• Title/Summary/Keyword: 곡선 프로브

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이중 주파수의 위상변화에 따른 자기바이어스를 이용한 플라즈마 변수 측정

  • Park, Il-Seo;Lee, Hyo-Chang;Kim, Yu-Sin;Kim, Dong-Hwan;Jeong, Jin-Uk
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.584-584
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    • 2013
  • 부유 고조화파 진단법을 이용할 경우에 직렬 커패시터가 달린 프로브에 정현파를 인가하게 되면 자기바이어스가 생긴다. 이런 자기바이어스에 의한 플라즈마 변수 측정에 대한 연구가 진행되어 왔다. 본 연구에서는 아르곤 유도 결합 플라즈마에서 실험을 진행하였으며 서로가 정수 배인 두 개의 주파수를 이용하였다. 이 두 개의 주파수를 평판형 프로브에 인가하여 각각 주파수의 위상 차이에 따라 자기바이어스를 측정하였다. 이 측정값은 이론적으로 구한 위상 차이에 따른 정현파 곡선과 잘 일치하였다. 또한 측정된 자기바이어스를 이용하여 플라즈마 변수를 계산하였으며 이는 랑뮤어 프로브로 측정한 결과와 비슷한 경향성이 나타남을 확인 하였다.

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Soil Water Characteristic Curve Using Volumetric Pressure Plate Extractor Incorporated with TDR System (TDR 측정시스템이 도입된 압력판 추출 시험기를 이용한 흙-함수특성곡선 연구)

  • Jung, Young-Seok;Sa, Hee-Dong;Kang, Seonghun;Oh, Se-Boong;Lee, Jong-Sub
    • Journal of the Korean Geotechnical Society
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    • v.31 no.8
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    • pp.17-28
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    • 2015
  • The purpose of this study is to measure the volumetric water content of unsaturated soils during drying and wetting process by using volumetric pressure plate extractor (VPPE) incorporated with time domain reflectometry (TDR). The VPPE consists of a pressure cell, a pressure regulator, a burette system and a TDR probe. Two samples with different initial void ratios were prepared in the pressure cell, and the air pressure at the range of 0.1 kPa - 50 kPa was applied to adjust the matric suction by the pressure regulator. The burette system was used to measure the volumetric water content change of the sample according to the matric suction. In addition, the TDR probe, installed in the cell, was used to evaluate the dielectric constant from the reflected signal of the electromagnetic wave at the probe. The volumetric water content of specimen was estimated by the empirical equation between the volumetric water content and dielectric constant, which was calibrated with the Jumunjin sand. The test results show that the volumetric water content calculated by TDR probe is strongly correlated to the measured value by burette system. The hysteresis occurs during drying and wetting process. Furthermore, the degree of hysteresis reduces in the repeated process. This study suggests that TDR may be effectively used to evaluate the water content soil for the determination of water characteristic curve of unsaturated soils.

Reconstruction of 3D Ultrasound Image from Freehand Scanned Frames Using Lateral Correlation Functions (측면거리 상관함수를 이용한 수동주사 초음파 영상 프레임들로부터의 3차원 영상 재구성)

  • 이준호;김남철;김상현
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.8B
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    • pp.1152-1160
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    • 2001
  • 본 논문에서는 수동주사로 얻은 2차원 연속 프레임으로 3차원 초음파 영상 재구성을 위한 연속 프레임의 프레임간 거리 추정 방법을 제안하였다. 수동주사로 얻은 연속 프레임은 프레임간 거리가 불균일하기 때문에 이를 그대로 3차원 영상으로 재구성하면 실제 인체 장기의 형태와 다른 영상을 얻게 된다. 제안한 알고리듬에서는 연속 프레임의 프레임간 거리를 추정하기 위하여 매 프레임의 블록별 측면거리 상관함수를 얻고, 측면거리 상관함수들이 프로브의 진행축과 초음파 센서의 배열축이 이루는 평면상에서 등방성을 가진다는 가정 하에 인접 프레임 내의 각 블록간의 프레임간 거리를 추정하였다. 인접 프레임간 추정거리는 프레임 내에서 블록단위로 추정된 프레임간 거리를 평균하여 얻었다. 실험 결과, 제안한 알고리듬의 프레임간 추정거리 곡선은 기준 진행거리 상관함수를 이용한 방법의 추정거리 곡선에 비해서 실제 프레임간 거리 곡선에 가까웠고, SNR 비교에서 제안한 방법이 기존의 방법에 비해 좋은 결과를 보였다. 그리고, 기존의 알고리듬에 비해 제안한 알고리듬으로 재구성한 3차원 영상이 원영상에 더 흡사한 것을 볼 수 있었다.

