• Journal of Food Hygiene and Safety
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• v.34 no.1
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• pp.94-99
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• 2019

The objective of this study is to establish the shelf life of non-pasteurized whole egg, egg yolk and egg white liquid. Each sample was stored for two weeks at $5^{\circ}C$, $10^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$, and then sensory, microbial, and physicochemical tests were performed periodically. The estimation of shelf life was based on the microbial standards of total viable counts and coliforms. The chemical properties highly correlated with the sensory evaluation were also used. Our results showed that the shelf life was the most influenced by microbial properties. Exceptionally, however, whole egg and white liquid stored at $5^{\circ}C$ and $10^{\circ}C$ with limited bacterial growth were affected by chemical property. The shelf life of the three non-pasteurized liquids was calculated to be less than one day at over $15^{\circ}C$. At $5^{\circ}C$ and $10^{\circ}C$, the shelf life was calculated to be 5 d and 1 d for egg yolk liquid, 5 d and 5 d for egg white, and 7 d and 5 d for whole egg, respectively. Therefore, it is advisable to establish reasonable shelf life in the more specific manner based on consideration of these findings.

• Research in Plant Disease
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• v.19 no.1
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• pp.36-44
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• 2013

Carnation is considered to be one of the top three cutting flowers in the world, which is a main crop with 21 billion annual volume of manufacture. The four carnation items such as cuttings, seed, plant and unrooted cuttings are imported and exported. Viruses can be easily transmitted during vegetative propagation of carnation. Carnation necrotic fleck virus (CNFV) and Carnation ringspot virus (CRSV) are designated as Korea plant quarantine viruses and inspected. This study was aimed to develop specific primer sets for easy and rapid detection of CNFV and CRSV. Two RT-PCR primer sets were efficiently amplified 288 and 447 bp fragments for CNFV and 503 549 bp fragments for CRSV. Furthermore, developed nested primer sets make possible to high sensitive detection and verification. CNFV nested PCR primer sets all produced band of 147 bp and CRSV nested PCR primer sets did bands of 395 and 347 bp. In addition, plasmid inserted 6 sequences in amplicon were used as a positive control to improve inspection confidence. The successful application of PCR module newly developed in this study will be highly useful for detect of CNFV and CRSV for quarantine inspections.

• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.335-339
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• 2014

Cowper chlorotic mottle virus (CCMV) is the 'controlled' quarantine virus as plant pathogenic virus that are classed as group VI (+) ssRNA virus that belongs to the genus Bromovirus and family Bromoviridae, When plants that are Phaseolus vulgaris, Clitoria ternatea, Nicotiana tabaccum, Glycine max, Vigna unguiculata and Vigna siensis, and Arachis hypogaea is imported in domestic. In this study, inspection system is implemented to analyze CCMV accurately and rapidly by developing RT-PCR, nested PCR, and gene insertion positive control. It is expected that the method developed in this study will contribute to the plant quarantine to be consistently utilized in the field.

• Korean Journal of Agricultural Science
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• v.40 no.4
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• pp.281-287
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• 2013

In Korea, isolated cultivation has been implemented for 102 genera, including about 250 species, each of which has underwent microscopic inspection, cultivation of bacteria in selective medium, analysis of physiology and biochemistry, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). The number of isolated microorganisms was 8,307 in the period of 2005-2012, and bulbs and tubers had the greatest diversity of microorganisms, of 5,165 (62.2%), followed by 2,119 (25.0%) sapling, 796 (9.6%) seed, 150 (1.8%) cutting slip, 70 (0.8%) branch graft and 7 (0.1%). The number of cases which were disqualified were 413 (4.97%), after the detection of 47 disease causing species of microorganism. Viruses predominated, with 27 species, followed by 16 fungi, a viroid, a Chromalveolata and 2 further species. Top on the list of detection was Arabis mosaic virus (77 cases), followed by Tobacco rattle virus (70 cases), Lily symptomless virus (46 cases) and Penicillium expansum (46 cases).

• Journal of Veterinary Clinics
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• v.27 no.4
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• pp.421-426
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• 2010

In view of free from bluetongue (BT) in the domestic cattle population in Korea, the key of quarantine testing for BT virus (BTV) infection is detection of cattle previously exposed to the virus. The objective of this study was to estimate the probability of detecting a cattle infected with BTV using a stochastic modeling analysis of existing quarantine testing data. Three testing scenarios were considered in this study: serological testing of all animals in all imported lots (scenario 1), serological testing of a sample of cattle from all imported lots (scenario 2), and serological testing of 50% of imported lots (scenario 3). In scenario 2 and 3, it was assumed that cattle were sampled (sample size) within each lot to detect 5% of the cattle in each lot with a 95% confidence, taking into account diagnostic sensitivity of the ELISA (enzyme-linked immunosorbent assay). The model output was the total number of BTV-infected cattle and the prevalence of BTV infection in imported cattle from the US, Australia, Canada and Japan. Compared to the scenario 1, the probability of detecting a BTV-infected cattle was estimated to be 19% and 1.6% in scenario 2 and 3, respectively. Furthermore, the analyses showed a 95% confidence that BTV prevalence was less or equal to $9.7{\times}10^{-4}$ (median = $1.5{\times}10^{-5}$), indicating that, for the scenario 2 and 3 with serological testing for a sample of cattle, the risk of introducing an exotic strain of BTV into Korea through the importation of live cattle would not be acceptable.

