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Systematic Review on Application of Whey Towards Production of Galacto-oligosaccharide Using β-Galactosidase Enzyme from Pichia pastoris

  • Ramachandran, C;Oh, Deog-Hwan
    • Journal of Food Hygiene and Safety
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    • v.35 no.4
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    • pp.304-311
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    • 2020
  • Galacto-oligosaccharides (GOS) are prebiotics that have a beneficial effect on human health by promoting the growth of probiotic bacteria in the gut, in addition to having various applications in the food industry. GOS are generally produced from lactose in a reaction catalyzed by β-galactosidase. Synthesis of GOS from whey permeate (WP) (ultrafiltration of whey, concentrated then spray dried) using surface engineered β-galactosidase in Pichia pastoris (P. pastoris) is a novel method to convert waste into a valuable product. Cell-surface display is the expression of peptides and proteins on the surface of living cells by fusing them to functional components of cells. Surface engineered cells have many potential uses. The Flo1p flocculation functional domain, thought to be located near the N terminus, recognizes and adheres non-covalently to cell-wall components such as α-mannan carbohydrates, causing reversible aggregation of cells into flocs.

TREATMENT OF PRIMARY AND PERMANENT TEETH WITH THE AIR-ABRASIVE TECHNOLOGY (Air abrasion 기술을 이용한 유치 및 영구치의 수복)

  • Cho, Hyun;Lee, Kwang-Hee;Kim, Dae-Eop;Song, In-Kyung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.2
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    • pp.210-216
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    • 2002
  • Air abrasion technology can prepare enamel and dentin for bonding, similar to etching by acidic gels and solutions. Longer treatment can excavate pit and fissures, preparing the tooth for immediate placement of bonded resin materials. Although not appropriate for every clinical situation, the air abrasive technology minimizes heat, vibration and bone-conducted noise associated with conventional means of caries removal since the cutting is accomplished by air pressure. Also, patients treated with the air-abrasion technology rarely request anesthesia. Air abrasion technology was more effective in treating early carious lesions and stains compared to lesions where caries had already progressed to produce soft dentin and the strong air stream and noise caused by the evacuation system was a major discomfort to pediatric patients, and the experience and skillfulness of clinician should be required for accurate and proper tooth preparation.

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On the Analysis of DS/CDMA Multi-hop Packet Radio Network with Auxiliary Markov Transient Matrix. (보조 Markov 천이행렬을 이용한 DS/CDMA 다중도약 패킷무선망 분석)

  • 이정재
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.19 no.5
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    • pp.805-814
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    • 1994
  • In this paper, we introduce a new method which is available for analyzing the throughput of the packet radio network by using the auxiliary Markov transient matrix with a failure state and a success state. And we consider the effect of symbol error for the network state(X, R) consisted of the number of transmitting PRU X and receiving PRU R. We examine the packet radio network of a continuous time Markov chain model, and the direct sequence binary phase shift keying CDMA radio channel with hard decision Viterbi decoding and bit-by-bit changing spreading code. For the unslotted distributed multi-hop packet radio network, we assume that the packet error due to a symbol error of radio channel has Poisson process, and the time period of an error occurrence is exponentially distributed. Through the throughputs which are found as a function of radio channel parameters, such as the received signal to noise ratio and chips of spreading code per symbol, and of network parameters, such as the number of PRU and offered traffic rate, it is shown that this composite analysis enables us to combine the Markovian packet radio network model with a coded DS/BPSK CDMA radio channel.

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Calculation of Power Distributions on Uranium- and Plutonium-Loaded Cores Moderated by Light Water (우라늄 및 플루토늄 장전 노심에서의 출력 분포 계산)

  • Sang Keun Lee;Kap Suk Moon;Jong-Hwa Jang;Ji Bok Lee;Chang Kun Lee
    • Nuclear Engineering and Technology
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    • v.15 no.4
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    • pp.267-279
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    • 1983
  • An analytical system has been established for scrutinizing both uranium- and plutonium-fueled lattices moderated by light water. This system consists of two primary codes. One is a unit cell program called KICC, which has theoretical foundation on the models of GAM and THERMOS incorporated with appropriate approximate treatments for various phenomena, whereas the other is a multi-dimensional diffusion-depletion program entitled KIDD. The adequacy of this system is verified by performing extensive benchmark calculations on a variety of critical experiments. The average value of effective multiplication factors for the selected nineteen UO$_2$ critical experiments of heterogeneous lattice structure is calculated to be 1.0006 with a standard deviation of 0.0039. Power distributions have also been calculated for some critical experiments fueled with both uranium and plutonium of varying concentrations. The maximum percentage difference between the measured and calculated power distributions appears to be less than 5%. This result, together with the previously reported result, illustrates that the KICC/KIDD system is a very effective tool for the analysis of a light water reactor core.

