• KSBB Journal
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• v.14 no.4
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• pp.445-451
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• 1999

Glutathione production was carried out using mixed cells of E. coli TG1/pDG7 $\alpha$ and bakers yeast in an Aerated Slurry Bioreactor. Glutathione-producing enzymes were stable for 34 hours, yielding 4.6 mM glutathione in suspension reaction. Glutahione production with high density mixed cells was studied as a function of flow rate in an Aereated Slurry Bioreactor. Glutathione concentration was higher than that in suspension reaction for 32 hours at the substrate feeding rate of 5.2 mL/hr with cell recycle in continuous Aerated Slurry Bioreactor. It was for 42 hours at 2.6 mL/hr and 22 hours at 5.2 mL/hr without cell recycle. Glutahione productivity was 25.7 mg/g wet $cell{\cdot}hr$ at the substrate feeding rate of 10.4 mL/hr with cell recycle, but 5.28 mg/g wet $cell{\cdot}hr$ at 5.2 mL/hr and 1.65 mg/g wet $cell{\cdot}hr$ at 2.6 mL/hr without cell recycle. Effective production time increased from 25 to 45 hours, by using a surfactant, tween 80. As a purfing gas, nitrogen was tested instead of air to avoid a possible oxidizing effect on glutathione-producing enzymes, resulting in the increase of effective production time to 40 hours.

• Journal of the Korean Institute of Gas
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• v.5 no.2 s.14
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• pp.22-29
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• 2001

The number of gas containers and the period of exchanging gas containers are vsy important in designing liquefied petroleum gas(LPG) supply system for small capacity domain. And also the evaluation of remaining LPG in containers to be exchanged is very useful information in commerce. However seldon has been studied on calculating method about those with respect to gas consumption pattern. In this study, a simulation method was developed to estimate the evaporation capacity of LPG container, the mass gas flow rate from LPG container, the temperature and vapor pressure of LPG, and the remained LPG at containers to be exchange by using LPG property equations, mass balance equation, and heat balance equation. The simulation results were correlated well with experimental data. The overall heat transfer coefficient from air to LPG is approximately $9{\~}13 kcal/m^2{\cdot}hr{\cdot}^{\circ}C$ and does not strongly affect on the evaporation capacity of LPG container. The mass gas flow rate from LPG container is constant when the vapor pressure of LPG is within pressure regulator's control range. While, out of range, it suddenly reduce to a evaporation rate which is balanced with heat transfer from air. The evaporation capacity of LPG container increased with surrounding temperature and the composition of propane, and decreased drastically with continuous gas consumption. The number of gas containers divided the number of houses using gas supply system was reduced by using automatic gas feeding device.

• Journal of Korean Tunnelling and Underground Space Association
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• v.20 no.6
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• pp.1091-1104
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• 2018

Wear prediction of TBM disc cutters is a very important issue during design as well as construction stages for hard rock TBMs as the cutter head intervention is directly related to the time and cost of tunneling. For that, some methods such as NTNU, CSM and Gehring models were used to predict disc cutter wear and intervention interval. There are however some problems to be addressed in these models in terms of accuracy and time for testing, so that a NAT (New Abrasion Tester) model has been developed in order to achieve simplicity and reliability together at the same time (Farrokh and Kim, 2018). On the basis, the proposed NAT model has been applied to ${\bigcirc}{\bigcirc}$ project in Korea. A comparative study was performed to compare with the conventional methods and as a result the NAT model showed a very good agreement with actual cutter life. The NAT model will be further applied to other projects to establish credibility.

• International Journal of Highway Engineering
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• v.8 no.2 s.28
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• pp.1-12
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• 2006

An existing Steel pipe, Cast iron pipe and Concrete pipe is can not escaped from aging, specially Metal tube is causing many problems that the quality of water worse is concerned about many rust and mike efficient use of preservation of water. The use of Glassfiber Reinforced Plastic Pipe(GRP PIPE) should be one of the possible scheme to get over these problems. The GRP PIPE has an excellent resistance power and the life is lasting from 50 to 100 years roughly. It's to be useful as a result of high durability and a good construction work also it is a light weight therefore can be expected to short the time of construction and man power. In this research, to executed the small-scaled model test, in-situ model test using CLSM of in-situ soil and to evaluated the stress - strain of the pipe also try to estimated how useful is. From the model test in laboratory, the vertical and horizontal deformation of the GRP PIPE measured in six instance using 200mm and 300mm in diameters. The value of experimentation, theory, analysis got the same results of the test, but the vertical and horizontal deformation gauged in small and the earth pressure was almost zero using CLSM of in-situ soil..