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Development of Real-time PCR Assays for Detection of Dirofilaria immitis from Infected Dog Blood (심장사상충에 감염된 개의 혈액에서 심장사상충 유전자를 검출할 수 있는 실시간 중합효소연쇄반응 기법 개발)

  • Oh, In Young;Kim, Kyung Tae;Jun, Jin Hyun;Shin, Jae-Ho;Sung, Ho Joong
    • Korean Journal of Clinical Laboratory Science
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    • v.48 no.2
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    • pp.88-93
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    • 2016
  • Dirofilaria immitis is a filarial nematode parasite that causes cardiopulmonary dirofilariasis in dogs. The purpose of this study was the development of real-time PCR assays for efficient detection of D. immitis. The D. immitis-specific primers confirmed in our previous study and a newly designed TaqMan probe were used for quantitative diagnostics. First, SYBR Green real-time PCR was performed using the specific primers and serially diluted genomic DNA or plasmid DNA, and melting curve analyses were performed after amplification. The melting curve showed one specific peak in each of the genomic and plasmid DNA reactions, suggesting that the primers specifically amplify the D. immitis cytochrome c oxidase subunit I gene. Comparison of SYBR Green and TaqMan real-time PCR using serially diluted plasmid DNA showed higher efficiency and specificity with TaqMan real-time PCR. The real-time PCR assays developed in this study will provide improved diagnostic methods to overcome the limitations of conventional diagnostic tools and facilitate more rapid and accurate diagnoses.

A Study on the Reflectivity according to the Material of Biopsy Gun Needle Used in Ultrasound Biopsy (초음파 조직검사에 사용되는 Biopsy Gun Needle의 재질에 따른 반사율 연구)

  • Hoon Kim;Cheong-Hwan Lim
    • Journal of radiological science and technology
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    • v.47 no.2
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    • pp.97-105
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    • 2024
  • The examination needle used in ultrasound biopsy is a medical device used to determine whether there is an abnormality in the tissue. Typically, stainless steel is the standard material used for such needles; however, this study wanted to identify a material that could better enhance sound compared to traditional stainless steel. In this study, six types of needle materials available with the biopsy gun were inserted into pork and ultrasound images according to the curved probe and linear probe were evaluated using ultrasound equipment. The findings revealed significant improvements in ultrasound acoustic enhancement with alternative materials compared to stainless steel (p<0.05). The results regarding the depth of each ultrasound image using the curved probe showed that tungsten and brass had high sound enhancement(p<0.05), while with the linear probe, sound enhancement was high in brass, pla, aluminum, and copper(p<0.05). Due to these results, the previously used stainless needle showed lower ultrasound acoustic enhancement than the five types of materials being compared. Consequently, the outcomes of this study provide valuable insights for the development of new needle technologies aimed at minimizing patient risks and improving diagnostic accuracy.

Application of a Peptide Nucleic Acid-Based Asymmetric Real-Time PCR Method for Rapid Detection of Vibrio cholerae (비브리오 콜레라 신속 검출을 위한 펩티드 핵산 기반 비대칭 real-time PCR 방법의 적용)

  • Kang, Mingyeong;Lee, Taek-Kyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.12
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    • pp.117-124
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    • 2019
  • Vibrio cholerae is a very important pathogenic bacterium that has to be monitored in seafood and ships' ballast water. Various methods have been developed to identify this bacterium, yet these methods are time-consuming and have limitations for their sensitivity to detect contamination. The purpose of the present study was to develop a robust and reliable method for identifying V. cholerae. Peptide nucleic acid (PNA) probes were developed to use for PNA-based asymmetrical real-time PCR techniques. The toxigenic Cholera enterotoxin subunit B (ctxB) gene was selected as a target for detecting V. cholerae and the gene was synthesized as a positive template for conventional and real-time PCR. Real-time PCR primers and PNA probes were designed and standard curves were produced for the quantitative analysis. The selected PNA probes reacted specifically to V. cholerae without any ambiguity, even among closely related species, and the detection limit was 0.1 cfu/100 mL. Taken together, the PNA probes and asymmetrical qPCR methods developed in this present study could contribute to the rapid, accurate monitoring of V. cholerae in marine environments, and as well as in seafood and ships' ballast waters.

