• Economic and Environmental Geology
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• v.40 no.5
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• pp.661-673
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• 2007

Rock materials of the three-storied stone pagoda in the Cheongryongsa temple in Korea are mainly composed of gneissose two-mica granite and fine-grained granite. This stone pagoda shows structural instability due to cracks and breaking-out of the stones. The surface properties of the stone is highly degraded by various inorganic pollutants and epilithic biospecies. Therefore, this study carried out comprehensive deterioration diagnosis by non-destructive methods, and some conservation treatments base on the diagnosis were carried out to reduce weathering progress. As the treatments, the biospecies and lichen that covering on the stone surfaces were removed by dry and wet cleaning, and degraded concrete applied to the pagoda for restoration in the past was removed and repaired with epoxy resin. Oxidized iron plates inserted between the rock properties were also substituted titanium stainless steels. After all processes are completed, we sprayed consolidant on the rock surface. Finally, the ground of the stone pagoda was rearranged using small rock aggregates, and the fence was established for control of artificial deterioration by visitors and environmental maintenance.

• Journal of Animal Science and Technology
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• v.46 no.6
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• pp.937-946
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• 2004

Duroc is widely used to improve the meat quality and productivity. To elucidate the phylogenetic relation and the sequence specificity for the maternal property, the complete sequence of mitochondrial genome was determined and the population diversity of Duroc was investigated in this study. The length of mtDNA tested is 16,584-bp. There are several insertion/deletion mutations in the control region and coding regions for tRNA and rRNA, respectively, but not in peptide-coding regions. Four peptide-coding genes(COⅡ, COⅢ, ND3 and ND4) showed incomplete termination codon sequences such as T--, and two(ND2 and ND4L) did alternative initiation codons(AIC), respectively. Especially, the initiation codon sequences of ND2 gene were polymorphic in this population. Polymorphisms were detected in 11-bp duplication motif within control region as well as ND2 and CYTB. Variation patterns observed from the tests on three mtDNA regions were linked completely and then two haplotypes obtained from combining the data dividing this population. Duroc mtDNA is observed at the European pig cluster in the phylogenetic tree, however, the results from the population analyses supported previous opinions. This study suggests that the breed Duroc was mainly originated from the European pig lineage, and Asian lineage was also used to form the pig breed Duroc as maternal progenitors.

• Journal of Life Science
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• v.23 no.1
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• pp.24-30
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• 2013

The purpose of this study was to identify genetic polymorphisms of the mitochondrial cytochrome b (mtDNA cyt b) gene in Korean black (KB) cattle breed and to analyze the genetic relationship between the KB and other breeds. We determined the complete sequence of the mtDNA cyt b gene in 38 KB cattle. We also analyzed their genetic diversity, and phylogenetic analysis was performed by comparison with Korean cattle (KC, called Hanwoo) and breeds from China and Japan. A nucleotide substitution was detected in the KB cattle, and two haplotypes were defined. In the neighbor-joining (NJ) tree, the haplotypes of KB were located in Bos taurus lineage with those of KC, Japanese black (JB), Yanbian and Zaosheng breeds. However, the haplotypes of Chinese breeds, excluding Yanbian and Zaosheng, were separated into B. taurus and B. indicus lineages. In the NJ tree of breeds based on Dxy genetic distances, Chinese breeds mixed with B. taurus and B. indicus lineages were located between B. indicus and B. taurus lineages. KB was contained within B. taurus lineage and was determined to be genetically more closely related to two Chinese (Yanbian and Zaosheng) breeds than to KC and JB. The haplotype distribution and the results of the phylogenetic analysis suggest that KB and KC have genetic differences in their mtDNA cyt b gene sequences.

• KSBB Journal
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• v.17 no.4
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• pp.370-375
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• 2002

Isolation of a gene and determination of its expression pattern are essential in understanding its function. Among the genes localized in 12ql3, stSG3435 EST was chosen to study its expression pattern. The full-length CDNA was cloned by screening of human brain CDNA library and its sequence was determined by serial deletion followed by automated sequencing of the clones with overlapping fragments. The sequence analysis revealed that stSG 3435 CDNA displayed 100% identity to human MYGI and 86% identity to mouse melanocyte proliferation gene-1 (Gamm 1) originally identified from melanocyte, suggesting that MYGI determined by Northern blot analysis revealed the strongest expression in testes with ubiquitous expression in all the tissues tested. In order to investigate the cellular localization of its protein product, the green fluorescence protein gene was fused into the full-length coding sequence of MYGI, Transfection of the fusion construct followed by confocal microscopy resulted in the green fluorescence signal as a punctate state in cytoplasm indication that MYGI was localized in one of the cellular organelles.

