• Journal of Veterinary Clinics
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• v.32 no.6
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• pp.511-513
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• 2015

A 12-year-old spayed Pomeranian was presented with a purulent, erythematous mass. The dog also suffered from concurrent hyperadrenocorticism and diabetes mellitus. Sporothrix schenckii was isolated through the fungal culture of the mass. Surgical ablation of the mass was performed for the removal of continuous inflammation and fungal infection of deep origin. After histological examination of the mass, severe multifocal pyogranulomatous dermatitis and panniculitis were diagnosed. This is the first confirmed case of pyogranulomatous dermatitis and panniculitis due to Sporothrix schenckii infection in a dog with concurrent hyperadrenocorticism and diabetes mellitus. In addition this is the first case report of sporotrichosis in South Korea.

• Research in Plant Disease
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• v.7 no.3
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• pp.134-139
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• 2001

Effects of tomato-juice and KH$_2$PO$_4$ as exogenous nutrients on the infection of Botrytis cinerea LVF12 and pathogenicity to tomato were investigated. B. cinerea LVF12, which was previously reported as a casual agent of the gray mold rot of perilla, was used for pathogenesis on tomato leaves. No infection was induced, and no lesion developed on tomato leaves by the conidial suspension of LVF12 when the inoculum was prepared in sterilized water. However, when the conidial suspensions of LVF12 added with various concentrations and conditions of tomato-juice were inoculated on whole tomato plants, the disease was induced readily, Among them, 20% tomato juice with 0.1M KH$_2$PO$_4$ appeared to be the most suitable nutrient to promote high disease incidence on tomato. For the pathogenicity test according to the growing stage of tomato, the mature leaves were more susceptible than seedlings. Symptoms on the infected plants were initial small gray spots at the inoculated area. Later the whole leaves, petioles and stems became gray and eventually fell off, Under high humidity conditions, the diseased leaves and stems were covered with gray hyphae and conidia. All symptoms of infected plants were identical to those in the field conditions.

• Research in Plant Disease
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• v.27 no.4
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• pp.172-179
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• 2021

This study aimed to assess the disease incidence and distribution of toxigenic in Korean triticale. The pathogen of triticale that cause Fusarium head blight were isolated from five different triticale cultivars that cultivated in Suwon Korea at 2021 year. The 72 candidate were classified as a Fusarium asiaticum by morphology analysis and by ITS1, TEF-1α gene sequence analysis. And the results of pathogenicity with 72 isolates on seedling triticale, 71 isolates were showed disease symptom. Also, seven out of 71 Fusarium isolates were inoculated on the wheat, to test the pathogenicity on the different host. The results showed more low pathogenicity on the wheat than triticale. The results of analysis of toxin type with 72 isolates, 64.6% isolates were produced nivalenol type toxin and other 4.6% and 30.8% isolates were produce 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol, respectively. To select fungicide for control, the 72 Fusarium isolates were cultivated on the media that containing four kinds fungicide. The captan, hexaconazole, and difenoconazole·propiconazole treated Fusarium isolates were not showed resistance response against each fungicide. However, six isolates out of 72 isolates, showed resistance response to fludioxonil. This study is first report that F. asiaticum causes Fusarium head blight disease of triticale in Korea.

• Journal of Veterinary Clinics
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• v.32 no.3
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• pp.259-262
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• 2015

A canine patient exhibited partial anorexia and sudden respiratory distress. Diagnostic imaging and cytology of tracheal-lavage fluids revealed fungal pneumonia. Molecular identification and phylogenetic analysis detected Candida catenulata. Treatment with oral itraconazole for 3 weeks was effective. This is the first report of C. catenulata infection in a dog.

• Journal of Ginseng Research
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• v.30 no.4
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• pp.206-211
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• 2006

To investigate mycorrhizal infection by arbuscular mycorrhizal fungi(AMF), samples of fine lateral roots were taken from the wild ginseng(Panax ginseng C.A. Meyer) naturally growing at various locations in Korea. Mycorrhiazal infections were studied by cleaning the root samples and staining fungal hyphae with frypan blue. Wild ginsengs for this study were graded by an appraisal committee consisting of 12 experts of Korea Mountain Ginseng Association. Following five quality groups were recognized: Heaven group(pure natural), Earth group (from seeding of wild ginseng), Man group(from seeding or seedlings of wild ginseng with slight environmental modification), unmarketable, and imported wild ginseng. Morphology of AMF was typical Paris-type which shows intracellular hyphal coils with rare vesicles and lack of arbuscules. Average infection rate of individual wild ginsengs was 58.3% and showed no differences among five quality groups. When portions of fine roots were quantified for mycorrhizal infection, 18.7% of the total length of the primary and secondary roots were infected by AMF. Wild ginsengs from Gyeonggi Province(84.2%), and from mountains lower than 1,200 meters above sea level(about 70%) showed higher infection rate, while the ginseng from Gyeongbuk Province(27.8%) had lower rate. Wild ginsengs at older age showed lower infection rates.

