• Korean Journal of Fisheries and Aquatic Sciences
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• v.25 no.3
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• pp.181-188
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• 1992

To study the effect of alginic acid on modulation of the aging process, Sprague-Dawley(SD) male rats were fed the diets containing 0, 3, 6 and $9\%$ alginic acid isolated from brown algae(Undaria pinnatifida) for 16 weeks. The effects of alginic acid on body weight, malondialdehyde(MDA) content, peroxidizability index, cholesterol and phospholipid levels, cholesterol/phospholipid(Ch/Ph) molar ratio, and fatty acid compositions in liver membranes were investigated. Increasing alginic acid level in diets did not alter food intakes but effectively decreased body weights gain(p<0.01-0.005). Malondialdehyde(MDA) contents of diets containing 6 and $9\%$ alginic acid were effectively decreased in ranges of $54.1-43.0\%$ in mitochondria, and $65.5-87.7\%$ in microsome compared with $100\%$ of control group. Cholesterol levels of all diets containing alginic acid were significantly decreased in ranges of $87.0-72.3\%$ in mitochondria, and $87.4-68.1\%$ in microsome compared with $100\%$ of control group. Phopholipid levels in microsome were significantly decreased by diets containing 3 and $ 6\%$ alginic acid but Ch/Ph molar ratios in both membranes were decreased by diets containing 3 and $6\%$ alginic acid. Increasing alginic acid level in diets significantly decreased total fatty acid but effectively increased linoleic acid in microsome except for diet containing $9\%$ alginic acid. These data on liver membranes suggest that alginic acid added to diets can modulate the physiological changes if the aging process.

• Microbiology and Biotechnology Letters
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• v.26 no.6
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• pp.558-561
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• 1998

In the screening for lipid peroxidation inhibitors from edible mushroom, Agrocybe cylindracea, a bioactive compound AG 8 was isolated. The AG 8 was purified from methanol extract of its fruit body by Diaion HP-20 column chromatography, ethyl acetate extraction, and silica gel column chromatography, consecutively. Based on various NMR studies including $^1$H irradiation and HMBC experiments, the AG 8 was identified as MTA, 5'-deoxy-5'-methylthioadenosine. This compound inhibited lipid peroxidation with an $IC_{50}$/ value of 3.2 $\mu\textrm{g}$/$m\ell$. The MTA was isolated for the first time from basidiomycetes.

• Journal of Life Science
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• v.11 no.4
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• pp.328-334
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• 2001

Male Sprageu-Dawely rats fed a cholesterol diet (Control group) or cholesterol diet supplemented with the water-soluble extract of stem bark from Cudrania tricuspidata(CTSB group) at the level of 0.5% for 2 weeks. Concentration of triglyceride in serum of CTSB group was significantly lower than that of control group. However, the other lipids concentrations of liver and serum, body weight gain, and food intake did not show significant difference between CTSB group and control group. Antioxidative activities of water-soluble extract from stem bark of Cudrania tricuspidata on the lipid peroxidation in rat were also studied in vivo by measuring the formation of thiobarbituric acid reactive substance(TBARS). Concentration of TBATS in the liver homogenates of CTSB group was significantly higher than that in control group. Nonheme iron concentration was significantly increased in the liver of CTSB group compared to control group. suggested that enhanced nonheme irom was associated with enhanced peroxidation of liver in CTSB group. These results suggested that water soluble extract from stem bark of Cudrania tricuspidata exert the hypotriglycerolemic effect and might amplify the lipid peroxidation of tissues in cholesterol fed rats.

• The Journal of Internal Korean Medicine
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• v.22 no.1
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• pp.21-27
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• 2001

