• Horticultural Science & Technology
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• v.19 no.3
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• pp.338-341
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• 2001

A new passive nutrient supplying system (PNS) was designed. The experiment was conducted to compare PNS with automatic hydroponic system (AHS) by investigating the growth characteristics of muskmelon (Cucumis melo L.) in spring and summer. No significant differences in growth characteristics of leaf area and shoot dry weight were observed between PNS and AHS. However, better netting of melon fruit was shown in PNS. Sugar content of melon fruit was also $1.0-2.0Brix^{\circ}$ higher in PNS than in AHS. The suitable substrate for melon culture with PNS was the mixture of perlite 70% and rice hull 30%. These results suggested that new PNS could be introduced to growers without any loss of fruit yield and quality of muskmelon.

• Proceedings of the Korean Journal of Food and Nutrition Conference
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• 2003.07a
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• pp.82-82
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• 2003

오디는 옛부터 보혈자음(補血滋陰), 생진윤조(生津閏操), 현훈이명(眩最耳鳴), 심계실면(心悸失眠), 수발조백(鬚髮早白) 등을 치료하는 효능을 가진다고 알려져 있다. 상심자(桑 子)라 하여 뽕나무 열매로서 한방에서 상심(桑 ), 상실(桑實), 오심(烏 ), 흑심(黑 ) 등으로 지칭되며, 뽕나무과(Moraceae)에 속하는 뽕나무(Morus alba L)의 성숙한 과실로 취화과(聚花果)에 속하며 작은 수과(瘦果)가 많이 모여 이루어진 장원형으로 길이 1 내지 2 cm, 지름 0.5 내지 0.8 cm이며 황갈색, 갈홍색 또는 암자색을 띠고 짧은 줄기가 있다. 작은 수과(瘦果)는 난원형으로 조금 납작한 편이며, 길이는 약 2 mm, 너비는 약 1 mm 이고 육질의 화편(花片) 4개가 둘러싸고 있다. 그러나 아직까지는 오디에 대하여 자연과학적인 연구방법을 이용하여 구체적으로 연구된 바가 많지 않다. 또한 이의 생리활성 성분에 대해서도 그 작용과 연관지어 보고된 바가 많지 않다. 뇌졸중 가운데 뇌조직으로 가는 혈액 공급의 감소 혹은 차단으로 발생되는 허혈성 뇌졸중(ischemic stroke)은 전체 뇌졸중 환자의 약 80%정도를 차지하고 있으나 아직까지 뇌신경세포 손상기전의 복잡성 등으로 뇌졸중으로 발생하는 뇌신경세포의 손상을 보호해 줄 수 있는 물질이 개발되어 있지 못한 실정이다. 한편, 천연물로부터 뇌허혈 보호작용을 가지는 물질의 도출은 주로 한방에서 처방을 중심으로 이루어지고 있으며, 따라서 처방으로부터 신경보호작용을 가지는 물질의 도출은 그 처방에 함유되어 있는 각종 생약이 갖는 다양한 활성으로 인해 어려운 점이 있으며, 비록 효과가 있다고 하더라도 과학적인 입증자료가 매우 미비한 실정이다. 이에 본 연구에서는 위에 언급된 자료를 토대로 오디추출물이 뇌허혈에 효과가 있을 것이라는 가정하에 in vitro system을 이용하여 오디추출물의 신경보호작용기전을 검색하고자 DPPH radical 의 생성억제효과, PC12 cell line을 이용한 산화적 스트레스에 의한 오디추출물의 방어효과, LSP에 의하여 활성화된 BV-2 cell에 미치는 영향 등을 검색하였다. 오디의 DPPH 소거활성은 단일화랍물인 bacicalein과 유사한 효과를 나타냈으며 PC12 cell line을 이용한 산화적 스트레스에 의한 오디의 방어 효과는 LDH activity를 행한 결과 농도 의존적으로 LDH 유리 량을 감소시켰다. 뇌손상시에 오디추출물의 염증방어 효과를 관찰하기 위하여 microglial cell line인 BV2 세포주를 선택하여 배양한 후 LPS로 자극을 준 후 일차적인 염증지표인 NO양을 측정한 결과 BV2 cell에 LPS 100 ng/ml을 처리하는 경우 nitrite량이 유의적으로 증가하였는데 이때 오디추출물을 1, 5, 10 ug/ml의 용량으로 처리하는 경우 역시 저농도에서 NO생성량을 감소시켰다. 정리해보면 뽕나무과 식물인 오디는 신경보호효과가 있는 것으로 사료되는데 이러한 효과는 아마도 오디의 항산화효과에 기인한 것으로 추정된다.

