• Journal of Life Science
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• v.31 no.4
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• pp.400-409
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• 2021

Sanguinarine, a natural benzophenanthridine alkaloid, has been considered a potential therapeutic target for the treatment of cancer because it can induce apoptosis in human cancer cells; however, the underlying mechanisms of action still remain unclear. Tumor suppressor p53 deletion or mutation is an important reason for the resistance of colorectal cancer cells to anticancer agents. Therefore, in the present study, the role of p53 during apoptosis induced by sanguinarine was investigated in p53wild type (WT, p53+/+) and p53null (p53+/+) HCT116 colon carcinoma cells. Sanguinarine significantly caused greater reductions in cell viability in HCT116 (p53+/+) cells than in HCT116 (p53-/-) cells. Consistently, sanguinarine promoted more DNA damage and apoptosis in HCT116 (p53+/+) cells than in HCT116 (p53-/-) cells while increasing the expression of p53 and cyclin-dependent kinase inhibitor p21^WAF1/CIP1. Sanguinarine increased the activity of caspase-8 and caspase-9, which are involved in the initiation of extrinsic and intrinsic apoptosis pathways, respectively, and it activated caspase-3, a typical effect caspase, in HCT116 (p53+/+) cells. Sanguinarine also increased the generation of reactive oxygen species (ROS), and the Bax/Bcl-2 ratio, while destroying the integrity of mitochondria in HCT116 (p53+/+) cells, but not in HCT116 (p53-/-) cells. Overall, the results indicate that sanguinarine induced p53-dependent apoptosis through ROS-mediated activation of extrinsic and intrinsic apoptotic pathways in HCT116 colorectal cancer cells.

• Korean Journal of Clinical Laboratory Science
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• v.54 no.1
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• pp.9-14
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• 2022

Ulcerative colitis is a disease that causes inflammation in the mucosal or submucosal layer of the colon. Previous studies have reported that obesity increases the prevalence of ulcerative colitis and aggravates the progression. This study was therefore undertaken to investigate whether curcumin inhibits the progression of ulcerative colitis caused by obesity. Mice were bred on a high-fat diet to induce obesity, and curcumin was administered with the high-fat diet to confirm the anti-inflammatory effect. To induce ulcerative colitis, dextran sulfate sodium (DSS) was administered orally, and clinical symptoms of colitis were subsequently observed. For histological evaluation of curcumin, the colon, liver and abdominal fat tissue samples were prepared and analyzed by hematoxylin and eosin (H&E) and Alcian blue-periodic acid-Schiff (PAS) staining. Our results confirm that consumption of curcumin resulted in decreasing the score of the disease activity index, and inhibited shortening of the colon length. In addition, inflammatory cell infiltration and mucosal damage were inhibited in the colon tissue of ulcerative colitis exacerbated by obesity. We further confirmed that exposure to curcumin significantly reduced the steatosis area of the liver and adipocytes of abdominal fat. In conclusion, we believe that curcumin can be applied as a therapeutic agent to treat ulcerative colitis, by inhibiting the progression of colitis caused by obesity.

• The Science & Technology
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• no.6 s.409
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• pp.8-9
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• 2003

당뇨병 환자, 걸어야 오래 산다/ 고섬유 식사, 결장암 예방효과 확인/ 자동차 배기가스, 정자 질 떨어드려/ 야간 조명 노출, 암과 연관있어/ 성별 다른 쌍둥이 사회성 탁월/ 납 노출, 아이들 지능 저하시켜/ 심장병 막으려면 고강도 운동 필요

• Nuclear Medicine and Molecular Imaging
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• v.41 no.6
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• pp.574-577
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• 2007

• Journal of Korean Academy of Nursing
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• v.39 no.4
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• pp.539-548
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• 2009

Purpose: This study was designed to construct and test the structural equation model on sexual satisfaction in patients with a colostomy. Methods: The model construction was based upon Roy's adaptation model. Stoma-related discomfort (SRD), age, frequency of sexual intercourse, treatment modality, and gender affect sexual satisfaction and are mediated by physical, psychosocial, and interdependence modes. Each mode was conceptualized as sexual function, body image/ depression, and marital intimacy. The patients were 112 colostomates with colorectal cancer who were asked to complete a mail-back survey on their demographic data, SRD, body image, depression, marital intimacy, sexual function, and sexual satisfaction. Data were analyzed using SPSS WIN 15.0 and AMOS WIN 7.0. Results: Significant variables for sexual satisfaction in the final model were body image affected by SRD, depression affected by body image and SRD, marital intimacy affected by depression, and sexual function affected by marital intimacy. Conclusion: The results of this study suggest that specific guidelines for SRD are necessary to improve sexual satisfaction among colostomates. Nurses should be vigilant in monitoring depression and body image disturbance, and providing appropriate interventions to increase marital intimacy. Treatment modality, gender, and age should be considered in developing education programs pertaining to sexuality.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.16 no.4
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• pp.506-514
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• 2009

Purpose: This study was conducted to define factors influencing psychosocial adaptation of patients with a permanent colostomy. Independent variables including perceived stress, stoma care self-efficacy, self-care behavior, self-esteem and family support were used to predict psychosocial adaptation. Method: The data was analyzed using the SPSS pc program window version 12.0 for descriptive statistics, Pearson correlation coefficients and stepwise multiple regression. Ninety patients with a permanent colostomy were recruited between September 2007 and May 2008. The reliability of the instruments was tested with Cronbach's alpha which ranged from .78 to .92. Result: The results were as follows: The mean psychosocial adaptation score was 3.05. There were significant correlations between all the predictive variables and psychosocial adaptation (r= .63~ -.43, p<.001). Stepwise multiple regression analysis showed that self-esteem (40%), stoma care self-efficacy (7%), perceived stress (2%), and self-care behavior (2%) accounted for 51% of the variance in psychosocial adaptation. Conclusion: These findings indicate that to improve psychosocial adaptation of patients with permanent colostomy, the major related factors identified in this study should be considered.