목적 : 본 연구는 배기음(排氣飮)이 토끼의 위장관내에서 화학물질에 의해 유발된 장관의 궤양에 유효한 효과를 발휘할 수 있는지를 검증하기 위한 실험이다. 방법 : 토끼 5마리를 한 군으로 하여 정상군과 체중 1kg당 200mg 분량의 mepirizole을 경구 투여한 군과 100mg/kg의 배기음(排氣飮)(경구투여)과 800Units/kg 분량의 catalase(정맥주사)를 mepirizole을 경구투여하기 2시간 전에 각각 전처치한 군으로 나누었다. Mepirizole을 경구 투여한 후 각각 24hr와 48hr에 토끼를 희생시켜 위장, 십이지장부의 궤양성 병변을 관찰하였다. 결과 : Mepirizole을 경구투여하여 위장 및 십이지장 기부의 궤양성 병변이 유발되었다. 배기음(排氣飮)(경구투여)과 catalase(정맥주사)를 전처치하였을 경우 궤양의 크기가 현저하게 줄어들었다. Mepirizole은 십이지장 점막에서 지질의 과산화를 증가시키는데 이는 수산화기와 관련되어 있음을 시사한다. 배기음(排氣飮)과 catalase를 전처치함으로써 mepirizole에 의해 유발된 지질의 과산화가 현저하게 억제되었다. 형태학상의 연구에서도 mepirizole의 처치에 의한 십이지장의 손상과 배기음(排氣飮)에 의한 방지효과가 나타났다. 결론 : 이러한 결과들로 볼 때 반응성산소기는 mepirizole에 의해 유발된 위장관 궤양의 병리변화 형성에 주요한 영향을 미치며 배기음(排氣飮)이 항산화작용을 통해 궤양의 형성을 억제하는 역할을 하고 있음을 나타낸다. 따라서 본 연구는 배기음(排氣飮)이 반응성산소기에 의해 매개된 인체 위장관질환에 치료적 역할을 할 수 있음을 제시하고 있다.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.04a
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• pp.103.1-103
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• 2003

건조 생약재의 위생화 수단으로 방사선 조사 기술의 적용 가능성을 검토하기 위하여 감마선 조사 생약의 효능 변화유무를 평가하고자 하였다. 본 연구에서는 감마선 조사 시료와 비조사 시료가 생체의 산화적 손상을 억제하는 효과를 비교하기 위하여 방사선에 의한 산화적 손상에 대한 효과를 측정하였다. 감마선 조사(10 kGy) 생약재(H-113) 및 비조사 생약재(H-113) 추출물을 처리하여 배양한 사람 림프구에 방사선을 조사한 후, 단세포전기영동(single-cell gel electrophoresis, SCGE; comet assay)을 수행하여 DNA 상해 경감정도를 관찰하였다. 또한 방사선 조사 및 비조사 생약재(H-113) 추출물을 투여한 생쥐에 8 Gy의 감마선을 조사한 후, 간에서 지질과산화 정도를 비교·관찰하였다. 한편 DPPH 라디칼과 hydroxyl 라디칼 소거효과를 시험관내에서 상호 비교하였다. 감마선 조사 생약재(H-113)는 단세포전기영동, 지질과산화, DPPH 및 hydroxyl 라디칼 소거시험에서 비조사 생약재 (H-113)와 유사한 효과를 나타내어 효능 차이가 인정되지 않았다. 이는 생약재의 여러 가지 고유 효능 중 일부의 안정성을 확인한 것으로 생각되며, 이러한 결과를 바탕으로 감마선 조사 생약재의 고유 효능의 안정성에 관한 체계적인 연구결과를 얻는다면 생약재의 위생화 수단으로 감마선 조사 기술의 이용이 실용화될 수 있을 것으로 사료된다.

• Korean Journal of Acupuncture
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• v.22 no.4
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• pp.83-90
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• 2005

Objectives : To investigate the effects of acupuncture at Gansoo (B-18) and Chungwan (CV-12) on lipid metabolism in rats fed peroxidized lipid diet. Methods : Effects of acupuncture at Gansoo and Chungwan on plasma and liver lipid composition and antioxidative capacity were investigated in rats fed peroxidized lipids. Results : Although the level of plasma total cholesterol and triglyceride showed a tendency to decrease in the acupuncture group, the plasma HDL-cholesterol concentration showed no significant difference. The level of liver total cholesterol and triglyceride showed no significant difference in all treatment groups. Thiobarbituric acid( TBARS ) values in plasma and liver showed a tendency to decrease in the acupuncture groups. The plasma GOT and GPT activities showed low values in the acupuncture groups. The liver glutathione peroxidase, superoxide dismutase and catalase activity showed high values in the acupuncture groups. Conclusions : The results suggest that acupuncture at Gansoo (B-18) and Chungwan (CV-12) may have an influence on lipid metabolism via enhancing antioxidative capacity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.6
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• pp.823-832
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• 1993

