This study investigated the microbial contamination levels of raw meats used for short-ripened salami and changes in the microbial and physico-chemical properties of the product during storage at 10 and $25^{\circ}C$ for up to 120 days. The microbial counts of raw meats ranged between 2 and 4 Log CFU/g. Frozen-thawed sow meat showed higher total aerobe and Enterobacteriaceae counts than fresh chilled pork and pork back fat. Staphylococcus aureus was found in all raw materials except fresh chilled pork samples, and Clostridium perfringens was detected in a sample stored for 21 days at $25^{\circ}C$. The counts of total aerobes, lactic acid bacteria and Staphylococcus spp. decreased more rapidly at $25^{\circ}C$ than at $10^{\circ}C$ when the storage time was extended. The growth of Enterobacteriaceae, Pseudomonas spp., Clostridium spp., yeast, and mold were restricted to levels below 2 Log CFU/g during storage. The contents of salt, water, crude protein, crude fat, and ash of salami samples were 3.4, 33.4, 30.8, 32.7, and 4.3%, respectively, which were not affected by storage time or temperature. The pH value of the salami was initially 4.79 and increased to 5.02 and 5.26 after 120 days of storage at 10 and $25^{\circ}C$, respectively, whereas the water activity values decreased from an initial value of 0.91 to 0.90 and 0.88 after 120 days at 10 and $25^{\circ}C$, respectively. The TBA and VBN values increased slowly during storage. The redness value of the salami samples stored at $25^{\circ}C$ decreased more significantly than the samples stored at $10^{\circ}C$. With increased storage time, the values for the rheological characteristics of the salami in terms of hardness, brittleness, elasticity, cohesiveness, gumminess, and adhesiveness tended to decrease more remarkably at $25^{\circ}C$ than at $10^{\circ}C$. Based on sensory evaluation scores, it appears that short-ripened salami is no longer acceptable after 90 days at $10^{\circ}C$ and 30 days at $25^{\circ}C$.
Background : The antitumor effects of herpes simplex virus thymidine kinase (HSV-tk) and ganciclovir (GCV) strategies for cancer gene therapy have a the following advantages : 1) a direct cytotoxicity to HSV-tk modified cancer cells by GCV 2) a cell death by the local transfer of toxic metabolites from the HSV-tk modified cells to nearby unmodified tumor cells (bystander effect), and 3) in vivo bystander effect such as antitumor-immunity. Retroviral and adenoviral sequences can silence transgene expression in cells and mice. In this study, we investigated the above described advantages of HSV-tk/GCV strategy in Lewis lung cell and mouse lung cancer model using retroviral vector and adenoviral vector. Also, we observed whether the expression of a silenced gene can be reactivated by treating cells with butyrate. Methods : Retrovirus-HSV-tk and adenovirus-HSV-tk vectors were used for the transduction of Lewis lung carcinoma (LLC) cells. The change of HSV-tk expression by butyrate was measured by Western blol The antitumor activities containing bystander effect were observed in vivo (by MTT assay) and in vivo tumor models of various combinations of LLC and LLC-tk. Results : 1. Butyrate induced the enhancement of HSV-tk expression from adenovirally transduced cells but not from retrovirally transduced cells. 2. Both retrovirus-HSV-tk and adenovirus-HSV-tk vectors with GCV treatment were effective for killing of tumor cell in vitro and suppression of LLC tumorigenicity. Bystander effect was responsible for killing of mixture of LLC-tk and LLC in vitro and in vivo-tumorigenicity model. Conclusion : Butyrate could augment adenovirus-mediated HSV -tk gene expression. Cancer gene therapy with HSV-tk suicide gene by retroviral and adenoviral vector seems to be an effective approach for lung cancer therapy.
