• Korean Journal of Ichthyology
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• v.8 no.2
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• pp.68-73
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• 1996

This study was carried out to obtain the basic information on morphological and chromosomal charateristics in the three species of Chinese carps (grass carp; Ctenopharyngodon idellua, bighead carp; Aristichthys nobilis, and silver carp; Hypophthalmichthys molitrix) introduced to Korea from China. C. idellua was differ from A. nobilis and H. molitrix by the number of gill rakers, scales, fin rays, body proportion. A. nobilis and H. molitrix were similar in having ventral keel and many scale number, but H. molitrix was differ from A. nobilis by the connected gill rakers and body color pattern. Diploid chromosome and arm number (fundamental number, NF) of the three species were all the same to 2n=48 and NF=84. Diploid chromosome numbers in the three species are consisted of 10 pairs of metacentric chromosome, 8 pairs of submetacentric chromosome and 6 pairs of acro and/ or telocentric chromosome. Morphological and karyological relationship of the three Chinese carps are discussed.

• Animal Systematics, Evolution and Diversity
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• v.5 no.2
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• pp.107-119
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• 1989

Three species of Korean sticklebacks family Gasterosteidae were reviewed based on the specimens of several populations for the study of their morphological variations and taxonomical positions. All specimens of Gasterosteus aculeatus observed have a complte row of lateral plates ranging from thirty-two to thirty-five in number. The low-plate morph regarded as landlocked type is virtually absent. And no difference was recognized among six populations of G. aculeatus in the meristic chracters, i.e. number of vertebrae, gill rakers and finrays. The Pungitius sinensis and P.kaibarae ssp. shown local variations have scutes ranging from 31 to 35, but not clinal in the morphometric characters. But P.sinensis was statistically different from P.kaibarae ssp. in some characters such as the number of dorsal spine rays and vertebrae, and body depth, although their ranges overlapped. No significant taxonomic difference was detected between white from and black from in dorsal spinous membrane of P.kailbarae ssp. Although these three species of Korean sticklebacks have the same diploid chromosome number (2N=42), G. aculeatus is obviously different from the two species of genus Pungitius in their karyotype ; G. aculeatus consists of six metacentrics, six submetacentrics, and thiry subtelo-acrocentrics, and both P.sinensis and P.kaibarae ssp. have four metacentrics, six submetacentrics, and thirty-four subtelo-acrocentrics.

• The Magazine of the IEIE
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• v.11 no.5
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• pp.42-50
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• 1984

국내의 다음세대 컴퓨터 개발을 위한 VLSI 설계와 CAD 분야에 대한 연구 방향을 제시한다. 연구의 목표는 국제적으로 경쟁할 수 있는 VLSI 설계능력과 백만개 정도의 트랜지스터로 자성된 회로를 경제적으로 설계하기 위한 CAD 기술과 System의 확립이다. ·새로운 회로 구조와 알고리즘에 대한 연구 · CAD 도구와 언어의 개발에 관한 첨단 CAD 기술개발연구 · VLSI 설계에 필요한 CAD 도구 이용과 개발에 필요한 표준 인터페이스, 네트워킹, 컴퓨팅 하드웨어. 시스템 소프트웨어에 대한 연구등의 부분으로 크게 나눌 수 있다. 이용 가능한 CAD system을 평가하고 개선하며 첨단 CAD에 대한 소프트웨어와 하드웨어에 대해 · 컴퓨팅 하드웨어 · 프로그램 분위기 · 네트워킹 능력 ·자료 교환을 위한 표준인터페이스 등에 관해 조사분석도 병행한다. CAD에 관한 세부적인 연구 과제는 · 시스템 사양언어 · 설계 검증 ·시스템시뮬레이션· 설계 합성 · 설계 해석· 설계 방법론·디바이스와 공정 모델링 프로그램 등이다. 고속 계산용 VLSI에 관한 구조와 알고리즘은 행렬 계산을 위한 ·분산 배열 처리 회로 ·시스토릭 (Systolic) 배열 회로 ·셀률라(Cellular) 논리 회로 · 3차원 배열 회로 와 · 비규칙적 계산 알고리즘을 갖는 VLSI가 있다. VLSI설계훈련과 CAD 기술 축적을 위해 CAD enter를 설립하여 전국적인 CAD 네트워킹을 관계 연구소와 여러 대학에 가설하며, MPC 계획을 추진한다. VLSI설계 가능성이 입증되면 VLSI 설계능력을 더욱 향상 시키기 위해 0.5∼1.0mm기술의 silicon faundary를 설립한다. 연구 개발 조직은 대학, 산업체. 연구소가 삼위일체가 되어 수행될 수 있도록 연구 개발 위원회를 설치 운영하며 경쟁적이며 경제적으로 연구 업무를 집행하는 것이 바람직하다.았다.형질에 관여하는 귀전자에 미치는 기구에 대하여 검토할 여타가 있다고 보여진다. 분해능의 특징으로 미루어 앞으로는 레이저를 이용한 계측 방법이 그 주류를 이룰 것으로 사료된다. 우선 본 해설은 기체의 온도 및 농도의 광학적 측정방법중 Raman산란광 검출법에 대하여 실제로 측정하는 입장에서 간단히 소개한다.lity)이, 높은 $GA_3$함량에 기인된다'는 주장은 본실험(本實驗)으로 부인(否認)되었다. 따라서, 응용학적(應用學的) 측면에서 고려해 볼 때, 리베스식물(植物)의 육종기간 단축을 위한 모든 화아분화(花芽分化) 촉진 조치는 P.J.-식물(植物)이 20. node이상 생육하였을 때 취하는 것이 효율적인 것으로 결론 지어진다.앞당겨진 7月 셋째 週였다. 8. Culex (Culex) tritaeniorhynchus summoro년의 最大發生 peak는 1981年, 1982年 모두 8月 둘째 週였다. 9. Anopheles (Anopheles) sinensis의 最大發生 peak는 1981年에 7月 다섯째 週, 1982年은 2週 앞당겨진 7月 셋째 週였다. 10. 重要 3種의 最大 peak를 比城하면 Culex (Culex) pipiens pallens와 Anopheles (Anopheles) sinensis는 1981年과 1982年 모두 最大 peak時期가 同一하였으며, Culex (Culex) tritaeniorhynchus summoro년는 2年間 모두 8月둘째 週에 나타났다.osterior to manubrium and anterior to aortic arch) replacing the normal mediastinal fat. (2) In benign thymoma, the marging of the mass was smooth and the normal fat

