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http://dx.doi.org/10.5010/JPB.2010.37.1.110

Mass production of potato microtubers by bioreactor culture  

Kim, Jae-Whune (Microplants)
Choi, Eun-Gyung (Microplants)
Oh, Seung-Cheol (Microplants)
Joo, Sun-Ah (Microplants)
You, Dong-Min (School of Life Sciences and Biotechnology, Korea University)
Kim, Soon-Kap (School of Life Sciences and Biotechnology, Korea University)
Kim, Jeong-Kook (School of Life Sciences and Biotechnology, Korea University)
Publication Information
Journal of Plant Biotechnology / v.37, no.1, 2010 , pp. 110-114 More about this Journal
Abstract
Highest increase of biomass was observed when tissue-cultured potato (Solanum tuberosum L. cv. Chubaek) shoots were cultured in a liquid medium containing 1/3 MS solution in a 18 L bioreactor, as compared to 1/4 and 1/2 MS solution. The medium containing 1/4 MS solution showed higher increase of shoot biomass than one containing 1/2 MS solution. Potato microtubers were formed when the medium was exchanged with the medium for microtuber formation and incubated under dark condition. The microtubers were observed first at some axillary buds one week after incubation under dark condition and then at most of the axillary buds by the end of 3 weeks. The 1.5 MS liquid medium and $20^{\circ}C$ were optimal conditions. By the end of 6 weeks, more 1,000 microtubers were formed in the 18 L bioreactor. Then, greened microtubers were harvested after one week culture under light condition.
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Times Cited By KSCI : 2  (Citation Analysis)
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