• Applied Biological Chemistry
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• v.32 no.1
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• pp.23-29
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• 1989

Biochemical consequence of the accumulation in cells of superoxide $(O^{-}_{2})$ which was proposed to be probably a common chemical factor in the secondary process of the mechanism of chilling injury as well as in the visible light photodamage in cells of higher plants, has been investigated in the present work. Especially focused was the destructive effect of $O^{-}_{2}$ on the biochemical activity of mitochondria, as informations which support the suggestion that mitochondrial inner membrane is the major site of $O^{-}_{2}$ production have been collected. Mitochondria and submitochondrial particles (SMP) were prepared from soybean hypocotyls for this case study. When SMP were treated with the electrolytically produced $O^{-}_{2}$ they suffered not only inhibition of the membrane-bound enzymes as demonstrated by cytochrome c oxidase, but also lipid peroxidation of membrane as proved by malondialdehyde production. Malate dehydrogenase present in the protein extract from mitochondrial matrix was also inhibited by the $O^{-}_{2}$ treatment. These results exhibited the chaotic effect of the overproduction and accumulation of $O^{-}_{2}$ in cells under a certain abnormal circumstance such as environmental stress on the physiological function of mitochondrial; disruption of the cellular metabolic pathways and the structural integrity of membrane.

• Applied Biological Chemistry
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• v.38 no.2
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• pp.111-117
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• 1995

In Escherichia coli, CRP forms a complex with cAMP and acts as a transcriptional regulator of many genes, including sugar metabolism operons. The E. coli MK2001, which is introduced the altered crp, is functional in the expression of lac, ara and man, in the absence of cAMP. However, the expression of mal gene is fully activated by the addition of cAMP or cGMP. The object of the study is cloning of the sfs (sugar fermentation stimulation) genes, which was involved in regulation of mal gene expression with the altered crp gene, and structural analysis and characterization of the genes at the molecular level. We have cloned 5 different E. coli genes which stimulate the maltose metabolism in a crp, cya::km (MK2001) background. Newly identified genes were designated as sfs. One of the sfs genes (pPC1), located at the 53.2 min map position on the E. coli chromosome, was further analyzed. Expression of the genes, which is involved in maltose metabolism, malQ (amylomaltase), was increased to 5.8-fold in the presence of a plasmid, pAP5, containing the subcloned sfs4 gene. The nucleotide seguence of a common 2,126 bp segment of the pPCM1 was determined and two open reading frames (ORF1 and ORF2) were detected. The ORF1 encodes the sfs4 gene and ORF2 encodes a truncated protein. Potential CRP binding site is located in the upstream of the putative promoter in the regulatory region. Expression of the cloned sfs4 gene was positively regulated by the cAMP-CRP complex.

• KSBB Journal
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• v.24 no.3
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• pp.259-266
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• 2009

AfsR2 in Streptomyces lividans, a 63-amino acid protein with limited sequence homology to Streptomyces sigma factors, has been known for a global regulatory protein stimulating multiple antibiotic biosynthetic pathways. Although the detailed regulatory mechanism of AfsK-AfsR-AfsR2 system has been well characterized, very little information about the AfsR2-dependent down-stream regulatory genes were characterized. Recently, the null mutant of afsS in S. coelicolor (the identical ortholog of afsR2) has been characterized through DNA microarray system, revealing that afsS deletion regulated several genes involved in antibiotic biosynthesis as well as phosphate-starvation. Through comparative DNA microarray analysis of afsR2-overexpressed S. lividans, here we also identify several afsR2-dependent genes involved in phosphate starvation, morphological differentiation, and antibiotic regulation in S. lividans, confirming that the AfsR2 plays an important pleiotrophic regulatory role in Streptomyces species.