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Physical Properties of Freeze-Dried Powder of Aloe Vera Gel with Respect to the Concentrating Degree as Pre-Treatment (전처리 농축 정도에 따른 Aloe Vera gel의 동결건조분말의 물성)

  • Lee, Nam-Jae;Lee, Seung-Ju
    • Korean Journal of Food Science and Technology
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    • v.41 no.1
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    • pp.32-36
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    • 2009
  • The physical properties of freeze-dried Aloe vera gel powders were examined according to the influence of the concentration degrees of the gel solutions as raw materials during freeze-drying. As a pre-treatment prior to freeze-drying, the gel solutions were vacuum-concentrated at three concentration levels (g water/g solids): high (H), 76; medium (M), 119; and low (L), 159. The water contents of the three powder samples were almost the same. For their viscosity measurements, non-Newtonian fluid behavior with shear thinning was observed in samples H and M, whereas Newtonian liquid behavior was found in sample L. In electrical conductivity measurements, sample H showed the highest conductivity upon dissolving the powder in water. For their water sorption isotherms, sample H was analyzed to have the least amount of bound water. Finally, it was determined that the degree of concentration caused only slight differences in the physical properties of freeze-dried Aloe gel powders.

Fuzzy Modeling and Fuzzy Rule Generation in Global Approximate Response Surfaces (전역근사화 반응표면의 생성을 위한 퍼지모델링 및 퍼지규칙의 생성)

  • Lee, Jong-Soo;Hwang, Jeong-Su
    • Journal of the Korean Institute of Intelligent Systems
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    • v.12 no.3
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    • pp.231-238
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    • 2002
  • As a modeling method where the merits of fuzzy inference system and evolutionary computation are put together, evolutionary fuzzy modeling performs global approximate optimization. The paper proposes fuzzy clustering as fuzzy rule generation process which is one of the most important steps in evolutionary fuzzy modeling. With application of fuzzy clustering into the experiment or simulation results, fuzzy rules which properly describe non-linear and complex design problem can be obtained. The efficiency of evolutionary fuzzy modeling can be improved utilizing the membership degrees of data to clusters from the results of fuzzy clustering. To ensure the validity of the proposed method, the real design problem of an automotive inner trim is applied and the global approximation is achieved. Evolutionary fuzzy modeling is performed for several cases which differ in the number of clusters and the criterion of rule selection and their results are compared to prove that the proposed method can provide proper fuzzy rules for a given system and reduce computation time while maintaining the errors of modeling as a satisfactory level.

Enhanced Synthesis of Active rPA in the Continuous Exchange Cell-free Protein Synthesis [CECF] System utilizing Molecular Chaperones (분자 샤페론을 사용한 연속확산식 무세포단백질 발현 시스템에서의 재조합 Plasminogen Activator의 효율적 발현)

  • Park, Chang-Gil;Kim, Tae-Wan;Choi, Cha-Yong;Kim, Dong-Myung
    • KSBB Journal
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    • v.21 no.2
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    • pp.118-122
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    • 2006
  • In this report, we describe that the use of GroEL/GroES-enriched S30 extract remarkably enhances the solubility and enzymatic activity of cell-free synthesized rPA, which requires the correct formation of 9 disulfide bonds for its biological activity. We found that the stable maintenance of redox potential is necessary, but not sufficient for the optimal expression of active rPA. In a control reaction without using additional molecular chaperones, most of the rPA molecules were aggregated almost instantly after their expression and thus failed to exhibit the enzymatic activity. However, by the use of GroEL/GroES-enriched extract, combined with IAM-treatment, approximately $30{\mu}g/ml$ of active rPA was expressed in the cell-free synthesis reaction. This result not only demonstrates the efficient production of complex proteins, but also shows the control and flexibility offered by the cell-free protein synthesis system.