• Animal Systematics, Evolution and Diversity
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• v.9 no.2
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• pp.151-170
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• 1993

Morphometric, band-pattern and electrophoretic analysis on Cobitis taenia complex were performed to investigate the morphological and genetic differentiation and to clarify their taxonomic status. Intermediate types of band-pattern (C and D type) were more frequently expressed than that of types of C. t. taenia(type A) and C. t. lutheri (type B). Sexual dimorphism of band-pattern was observed not only in C. t. taenia(type A) and C. t. lutheri(type B). Sexual dimorphism of band-pattern was observed not only in C. t. lutheri but also in C. t. taenia and C. t. striata as well. Discriminant function analysis based on 19 morphological characters shows no significant differences among C. taenia complex. The degree of genic variation of C. t. striata was higher ( =1.48, P=31.2%, HD=0.009) than those of C. t. striata was higher( =1.48, P=31.2%, HD=0.082 and HG=0.009) than those of C. t. lutheri ( =1.37, P=2.7%, HD=0.058 and HG=0.065). The average genetic similarities between C. t. lutheri and C. t. taenia-C. t. striata were S=0.62 and S=0.66 respectively and these values indicate that C. t. tanenia has evolved specific level of differentiation. C. t. striata and C. t. lutheri show subspecifc level of close genetic similarity (S=0.82). Based on the divergent time estimate (Nei, 1975) it is assumed that C. t. tanenia was branched off from the other subspecies about two million years before present (MYBP) and C. t. striata and C. t. lutheri were differentiated about 0.6 MYBP. The use of C. sinesis an the scientific name for the Korean C. t. taenia, proposed by Kim and Lee (1988) seems incorrect since they are quite different in the structure of lamina circularis (Vladycov, 1935), the external morphology and distribution (Cheng and Zheng, 1987) and the chromosome number(Yu et al., 1989). Kim and Lee(1988) also argued that C.t. striata and C. t. lutheri should be treated as distinct species but the present study and other reports (Kim and Lee, 1984; Kim and Yang, 1993) do not support it. We conclude that C. t. taenia is a good species and C. t. striata and C. t. lutheri are subspecific status. Their scientific names should be revised in the future.

• Nuclear Medicine and Molecular Imaging
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• v.43 no.5
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• pp.487-494
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• 2009

Purpose: Small size of recombinant scFv antibody has many advantages such as rapid blood clearances and improved targeting antibodies to tumor region. On the other hand owing to small size, number of amino group is insufficient in conjugation with chelator and fluorescence labeling. This study is to introduce poly lysine tag to the C-terminal end of scFv lym-1 sequence for fluorescence chelator conjugation. Materials and Methods: Poly lysine scFv lym-1 gene, cloned into pET-22b (+) vector, was expressed in E. coli BL21 (DE3) strain. Antibody purification was performed with Ni-NTA column and then size exclusion column chromatography. Expression and purification levels of poly lysine tagged scFv lym-1 antibody were confirmed by western blot analysis. I-124, I-125, I-131 and Tc-99m were used for radiolabeling of purified poly lysine scFv lym-1. Flow cytometry analysis of FIT( conjugated poly lysine scFv lym-1 was performed for confirmation of immunoreactivity of human Burkitt's lymphoma cells. Results: Poly lysine scFv lym-1 antibody was purified through two steps and identified as molecular weight of 48 KDa. Radiolabeling yields of I-124, I-125, I-131 and Tc-99m into poly lysine scFv lym-1 were >99%, >99%, >95% and >99%, respectively. Flow cytometry analysis of poly lysine scFv and scFv lym-1 was showed similar immunoreactivity to human Burkitt's lymphoma cells. Conclusion: Poly lysine tag was useful for the sufficient number of amino groups to scFv lym-1 antibody for chelator conjugation with minimizing loss of immunoreactivity.