Changes of Free Amino acid in PDB Medium by Lentinula edodes (Sanjo 701ho) Cultivation period

This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of $9,889{\pm}3{\mu}g/L$ to $12,909{\pm}2{\mu}g/L$ at 45 days and $29,256{\pm}4{\mu}g/L$ at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to $83,751{\pm}2{\mu}g/L$ after 45 days from its initial value of $161,787{\pm}1{\mu}g/L$ and to $79,055{\pm}7{\mu}g/L$ at 90 days.

표고버섯 균사체 배양기간에 따른 PDB 배지 내 유리 아미노산 변화