal passing through the filter which is proportional to its skirt characteristic due to its resonance phenomenon. For time as well as frequency domain analysis, small signal scattering parameter $S_{21}$ and its Fourier transform are used to characterize output pulse waveform under the condition that the input and output ports are matched. The output pulse waveform of the filter is predicted based on convolution integral between input pulse and filter transfer function, and resulting BER performances in the BPM and PPM based impulse radio system are calculated.

Kinetic Behavior of Immobilized Tyrosinase on Carbon in a Simulated Packed-Bed Reactor (충전층에서 탄소에 고정시킨 Tyrosinase의 반응속도에 관한 연구)

  • Shin, Sun Kyoung;Kim, Kyeo-Keun
    • Analytical Science and Technology
    • /
    • v.10 no.1
    • /
    • pp.66-74
    • /
    • 1997
  • Influence of the axial dispersion on immobilized enzyme catalytic bed was investigated in order to examine the kinetic behavior of the biocatalysis. The enzyme employed in this study was the tyrosinase(EC 1.14.18.1) immobilized on carbon support : this system requires two substrates of phenol and oxygen. This enzyme has potential application for phenol degradation in waste water. A simulated reactor was a packed-bed reactor of 2.54cm in diameter and 10cm long, loaded with the immobilized carbon particle with an average diameter of $550{\mu}m$. A phenol feed in the strength of 55.5mM(5220ppm) was used to observe the behavior of the immobilized enzyme column at three different dissolved oxygen levels of 0.08445mM(2.7ppm), 0.1689mM(5.4ppm) and 0.3378mM(9.5ppm) with the flow rates in the range of 60(1mL/s) to 180mL/min(3mL/s). Examination of the Biot number and Damkolher numbers of the immobilized system enables us to eliminate the contribution of external mass transfer to set of differential equations derived from the dispersion model. Solution of the equation was finally obtained numerically with the application of the Danckwert boundary conditions and the assumed zero-and first order rates on the non-linear two substrate enzyme kinetics. Higher conversion of phenol was observed at the low flow rates and at the higher oxygen concentration. Comparison of axial dispersion and plug flow model showed that no detectable difference was observed in the column outlet conversion between the axial and the plug flow models which was in complete agreement with the previous studies.

  • PDF

Comparisons of Empirical Braking Models for Freight Trains Using P4a Distribution Valve (P4a 분배밸브를 사용하는 화물열차의 경험적 제동모델들의 비교)

  • Choi, Don Bum;Kim, Min-Soo;Lee, Kangmi;Kim, Young-Guk
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.21 no.1
    • /
    • pp.61-69
    • /
    • 2020
  • This study examined the braking characteristics of a heavy haul freight train with P4a distribution valves applied to domestic high-speed freight trains. A freight train was composed of 50 cars, which is twice the normal operation. A braking test was performed to confirm the characteristics of the braking of a heavy haul. The brake cylinder pressures were measured for emergency and service braking on the 1st, 10th, 20th, 30th, and 50th cars. Because the brake signal is transmitted to the pressure through the braking tube connected to the end of the train, the rear vehicle is braking later than the vehicle ahead. Therefore, it is necessary to predict the brake pressures in all cars in a train to supplement the results of the limited tests and calculate the braking distance. The pressure in each car was determined using empirical models of linear interpolation, stepwise, and exponential models, which provided reliable information. The predictive results of the empirical models were compared with the measured results, and the exponential model was predicted relatively accurately. These results are expected to contribute to the safe operation of heavy haul freight trains and can be used to predict the braking distance and calculate the level of impact between vehicles during braking.

