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Seasonal Variations of Nitrifying Bacteria in Agricultural Reservoir (농업용 저수지에서의 질화세균의 계절적인 변화)

  • Lee, Hee-Soon;Lee, Young-Ok
    • Korean Journal of Ecology and Environment
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    • v.35 no.3 s.99
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    • pp.152-159
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    • 2002
  • The seasonal variations of nitrifying bacterial population sampled from 3 sites in Moon-Chon reservoir were analyzed by in situ hybridization with fluorescently labeled rRNA-targeted oligonucleotide probes from August 2000 until July 2001. In addition, physico-chemical parameters such as temperature, pH, chi-a and DOC were measured to determine correlations between those factors and the size of nitrifying bacterial populations. Total bacterial numbers varied in the range of $0.8{\sim}1.5{\times}10^6\;cells/ml$ independent of sites and had the maximal values in March at all 3 stations. The ratio of eubacteria to total bacteria ranged from 44.9% to 79.5%, and the ratio of each nitrifying bacteria to eubacterial numbers reached only $1.0{\sim}7.4%$. The variations of ammonia-oxidizing bacteria ranged from $1.1{\times}10^4$ to $3.0{\times}10^4\;cells/ml$ without noticeable peak values whereas those of nitrite-oxidizing bacteria varied in $1.3{\sim}5.7{\times}10^4\;cells/ml$ with the increasing tendency in winter regardless of the sites. Moreover it was observed that the numbers of nitrite-oxidizing bacteria were higher than those of ammonia-oxidizing bacteria. Total bacterial numbers correlated with water temperature (r = 0.355, p<0.05) and DOC (r = 0.58G, p<0.01) positively whereas nitrite-oxidizing bacteria correlated with temperature (r = -0.416, p<0.05) and pH (r = -0.568, p = 0.001) negatively. In addition, DOC represented good correlations with eubacterial numbers (r = 0.448, p<0.01). These results indicate that temperature, DOC and pH might be one of the main factors affecting variations of bacterial populations in the aquatic ecosystem. It was also suggested that FISH method is a useful tool for detection of slow growing nitrifying bacteria.

Reduction in bitter taste and quality characteristics in pickled bitter melon (Momordica charantia L.) by different pretreatment conditions (전처리 조건에 따른 여주(Momordica charantia L.) 초절임의 쓴맛 감소와 품질평가)

  • Park, HyoSun;Moon, BoKyung;Kim, Suna
    • Korean Journal of Food Science and Technology
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    • v.48 no.5
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    • pp.466-473
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    • 2016
  • This study was performed to investigate the reduction in bitter taste and quality characteristics by pretreatments (brining; 1, 5% and blanching; 1, 3 min) in pickled bitter melon, respectively. We prepared picked bitter melon samples at 1%-1 min, 1%-3 min, 5%-1 min, 5%-3 min. Total polyphenol and total flavonoid contents were found to be the highest in 5%-1 min at $14.23{\pm}0.40mg\;CE/g$ (dry) and $4.46{\pm}0.10mg\;RE/g$ (dry), respectively. L-ascorbic acid level was the highest in control samples. Arginine and glutamic acid were increased by brining and blanching. ABTS and DPPH radical scavenging activity were found to be the highest at $43.60{\pm}0.40$ and $44.88{\pm}0.20%$ at 5%-1 min, respectively. ${\alpha}-glucosidase$ inhibitory activity was the highest at 5%-1 min. The a value was statistically different, whereas L and b values were similar among different pretreatments. Hardness in pretreated samples was decreased as compared to that in the control. Among sensory evaluations, 'color' did not indicate any statistical difference, while 'texture', 'bitterness preference' and 'overall preference' increased with pretreatments, and 'bitter intensity' decreased.

Utilization Level and Associated Factors of Complementary and Alternative Medicine in the Older Population before Death (사망 전 노인들의 보완대체요법 이용수준 및 관련 요인)

  • Cho, Nam-Hong;Woo, Eun-Kyung;Yi, Jee-Joen;Yi, Sang-Wook;Cho, Hang-Sok;Kim, Sun-Hyun
    • Journal of Hospice and Palliative Care
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    • v.7 no.1
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    • pp.37-48
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    • 2004
  • Purpose: Population using complementary and alternative medicine (CAM) and the cost or it are increasing all over the world. In this study, we investigated the utilization state of CAM in the elderly people during the last 6 months of life. Methods: We found 4,210 persons in Seoul, older than 65 years who received a funeral subsidy from health insurance after death during the latter half of the year, 2001. We stratified them by age and gender and selected 301 persons and surveyed them by questionnaire. Results: 247 persons (83.1%) used at least one of CAM during their last 6 months of life. Diet and Nutrition remedy showed the highest utilatization rate (65.5%), and drug therapies also were much used (29.9%). Utilization rate was higher in female (60%) and according to the cause of death, the highest in a geriatric disease groups, and the lowest in the cancer group. Whole satisfaction to CAM was high with average 4.25 points out of total 6, and adverse effects were almost free with average 5.55 points out of total 6. Satisfaction was the highest in Diet and Nutrition remedy but the lowest in Manipulative and Body-Based methods. Adverse-effect score was best in Manipulative and Body-Based methods. According to age and the causative disease of death, there were significant differences in satisfaction and adverse effect levels. Conclusion: Majority of old people used at least one of CAM during their last 6months of life. Whole satisfaction and adverse-effect level were very excellent.