• Korean Journal of Agricultural Science
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• v.36 no.1
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• pp.27-40
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• 2009

Six strains of Phycomyces blakesleeanus (DEL, A56, C9, C47, C111, and G5) were cultured in continuous light condition for 6 days. The increase in fresh and dry weight after the culture period was similar in all strains. However, the increase in fresh weight was sustained throughout the culture period in all strains, whereas the increase in dry weight was prominent only during the first 3 days of culture. The development and phototropism of macrophores was observed after 3 days of culture, These results suggest that the development and phototropism is ascribed to the increase in turgor pressure in sporangiophores where water entered. A56 and G5 showed a higher sensitivity to light than C9 and C47, whereas C111 showed no photosensitivity. Antagonistic relations were observed between photosensitivity and development of microphores, suggesting that the development and phototropism of macrophores is regulated by a common recognition factor (photoreceptor).

• The Korean Journal of Food And Nutrition
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• v.20 no.1
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• pp.9-13
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• 2007

In this study, sesame oils and sesame meals(A, B) were obtained from roasted sesame at $200^{\circ}C$ and $220^{\circ}C$, respectively. The total volatile component(VC) contents of these samples(A, B) were as follows : The sesame oils had 1,397.1 ppm and 1,518.8 ppm, and the sesame meals had 663.2 ppm and 775.3 ppm for samples A and B, respectively : sesame flavor was16,507.9 ppm, The major VCs in the sesame oils were pentane, 2-butanone, pyrazine, methylpyrazine, 2,5- and 2,6-dimethyl pyrazine, furfuryl alcohol, guaiacol and 4-vinylguaiacol. The pyrazine contents of the seasame oils were 834.4 ppm and 816.4 ppm for samples A and B, respectively. The major VCs in the sesame meals were 2-butanone, hexanal, pyrazine, methyl pyrazine, 2,6-dimethyl pyrazine, furfuryl alcohol, and so forth. For artificial sesame flavor, the total VC content was 16,507.9 ppm, and the major VCs were 2-butanone, guaiacol, and 4-vinylguaiacol. Approximately 35.02% of the total VC content of sesame flavor was composed of 2-butanone, guaiacol, and 4-vinylguaiacol, and these constituted more than 1,000 ppm of its total VC content.

• Land and Housing Review
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• v.4 no.1
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• pp.119-124
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• 2013

Generally, the characteristic of nanofiltration membranes were catagorized into charged membrane, sieve effect, interaction between membarnes and target solutes. This study aims to investigate the effect item of heavy metal separation with view of charge nanofiltration membranes. The experiments of nanofiltration were conducted by nanofiltration set-up with operational pressure of 0.24 MPa at $25^{\circ}C$ by using synthetic wastewater containing 0.1mg/L of Cr, Fe, Cu, Zn, As, Sn, Pb. Nanofiltration membranes rejected heavy metals much better than chloride, sulfate and TOC, of which concentration in synthetic wastewater was higher than that of heavy metals. To consider rejection characteristics of various metals by nanofiltration membranes, separation coefficient, which is the molar conductivity ratio of the metal permeation rate to the chloride ion or TOC permeation rate, was introduced. In spite of different materials and different nominal salt rejection of nanofiltration membrane used, the separation coefficients of metals were nearly the same. These phenomena were observed in the relationship between the molar conductivity and the separation coefficient for heavy metals.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.5
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• pp.917-923
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• 2002

Inhibitory mechanism of the anticoagulant polysaccharide purified from Codium fragile was investigated. The anticoagulant compounds (Cf-30-IV-4-ii, CF-30-IV) prolonged the clotting time at both activated partial thrombo-plastin time (aPTT) and thrombin time (TT). The Inhibition factor assay of instrinsic coagulation pathway in the blood showed that the anticoagulant polysaccharide (CF-30-IV-4-ii) inhibited other factors such as Ⅷ, Ⅸ, Ⅵ and Ⅷ of the coagulation cascade, which did not affect the lupus anticoagulant AB activity. In the thrombin inhibition pattern the CF-30-IV-4-ii did not directly influence the fibrine formation mediated by thrombin but af-fected the anticoagulant activity through the activation of antithrombin III. Base on these result, the anticoaglant polysaccharide (CF-30-IV-4-ii) was considered to inhibit serine pretense involved in the blood coagulation cascade through the enhancing antithrombin III activity. The residual effects of anticoagulant activity and antithrombosis were tested with ICR mice. The anticoagulant polysaccharide (CF-30-W) kept its anticoagulant activitv for 6 hrs with 100% survival at a dose of 150 mg/kg in the antithromboisis test. The anticoagulant effect of CF-30-RF in ex vivo was proportional to the concentration of intravenously injected dose up to 100 mg/kg.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.11
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• pp.641-649
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• 2017

