• Korean Journal of Environment and Ecology
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• v.23 no.5
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• pp.392-410
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• 2009

The vascular plants recorded from the 11 sites of coastal sand dune in North Gyeongsang Province are as follows: 204 taxa, 2 hybrids, 1 form, 22 varieties, 1 subspecies, 178 species, 132 genera, 43 families. These areas also present rare plant (Glehnia littoralis); 20 taxa, 20 species, 20 genera, and 15 families of floristics special plants; 46 taxa, 2 variety, 44 species, 30 genera, and 12 families of naturalized plants. The ratio of urbanization and naturalized index is 16.14% and 22.55% respectively. Life form spectrum is Th-$R_5-D_4$-e type. The number of species recorded from the coastal sand dune in Hujeong area turned out to be the highest (44.6%), and the coastal sand dune in Heseo area showed the highest naturalized index (31.58%).

• Journal of The Korean Society of Clinical Toxicology
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• v.15 no.2
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• pp.107-115
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• 2017

Purpose: Glehnia littoralis has been used to treat ischemic stroke, phlegm, cough, systemic paralysis, antipyretics and neuralgia. The pharmacological mechanisms of Glehnia littoralis include calcium channel block, coumarin derivatives, anticoagulation, anti-convulsive effect, as well as anti-oxidant and anti-inflammatory effects. Alpha-amanitin (${\alpha}$-amanitin) is a major toxin from extremely poisonous Amanita fungi. Oxidative stress, which may contribute to severe hepatotoxicity was induced by ${\alpha}$-amanitin. The aim of this study was to investigate whether Glehnia littoralis ethyl acetate extract (GLEA) has the protective antioxidant effects on ${\alpha}$-amanitin -induced hepatotoxicity. Methods: Human hepatoma cell line HepG2 cells were pretreated in the presence or absence of GLEA (50, 100 and $200{\mu}g/ml$) for 4 hours, then exposed to $60{\mu}mol/L$ of${\alpha}$-amanitin for an additional 4 hours. Cell viability was evaluated using the MTT method. AST, ALT, and LDH production in a culture medium and intracellular MDA, GSH, and SOD levels were determined. Results: GLEA (50, 100 and $200{\mu}g/ml$) significantly increased the relative cell viability by 7.11, 9.87, and 14.39%, respectively, and reduced the level of ALT by 10.39%, 34.27%, and 52.14%, AST by 9.89%, 15.16%, and 32.84%, as well as LDH by 15.86%, 22.98%, and 24.32% in culture medium, respectively. GLEA could also remarkably decrease the level of MDA and increase the content of GSH and SOD in the HepG2 cells. Conclusion: In the in vitro model, Glehnia littoralis was effective in limiting hepatic injury after ${\alpha}$-amanitin poisoning. Its antioxidant effect is attenuated by antidotal therapy.

• Journal of The Korean Society of Clinical Toxicology
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• v.16 no.2
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• pp.108-115
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• 2018

Purpose: Glehnia littoralis has been reported to have several pharmacological properties but no in vivo reports describing the protective effects of this plant on${\alpha}$-amanitin-induced hepatotoxicity have been published. ${\alpha}$-Amanitin is a peptide found in several mushroom species that accounts for the majority of severe mushroom poisonings leading to severe hepatonecrosis. In our previous in vitro study, we found that ${\alpha}$-amanitin induced oxidative stress, which may contribute to its severe hepatotoxicity. The aim of this study was to investigate whether Glehnia littoralis acetate extract (GLEA) has protective antioxidant effects on ${\alpha}$-amanitin-induced hepatotoxicity in a murine model. Methods: Swiss mice (n=40 in all groups) were divided into four groups (n=10/group). Three hours after giving ${\alpha}$-amanitin (0.6 mg/kg, i.p.) to the mice, they were administered silibinin (50 mg/kg/d, i.p.) or Glehnia littoralis ethyl acetate extract (100 mg/kg/d, oral) therapies once a day for 3 days. After 72 hours of treatment, each subject was killed, cardiac blood was aspirated for hepatic aminotransferase measurement, and liver specimens were harvested to evaluate the extent of hepatonecrosis. The degree of hepatonecrosis was assessed by a pathologist blinded to the treatment group and divided into 4 categories according to the grade of hepatonecrosis. Results: GLEA significantly improved the beneficial functional parameters in ${\alpha}$-amanitin-induced hepatotoxicity. In the histopathological evaluation, the toxicity that was generated with ${\alpha}$-amanitin was significantly reduced by GLEA, showing a possible hepatoprotective effect. Conclusion: In this murine model, Glehnia littoralis was effective in limiting hepatic injury after ${\alpha}$-amanitin poisoning. Increases of aminotransferases and degrees of hepatonecrosis were attenuated by this antidotal therapy.

• Korean Journal of Environment and Ecology
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• v.23 no.4
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• pp.346-352
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• 2009

This study was conducted in order to provide as a basic materials of sea cliff vegetation structure through distribution related with the landform and growth pattern of mixed growth plants by ordination method in the Peucedanum japonicum community of Korea. The mixed growth plants of frequency over 10% growth with Peucedanum japonicum community of sea cliff was investigated as a total 26 species, and the frequency of appearing plants was the highest in the Aster spathulifolius(55.7%). Growing pattern of mixed growth plants was a difference by the landform factors as such the inland side, coastline side, flat and slope. Growing pattern was classified into typicality type, one-sided type and complex type. Growing pattern of the typicality type was good distribution in total landform factors, the one-sided type was good distribution in 1 landform factor and the complex type was good distribution in 2 landform factors. In growing pattern of mixed growth plants with Peucedanum japonicum, Artemisia capillaris and Sedum oryzifolium was typicality type, Elymus dahuricus and Artemisia princeps was one-sided type, and Aster spathulifolius, Miscanthus sinensis, Calystegia soldanella and Lysimachia mauritiana was complex type. In total plants of mixed growth, growing pattern of 16 species of 61.5% was good as complex type.