• Journal of Food Hygiene and Safety
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• v.34 no.2
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• pp.183-190
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• 2019

Ursolic acid is recognized for various effects such as anti-cancer, antioxidant, and anti-inflammatory activity. In this study, we confirmed the anti-cancer effect of ursolic acid on human melanoma cancer cells, A375SM and A375P. Survival rate of the melanoma cells was confirmed by MTT assay and the proliferation rate was confirmed by wound healing assay. The rate of apoptotic bodies was confirmed by DAPI staining, and apoptosis rate was confirmed by flow cytometry. The induction of apoptosis protein was examined by western blotting according to the concentration of ursolic acid in melanoma cells. The survival and proliferation rates of melanoma cells were decreased according to the treatment concentrations of ursolic acid. DAPI staining showed that chromosomal condensation of melanoma cells was increased with increasing concentrations of ursolic acid, and increased apoptosis rate of melanoma cells by ursolic acid was confirmed by flow cytometry. We also confirmed by western blotting that cleaved-PARP and Bax were increased and Bcl-2 was decreased at $12{\mu}M$ concentration of uricolic acid in melanoma cells. This study was carried out at low concentrations of ursolic acid, 0 to $20{\mu}M$, and analyzed 24 h after treatment. As a result of this study, it is thought that ursolic acid has the anti-cancer effect through the regulation of apoptosis-related proteins in melanoma cells A375SM and A375P.

• Journal of Wetlands Research
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• v.26 no.1
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• pp.62-71
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• 2024

To assess the ecological changes of Korean fir (Abies koreana E. H. Wilson) under climate change conditions, growth and physiological responses were analyzed over a 5-year period in a control group (outdoors) and in a treatment group where the temperature and CO2 levels were elevated to closely resemble RCP 4.5 conditions. The results showed an increasing trend in annual branch length of A.koreana in the climate change treatment group over time. While climate change conditions did not significantly impact the morphological differences of A.koreana leaves, they did influence the biomass of the leaves, suggesting that as climate change progresses, the productivity of A.koreana leaves may decline. On the other hand, the chlorophyll content in A.koreana under climate change conditions was higher in the climate change treatment group, whereas the photosynthesis rate, transpiration rate, water use efficiency and stomatal conductance was higher in the control group. This suggests that an environment with elevated temperature and CO2 could influence an increase in stomatal density, but having a negative impact on photosynthetic reactions. Further research on stomatal density under each environmental treatment will be required to confirm this hypothesis. Additionally, as this study only observed changes in leaf biomass, further empirical research should be considered to understand the changes in biomass of A.koreana under climate change conditions. In conclusion, the environmental adaptability of A.koreana is expected to weaken in the long term under elevated temperatures and CO2.

• Cartoon and Animation Studies
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• s.30
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• pp.245-262
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• 2013

This paper analyzes three feature films that are based on Brothers Grimm's "Snow White": Disney's Snow White and the Seven Dwarfs (1937), Tarsem Singh's Mirror Mirror (2012), and Rupert Sanders' Snow White and the Huntsman (2012). Disney's animation, not the original literature, is the archetype of the later films. Grimm's fairy tail does not include the kiss of Prince Charming that saved Snow White which is, in fact, borrowed from "Sleeping Beauty", nor Snow White's rapport with animals. In Snow White and the Huntsman 's case, the costume of protagonist is similar with Disney's film and some shots are almost identical with Disney's version in terms of composition and angles. Nevertheless, these films show their originality with markedly different visual styles. Mirror Mirror and Snow White and the Huntsman have achieved reasonable success at the box office despite of relatively simple and predictable narratives due to the power of spectacle. While Disney's Snow White displays the model of witch that later becomes prototype of many movies, Mirror Mirror represents the unique magical world, a trompe-l'oell that can only done by director Tarsem, and Snow White and the Huntsman successfully visualizes Freudian concept of 'the uncanny' itself.

• Journal of Wetlands Research
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• v.25 no.3
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• pp.196-204
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• 2023

Eleutherococcus gracilistylus is a designated rare plant by the Korea Forest Service, a deciduous broad leaf shrub native to the Gotjawal region, Jejudo. This study aimed to analyze the growth responses of E. gracilistylus to three environment factors such as light, moisture, and organic matter, and measure its ecological niche breadth. Based on these results, an attempt was made to identify suitable environmental conditions. E. gracilistylus exhibited increased above-ground length, leaf area, and plant leaf weight under intermediate conditions of light availability(L3, 50% of natural light), rather than very high or very low light conditions. Moisture availability and organic matter availability showed variations in growth responses in terms of leaf count and plant leaf weight. Under moisture availability, growth was favorable under or below intermediate conditions (M3, 240ml), while under organic matter availability, growth response was better above intermediate conditions (N3, 12%). Ecological niche breadth showed in the light factor(0.951), the moisture factor(0.977), and the organic matter content one(0.964). These results indicate that the preferred habitat of E. gracilistylus is somewhat shady, slightly dry, and has a lot of nutrients, and that the environmental factor that has the greatest impact on growth is the amount of light, which is considered to be a priority consideration for habitat management in its native area.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.41-41
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• 2022