Taxonomic study of the five species (Acanthogobius elongata, A. flavimanus, A. lactipes, A. luridus and Synechogobius) from Korea was carried out based on morphometric, cephalic sensory canal and ecological characters. Taxonomic revision and classificational keys are provided. Synechogobius hasta is easily distinguished from four species of the genus Acanthogobius in eleven characters, i. e., the number of dorsal and anal fin rays, the transverse scales, the vertebral numbers, the formula of interneural spine of the first dorsal fin, the number of interhemal spine anterior to the first hemal spine, the number of epipleural and pleurals, the ratio of caudal peduncle length, the ratio of caudal peduncle depth and the regular variations in the ratio of body parts with the body length. In the genus Acanthobobius, A. elongata is distinguished from other 3 congeneric species in the ratio of body parts and the oculoscapular sensory canal. Moreover, A. flavimanus differs from other 3 congeneric species in the lateral scales, the transverse scales, the number of predorsal scales, the vertebral number, the number of epipleural and pleurals. Sensory papillae rows of S. hasta is not similar that of the genus Acanthogobius in having a singular sensroy papillae rows. A. elongata has no oculoscapular sensory canal D and A. flavimanus has transverse sensory papillae in cheek, and these are one of the unique characters distinguished form other congeneric species. In the spawning period inferred from gonadosomatic index, A. elongata varied from late March to late June ; A. flavimanus, January to April ; A. lactipes, May to September ; A. luridus, early May to early July and S. hasta, early March to early May.
Park, In-Seok;Seol, Dong-Won;Im, Soo-Yeon;Park, Min Ouk;Hur, Woo June;Cho, Sung Woan;Song, Young-Chae;Kim, Jea-Soo;Jo, Hyo-Jae;Noh, Choong Hwan;Choi, Hee Jung
Korean Journal of Ichthyology
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v.19
no.2
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pp.107-111
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2007
Sex differentiation of the brown croaker Miichthys miiuy (Basilewsky) is described from hatching to the 120th day post-hatching (dph) (water temperature $24^{\circ}C$). Primordial germ cells (PGCs) were observed on the 20th dph (10.4 mm total length (TL), 0.14 g body weight (BW), and began to protrude into the peritoneal cavity from the 40th dph (19.4 mm TL, 0.39 g BW). On the 65th dph (31.3 mm TL, 0.93 g BW, $1,560D^{\circ}$ (degree-days)), initial ovarian differentiation was identified by the PGCs with condensed chromatin, and their transformation into meiotic oocytes. By the 120th dph (4.60 mm TL, 1.38 g BW, $2,880D^{\circ}$), the oocytes were in the perinucleolus stage and had increased from 20 to $40{\mu}m$ in diameter. While ovaries gradually grew after sex was differentiated, testes continued to multiply from the 65th dph. On the 80th dph (37.9 mm TL, 1.39 g BW, $1,920D^{\circ}$), the beginning of testis lobule formation was indicated by the occurrence of spermatogonial cysts enveloped by somatic cells in some of the testes. On the 120th dph, the testis lobules of some of the fish contained all germ cell stages through to the spermatocytes. Therefore, the sex differentiation type of the brown croaker is identified as gonochoristic.
Herbicides have been used to control weeds for decades. If detoxification upon exposure to herbicides requires considerable amounts of energy, it could affect the pattern of resource allocation to growth and reproduction of crops. We examined the effects of three levels of a herbicide (Control, Low, and High) on germination, growth and reproductive characters of Glycine max treated twice, i.e., before and after seed germination. Since flowering time of G. max was separated into two groups, flowering time was also considered as a variable in this study. The rate of seed germination tended to be higher at the low level of herbicide compared to other levels. Chlorosis and shape variation of leaves were apparent after the second herbicide treatment, but completely disappeared after six weeks of treatment. The herbicide effects on growth characters were somewhat different between early and late flowering plants, but plants treated with both low and high levels of herbicide reduced their growth compared to those in the control group regardless of flowering time. Plants at the high level of herbicide exhibited the highest growth rate later in the season, suggesting that plants compensated to some extent for reduced growth. However, growth reduction among plants at the high level of herbicide was persistent until the end of growing season. Among plants flowered late in the season, plants in the control level bore a higher number of nodules per plant than those in other levels; such a pattern did not exist among plants flowered early in the season. Plants treated with low and high levels of herbicide produced a lower number of flowers than those in the control. Thus, the herbicide examined affected not only the growth and reproductive characters of non-target crops but also the development and growth of root nodules.
