• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.495-502
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• 2004

Intimin, the product of eae gene in EHEC O157:H7, is required for intimate adherence. In this study, the C-terminaI region(281 amino acids) of the EHEC OI57:H7 intimin were expressed as a protein fusion with (His)$_6$ which was used to raise antiserum in rabbits. The antiserum reacted in western blot with a 94kDa outer membrane protein of EHEC O157:H7. It was observed that the antibody titers both in egg yolk and serum appeared in 2${\sim}$4 weeks after immunization with fusion protein. At the time of 8 weeks, the titre of egg yolk was found to be higher than that of sera. According to the results of neutralization test, chicken egg-yolk antibody(lgY) against the recombinant intimin strongly reacted to EHEC O157:H7. We conclude that a truncated recombinant intimin could be used as an immunogen to elicit antibody(lgY) against O157:H7.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2015.07a
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• pp.216-217
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• 2015

This study consist of research on the strength development properties of alkali activated slag(AAS) by differential combination of activators, initial protecting and curing conditions. 3 type of binders cured in the atmosphere, underwater and sealed were estimated compressive strength of 3, 7 and 28 days. Test results showed that strength development properties of binders varied with initial protecting and curing conditions because of ionized anions in pore water.

• Journal of the Korea Institute of Building Construction
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• v.21 no.6
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• pp.551-562
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• 2021

In this study, to suggest an efficient method of using coal gasification slag(CGS), a byproduct from integrated gasification combined cycle(IGCC), as a combined fine aggregate for concrete mixture, the diverse performances of concrete mixtures with combined fine aggregates of CGS, river sand, and crushed sand were evaluated. Additionally, using CGS, the reduction of the hydration heat and the strength developing performance were analyzed to provide a method for reducing the heat of hydration of mass concrete by using combined fine aggregate with CGS and replacing fly ash with cement. The results of the study can be summarized as follows: as a method of recycling CGS from IGCC as concrete fine aggregate, a combination of CGS with crushed sand offers advantages for the concrete mixture. Additionally, when the CGS combined aggregate is used with low-heat-mix designed concrete with fly ash, it has the synergistic effect of reducing the hydration heat of mass concrete compared to the low-heat-designed concrete mixture currently in wide use.

• Food Science of Animal Resources
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• v.25 no.2
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• pp.232-237
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• 2005

Lactoferrin is a member of the transferrin family of iron-binding glycoproteins. It is originally found in milk. In addition to its antibacterial and antiviral activities, lactoferrin has many other biological functions include anti-inflammatory properties, antitumor, cell growth-promoting activity as well as antioxidant effect In the present study, we report the production of recombinant bovine lactoferrin and lactoferrin N-lobe in the Rhodococcus erythropolis (R erythropolis) using pTip vector. The expression level was investigated in various range of temperature, and we could successfully expressed the bovine lactoferrin and lactoferrin N-lobe in R erythropolis at low temperature. The recombinant proteins were purified by Nickel-Nitrolotriacetic acid (Ni-NTA). The purified proteins were confirmed by SDS-PAGE and Western blot, which indicating that the recombinant proteins have a molecular weight of 80kDa and 43kDa for bovine lactoferrin and lactoferrin N-lobe, respectively.

• Journal of the Korean Recycled Construction Resources Institute
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• v.2 no.1
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• pp.26-33
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• 2014

The study investigates the effects of the type, replacement ratio and method of use of mineral admixtures on the fluidity, bleeding ratio, volumetric change and compressive strength of the grout in order to provide basic data for the development of high-quality grout for PSC bridges. In view of the results relative to the type and replacement ratio of the mineral admixtures, it appears that fly ash has practically no effect on the improvement of the fluidity nor on the reduction of bleeding and shrinkage of the grout. On the contrary, blast furnace slag and silica fume appear to have significant effect on the improvement of the fluidity or on the reduction of bleeding and shrinkage of the grout. With regard to the combined use of mineral admixtures, the combination of fly ash and blast furnace slag provides satisfactory fluidity but with significant increase of bleeding and shrinkage, whereas the combination of blast furnace slag and silica fume reduces bleeding and shrinkage but with large loss of the fluidity. On the other hand, the combination of fly ash and silica fume results in satisfactory fluidity accompanied with fair reduction of bleeding and shrinkage of the grout. In view of these results, the type, replacement ratio and method of use of the mineral admixtures are seen to influence the fluidity, bleeding and volumetric change of the grout. Accordingly, it is necessary to select the mineral admixtures considering these effects for their exploitation in the grout of PSC bridges.