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A Study on the Marketing System of Walnut -With Special Reference to the Case Survey in Cheonwongun Districts- (호도의 유통체계(流通體系)에 관(關)한 연구(硏究) -천원군(天原郡)의 사례조사(事例調査)를 중심(中心)으로-)

  • Jeon, Sang-Don;Cho, Eung-Hyouk
    • Journal of Korean Society of Forest Science
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    • v.79 no.2
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    • pp.187-195
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    • 1990
  • The following conclusions have been obtained with special reference to the walnut marketing system in Cheonwongun districts 1. The marketing channel of walnut in the producing areas was mainly depended on the individual selling by 89.58%. and sale through farmer's coops and forest owner's association by 10.42%, and share of walnut through fatmer's coops was 84.58%. 2. The market structure in assembling stage of walnut can be represented as oligopoly considering the market share of 86.26% derived by CR3 method. 3. Direct selling from producers to consumers would be recommendable to reduce marketing margin considering the 77.20% of sale's dependency on assembler-commisioner. 4. Two major reasons to follow the marketing channel of assembler-commissioner were the convieniency (45.00%) and dealing with small quantity of walnut (20.00%). Let the walnut producers follow the institutional marketing channels such as farmer's coops and forest owner s association, special actions including better conveniency, smaller quantity and the procedures should be improved. 5. Farmer's share of walnut was estimated as 54.93% and total marketing margin was 45.0% of which 36.70% destined to the retail stage. 6. The price index in November was the lowest(83.63) due to the flood and hunger sale and the index in April was the highest(115.74). To cope with the severe price fluctuation and to stabilize seasonal walnut price, sale's in advance, credit supply and provision of storage facilities must be considered in policy-making decision for forest farmers.

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Contents analyses of teaching·learning research on housing education of home economics for secondary schools (중등학교 주생활교육 교수·학습 개발연구 내용분석)

  • Joo, Hyunjung;Cho, Jaesoon;Choi, Yoori
    • Journal of Korean Home Economics Education Association
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    • v.29 no.3
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    • pp.33-48
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    • 2017
  • The purpose of this research was to analyze the contents of housing teaching learning studies in Home Economics of secondary schools since 2001. The 22 research, drawn from the database 'riss4u', were analyzed in terms of general information of the paper (studied institution & year, implementation & evaluation, subject of study & size) and specific contents of teaching learning plans (theme, curricula & textbooks, methode & # of lessons, resources). The results showed that most studies were reported during the 7th or the 2007 revised curricula period. All, except one doctoral dissertation, were master's theses from a few universities. In all studies, ranging from 2 to 15 lessons, teaching learning plans were implemented and evaluated in the class of the researcher while some were applied in other schools, too. The theme of the teaching learning plans varied but were concentrated on one out of two content elements and two out of six learning elements. The 2007 revised curriculum seems to be an important turning point, not only reinforcing the analyses of the curricular and textbooks in the analyzing stage but also facilitating the use of various methods for the lessons in the developing stage. Practical problem based model was the most frequently adopted, while cooperative learning and ICT served as fundamental although not always mentioned. Various teaching resources such as UCC, reading materials, PPT were developed for the teacher. Activity sheets were the most frequently used for the students, followed by reading materials. Because teaching learning is an essential core of education, teaching learning studies should be more actively conducted and the variety of subject topics, methods and resources should also be obtained by more researchers.