This study was undertaken to understand the seasonal changes of hormone levels such as luteinizing hormone (LH), follicle stimulating hormone (FSH) and estradiol-$17{\beta}$, serum components such as albumin, total protein and triglycerides, and GSI of rainbow trout (Onco-rhynchus mykiss) under the normal-light circumstances. LH levels were the highest in November whereas FSH levels were decreased progressively from October to February. Serum GTH, such as LH and FSH, levels were low immediately after egg-stripping and were significantly increased in the summer in association with the initiation of vitellogenesis. Serum albumin, total protein, and triglycerides were significantly increased in August in association with initiation of vitellogenesis and were peak in November prior to egg-stripping. Oocyte growth, measured by oocyte diameter and GSI, was significantly increased in August and showed the highest value in January. Correlation coefficients of serum LH with FSH and estradiol-$17{\beta}$ were +0.844 and +0.947, respectively. Correlation coefficients of estradiol-$17{\beta}$ with albumin and total protein were +0.634 and +0.859, respectively. Correlation coefficients of serum estradiol-$17{\beta}$ with triglycerides and GSI were +0.673 and +0.694, respectively. Probably by positive feedback mechanism, LH, FSH and estradiol-$17{\beta}$ appeared to stimulate to liver to secrete albumin, total protein and triglycerides for vitellosenesis.
The experiment was performed to evaluate the possibility of utilizing underground sea water for the seed production of tiger puffer, Takifugu rubripes. For this purpose, the effects of 6 different salinities (3.5, 7.0, 14.0, 20.0, 27.0, $33.0\%_{\circ}$) were determined based on the hatching rate of fertilized eggs, survival rate and the amount of food consumed by hatched larvae, where as the effects of 3 different salinities (20.0, 27.0, $33.0\%_{\circ}$) were also examined with rearing tiger puffer juvenile ($4.29{\pm}0.50$ cm in total length) for 50 days in the closed recirculating water system. As a results, either the hatching or the survival rate of more than $70.0\%$ were obtained from the fertilized eggs reared at the salinity of 27.0 to $33.0\%_{\circ}$, the early hatched larvae at 27.0 to $33.0\%_{\circ}$, and the 10-day-old larvae at 20 to $33.0\%_{\circ}$. At three different salinities, the survival rate of 20-day- and 30-day-old larvae turned out to be $89.0\%$ and $92.5\%$, respectively. The salinity for maximum food intake thus appeared to be from 27.0 to $33.0\%_{\circ}$. In this condition, 20-day-old hatched larvae consumed $323\~342$ Artemia nauplii and 30-day-old hatched larvae ate $1,559\~1,5791$ A. nauplii. The highest growth rate of fingerlings were observed at the salinity $27\%$ and followed by 33.0 and $20.0\%_{\circ}$, respectively. The relationship between the days of rearing (X) and the total length (Y) of the fingerlings were as follows : $33.0\%_{\circ}$ group : Y=0.107X-2.532 (r=0.982) $27.0\%_{\circ}$ group : Y=0.116X-3.195 (r=0.975) $20.0\%_{\circ}$ group : Y=0.116X-2.693 (r=0.987) The slopes of regression line estimated from $27.0\%_{\circ}$ and $33.0\%_{\circ}$ groups were significantly different from that of $20.0\%_{\circ}$ group.
Asia-Pacific Journal of Business Venturing and Entrepreneurship
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v.15
no.5
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pp.13-35
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2020
Startup accelerators are a new type of investors providing a certain amount of shares for imparting education, mentoring, networking, and providing space and seed money that can directly resolve the difficulties faced by nascent entrepreneurs (Clarysse, 2016). Startup accelerators have expanded worldwide as their influence over the startup ecosystem has increasingly been established (Pauwels et al., 2016; Cohen & Hochberg, 2014). This study was conducted to derive investment determinants of startup accelerators that are emerging as major investment players around the world. To this end, the accelerator-type determinants of investment were derived. As previous research on this topic is nonexistent, this process involved qualitative meta-synthesis, literature reviews, observation, and in-depth interviews. First, more than 30 research papers were examined for the determinants of investment for firms at an early stage of their foundation, and the categories and determinants of investment in the relevant studies were comparatively analyzed using qualitative meta-synthesis. Further, related data were investigated to identify the characteristics of accelerators, and the startup evaluation process of US accelerators was studied. The more than 100 questions raised during this process were coded to examine the determinants of investment that accelerators considered important. In-depth interviews were conducted with four US accelerators to identify the characteristics of accelerators and key determinants of investment. Ultimately, 5 categories of accelerator-type determinants of investment and 26 subordinate determinants of investment were derived. The results were verified and supplemented by consulting with seven accelerators in Korea. The results were confirmed after pilot tests and verification by seven domestic accelerators. After confirming the accelerator-type determinants, the reliability of them was verified by examining the importance and priority of each category through the quantitative survey of Korean accelerators. The research that elicited the accelerator-type investment determinants is the first research and is expected to be a major reference to the progress of subsequent studies. This research that systematically derived the investment determinants of the accelerator is expected to make major contributions to the progress of follow-up studies, the process of selecting startups, and the investment decision-making process of the accelerators.
