• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1975.12a
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• pp.180.4-181
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• 1975

토양에서 분리한 한주인 Streptomyces sp. No.14를 당질인 xylose 및 xylan과 천연배지로 wheat Bran 및 Corn Cob를 함유한 영양배지에 배양한 결과 균체로부터 강력한 glucose isomerase를 생산하였다. 따라서 이 균주의 배양 및 효소적 특성을 몇 가지 조사한 결과는 다음과 같다.(중략)

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2002.10a
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• pp.92-92
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• 2002

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.10a
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• pp.207.3-207
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• 1978

TI(trypsin inhibitor) 생성에 관한 여러가지 조건을 검토한 결과 gluose 1%, NaNO$_3$ 0.2%의 조성을 가진 pH 7.0의 배지에서 3 일간 배양하는것이 물질생성에 가장 좋았다.

• Korean Journal of Plant Tissue Culture
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• v.28 no.3
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• pp.173-173
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• 2001

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1977.10a
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• pp.196.1-196
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• 1977

전보에서 보고한 바 있는 Cellulomonas속 한 균주와 분리선정한 보조균을 CM-Cellulose로 기회로 하여 혼합배양하였을 때 각각 단독으로 배양하였을 때 보다 균주증식이 아주 양호하였음을 알았으며 분리 보조균에 대한 균학적 성질을 조사한 결과 Sporocytophaga속의 성질과 거의 일치하였다.(중략)

• Korean Journal of Plant Tissue Culture
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• v.22 no.4
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• pp.213-213
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• 1995

• Korean Journal of Animal Reproduction
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• v.24 no.3
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• pp.289-297
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• 2000

This study was conducted to investigate whether various protein sources and co-culture affect in vitro development of porcine zygotes derived from In vitro maturation/fertilization (IVM/IVF). These results obtained in these experiments are summarized as follows 1. When porcine oocytes matured and fertilized In vitro were cultured in NCSU 23 medium supplemented with various BSA concentrations (0.4, 0.8 and 3.2%), In vitro developmental rates of porcine zygotes to blastocyst stage were 22.9, 18.4 and 14.6%, respectively. High concentration of BSA (3.2%) showed a smaller nuclei number (36.1$\pm$11.8) of blastocysts than 0.4 and 0.8% BSA groups (53.2$\pm$27.4 and 61.2$\pm$22.5, respectively) (P<0.05). This result indicates that high concentration of BSA is detrimental on preimplantation development of IVF-derived porcine embryos. 2. No differences were detected in the developmental rate and mean nuclei number of porcine embryos between 10 and 20% FBS concentrations in culture medium. 3. IVF-derived porcine embryos co-cultured with mouse or porcine embryonic fibroblast cells showed a lower development to the blastocyst stage than those without co-culture system. Consequently, the present study suggests that high concentration of BSA as a protein source in culture medium suppresses development potential of porcine embryos produced In vitro. In addition, co-culture with somatic cells is not effective on in vitro development of IVF-derived porcine embryos to blastocyst stage.

• KSBB Journal
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• v.11 no.4
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• pp.453-461
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• 1996

We compared the effects of two different systems of collagen matrix protein application on the survival and the biological functions of cultured primary hepatocytes. The rat liver primary hepatocytes were grown for approximately 40 days in vitro either on single collagen gel or between collagen sandwich gels. The morphological changes were observed for this culture period. While the hepatocytes grown on single gel began to die around at 7 days of culture, the cells grown between collagen gels still maintained their viability and began to die after 15 days. As markers for liver hepatic functions, we determined the biochemical activities of hepatocytes such as the secretions of albumin, fibronectin, fibrinogen, urea, and the reduction of secreted ammonia. We found that the rat hepatocytes cultured between collagen gels maintained fairly good biochemical functions than the hepatocytes cultured on single gel did. Therefore, the application of an extracellular matrix protein, collagen, in sandwich form was confirmed as a better choice for maintaining the functional hepatocytes culture for long term in vitro.

• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.335-339
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• 2001

Grapevine leafroll-associated 3 closterovirus (GLRaV-3) is phloem limited virus, and one of the most severe viral diseases found in Korea. However, nonhomogenous distribution and low concentration and seasonal variations of GLRaV-3 in grapevines remain as main problems which prevent the introduction and molecular biology or serology experiments. Virus-infected plantlet in vitro was obtained from node tissue cuttings, which was GLRaV-3 infected 'kyoho' vines. The amount of purified virus was highest in vitro plantlet. Moreover, viruses seem to be relatively homogeneously distributed in all organs including leaf, stem and callus derived from in vitro plantlets. RT-PCR detection using in vitro plantlet tissue as template was most effective. When comparing ELISA to RT-PCR, RT-PCR detection was 1,000 times as effective as ELISA. These results can be explained by improved quality such as tenderness or less tannins in plantlet in vitro. In conclusion, until infected herbaceous host will be available, tissue culture can be usefully adopted as a technique for a good source of GLRaV-3 closterovirus for further studies.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.110-114
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• 2010

Highest increase of biomass was observed when tissue-cultured potato (Solanum tuberosum L. cv. Chubaek) shoots were cultured in a liquid medium containing 1/3 MS solution in a 18 L bioreactor, as compared to 1/4 and 1/2 MS solution. The medium containing 1/4 MS solution showed higher increase of shoot biomass than one containing 1/2 MS solution. Potato microtubers were formed when the medium was exchanged with the medium for microtuber formation and incubated under dark condition. The microtubers were observed first at some axillary buds one week after incubation under dark condition and then at most of the axillary buds by the end of 3 weeks. The 1.5 MS liquid medium and $20^{\circ}C$ were optimal conditions. By the end of 6 weeks, more 1,000 microtubers were formed in the 18 L bioreactor. Then, greened microtubers were harvested after one week culture under light condition.