• Korean journal of applied entomology
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• v.58 no.1
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• pp.15-25
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• 2019

Mites in the family Parasitidae (Mesostigmata) are important predators in soil ecosystem. During the soil acarine biodiversity study from diverse habitats in the Republic of Korea, we found newly recording species of parasitid mites of genus Cycetogamasus and Neogamasus. This paper reports two species Cycetogamasus coreanus and C. corculatus of genus Cycetogamasus and five species Neogamasus eogenualis, N. tikhomirovi, N. laciniatus, Neogamasus kengicus and Neogamasus mahunkai of genus Neogamasus as new record in the Republic of Korea.

• Journal of Apiculture
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• v.32 no.2
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• pp.99-109
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• 2017

The multiple real-time PCRs against pathogens of Bombus species including DWV, IAPV, KBV, SBV, BQCV, kSBV, SBPV and Paenibacillus larvae, Mellisococcus plutonius, Lysinibacillus fusiformis, and Klebsiella oxytoca have been developed. One extracted nucleic acid from Beesample could be applied to 11 different PCRs in same time and condition. Specific PCR-products were amplified qualitative and quantitative manner inner 20 minutes successfully, when each 1000 molecules of pathogen-specific target DNA is existed as template, respectively. The multiple PCR detection that we propose would be expected to apply to quarantine test for international exchange of Bombus species.

• KOREAN POULTRY JOURNAL
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• v.39 no.1 s.447
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• pp.94-96
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• 2007

본고에서는 AI 종식이후 농가들이 재입식 절차요령에 대한 궁금증을 풀어주고자「가축질병 위기대응 실무메뉴얼」에 실린 사후관리와 재입식 요령을 바탕으로 정리하여 게재하였다. HPAI가 발생한 위험지역 내에서 다시 재입식을 하기 위해서는 최소한 3개월 정도가 소요된다. 즉, AI의 최대 잠복기가 3주이기 때문에 그동안 추가 발생이 없으면 위험지역은 경계지역으로 전환되고, 이후 1달간의 상황을 지켜본 후 이상이 없으면 이동제한 조치가 추가로 해제된다. 이동제한 조치가 풀렸다고 해서 AI발생지역에서 재입식이 가능한 것은 아니다. 이동제한 조치가 풀린뒤 21일간의 입식시험을 거쳐야 하고 시험사육뒤 검역원에 21일간의 정밀검사를 받은 후에 비로소 입식을 할 수 있다. 이 모든 것은 추가 발생이 되지 않는다는 전제하에 이루어진다.

• KOREAN POULTRY JOURNAL
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• v.43 no.12
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• pp.136-137
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• 2011

가금질병연구회(회장 송창선)가 지난 2일 천안상록리조트에서 150여명의 회원 및 농가들이 참석한 가운데 개최되었다. 가금질병연구회는 매년 두 차례 양계질병을 주제로 세미나를 개최하고 있다. 금년에는 아쉽게도 AI와 구제역 발생으로 전반기에 열리지 못했고 이번에 개최하게 되었다. 가금질병연구회는 신진 양계수의사들은 물론 원로 수의사들까지 함께 모여 최근에 문제가 되고 있는 질병에 대해 발표하고 서로 토론하는 자리를 갖고 있을 정도로 폭넓은 활동을 벌이고 있다. 농림수산검역검사본부와 한국양계수의사회가 함께 주관을 하고 있는 가금질병연구회 세미나는 명실 공히 양계질병 분야를 이끌어가는 중심축으로 자리잡아가고 있다. 이날 세미나는 오경록(남덕쎄니텍) 박사와 김선중(바이오포아) 박사가 좌장을 맡아 진행했으며 세균성 질병으로는 살모넬라와 장염, 바이러스성 질병으로는 고병원성조류인플루엔자(HPAI)와 뉴모바이러스에 대한 발표가 있었다.

• 동물약계
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• no.92
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• pp.2-5
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• 2003

[ $\cdot$ ]동물용의약품등 제조업 신규 허가 $\cdot$동물용의료용구 제조업 신규 허가 $\cdot$동물용의약품등 수입관리자 신규 승인 $\cdot$동물용의약품 제조$\cdot$수입업 폐업 신고 $\cdot$동물용의약품 제조$\cdot$수입업 휴업 신고 $\cdot$동물용의약품등 제조업 허가사항 변경 $\cdot$동물용의약품등 수입자 확인사항 변경 $\cdot$동물용의약품 품질관리우수업체 신규 지정 $\cdot$동물용의약품 품질관리우수업체 변경 지정 $\cdot$구제역방역실시요령 제정 고시 $\cdot$혈청검사및검역수수료규칙 개정(안) 입법예고 $\cdot$소독설비의운영및소독요령(안) 의견 조회 $\cdot$소독약품 효력시험 관련 사항 $\cdot$품질관리우수업체 지정신청 촉구 $\cdot$닭$\cdot$오리 농가 출입차량 세부관리요령 시달 $\cdot$미국산 반추동물 및 유래 생산물 수입금지 $\cdot$동물용의약품 GMP 적용확대에 따른 조치