Meta-analysis of Intravitreal Injection of Anti-vascular Endothelial Growth Factors for Diabetic Macular Edema (당뇨황반부종에서 항혈관성장인자의 유효성과 안전성: 네트워크 메타분석)

  • Tchoe, Hajin;Shin, Sang Jin;Suh, Jae Kyung;Cho, Songhee;Yang, Jangmi;Kang, Min Joo;Jee, Donghyun
    • Journal of The Korean Ophthalmological Society
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    • v.60 no.2
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    • pp.144-151
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    • 2019
  • Purpose: Intravitreal aflibercept, ranibizumab, bevacizumab, and dexamethasone are the most widely used drugs in the treatment of diabetic macular edema (DME). The aim of this study was to compare the efficacy and safety of anti-vascular endothelial growth factors and dexamethasone for the treatment of DME. Methods: There were nine previous systematic reviews on this topic; we updated these high-quality reviews. Seven studies were added to two studies following a literature search. Efficacy outcomes were 1) average improvement in visual acuity, 2) proportion of patients who experienced an improvement in vision (an increase in best-corrected visual acuity (BCVA) of ${\geq}15$ in the Early Treatment Diabetic Retinopathy Study [ETDRS]), and 3) proportion of patients who experienced worsening vision (a decrease in BCVA of ${\geq}15$ in the ETDRS). Safety outcomes included systemic adverse events and ocular-related adverse events. Results: The mean difference in the BCVA for ranibizumab versus bevacizumab treatment was 0.16 (95% confidence interval [CI]: -0.02, 0.34), and that for ranibizumab versus aflibercept was -0.08 (95% CI: -0.26, 0.10). The mean difference in the change of BCVA for aflibercept versus ranibizumab was -0.20 (95% CI: -0.40, -0.01), and that for aflibercept versus bevacizumab was -0.34 (95% CI: -0.53, -0.14). Other efficacy outcomes showed similar trends, and there was no significant difference between treatments. There was also no significant difference in both systemic and ocular adverse events rates between the treatments. Conclusions: In DME patients, the efficacy of aflibercept was found to be higher with respect to BCVA changes compared with ranibizumab or bevacizumab. However, there were no significant difference in terms of visual acuity improvement or visual acuity of more than 15 letters, nor in terms of anti-vascular endothelial growth factors (as a safety outcome).

Development and Preliminary Evaluation of a Leukocyte Removal Aptamer Filter (압타머를 이용한 백혈구제거필터의 개발 및 예비평가)

  • Lee, Yangwon;Jung, Eun-Suk;Choi, Kyoung Young;Kim, Myung Han;Kwon, So Yong;Cho, Nam Sun;Kim, Jin Sook;Park, Han Jeong;Han, Byoung Don;Yoon, Soo-Young
    • The Korean Journal of Blood Transfusion
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    • v.23 no.2
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    • pp.107-114
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    • 2012
  • Background: Leukocyte reduction filters are widely used to prevent transfusion reactions caused by leukocytes in blood components. Commercial filters are not sufficient for removal of leukocytes for prevention of transfusion associated graft-versus-host disease; therefore, irradiation of blood components was performed using expensive equipment. Techniques using an aptamer substituted for antibody have been developed and are available in clinical areas. The purpose of this study is to develop the aptamer filter system and to evaluate its efficiency and the possibility of its clinical application. Methods: Aptamers targeted to CD45 were selected by the Postech Aptamer Initiative. The aptamer filter in which aptamers attached to beads were bound to leukocytes and removed by magnetic field was developed. Filtration of 14 units of leukoreduction-red blood provided by Korean Red Cross Blood Services was performed using aptamer filters. Leukocyte removal rate and red cell recovery rate were evaluated and bacterial culture was performed. Results: After filtration using the aptamer filters, 45.6% of leukocytes were additionally removed and the red cell recovery rate was 92.8%. No growth in the bacterial culture was observed. Conclusion: In order to apply the cell depletion technique utilizing an aptamer to blood filter system, we developed and evaluated the aptamer filter system. Through improvement of the binding efficiency of the aptamer and the filtering process, and application of the various aptamers for other different cells, we suggest that this technique can be applied in the clinical area, such as a substitution for the irradiation process for TAGVHD prevention.

Prelaunch Study of Validation for the Geostationary Ocean Color Imager (GOCI) (정지궤도 해색탑재체(GOCI) 자료 검정을 위한 사전연구)