The linguistic characteristics of Chinese character and Reading for the Analects of Confucius (한자(漢字)의 언어적 특성과 『논어(論語)』 읽기)

  • Kim, Sang-Rae
    • The Journal of Korean Philosophical History
    • /
    • no.30
    • /
    • pp.191-225
    • /
    • 2010
  • This paper is the outcome of attempting to approach for reading the Analects of Confucius through the polysemy of Chinese character and indecidability of articles. For this purpose at first, I explained which this Chinese character can be applied for 'philosophy language'. In the 16th century Matteo Ricci had tried to find out the possibility of ideographic script as standing for a universal language. On the other hand, Hegel and Heidegger strictly insisted on the Chinese character is inappropriate for expressing the logic thought of the human being. The reason was as next; firstly, this character had not the preposition and articles, and secondly the only one word could not indicates the bisemy including the meaning of opposition, lastly this language system expresses and communicates only with the change of word order without inflection. But With some scholar like Cassirer, Saussure and Derrida we can confirm the possibility which will discover the Chinese character for using the logic and reasoning language of from different view. Because in the language system of this Chinese character the connection of words in contexts is more important other than meaning as the individual word, in comparison to the language of the West. The Chinese character hides the original meaning until being what kind of event and thing relationship watch inside with different letters. So to speak, the Chinese character is called as 'language of indecidability'. For these points, even though The Chinese character lacks of preposition, articles, and inflection speech etc. the letter systematic, this language system can play a role for expressing as the philosophic language which manages with the complicated problems of the human being.

Effect of Blue Color-deficient Sunlight on the Productivity and Cold Tolerance of Crop Plants (청색파장(靑色波長)영역이 결여된 태양광이 작물(作物)의 생산성(生産性) 및 내냉성(耐冷性)의 향상에 미치는 효과 Ⅰ. 광합성(光合成) 및 호흡(呼吸)의 전자전달계 활성(活性)의 변화)

  • Jung, Jin;Kim, Jong-Bum;Min, Bong-Ki
    • Korean Journal of Environmental Agriculture
    • /
    • v.5 no.2
    • /
    • pp.141-148
    • /
    • 1986
  • The blue-light effect on the grown as well as on the physiological activity of some major horticultural plants in Korea has been investigated. The light quality used for the work was obtained from sunlight filtered by an orangecolored polyethylene film which removed about 70% of visible light in the spectral region of $350㎚{\sim}500㎚$. The film was developed in this laboratory especially for the work and named BCR film meaning blue color-removing film. The light environment in the plastic house which was built with BCR film provided plants with the blue color-deficient sunlight. Thus, the photobiological effect of blue light could be examined conversely by comparing with the effect of white sunlight in a conventional plastic house built with colorless polyethylene film. In a sense of applicability to horticulture, two remarkable effects of the blue color-deficient sunlight on plant physiology were observed: First, it enhanced to a great extent the growth activity of plants-pepper, cucumber, zucchini, tomato, and leaf lettuce at the vegetative stage as well as at the reproductive stage, as demonstrated by their yield which were in average $40{\sim}50%$ increased compared with the control (under white sunlight). Second, it improved significantly the cold tolerance of plants, as exhibited with their resistance to chilling during treatment in a cold chamber maintained at a temperature which caused chilling injury to the plants of control. The visualized effects were reflected on the physiological activity of cells on organelle level. Chloroplast isolated from the plant leaves grown under BCR film showed considerably stronger photosynthetic activity, as judged by the increased electron transport rate of illuminated chloroplast, than that from leaves grown under white PE film. Mitochondria from leaves grown under BCR film maintained normal respiration activity until temperature decreased to a few degree($^{\circ}C$) lower than the temperature which caused respiratory inhibition to mitochondria obtained from leaves of the control.