• Research in Plant Disease
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• v.19 no.2
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• pp.124-127
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• 2013

Black necrotic spots were observed from the fruits of paprika that were cultivating in a vinylhouse. The casual agents of the symptom were identified as several isolates of Pepper mild mottle virus (PMMoV) by responses of indicator plants, electron microscopy, and RT-PCR analysis. Symptoms of the viral disease were mild mottle in the young leaves, necrotic spots on the fruits and the fruit apex of paprika, but the symptoms were not shown on the mature leaves. All of the PMMoV isolates were determined as $P_{1.2.3}$ pathotypes from the biological responses on the chilli pepper lines used for discrimination of tobamovirus pathotypes. Pathogenicity of the PMMoV isolates was also confirmed using mechanical inoculation method to paprika seedlings. The coat protein (CP) genes of the PMMoV isolates were compared at the nucleotide and amino acid levels with the previously published PMMoV isolate. The isolates share 96 to 99% CP nucleotide identity among the isolates. The CP of $P_{1.2}$-pathotype PMMoV-P2 presented Met at position 139, But the CPs of $P_{1.2.3}$-pathotype PMMoVs from paprika showed Met to Asn substitution at the same position. This is the first report of identification of $P_{1.2.3}$-pathotype PMMoV isolates from paprika in Korea.

• Research in Plant Disease
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• v.19 no.3
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• pp.226-228
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• 2013

In 2012, a pink mold rot was observed on unishiu orange (Citrus unshiu Mac.) fruits at the Wholesale Market for Agricultural Products, Jinju, Korea. The symptom on unishiu orange was a water-soaked lesion on the surface of fruit, which later on enlarged to form softened brown rot lesions. The diseased fruits were covered with pink-colored mold, consisting of conidia and conidiophores of the pathogen. Optimum temperature for mycelial growth was $25^{\circ}C$. Conidia were hyaline, smooth, 2-celled, and thick-walled conidia with truncate bases, ellipsoidal to pyriform, characteristically held together zig-zag chains and $12-26{\times}8-12{\mu}m$ in size. Conidiophore was erect, colorless, unbranched, and 4-5 ${\mu}m$ wide. On the basis of mycological characteristics, pathogenicity test, and molecular analysis with complete ITS rDNA region, the causal fungus was identified as Trichothecium roseum (Pers.) Link ex Gray. This is the first report of pink mold rot caused by T. roseum on unishiu orange in Korea.

• Korean Journal of Plant Tissue Culture
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• v.28 no.5
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• pp.283-287
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• 2001

Differential display based on PCR was employed to identify genes expressed during tuber-developing stage of potato (Solanum tuberosum L. cv. Irish Cobbler). An eukaryotic initiation factor 5A (eIF-5A) clone isolated from a cDNA library constructed with developing micro-tuber using a probe of PCR fragment. We isolated three positive clones and ore of them contained open reading frame. This clone revealed high sequence similarity to tomato eIF 5A cDNA. At the DNA level, there is 94.8% identity with the tomato eIF-5A4, whereas at the protein level there is a high identity with 97.5%. The potato eIF 5A clone is 716 bp in length and contains a single open reading frame from 57 to 539 bp, a 56 bp 5'-untranslated region and a 177 bp 3'-untranslated region. The deduced protein composed of 160 amino acid residues, with a predicted molecular mass of 17.4 kD and an estimated pl of 5.5. The sequence of 12 (STSKTGKHGHAK) amino acids among eIF-5A proteins is perfectly conserved from yeast to human. That sequence in potato eIF-5A protein is also conserved at position 46 to 57 amino acid. This region embeds the post-translational modification site of the lysine residue (at the seventh K) to hypusine that is crucial to eIF-5A activity. The northern blot analysis of eIF5A has shown abundant expression, mainly in flower organs (stamen, ovary, petal, sepal), fruit and stolen.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.16-25
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• 2015