This study was made to determine alleviating effects of vitamin C(Vt C) on the gramoxone toxicity in rats with respect to the growth gain, feed effeciency ratio, total lipid contents, lipid peroxication and protein patterns in rat liver. Growth gain, feed efficiency ratio and liver weight in the gramoxone group were decreased significantly as compared with the control group, while those values obtained in the gramoxone-Vt C group were increased as compared with the gramoxone group. Lipid contents and TBA value of rat liver of the gramoxone group were increased significantly as compared with the control group. However, those values of the gramoxone+Vt C group were decreased as compared with the gramoxone group. No significant differences were observed in the liver protein patterns between the gramoxone and the control group. The contents of liver protein were decreased significantly in the gramoxone group fed for 2 weeks as compared with the control group. The change of the liver protein patterns, such as the decrease of high molecular weight protein and the increase of low molecular weight protein were observed in the gramoxone+Vt C group.

• Journal of Nutrition and Health
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• v.33 no.5
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• pp.502-506
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• 2000

Effects of powdered siho(bupleuri radix) on serum and liver lipid composition and antioxidative capacity were investigated in rat fed high oxidized fat. Sixty male Sprague-Dawley rats weighing 161.25$\pm$2.51g were blocked into four groups according to body weight and raised seven weeks with basal diet(normal group), basal diet and 10% oxidized fat(control group), basal diet, 10% oxidized fat and 2% powdered siho(2% powdered siho group) and basal diet, 10% oxidized fat and 3% powdered siho(3% powdered siho group). Feed intake, body weight gain and feed efficiency were not significantly different among oxidized fat diet groups. The values of total cholesterol and triglyceride in plasma and liver showed no significantly different(p>0.05) in the oxidized fat diet groups. Plasma HDL-cholesterol showed atendency to increase in powdered siho diet groups. The values of thiobarbituric acid in plasma and liver were lower in the powdered siho diet groups than control group. Plasma GOT and GPT activity showed a tendence to decrease in powdered siho groups. Liver glutathione peroxidase activity showed a tendence to increase in powdered siho diet groups. Thus, it seems that powdered siho had some antioxidative elements.

• Journal of Food Hygiene and Safety
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• v.22 no.3
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• pp.213-217
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• 2007

LPS induces the synthesis of several inflammatory cytokine, chemokine, NO and inflammation in the liver of rats. The purpose of this study was to investigate the preventive effects of Rubus coreanum Miquel (RCM) In lipid metabolism. RCM of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5ml/kg for 20 days. On the day 21, 1.5ml/kg of LPS was injected 4 hours before anesthetization. We examined the lipid-related functions by measuring the levels of triglyceride (TG), total cholesterol (TC), total lipid (TL), high-density lipoprotein cholesterol (HDL-C) in serum and malondialdehyde(MDA) in liver tissue. The results showed that LPS treatment increased the values of TG, TC, TL and MDA, decreasing that of HDL-C. But RCM pretreatment decreased the high values of TG, TC, TL and MDA to the low values and increased the low value of HDL-C to the high value. These results suggested that RCM could be used as the potential candidate for the lipid metabolism natural supplement.

• The Korean Journal of Food And Nutrition
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• v.17 no.4
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• pp.347-355
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• 2004

This study was undertaken in order to evaluate effects of methanol extracts of Stachys sieboldii MIQ and its related enzyme activities in brain tissues of rats. Sprague-Dawley(SD) male rats were fed within a control group, which is a basic diet group. The experimental diet group was given 100 and 200 mg/kg to supervise 100 and 200 mg/kg body weight per day for 20 days. Lipid peroxide levels and acetylcholine esterase activity in brain tissues were slightly decreased at a dose dependent manner, in vitro. Lipid peroxide levels were also decreased at a dose dependent manner; methanolic extracts of Stachys sieboldii MIQ demonstrated significant inhibitory effects, in vivo. Monoamine oxidase and xanthine oxidase activities were significantly inhibited in the brain tissues of experimental group compared to control group and the ratio of type conversion of xanthine oxidase were decreased.