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Functional Analysis of an Antibiotic Regulatory Gene, afsR2 in S. lividans through DNA microarray System  

Kim, Chang-Young (Department of Biological Engineering, Inha University)
Noh, Jun-Hee (Department of Biological Engineering, Inha University)
Lee, Han-Na (Department of Biological Engineering, Inha University)
Kim, Eung-Soo (Department of Biological Engineering, Inha University)
Publication Information
KSBB Journal / v.24, no.3, 2009 , pp. 259-266 More about this Journal
Abstract
AfsR2 in Streptomyces lividans, a 63-amino acid protein with limited sequence homology to Streptomyces sigma factors, has been known for a global regulatory protein stimulating multiple antibiotic biosynthetic pathways. Although the detailed regulatory mechanism of AfsK-AfsR-AfsR2 system has been well characterized, very little information about the AfsR2-dependent down-stream regulatory genes were characterized. Recently, the null mutant of afsS in S. coelicolor (the identical ortholog of afsR2) has been characterized through DNA microarray system, revealing that afsS deletion regulated several genes involved in antibiotic biosynthesis as well as phosphate-starvation. Through comparative DNA microarray analysis of afsR2-overexpressed S. lividans, here we also identify several afsR2-dependent genes involved in phosphate starvation, morphological differentiation, and antibiotic regulation in S. lividans, confirming that the AfsR2 plays an important pleiotrophic regulatory role in Streptomyces species.
Keywords
afsR2; Streptomyces lividans; DNA-microarray system; sigma factor;
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