In the study, the water quality of the Imjin River basin with pollutants of changing characteristics it was determined through statistical analysis, correlation analysis, principle component and factor analysis, and cluster analysis. Among all analyzed data points, the average water quality concentration at the Sincheon 3 site shows high levels of BOD 13.4 mg/L, COD 19.9 mg/L, T-N 11.145 mg/L, T-P 0.336 mg/L, TOC 14.2 mg/L, indicating that Sincheon basin requires intersive water quality management out of the entire drainage basin. The correlational analysis of comprehensive water quality data shows statistically significant correlation between COD, TOC, BOD, T-N water quality factors, as well as finding of high correlation between organic and nutrients. The principal component analysis show that 2 main components being extracted at 81.221% from the measuring station's entire data, while seasonal data show 3 main components being extracted at 96.241%. Factor analysis of the entire data set and the seasonal data identify BOD, COD, T-N, T-P, TOC as the common factors influencing water quality. The spatial and temporal cluster analysis showed 4 groups and 3 groups, respectively, according to seasonal characteristics and land use. By analysing the water quality factors for the Imjin River basins over an 8 year period, with consideration to the spatial and temporal characteristics, this study will become the fundamental analytic data that will help understand the future changes of water quality in the Imjin River basin.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.30 no.2
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• pp.110-118
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• 2012

This study aimed to research theoretical reviews of streetscape and to analysis the correlation between visual properties and preference of streetscape in Bukchon around Gahoe-dong which is a dense Hanok area. Therefore we analyzed a relation between visual quantities and preference intended Samcheong-dong 35 street, Gahoe-dong 31 street and Gahoe-dong 11 street the representative streetscape in Bukchon. Also we extracted common factors of images of the streetscape in Bukchon and figured out a relation between the visual image evaluation and the preference by regression analysis. The result of the analysis is as follows. First, it was the streetscape of Gahoe-dong 31 street which get more scores than any other streetscape in preference. And we figured out that the higher the ceiling and the visual quantities of planting the more preference. Second, streetscape in Bukchon was explained by 'aesthetics', 'amenity' and 'orderliness' in psychological view. And there was noticeable difference in aesthetics aspect between Gahoe-dong 31 street the highest preferred place and the Samcheong-dong 35 street the lowest preferred place. Third, as a result of regression analysis on preference and factor scores about psychological variables, we figured out that the factor which explains aesthetics effects most on the preference.

• Journal of the Korean earth science society
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• v.38 no.2
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• pp.161-171
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• 2017

Observed ground motions are composed of three factors such as, seismic source, attenuation, and site amplification effect. Among them, the site amplification characteristics should be considered significantly when estimating seismic source and attenuation characteristics with more confidence. The site effect is also necessary when estimating not only seismic hazard in seismic design engineering but also rock mechanical properties. This study uses the method of H/V spectral ratio of observed ground motion between target site and reference site called a reference site method. In addition to using the vertical Fourier spectrum of the reference site, we try out the horizontal Fourier spectrum as a new method in this study. We analyze H/V spectral ratio of six ground motions respectively, observed at four sites close to Yedang Reservoir. We then compare site amplification effects at each site using 3 kinds of seismic energies including S waves, Coda waves energy, and background noise. The results suggest that each site showed similar site amplification patterns in S waves and Coda waves energy. However, the site amplification of background noise shows much different characteristics from those of S waves and Coda wave energy, which suggests that the background noises at each site have their own developing mechanism. Each station shows its own characteristics of specific resonance frequency and site amplification properties in low, high and specific resonance frequency ranges. Comparison of the method used in this study to the others that used different methods can provide us with more information about the dynamic amplification of a site characteristics and site classification.