• Journal of Applied Biological Chemistry
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• v.61 no.1
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• pp.69-73
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• 2018

Oak mushroom (Lentinus edodes) is cultivated by using oak logs and sawdust medium. Green mold (Trichoderma) infection on these media severely suppresses the growth of oak mushroom. Usages of antibiotics and chemicals are not generally allowed to control of green mold since the mushroom is a fresh food. Tolaasin and its analogues, peptide toxins secreted by Pseudomonas tolaasii, have the antifungal activity and they have been successful to control the green mold disease. When the green mold, Trichoderma harzianum H1, was cultured in the presence of these toxins, the growth of fungus was effectively suppressed. In sawdust media, when the bacterial culture supernatants were sprayed on the aerial hyphae of green molds, the fungal growth was completely suppressed. Particularly, the antifungal activity was greatly increased by the combined culture extracts of P. tolaasii 6264 and HK11 strains. Therefore, these bacterial strains and their peptide toxins were able to suppress the growth of green molds and these can be good candidates to prevent from Trichoderma disease in oak mushroom cultivation.

• The Korean Journal of Mycology
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• v.33 no.2
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• pp.89-91
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• 2005

In April of 2003 to 2004, the gray mold disease caused by Botrytis cinerea on Hypericum ascyron was occurred in the exhibition field of Gyeongsangnam-do Agricultural Research and Extension Services, at Hamyang-gun in Korea. The disease symptoms were started with water-soaking lesions in stem and the infected plants became withered, discolored and died. The conidia and mycelia of the pathogen appeared on stems of infected plants. The conidia were 1-celled and mostly ellipsoid or ovoid in shape and their color was light gray. The size of conidia was $4{\sim}16\;{\times}\;3{\sim}8\;{\mu}m$ and that of conidiophores was $12{\sim}26\;{\mu}m$ respectively. The pathogen formed sclerotia abundantly on potato-dextrose agar. The optimum temperature for sclerotial formation was $20^{\circ}C$. Pathogenicity of the causal organism was proved according to Koch's postulate. The causal organism was identified as Botrytis cinerea based on mycological characteristics. This is the first report on gray mold of H. ascyron caused by B. cinerea in Korea.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.465-469
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• 2016

An entomopathogenic fungus was isolated from the two-spotted spider mite (Tetranychus urticae) in a rearing house, and identified as Neozygites floridana (Entomophthorales: Neozygitaceae). A high infection rate induced by N. floridana could increase the price of the natural enemy. The body color of mites infected by this fungus changed to red or orange and swelling occurred. Fungal conidia were discharged into the webbing produced by the spider mites, making it relatively easy to infect the mites. Primary conidia were pear shaped and capilliconidia almond shaped. The fungus could not be cultured on solid media (PDA, SDAY, or EYSDA), but could possibly be cultured in liquid media (Grace's insect tissue culture medium + 5% fetal bovine serum). Kidney beans were supplied as food for T. urticae; the mite infection rate in a kidney bean canopy was about 36.1%. The density of infected mites was higher on the underside than on the upper side of leaves. Based on the results of this survey, we need to identify methods of fungal control for natural enemy production and biological control agents for T. urticae for effective crop management.

• Journal of Korean Society of Archives and Records Management
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• v.16 no.2
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• pp.173-183
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• 2016

Fungi are a major cause of cultural and biological degradation in museum storages and archives libraries. In addition, fungi produce toxins and can cause skin allergies. In particular, fungi coming into contact with the skin through the hands of administrators dealing with cultural properties, archives, and books can lead to secondary infections. To prevent this, applying hand sanitizer, which is readily available in the market, is recommended. However, such hand sanitizer can effectively disinfect against bacteria but not fungi. As such, researching for an alternative is required. In this study, we developed a hand sanitizer composed of active ingredients - geranial, cineol, menthol, and Chamaecyparis obtusa - and performed a disinfection test, which targets 10 kinds of species (Alternaria citrimacularis, Alternaria consortialis, Aspergillus niger, Aspergillus versicolor, Cladosporium sphaerospermum, Coniothyrium aleurites, Penicillium brevicompactum, Penicillium corylophilum, Penicillium paneum, and Penicillium polonicum), of fungi investigated in museums and libraries. It was then confirmed to exhibit excellent disinfecting effects. Thus, this hand sanitizer is expected to prevent skin diseases and secondary infections in administrators dealing with cultural properties, archives, and books.

• Research in Plant Disease
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• v.12 no.2
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• pp.115-118
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• 2006

In February of 2000-2001, the gray mold disease occurred on nephrolepis (Nephrolepis sp.) grown in a flower nursery farm in Suwen, Korea. Typical symptoms were water-soaked brown or blackish lesions on terminal leaf blades. Severely infected leaves were entirely blighted with grayish fungal mycelia formed on the surface. Conidia of the fungus in mass were hyaline or gray, 1-celled, mostly ellipsoid or ovoid and $13.5{\sim}16.9{\times}6.8{\sim}9.2{\mu}m$ in size. Conidiophores were formed on PDA with $8.7{\sim}11.1{\mu}m$ in width. The sclerotia were readily formed within 2 or 3 days on PDA. In addition, the Biolog database gave the causal fungus a high similarity to Botrytis cinerea (78%) with a match probability of 100%. Pathogenicity of the causal organism was proved according to Koch's postulate. The causal organism was identified as Eotrytis cinerea based on its mycological characteristics and utilization of carbon sources with Biolog system as supporting data. This is the first report of gray mold of nephrolepis caused by Botrytis cinerea in Korea.