An Experimental Study of Mutual Relation between Wake and Boundary Layer of a Flat Plate; Mean Velocity Field (평판 경계층과 후류와의 상호관계에 관한 연구; 평균속도장)

  • Kim, Dong-Ha;Chang, Jo-Won
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.32 no.10
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    • pp.1-11
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    • 2004
  • An experimental study was carried out to investigate the influence of flow conditions of a boundary layer on the near-wake of a flat plate. Various attaching positions of tripping wires were selected to change flow conditions on a boundary layer. Laminar, transitional, and turbulent boundary layer conditions at 0.98C from the leading edge are imposed to investigate the evolution of symmetric and asymmetric wake. An x-type hot-wire probe(55P61) is employed to measure at 8 stations of the near-wake region. Measured mean velocity distributions are presented in terms of similarity parameter. It is found that the symmetric wake collapses well to the universal profile in the central part of the wake. However, the universal profile is not suitable in describing an asymmetric wake.

Development of Real-time Quantitative PCR Assay based on SYBR Green I and TaqMan Probe for Detection of Apple Viruses (사과 바이러스 검정을 위한 SYBR Green I 및 TaqMan probe 기반의 real-time PCR 검사법 개발)

  • Heo, Seong;Chung, Yong Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.496-507
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    • 2020
  • Virus infections of apples result in lowered commercial qualities such as low sugar content, weakened tree vigor, and malformed fruits. An effective way to control viruses is to produce virus-free plants based on the development of an accurate and sensitive diagnostic method. In this study, real-time PCR assays based on SYBR Green I and TaqMan probes were developed for detecting ASGV, ASPV, and ApMV viruses. These methods can detect and quantify 103 to 1011 RNA copies/μL of each virus separately. Compared with methods with two different dyes, the SYBR Green I-based method was efficient for virus detection as well as for assay using the TaqMan probe. Field tests demonstrated that real-time PCR methods developed in this study were applicable to high-throughput diagnoses for virus research and plant quarantine.

Degradation Evaluation of Mechanical Properties for 12Cr Ferrite Heat Resisting Steel by Reversible Permeability (가역투자율에 의한 12Cr 페라이트 내열강의 역학적 물성의 열화평가)

  • Ryu, Kwon-Sang;Kim, Min-Gi;Nahm, Seung-Hoon;Kim, Chung-Seok
    • Journal of the Korean Society for Nondestructive Testing
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    • v.30 no.5
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    • pp.464-470
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    • 2010
  • The integrity of the industrial equipment in use under high temperature and high pressure must be assessed by regularly measuring the degraded mechanical properties during service time. In order to nondestructively monitor the degraded mechanical properties of industrial equipment, a measuring method of the reversible permeability(RP) using surface type probe is presented. The method for measuring the RP is based on that RP is the differential value of hysteresis loop. The RP is exactly the foundation hatmonics induced in a detecting coil measured by lock-in amplifier tuned to a frequency of the alternating perturbing magnetic field. The peak of RP is measured around the coercive force. Steel material used in this work was 12Cr ferritic heat resisting steel. The eleven kinds of samples aged during different times under same temperature ($700^{\circ}C$) were prepared. Peak interval of reversible permeability(PIRP), Vickers hardness, and tensile strength measured for the aged samples decreased abruptly for short aging time (below 500 h), but the change became small at a long aging time. Vickers hardness and tensile strength linearly decreased as RIRP decreased, so the degraded mechanical properties of 12Cr ferritic heat resisting steel could be nondestructively evaluated by measuring RIRP.

Experimental Study on the Aerodynamic Characteristics of a Two-Stage and a Counter-Rotating Axial Flow Fan (2단 축류홴과 엇회전식 축류홴의 공력특성에 관한 실험적 연구)

  • Cho, Jin-Soo;Cho, Lee-Sang
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.25 no.10
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    • pp.1281-1292
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    • 2001
  • Experiments were done for the comparison of performance and flow characteristics between a two -stage axial flow fan and a counter-rotating axial flow fan. Each stage of the two -stage axial flow fan used fur the present study has an eight bladed rotor and thirteen slater blades. The front and the rear rotor of the counter - rotating axial flow fan have eight blades each and are driven by coaxial counter ro latins shafts through a gearbox located between the rear rotor and the electric motor. Both of the two axial fan configurations have identical rotor blades and the same operating condition fur the one -to-one comparison of the two. Performance curves of the two configurations were obtained and compared by varying the blade pitch angles and axial gaps between the blade rows. The fan characteristic curves were obtained following the Korean Standard Testing Methods for Turbo Fans and Blowers (KS B 6311). The fa n flow characteristics were measured using a five-hole probe by a non-nulling method. The velocity profiles between the hub and tip of the fans were measured and analyzed at the particular operating condition s of peak efficiency, minimum and maximum pressure coefficients. The peak efficiency of the counter-rotating axial fan was improved about 2% respectively, compared with the two stage axial fan. At the minimum pressure coefficient point of the two stage axial fan, the fan inlet flow patterns show that axial velocity highly decreased in the vicinity of the blade tip region. Also, the reverse flow took place at the blade tip.