• Korean journal of applied entomology
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• v.35 no.4
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• pp.315-320
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• 1996

The effects of 2-arnino-3-methyIimidazo[4,5-fq]u inoline (IQ), 2-amino-6dimethyl-dipyrido[l,2-a;3',2'-d] imidazole (Glu-P-1) and aflatoxin B1 (AFBI) on the mitotic recombinations and somatic chromosome mutations were investigated using the transgenic Drosophila bearing a chimeric gene consisting of a promoter region of Drosophila actin 5C gene and rat DNA polymerase $. For investigating mitotic recombinations and the somatic chromosome mutations, the heterozygous (mwhl+) strain possessing or lacking transgene pol P was used. The spontaneous frequency of small mwh spots, due to deletion or nondisjunction etc., in the non-transgenic w strain and the transgenic plpol $1-130 strain was 0.351 and 0.606, respectively. The spontaneous frequency (0.063) of large mwh spots, arising mostly from somatic recombination between the centromere and the locus mwh, in the transgenic plpol $1-130 strain, was about three times higher than that (0.021) of the non-transgenic w strain. The mutant clone frequencies of two types induced by two heterocyclic mines (IQ and Glu-P-1) and AFBl in the transformant pbol PI-130 were two or three times higher than those in the host strain w. These mean that rat DNA polymerase P participates at least in the somatic chromosome mutations and mitotic recombination processes. And the present results suggest that the transgenic Drosophl!~ used in this study can be used as a hypersensitive, in vivo short-term assaying system for various environmental mutagens.

• Clinical and Experimental Reproductive Medicine
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• v.29 no.4
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• pp.303-310
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• 2002

Objective s: To estimate the frequency of Y chromosome microdeletions in the Korean population of infertile men and to evaluate the relationship between microdeletion on the Y chromosome and clinical phenotypes of infertile men with idiopathic azoospermia and oligozoospermia. Materials and Methods: Genomic DNA was extracted from blood samples collected from 330 infertile men attending the Infertility Clinic at Samsung Cheil Hospital, Korea. Six sequence tagged sites (STSs) spanning the azoospermia factor (AZF) regions of the Y chromosome were amplified by polymerase chain reactions (PCRs). Results: Microdeletions on Y chromosome were detected in 35 (10.6%) of the 330 infertile men. Most of the microdeletions (91.4%) involved AZFb or AZFc. The high incidence of microdeletions were found in AZFc region (57.1%), but the low in AZFa (8.6%) and AZFb (5.7%). Larger microdeletions involving two or three AZF regions were detected in 28.6% of cases. All patients (6 patients) with deletion of AZFa region showed no germ cell phenotypes, Sertoli cell only syndrome or Leydig cell hyperplasia in histopathologic examinations. Conclusion: Microdeletions on the Y chromosome, especially, at AZFc/DAZ regions may be the major cause of azoospermia and severe oligozoospermia. We suggest that idiopathic infertile men have genetic counselling and microdeletion analysis on the Y chromosome before IVF-ET and ART program.

• Clinical and Experimental Reproductive Medicine
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• v.23 no.3
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• pp.367-377
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• 1996

Genes on the long arm of Y chromosome, particularly interval 6, are believed to playa critical role in human spermatogenesis. The objective of this study was to validate a sequenced-tagged site(STS)-mapping strategy for the detection of Yq microdeletion and to use this method to determine the proportion of men with Yq microdeletions in idiopathic, obstructive, nonobstructive azoospermia, severe OATS and in normal males. We analyzed three STS markers mapped to interval 6 within long arm of the Y chromosome from 106 nonobstructive, 30 obstructive azoospermia, 15 severe OATS patients, and normal 42 males in Korean men. By PCR, we tested leukocyte DNA, for the presences of STS markers(DAZ, sY129 and sY134) and SRY gene as internal control. And PCR results were confirmed by Southern hybridization, and were investigated by SSCP analysis for DAZ gene mutation. None of 42 normal males and 30 obstructive azoospermia had microdeletions, Of the 15 severe OATS typed with DAZ, sY129 and sY134, 3(20.0%) patients failed to amplify 1 or more STS markers, and of the 106 nonobstructive azoospermia typed with DAZ, sY129 and sY134, 12(11.3%) patients failed to amplify 1 or more STS markers. From these results, high prevalence(12.4%) of Yq deletion(DAZ, sY129, sY134) in men with nonobstructive idopathic azoospermia and severe OATS were observed in Korean infertility patients. To avoid the infertile offspring by assisted reproductive technique using ICSI or ROSI, genetic diagnosis will be needed in IVF-ET program.