Asia-Pacific Journal of Business Venturing and Entrepreneurship
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v.15
no.5
/
pp.77-98
/
2020
Recently, the government provided about 35 trillion won to start-ups through a large amount of start-up support funds from the Korea Credit Guarantee Fund and the Korea Development Bank, and more than 100 trillion won to start-ups over the past three years.(2015-2018) The Ministry of SMEs and Startups announced that a total of 1.4517 trillion won will be supported by 16 ministries (90 projects) after surveying start-up support projects by ministries in 2020 to create a start-up country. Indeed, among the budgets supported by the Ministry of SMEs and Startups, startups accounted for 85%, while scale-ups (growth and maturity) were only 15%. However, the survival rate of Korean start-ups was far lower than that of developed countries. The survival rate for the three years after the start-up was 39.1 percent, with only about 4 out of 10 surviving, with 27.5 percent surviving for five years, and only 3 out of 10 surviving. On the other hand, overseas start-ups have a survival rate of twice as high as that of Korea for five years. Accordingly, this study aims to empirically analyze and examine the need for research on nascent entrepreneurs, which have recently been actively supported by the government, as well as the impact of start-up motivation and start-up preparation on the intention to continue business. The survey was conducted from April 1 to May 15, 2020 for nascent entrepreneurs nationwide, and 222 of the total 245 copies were analyzed, and the questionnaire was measured using the Likert 5-point scale. As a result of the analysis, first, all factors constituting start-up motivation were adopted by significantly affecting start-up satisfaction. In addition, among the sub-factors of nascent entrepreneurs' preparation, item preparation was adopted as a significant influence on start-up satisfaction. Second, the nascent entrepreneurs' satisfaction level was adopted by having a significant impact on the business continuity. Third, some of the motivations for self-realization of the motivation for start-ups were adopted by having a significant impact on the business continuity. In addition, the preparation of items for the start-up preparation of nascent entrepreneurs was partially adopted as they had a significant impact on the business continuity. Fourth, it was adopted by the mediation effect of start-up satisfaction between the motivation of the nascent entrepreneurs' preparation and the business continuity. In addition, it was adopted by the medium effect of start-up satisfaction between the nascent entrepreneurs' preparation and the business continuity. Based on the results of these studies, it is intended to draw practical implications, and to provide useful policy implications to increase nascent entrepreneurs' satisfaction and continue to carry out their start-ups.
Purpose: The object of this study was to evaluate the efficacy of early total parenteral nutrition with early amino acid in extremely low birth weight infant (ELBWI). Methods: We retrospectively analyzed the medical records of all ELBWIs who were born and admitted to Samsung Medical Center from January 2003 to December 2003 and January 2009 to December 2009 and alive at the time of discharge. Data for nutritional status and morbidities were compared between period 1 (2003, n=22), in which parenteral nutritional support was started gradually over several days and period 2 (2009, n=38), in which parenteral nutrition with amino acid was started as soon as possible after birth. Results: Compared to period 1, birth weight and Apgar score were lower in period 2. The intake amount of glucose, amino acid and total calorie was higher and the level of blood urea nitrogen was increased more from 7th day to 14th day after birth in period 2 when compared than period 1. The weight gain velocity was faster at 7th and 14th postnatal day in period 2. There were no differences in the incidence of necrotizing enterocolitis, moderate to severe bronchopulmonary dysplasia, and severe intraventricular hemorrhage (${\geq}$ Gr III) between two periods but, the incidence of periventricular leukomalacia(PVL) was significantly lower in period 2. Conclusion: Early initiation of total parenteral nutrition with early amino acid in ELBWIs was beneficial at weight gain with lowering catabolism and increasing anabolism. And it could be related with reducing the incidence of PVL.
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