  • Ryu, Joo-Hyung;Moon, Jeong-Eon;Son, Young-Baek;Cho, Seong-Ick;Min, Jee-Eun;Yang, Chan-Su;Ahn, Yu-Hwan;Shim, Jae-Seol
    • Korean Journal of Remote Sensing
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    • v.26 no.2
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    • pp.251-262
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    • 2010
  • In order to provide quantitative control of the standard products of Geostationary Ocean Color Imager (GOCI), on-board radiometric correction, atmospheric correction, and bio-optical algorithm are obtained continuously by comprehensive and consistent calibration and validation procedures. The calibration/validation for radiometric, atmospheric, and bio-optical data of GOCI uses temperature, salinity, ocean optics, fluorescence, and turbidity data sets from buoy and platform systems, and periodic oceanic environmental data. For calibration and validation of GOCI, we compared radiometric data between in-situ measurement and HyperSAS data installed in the Ieodo ocean research station, and between HyperSAS and SeaWiFS radiance. HyperSAS data were slightly different in in-situ radiance and irradiance, but they did not have spectral shift in absorption bands. Although all radiance bands measured between HyperSAS and SeaWiFS had an average 25% error, the 11% absolute error was relatively lower when atmospheric correction bands were omitted. This error is related to the SeaWiFS standard atmospheric correction process. We have to consider and improve this error rate for calibration and validation of GOCI. A reference target site around Dokdo Island was used for studying calibration and validation of GOCI. In-situ ocean- and bio-optical data were collected during August and October, 2009. Reflectance spectra around Dokdo Island showed optical characteristic of Case-1 Water. Absorption spectra of chlorophyll, suspended matter, and dissolved organic matter also showed their spectral characteristics. MODIS Aqua-derived chlorophyll-a concentration was well correlated with in-situ fluorometer value, which installed in Dokdo buoy. As we strive to solv the problems of radiometric, atmospheric, and bio-optical correction, it is important to be able to progress and improve the future quality of calibration and validation of GOCI.

Estimation of Precipitable Water from the GMS-5 Split Window Data (GMS-5 Split Window 자료를 이용한 가강수량 산출)

  • 손승희;정효상;김금란;이정환
    • Korean Journal of Remote Sensing
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    • v.14 no.1
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    • pp.53-68
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    • 1998
  • Observation of hydrometeors' behavior in the atmosphere is important to understand weather and climate. By conventional observations, we can get the distribution of water vapor at limited number of points on the earth. In this study, the precipitable water has been estimated from the split window channel data on GMS-5 based upon the technique developed by Chesters et al.(1983). To retrieve the precipitable water, water vapor absorption parameter depending on filter function of sensor has been derived using the regression analysis between the split window channel data and the radiosonde data observed at Osan, Pohang, Kwangiu and Cheju staions for 4 months. The air temperature of 700 hPa from the Global Spectral Model of Korea Meteorological Administration (GSM/KMA) has been used as mean air temperature for single layer radiation model. The retrieved precipitable water for the period from August 1996 through December 1996 are compared to radiosonde data. It is shown that the root mean square differences between radiosonde observations and the GMS-5 retrievals range from 0.65 g/$cm^2$ to 1.09 g/$cm^2$ with correlation coefficient of 0.46 on hourly basis. The monthly distribution of precipitable water from GMS-5 shows almost good representation in large scale. Precipitable water is produced 4 times a day at Korea Meteorological Administration in the form of grid point data with 0.5 degree lat./lon. resolution. The data can be used in the objective analysis for numerical weather prediction and to increase the accuracy of humidity analysis especially under clear sky condition. And also, the data is a useful complement to existing data set for climatological research. But it is necessary to get higher correlation between radiosonde observations and the GMS-5 retrievals for operational applications.

Analysis of weighted usable area and estimation of optimum environmental flow based on growth stages of target species for improving fish habitat in regulated and non-regulated rivers (조절 및 비조절 하천의 어류 서식처 개선을 위한 성장 단계별 가중가용면적 분석 및 최적 환경생태유량 산정)

  • Jung, Sanghwa;Ji, Un;Kim, Kyu-ho;Jang, Eun-kyung
    • Journal of Korea Water Resources Association
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    • v.52 no.spc2
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    • pp.811-822
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    • 2019
  • Environmental flows in the downstream sections of Yongdam Dam, Wonju Stream Dam, and Hongcheon River were estimated with selected target fish species such as Nigra for the site of Yongdam Dam, Splendidus for the site of Wonju Stream Dam, and Signifer for the site of Hongcheon River by considering endangered and domestic species. Physical habitat analysis was performed to estimate environmental flows for the study sites by applying the Physical Habitat Simulation (PHABSIM) and RIVER2D which combined hydraulic and habitat models. Based on the monitored data for ecological environment, the Habitat Suitability Index (HSI) for the target species was estimated by applying the Instream Flow and Aquatic Systems Group (IFASG). In particular, based on the result of fish monitoring, the HSI for each stage of the growth for target species was analyzed. As a result, the Weighted Usable Area (WUA) was maximized at $4.9m^3/s$ of flow discharge during spawning, $5.8m^3/s$ during the period of juvenile, and $8.9m^3/s$ during the adult fish season at the downstream section of Yongdam Dam. The result of the Wonju Stream Dam showed an optimal environmental flow of $0.4m^3/s$, $1.0m^3/s$, and $1.5m^3/s$ during the period of spawning, juvenile, and adult. The habitat analysis for the site of Hongcheon River, which is a non-regulated stream, produced an optimum environmental flow of $5m^3/s$ in the spawning period, $4m^3/s$ in the juvenile stage and $6m^3/s$ in the adult stage.