• Korean Journal of Medicinal Crop Science
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• v.24 no.3
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• pp.207-213
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• 2016

Background: This study aimed to investigate the flowering and fruits formation characteristics of Korean Panax ginseng varieties. The results will provide basic data for ginseng seed production and breeding program. Methods and Results: The characteristics investigated included flowering date, seed setting rate, seed type and seed production. The ginseng variety Chungsun had the earliest emergency and flowering dates, whereas Sunhyang showed late emergence and flowering dates. The emergence date of Chunpoong was not later than that of the other varieties, but the flowering date was delayed. The seed setting rate was 64.6%, 75.8%, 78.5% and 74.4% for three, four, five and six-year-old varieties, respectively. The ratio of double seed (RD) for Sunhyang and Chungsun were higher than those for the others, whereas the RD for Chunpoong was the lowest. Yunpoong and Sunone had many stems per plant and a high seed production rate. Seed production was 21.7, 67.7, 74.4 and 89.0kg/10 a in three, four, five and six-year-old varieties, respectively. Conclusions: The emergence date ranged from April 15 to 25, and the flowering date was from May 10 to the 19 for the new ginseng varieties. The average seed multiplication of the ginseng varieties was about 8.5 and 21.1 times a year for varieties in which, seed-production occurs once a year for over four years and four times over six years, respectively.

• The Korean Journal of Ecology
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• v.26 no.4
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• pp.181-188
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• 2003

Frequencies of each size class, annual change of tuber state and above ground/belowground rate in sect. Pes-gallinaceua group of Corydalis (Fumariaceae) were studied to clarify spring ephemeral´s growth characteristics from 1999 to 2000 in Namhansansung area. Shoot grew from the tuber corresponding with soil thawing in the early growth season, and the deeper tuber was located in soil, the later shoot germinated. Shoot appeared in middle March and died early in May, and the period of total growth season was 45 days. Frequencies were 40% (in total dry weight) or 53% (in tuber´ dry weight) in the smallest size class, and 93% (in total dry weight) or 96% (in tuber´ dry weight) below medium size, therefore, almost all of plants were small. Specific gravity of tuber (SGT) gradually decreased from late growth season to the time that leaf unfolded in next year. At this time, the value of SGT was 0.14. Thereafter, SGT increased to 0.42 at withering time of aboveground part. During growth season, the SGT of flowering plants was lower than that of non-flowering plants. And SGT difference between flowering plants and non-flowering plants was significant at 1% level. In the late growth season, SGT decreased along the increase of tuber volume. The tuber volume roughly increased along the leaf area but there was no a conspicuous trend. Shoot/tuber rate had nothing to do with tuber volume. SGT decreased and leaf area increased along the tuber dry weight, and tendencies of increase or decrease were conspicuous. Therefore, the smaller the plants were, the larger organic matter accumulated in tuber during a growth season. Shoot/tuber rates were various along each plant.

• Korean journal of applied entomology
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• v.2
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• pp.47-54
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• 1963

The chestnut gall wasp, Dryocosmus kuriphilus $Y^{ASUMATSU}$, has been recently distributed and chestnut trees have teen seriously damaged by this insects in Korea. However, this insect pest has not been investigated in Korea. This paper gives an account of field and Laboratory observations on the seasonal occurrence and the life history of the chestnut gall wasp, and the damage of the chestnut trees by. its insects. This study was carried out at Chungchungpukdo, Kangwon-do and Kyungi-do in Korea, from 1961 to 1963, inclusive. 1. The Chestnut gall wasp (Dryocosmus kuriphilus $Y^{ASUMATSU}$) has one generathion per year and females are only known. 2. The adult wasps were observed from late June to late July with peak of wasp emergence about early and middle July. Most of new adult wasps were coming out from, the galls in the morning rather than the afternoon. 3. Each wasp produced auerage 198.5 eggs and about 4.89 eggs was observed in each bud of the chestnut tree. 4, The egg period of wasps was about 30 days and they were hatching from July to August. 5. The larval period of wasps were from middle of August to middle of next June and pupation took places about early June to middle of July. 6. The adult wasps were ovipositing into the buds and from early May next year, galls were growing. They caused stunting of growth and no fruiting or dying of the chestnut tries. 7. The first observation of the wasp was made at Chaechun of Chungchung-pukdo in 1958. Up to 1963 this insects are distributed in Kangwon-do, Kyunggi-do, north part of Chungchung-pukdo and north part of Kyungsangpukdo