• The KIPS Transactions:PartD
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• v.13D no.4 s.107
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• pp.593-602
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• 2006

Software development methodology has been developed for satisfying goals of improvement in productivity and reduction in time-to-market through the reuse of software assets. The current methods that implement software product-line, one of software development methodologies, interfere massively with the core assets, which require high cost in assembly level reducing the effectiveness. In this paper, we introduce Aspect-Oriented Programming (AOP) as a method for improving assembly process in software product-line. The method that assembles core assets and variabilities is described by grammar elements such as Join point, pointcut and advice without code-change. We analyze requirements of a mini-system as an example adapting AOP and design using UML. Our study implements the variabilities, which are from design stage, using an Aspect-Oriented Programming Language, AspectJ and prove usability and practicality by implementing the proposed idea using an Aspect-Oriented Programming Language, AspectJ.

• KSBB Journal
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• v.9 no.1
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• pp.8-15
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• 1994

The research has been carried out to optimize a recombinant S. cerevisine fermentation process for the production of an anticoagulant hirudin. The structural gene coding for hirudin was combined with the GAL10 promoter for controlled expression, the MFal signal sequence for hirudin secretion, and the GAL7 terminator for transcriptional termination. Growth medium composition and environmental conditions were optimized for maximizing cell growth and final hirudin concentration. The optimized conditions included yeast extract 40g/$\ell$, casamino acid 5g/$\ell$, g1ucose 20g/$\ell$, galactose 30g/$\ell$, DO 50% and temperature $30^{\circ}C$. These conditions yielded the specific cell growth rate of $0.13hr^{-1}$, the final cell density of 30g cell/$\ell$ and the final hirudin concentration of 64mg/$\ell$ in the batch fermentation with a 2.5$\ell$ jar fermentor.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.4A
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• pp.787-794
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• 2006

Generally, high performance concrete has characteristics such as low water-cementitious material ratio, lots of unit binder powder, thus the heat of hydration, autogenous shrinkage are tend to be increased. This study is to investigated the effect of the expansive additive and shrinkage reducing agent on the shrinkage properties of high performance concrete as a study to develop the reduction technology of the concrete shrinkage. Test results showed that the expansive additive and shrinkage reducing agent were effective the reduction of shrinkage of high performance concrete. Especially, the using method in combination with expansive additive and shrinkage reducing agent was more effective than the separately using method of that. Also, it analyzed that the combination of expansive additive of 5% and shrinkage reducing agent of 1% was the most suitable mixture, considering to the fluidity, strength and shrinkage properties.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 1999.04a
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• pp.30-30
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• 1999

샌드위치 복합재는 단일 부재의 금속재나 복합재에 비해 높은 비강성과 비강도를 얻을 수 있을 뿐 아니라 면재와 코아를 적절하게 조합하면 우수한 피로특성, 단열성, 흡음성 등을 얻을 수 있기 때문에 항공기, 철도차량, 선박, 냉동컨테이너, 건축재 등에 널리 활용되고 있다. 대부분의 샌드위치 복합재는 면재와 코아를 일차 또는 이차 선착을 통해 제작하기 때문에 운용 중 샌드위치 복합재에 가해질 수 있는 충격하중 등으로 인해 면재와 코아 사이의 층간분리, 면재의 인장파괴, 코아의 압축파괴, 코아의 진단파괴 등과 같은 손상이 생길 우려가 있다. 최근에는 샌드위치 복합재가 갖는 이러한 문제점들을 해결하기 위한 방편으로 코아의 양면에 부착된 면재들을 코아의 두께방향으로 스티칭함으로써 서로 일체형으로 결합시킨 형태인 스티칭된 샌드위치 복합재의 개발이 시도되고 있다.

• Journal of Life Science
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• v.18 no.3
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• pp.298-303
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• 2008

Using a phage peptide library approach, we have isolated a peptide ligand that binds to transferrin receptor on the surface of human melanoma cell, B16F10. The library was first screened twice by recovering internalized phages and was further screened three times by competitively eluting transferrin receptor-specific phages with human transferrin among the phages bound to the cell surface. The peptides displayed by the selected phages were fused to translocation and catalytic domain of Pseudomonas exotoxin to prepare recombinant toxins. After estimating cytotoxicity of each recombinant toxin toward B16F10 cell, seven clones were selected. Sequence analysis revealed that one of the clones displayed a peptide which had a significant sequence homology with human transferrin. The peptide was chemically synthesized and was shown to be functional in delivering cytotoxic agents into B16F10 cell via interaction with transferrin receptor.