• Journal of the Korean Geographical Society
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• v.34 no.5
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• pp.543-560
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• 1999

본 연구는 전국 6개 광역시 및 각 도별로 우리 나라 주요 암의 지역적 분포 현황을 살펴보고 그러한 지역차의 원인을 규명해 보고자 한다. 전국 시 도별 암 등록 현황 자료를 토대로 위암, 직장.결장암, 간암, 폐암과 유방암의 전체 암에 대한 시 도별 상대적 위험도를 분석하여 지리정보체계(GIS)를 이용한 지도화를 근간으로 분석하였다. 위암의 경우 위암 발생의 상대적 위험도는 해당 지역 병원이 부담해야 하는 인구수와 관련성이 있는 것으로 보여졌다. 또한, 폐암의 경우도 대도시지역이 상대적으로 낮은 발병율을 보였으며, 농촌지역은 폐암의 고위험지역으로 나타났다. 이러한 폐암 환자의 분포 현황은 도시 지역에 비해서 농촌 지역의 흡연율이 높은 것과 밀접한 관련성이 있는 것으로 보인다. 직장.결장암의 상대적인 위험도도 도시지역보다는 농촌지역에서 높았다. 이러한 분포 패턴은 농촌지역의 노령화 현상과 관계가 있는 것으로 보인다. 그렇지만, 유방암의 경우 농촌지역보다 대도시지역에서 높은 발암율을 보였다. 이것은 유방암 발생이 식생활을 포함하는 서구화된 생활방식과 관련성이 있음을 시사해 주는 것이다. 간암은 부산, 경남 및 전남 지역에서 높은 환자 빈도를 나타냈다. 특히, 이 해안지역에서의 간암의 상대적으로 높은 위험성은 ‘회’의 섭취로 인한 감염성 질환과 관련이 있는 것으로 보인다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.2 no.2
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• pp.256-261
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• 1999

Ileus caused by impaction of hardened residual barium stagnated in the colon, a rare complication of barium-enema examination, constitutes a particularly serious problem when it occurs in congenital megacolon patients. The administration of gastrografin in such case has proven efficacy in dissolution of impacted barium refratory to routine means of evacuation. We experienced a case of barium-impaction occurred in 6-months old male who had congenital megacolon. Gastrografin enema was a safe and simple method used in the management of this case.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.7
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• pp.832-838
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• 2009

The inhibitory effects of solvent extracts from dried beet (Beta vulgaris) on $H_2O_2$-induced oxidative stress in cell systems and on the growth of cancer cell lines (HT-29 human colon cancer and AGS human gastric adenocarcinoma cells) were investigated. Inhibitory effects of acetone with methylene chloride (A+M) and methanol (MeOH) extracts on the growth of HT-29 and AGS cancer cells increased in a dose dependent manner (p<0.05). The inhibitory effect was more significant on the growth of AGS cells and A+M extracts had a higher inhibitory effect compared to MeOH extracts. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of both cancer cells (p<0.05). Among fractions, hexane and 85% aq. methanol fractions showed higher inhibitory effects. In order to determine the protective effect on $H_2O_2$-induced oxidative stress, DCHF-DA (dichlorodihydrofluorescin diacetate) assay was conducted. The A+M and MeOH extracts of dried beet appeared to significantly reduce the levels of intracellular (ROS) with dose responses. Among the fractions, 85% methanol fractions showed a higher protective effect on production of lipid peroxides. These results indicate that the intake of dried beet may improve oxidative stress in cell and reduce cancer risk.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.6
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• pp.720-726
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• 2007

This study was performed to investigate effects of raw sea tangle products on idiopathic constipation. A total of thirty-eight women participated voluntarily in this study. They were placed into one of four groups by their total colonic transit time (TCTT) to make the four groups have the same TCTT. In the first trial, the subjects in Control group were fed 3 times of 150 mL of mineral water per day for 2 weeks, those in +Control group were fed a kind of stool softener as well as the same mineral water for 3 times, those in STT group were fed 3 times of 150 mL of sea tangle tea and those in STB group were fed 3 times of 150 mL of sea tangle beverage. After 6 weeks, a second trial was performed for another 2 weeks. Although the TCTT of the group exposed to of KolomarkTM was not significantly reduced, the subjects in STT group answered that their evacuation activities were significantly improved; evacuation frequency was increased, stool hardness was reduced, evacuation straining was lessened, stool amount was increased, incomplete sense of evacuation was lessened, and major evacuation time was improved like those in +Control group. The results imply that it is worthy to develop some products of raw sea tangle such as STT, which are effective in treating or preventing constipation.