This research was conducted to analyze flavonoids in juice and to investigate correlation between 60 germplasms of satsuma mandarin (Citrus unshiu Marc.) on the basis of the result of flavonoid analysis. Juice of satsuma mandarin contained 6 flavonoids, 2 flavanones such as narirutin and hesperidin, 1 flavone such as rutin and 3 polymethoxylated flavones such as sinensetin, tangeretin and nobiletin. Hesperidin content ranged from 41 to 196 mg/L. Narirutin content ranged from 25 to 230 mg/L. The average of rutin content was 2.2 mg/L but it was not detected in some cultivars. Polymethoxylated flavone which is known as a citrus specific flavonoid, was detected in all germplasms in small quantities. Cluster analysis using R program (Version 3.1.1) was carried out with the results of the flavonoid analysis and harvesting time of satsuma mandarin germplasms. All germplasms were grouped as A, B1 or B2. But all 3 groups contained very early-ripening type, early-ripening type and commonly-ripening type cultivars. This showed there was no correlation between flavonoids in juce and their harvest time.

• Proceedings of the Korea Database Society Conference
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• 1998.09a
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• pp.509-524
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• 1998

정보시스템 개발노력의 상당수는 실패로 끝나고 있다. 최근 통계에 따르면 정보시스템 개발 프로젝트의 반은 실패로 끝난다고 한다［kaplan, 1998］. 이와 같은 높은 실패율은 시스템 개발을 위한 노력을 체계적으로 투입하지 못하고 개발 프로젝트를 관리하기 위한 노력을 단위프로젝트 차원에서만 집중시키는데서 연유한다고 생각된다. 다시 말해 장기적인 관점에서 개발조직의 역량 향상이라는 보다 근본적인 목표를 간과하고 있는 것이다. 이러한 점에 착안하여 소프트웨어 엔지니어링 분야에서는 정보시스템 개발과 관련'한 개발 조직의 능력을 향상시키기 위한 다양한 접근방법이 제시되고 있다. 개발조직의 개발 프로세스 성숙도를 진단하기 위한 측정도구로 개발된 카네기멜론대학의 CMM(Capability Maturity Model)과 ISO 에서 정의한 표준인 SPICE (Software Process Improvement and Capability dEtermination) 모델이 그 대표적인 예에 속한다. 그러나 이와 같은 모델들은 개발조직의 프로세스 개선을 위한 방향과 요건은 제시하고 있지만, 이를 조직 내에서 구현하기 위한 구체적인 방법이나 수단은 제시해주지 못하고 있다. 따라서 이러한 접근방법 역시 소프트웨어 엔지니어링 역량 이나 개발경험이 일천한 우리 현실에서는 부분적인 성과 이상을 기대하기는 어려웠다. 본 연구에서는 이와 같은 문제점이 개발 프로젝트와 관련된 경험이나 지식을 효과적으로 추출하고, 획득하고, 체계화하고, 시스템화하여 조직 내에서 활용하려는 노력이 부족한기 때문이라고 본다. 이에 본 연구에서는 개발조직의 역량 향상을 위한 지식관리적 접근 방법의 세가지 유형을 제시하기로 한다.>$Ca^{2＋}$ 는 뿌리에서, $Mg^{2＋}$ 는 잎에서 많았으며, $PO_4$$^{-}$ 는 과실과 줄기에서 많았다. 배지간에 따른 차이는 나타나지 않았으며, $K^{+}$, $Ca^{2＋}$ 및 $Mg^{2＋}$ 는10:0에서, $PO_4$$^{-}$ 는 8:2에서 각각 많았다.해 제품을 판매하였으며, 기업 및 제품이미지 제고를 위한 고객에 대한 서비스도 강화하고 있었다. 통신기기업체내지 소프트웨어 산업으로의 진출이 가능할 수 있도록 상호진출을 허용할 필요가 있다고 본다. 이를 위해서 우리 나라 정부 역시 미국처럼 새로운 통신개혁법을 만들 필요가 있다. 새로운 통신개혁법의 핵심적인 사항으로서 첫째, 통신과 CATV간의 상호진입을 허용, 둘째, 통신사업자가 통신관련 기기산업에 참여할 수 있는 규제완화를 허용, 셋째, 유아단계에 있는 소프트웨어 및 컨테트산업을 육성하는데 산업육성책 수립 등을 적극적으로 추진하여야 할 것이다. 그리고 현재 국내 재벌기업들로 구성되어 있는 기반산업을 지원하는 기술개발 지원체제와 육성정책을 소프트웨어 및 컨텐트의 응용산업으로 개편할 필요성도 제시되며, 이를 위해 범부처 차원에서 소프트웨어 및 컨텐트 육성정책을 지원하는 종합적인 대책을 마련해야 한다고 본다.서, Li-K, Li-Na탄산염에 대하여 부 식거동을 검토한 결과, 가압하에서 내식성이 향상되는 것이 발견되었다. 이유로서는 가압하에서 용융탄산엽의 증가된 산화력으로 보다 치밀한 내식성 산화물 피막이 형성되기 때문으로 생각되고 있다. 또

• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.319-324
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• 2008

The sweet pepper (paprika) is one of the most important exported vegetable crops in Korea. This study evaluated the storability of sweet pepper fruits between 2 cultivars with different maturities under different storage conditions. The sweet peppers ('Special' and 'Fiesta') were grown in a rockwool hydroponic system, and harvested two different maturities: half-maturity and full-maturity levels. The sweet pepper fruits were stored in MA(non-ventilated) and non-MA(ventilated) conditions at 4 and $9^{\circ}C$ for 20 days. The storability of sweet pepper fruit was the highest in $4^{\circ}C$ MA conditions that remained 5% carbon dioxide and 10% oxygen during the storage. The ethylene concentration in MA condition showed $2{\sim}8{\mu}l/l$ regardless of storage temperatures, cultivars and maturities. The fruits packaged with non-ventilated film, showed lower weight loss, and higher firmness, and there was no different about decay ratio compared to the fruits packed ventilated film. The coloration of semi-matured fruit that colored 50% before storage progressed faster in non-ventilated packaging condition at $9^{\circ}C$, but their color did not changed as same as level of full-matured fruit. The electrolyte leakages and respiratory rate that estimated degree of chilling injury was highest in non-MA(ventilated) conditions of 'Special' full matured fruit placed at room temperature for 3 days following storage at $4^{\circ}C$ for 20 days. It may be suggested that sweet pepper fruit packed sealed (non-ventilated) film and stored at non-chilling temperature can be maintained better quality for long term storage.

• Korean Journal of Environmental Agriculture
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• v.27 no.2
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• pp.178-184
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• 2008

This work was carried out to investigate the effect of waterlogging on the growth and nutrient contents of 'Campbell Early' and 'Kyoho' grapevines under the vinyl house condition from June 14 to July 20, 2005. For the trial, seedlings of two-year-old grapevine were transplanted to 40 L pot with a sandy loam soil. Irrigation point of non-waterlogging(control) treatment was controlled at -40 kPa of soil water tension using tensiometer and waterlogging treatments were imposed for 35 days at the water levels of above 10 cm from the soil surface using tap water. The growth of aerial(shoot length, leaf number and stem diameter) and underground(root) parts of 'Campbell Early' and 'Kyoho' grapevines tended to be wholly reduced by waterlogging, while the growth of aerial parts were more severely impaired in 'Kyoho' than in 'Campbell Early' cultivar. The different responses for waterlogging between two grapevines seem to be related with the capacity for absorbing mineral nutrients, because nitrogen content of 'Campbell Early' cultivar leaves was significantly higher than that of 'Kyoho' cultivar although the contents of phosphorus and potassium in leaves of two grapevine cultivars were similarly declined. There was no significant different of fruit quality, such as contents of soluble solid, titratable acidity and weight of berry in 'Campbell Early' between waterlogging and control. In 'Kyoho' cultivar, however, berry weight and titratable acidity were decreased and soluble solid content was increased by waterlogging. It was assumed that waterlogging stress for grapevines promotes maturation and coloring processes of berries by stimulating maturation hormone such as ethylene. In conclusion, 'Campbell Early' cultivar seems to be more tolerable than 'Kyoho' cultivar when comparing the growth responses and nutrient contents between two grapevine cultivars under waterlogging.

• Korean Journal of Agricultural and Forest Meteorology
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• v.19 no.2
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• pp.43-53
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• 2017

This study aims to analyze the effect of typhoons on pear production. Pears are typical fruits that are vulnerable to typhoon damages, so typhoons are negatively associated with pear productivity. However, relatively less pear damages by typhoons in the main pear production area, comparing to the average in Korea, have been reported. The main production area seems to adopt better agricultural techniques or practices to cope with natural disasters such as typhoons. Thus, this study tests the hypothesis that there are differences of production losses due to typhoons between the main pear production area and the rest using the stochastic frontier analysis. The main production area is defined by Location Quotient Index (LQI), and we found that LQI had a significant effect to decrease the productivity losses in the main production areas, which shows that those production areas alleviated the pear production loss due to typhoons.