제주 내 크게 6개 지역에 흩어져 분포하고 있는 곶자왈은 화산활동으로 형성되었으며 제주 산림생물자원의 46%가 분포하여 생물종다양성이 매우 높은 것으로 알려져 있다. 곶자왈은 자갈과 바위로 이루어져 있으며 토양이 적게 발달하여 건조에 직면할 가능성이 크다. 본 연구는 곶자왈에서 서식하는 멸종위기종이자 산림청에서 희귀식물로 지정한 개가시나무(Quercus gilva)와 같은 속이면서 공존하고 있는 종가시나무(Quercus glauca)의 건조 내성에 대한 반응을 알아보고자 실내에서 생리 반응을 연구하였다. 온도(22±2.94℃)와 토양 양분(10%)은 자생지의 값을 기준으로 처리하였으며 수분 처리는 토양을 채운 화분에 물이 화분 밑으로 새어나가기 직전까지 포장용수량 700mL를 공급한 후 27일간 단수하였다. 단수기간에 수분함량의 변화는 토양수분함량측정기(Watch Dog)를 설치하여 데이터를 수집하였다. 항목은 토양수분함량에 따른 광합성률, 증산률, 수분이용효율, 기공전도도, Fv/Fm, Fo, Fm, 엽록소함량을 측정하였다. 토양수분함량은 25, 27일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. 광합성률은 27일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. 증산률은 4, 5, 11, 14, 25, 27일째에 개가시나무가 종가시나무보다 낮았다. 수분이용효율은 13일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. 기공전도도는 3, 9일째에 개가시나무가 종가시나무보다 높았다. Fv/Fm은 22, 27일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. Fo은 3, 14일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. Fm과 엽록소함량은 기간에 개가시나무가 종가시나무보다 높았다. 이와 같은 실험 결과로 보았을 때 개가시나무가 종가시나무보다 건조 내성에 강한 것으로 판단된다.

• The Journal of the Korea Contents Association
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• v.7 no.1
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• pp.199-206
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• 2007

Animation becomes combination of narrative together visual(image) and hearing(sound) information rather than transmits only visual information. The image and sound of such animation are adjusted and controlled in the structure of narrative, and com to receive a meaning by its context. Even if animation begun by equal motive, the meaning which is different in each other with a director is generated. Although Snow White of the Walt Disney Co. and Fleischer's studio was manufactured by motive in fairy tale Snow White, it can look for a large difference in semantic generation of narrative. While Disney's Snow White re-interpreted the original meaning for a director's view of the world and re-made it by animation, Snow White of Fleischer constitutes a work from a meaning of the Snow White original newly completely, is not only weakening the union power of narrative, and but also reflecting the situation at that time.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.46-46
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• 2019

본 연구는 대한민국 안면도 식재의 동백나무 열매를 연차별로 수집하여 크기 범위의 형질특성을 비교하였다. 열매 길이는 24.1~36.0mm가 74.97%를 차지하였으며, 27.1~30.0mm가 23.47%로서 가장 높은 분포 비율을 나타내었다. 연도별 열매 길이는 2015년과 2017년이 각각 27.1~30.0mm, 2016년이 30.1~33.0mm에서 가장 길었다. 열매 너비 (폭)는 27.1~39.0mm가 74.36%를 차지하였으며, 33.1~36.0mm가 22.90%로서 가장 높은 분포 비율을 나타내었다. 연도별 열매 너비는 2015년과 2016년이 각각 33.1~36.0mm, 2017년이 30.1~33.0mm에서 가장 넓었다. 열매 장폭비는 0.81~1.00%가 64.77%를 차지하였으며, 0.81~0.90%가 33.27%로서 가장 높은 분포 비율을 나타내었다. 연도별 열매 장폭비는 2015년, 2016년, 2017년 모두 0.81~0.90%에서 가장 높았다 따라서 열매는 길이보다 너비 (폭)가 약간 넓은 둥근 모양을 나타내었다. 열매 무게는 5.1~25.0g이 78.31%를 차지하였으며, 15.1~20.0g이 25.37%로서 가장 높은 분포 비율을 나타내었다. 연도별 열매 무게는 2015년, 2016년, 2017년 모두 15.1~20.0g에서 가장 무거웠다. 그리고 열매 형질 사이의 상관관계를 보면 연차별 모두 열매의 너비 (폭)는 길이, 무게와 정의 유의한 상관이 있었다.

• Journal of Environmental Science International
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• v.28 no.4
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• pp.435-439
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• 2019

This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of $9,889{\pm}3{\mu}g/L$ to $12,909{\pm}2{\mu}g/L$ at 45 days and $29,256{\pm}4{\mu}g/L$ at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to $83,751{\pm}2{\mu}g/L$ after 45 days from its initial value of $161,787{\pm}1{\mu}g/L$ and to $79,055{\pm}7{\mu}g/L$ at 90 days.

• Journal of Wetlands Research
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• v.25 no.3
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• pp.205-212
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• 2023

This study was attempted to find out how the ecological niche and interspecies relationship of Quercus aliena and Q. serrata, which are the main constituents of potential natural vegetation along the riverside of mountains in Korea, under climate change conditions. To this end, soil moisture and soil nutrients were treated with 4 grad ients under climate change conditions with elevated CO₂ and temperature, plants we re harvested at the end of the growing season, growth responses of traits were measured, ecological niche breadth and overlap were calculated, and it was compared with that of the control group(ambient condition). In addition, the relationship between the two species was analyzed by principal component analysis using trait values. As a result, the ecological niche breadth of Q. aliena was wider than that of Q. serrata under the moisture environment conditions under climate change. Under nutrient conditions, the ecological niche of the two species were similar. In addition, the ecological overlap for soil moisture of Q. aliena and Q. serrata was wider than the soil nutrient gradient under climate change. The species with traits in which the increase in ecological niche breadth due to climate change occurred more than the decrease was Q. aliena in both water and nutrient gradients. And in the responses of the population level, due to climate change, the adaptability of Q. aliena was higher than that of Q. serrata under the soil moisture condition, but the two species were similar under the nutrient condition. These results mean that the competition between the two species occurs more severely in the water environment under climate change conditions, and at that time, Q. aliena has higher adaptability than Q. serrata.

• Bulletin of the Korean Chemical Society
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• v.18 no.1
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• pp.103-105
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• 1997