  • PDF

A Study on the Dépaysement of the Animation (애니메이션에 있어서 데페이즈망에 관한 연구)

인력 수급 계획 수립을 위한 시스템 다이내믹스의 활용 - UIT도입에 따른 정보 보호 환경 변화를 중심으로 -

  • 박상현;연승준;김상욱
    • Proceedings of the Korean System Dynamics Society
    • /
    • 2003.08a
    • /
    • pp.83-108
    • /
    • 2003
  • 한 산업에서 인력 수급을 전망하는 것은 인력의 수요자인 기업의 측면에서는 안정적인 인력 확보 전략을 수립하기 위해서, 공급자인 산업 종사자들에게 있어서는 자신들이 앞으로 진출해야할 산업의 매력도를 파악하기 위해서, 그리고 정부 차원에서는 관련 산업에 있어서 중복 투자의 방지와 효율적이고 균형 된 산업 발전을 위한 정책 수립을 위해서 매우 중요하다. 그러나 이러한 인력 수급 전망들은 종종 잘못된 시장 분석으로 인하여 인력의 과소 공급 또는 과잉 공급이라는 의도하지 않은 결과를 가져오는 경우가 있다. 이는 전체적인 시각에서 시장의 구조적 특성을 분석하기보다는 현상을 조사하는 수준에 머물거나 현재의 상황 또는 단일 산업만을 고려할 뿐 시간의 흐름에 따른 동태적 변화와 지연된 피드백의 효과, 그리고 관련 산업간의 유기적 연관관계를 반영하지 못한 채 단기적이고 단선적인 관점에서 인력 수급을 전망하는데 그 원인이 있다고 볼 수 있다. 특히, 다른 산업과의 연관 관계가 복잡하고 인력의 수요의 급증에도 불구하고 산업에서 요구하는 인력을 양성하기까지 많은 시간이 소요되는 첨단 산업 및 신생 산업에서의 경우 이러한 현상은 더욱 두드러지게 나타날 수 있다. 이러한 관점에서 본 논문은 변수간의 상호 동태적인 관계와 시간의 흐름에 따른 행태를 분석하는 데 용이한 SD 방법론에 기초하여 최근 빠르게 성장하고 있는 정보보호산업에서의 동태적인 인력 수급 모델을 구현하여 향후 국내 정보 보호 인력의 수급 행태가 어떻게 전개될 것인지를 분석해 보았으며 이를 통하여 동태적 시각에서 인력 수급 불균형 현상의 원인을 파악하고 문제 해결을 위한 대안을 제시하고자 한다.채취하여 임신진단키트(제네디아프로테 트, 녹십자)를 이용하여 임신여부를 1차적으로 확인하였다. 과배란을 유기한 13두의 공란우중 9두(69.2%)가 과배란 반응을 나타내었으며, 회수된 수정란 51개중 이식가능수정란은 38개(74.5%) 였다. 발정동기화를 유도한 수란우 40두중에서 35두(87.5%)가 발정이 동기화되었으며, 그 중 황체검사를 통하여 30두의 수란우에 수정란을 이식하였다. 수정란이식후 13일(발정주기 21일)에 혈액을 이용한 임신진단에서 농가별 수태율은 각각 37.5%, 70.0%, 60.0% 및 71.4% 로서 평균 60.0%를 나타내었다.서 39$^{\circ}C$, 5% $CO_2$ 배양기에 48시간 배양하면서 생존여부를 판단하였다. 실험 2에서 확장배반포배 수정란이 25.3%의 생존율을 나타내었으며, 실험 1과 실험 3에서는 수정란의 형태와 관계없이 생존성을 확인할 수 없었다. 이상의 결과로 보아 glycerol 완만동결에서는 확장배반포기 수정란 이상이 보존가능한 것으로 추정되나 더 추가적인 연구가 요구된다.c kinase 활성의 변동은 정소 내 간충조직, 세정관 상피의 증식 및 기능적 분화 과정을 매개하는 생리적 활성분자 수용체 하위의 신호전달 과정에 Src-Csk loop에 의한 조절가능성을 확인할 수 있었다.rugrene의 향기성분이 주요 성분군으로 확인되었다. 2. 생강나무에서 생강의 향기를 발산하는 성분으로는 $\beta$-myrcene, o-terpinolene, phellandrone, ι-limonene, $\beta$-eudes

  • PDF