Structural Construction of MVC Applications using Distributed Object Composer (분산 객체 조립기를 이용한 MVC 응용의 구성적 작성)

  • Eum, Du-Heon
    • Journal of KIISE:Software and Applications
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    • v.26 no.11
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    • pp.1298-1305
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    • 1999
  • 네트워크 및 분산 처리 기술의 발달로 분산 환경에서 동작하는 응용 프로그램에 대한 생산성 향상이 요구되고 있다. 본 논문에서는 MVC(Model, View, Controller) 패턴을 기반으로 하는 분산 응용 프로그램의 생산성 향상을 위해, 조립식 분산 응용의 작성을 지원하는 분산 객체 조립기, DOC(Distributed Object Composer)를 소개한다. 분산 객체 조립성의 기반이 되는 DOC의 구성적{{) '전체'를 창조해 내기 위해 여러 요소들을 결합, 배치하여 하나의 예술작품을 성립시키는 방법. 모아서 조립한다는 의미.}} 인터페이스(Structural Interface)는 기존 객체지향 기술의 절차적 인터페이스(Procedural Interface)에 비해 콤포넌트 재사용성을 개선하고, 분산 응용의 신속한 개발을 지원하여, 소프트웨어의 생산성 및 확장성을 향상시킨다. 또한, DOC 방식으로 모델링하고 구현한 공급망 관리(Supply Chain Management: SCM) 응용 프로그램의 조립식 작성을 통해, DOC의 실시간 정보 처리 및 조립 기능을 보인다.DOC는 이질적인 통신 환경에서 클라이언트와 서버 객체간의 네트워크 투과성을 제공하는 미들웨어, CORBA를 기반으로 한다. 또한, 분산 객체들 간의 상호작용을 자동 처리하기 위해 Java의 관찰자/피관찰자(Observer/Observable)를 확장한 분산 관찰자/피관찰자 패턴을 정의하여 DOC와, 서버 및 클라이언트의 분산 응용 객체들을 정의하기 위한 인터페이스에 적용한다.Abstract Due to the availability of advances in network and distributed processing technologies, the enhanced productivity of application software that runs on distributed environment is demanded. In this paper, we introduce the Distributed Object Composer (DOC) that supports the structural composition of distributed applications for the enhanced productivity of MVC (Model, View, Controller) pattern-based application programs. The structural interface of DOC that is the basis for the composibility of distributed objects increases the reusability of software components and provides better software productivity and extendibility than the procedural interface of the traditional object-oriented technology by supporting rapid constructions of distributed applications. In this paper, we also discuss the real-time information processing and composibility features of DOC by applying the DOC mechanism to the Supply Chain Management (SCM) application.DOC is implemented using the CORBA middleware that supports the network transparency between client and server objects on heterogeneous communication environments. We apply the distributed Observer/Observable pattern that extends the Observer/Obseverable of Java to DOC itself and distributed client/server objects as their interface definitions for the automatic interactions of distributed objects.

A Evaluation of Corrosion Resistance on the Thermal Sprayed Coating Tube and Cladded Tube of the Open Rack Vaporizer (용사코팅튜브와 클래드튜브 기화기에 대한 내식성 평가)

  • Baek Jong-Hyun;Lee Jae-Ho;Kim Yong-Seog;Shin Dong-Hyuck;Kim Woo-Sik
    • Journal of the Korean Institute of Gas
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    • v.2 no.4
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    • pp.67-72
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    • 1998
  • Due to excellent corrosion resistance, in particular against sea water, Al-2wt.$\%$Zn alloys have been used as the sacrifice anode material for tube of the ORV. Al-2wt.$\%$Zn alloys thermal-sprayed coating, however, were spalled on the lower part of ORV due to the lack of bonding strength between base material and coating layer and the dropping energy of sea water, To overcome the problems of the thermal spray coating, we developed the cladded tube manufacturing process by coextrusion of the clad and base metal. The corrosion resistance of cladded tube was improved by two times at least, compared with that of the present thermal spray coated vaporizer tubes.

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Development of Sensitivity-Enhanced Detector using Pixelization of Block Scintillator with 3D Laser Engraving (3차원 레이저 각인으로 블록형 섬광체의 픽셀형화를 통한 민감도 향상 검출기 개발)

  • Lee, Seung-Jae;Baek, Cheol-Ha
    • Journal of the Korean Society of Radiology
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    • v.13 no.2
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    • pp.313-318
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    • 2019
  • To improve the sensitivity, a detector using a block scintillator was developed. In the pixelated scintillator, a reflector is located between pixels to move the light generated from the scintillator to the photosensor as much as possible, and sensitivity loss occurs in the reflector portion. In order to improve the sensitivity and to have the characteristics of the pixelated scintillator, the block scintillator was processed into a scintillator in pixel form through three-dimensional laser engraving. The energy spectra and energy resolution of each pixel were measured, and sensitivity analysis of block and pixel scintillator was performed through GATE simulation. The measured global energy resolution was 20.7%, and the sensitivity was 18.5% higher than that of the pixel scintillator. When this detector is applied to imaging devices such as gamma camera and positron emission tomography, it will be possible to shorten the imaging time and reduce the dose of patient by using less radiation source.