• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.89-95
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• 2010

Dihydroflavonol 4-reductase (DFR) is a key enzyme of the flavonoid biosynthesis pathway which catalyses the NADPH-dependent reduction of 2R,3R-trans-dihydroflavonols to leucoanthocyanidins. In this study we describe cloning and expression of the genes encoding the flavonoid-biosynthetic enzyme DFR in Gypsophila paniculata L. Inspection of the 1279 bp long sequence revealed an open reading frame 1063 bp, including a 36 bp 5' leader region and 181 bp 3' untranslated region. Comparison of the coding region of this DFR cDNA sequence including the sequences of Arabidopsis thaliana, Citrus sinensis, Dianthus caryophyllus, Ipomoea batatas, Matthiola incana, Nierembergia sp, Petunia hybrida, Solanum tuberosum, Vitis vinifera reveals an identity higher than 69% at the nucleotide level. The function of this nucleotide sequences was verified by comparison with amino acid sequences of the amino-terminus and tryptic peptides from purified plant enzyme, by northern blotting with mRNA from wild type and mutant plants, by in vitro expression yielding and enzymatically active reductase, as indicated by the small leucopelargonidin peak. Genomic southern blot analysis showed the presence of only one gene for DFR in Gypsophila paniculata.

• Journal of Plant Biology
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• v.4 no.1
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• pp.1-7
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• 1961

Harn, Chang Yawl (Chonpuk U., Iri, Korea)-Studies on the interspecific crossing of FORSYTHIA genus. Kor. Jour. Bot. 4(1)1~8 1961: Interspecific crossing of two species, F. saxatilis and F. Koreana, was carried out in order to make clear the segregation ratio of style length, mode of fertility, the fertility of F1 generation, dioecism, and other taxonomic question, the result of which being summarized as follows: 1) Style length is segregated into 1:1 ratio. 2) The behaviro of fertility in the legitimate and illegitimate unions between the different species is exactly like that in the two dimorphic forms of the same species. 3) The mode of fertility between the long and short style of the F1 generation also follows that of the heterostyle plants. 4) No difficulties or irregularities are observed in the interspecific crossing and the F1's fertility. 5) In F1 generation exceedingly high morphological and physiological variations are observed. 6) The short style individual is well fertilized and sets seed when legitimately combined. The insistence that the short style is male, this genus being dioecious, is groundless. 7) Among F1 individuals, are observed a few dwarf-types with tiny and weak vegetative and reproductive organs. 8) The two species used behave in many ways like the different styles of the same species.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.26-35
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• 2002

Abeliophyllum distichum Nakai (O1eaceae) is a monotype of Korea and is distinguished from related genus Forsythia Vahl by the morphological characters such as fruit shape, flower color and etc. Even though several intraspecific taxa were reported according to the color of flowers and shape of fruits, there have been many controversals on the taxonomic indentity and status of rank on those taxa. In the present study, we performed the RAPD analysis to delimit the infraspecific taxa of Abeliophyllum distichum and to investigate the genetic polymorphism and relationships among 12 populations. 212 scorable RAPD markers with 70 common markers were found from the PCR reactions with 16 random oligoprimers and were analyzed by Nei's genetic distance. From 0.108 to 0.321 of genetic variations were showed among the taxa. Some regional groups instead of same taxa were clustered from the phonogram of UPGMA analysis. Also, we could not find distinct lineage among intraspecific taxa. The result from RAPD analysis supported that the infraspecific taxa of Abeliophyllum distichum might be the individual variations and treated as the same taxa. RAPD analysis was very useful to confirm the high gene pool with diverse genetic polymorphism among Abeliophyllum distichum populations.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.6-6
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• 2010

제주도는 우리나라의 가장 남쪽에 위하고 있으며 따뜻한 기온과 연중 축제 및 식물원, 박물관 등의 테마파크가 많아 온국민이 가장 선호하는 관광지역이다. 식물을 이용한 지역축제 및 관광명소 만들기는 초기비용이 적게 들어가고 친환경적이며 날이 갈수록 부가가치가 커지는 장점을 가지고 있다. 본 연구에서는 현재 제주도에서 행하여지고 있는 축제의 특성을 간단히 알아보고 자원식물을 이용한 제주도에서 연중 가능한 축제 프로그램을 개발하고자 하였으며 결과는 다음과 같았다. 1. 제주도의 축제는 총 31개이었으며 그중에서 식물을 이용한 축제는 15개로 전체의 48%를 차지하였다. 2. 지역에 따른 구분을 보면 제주시의 전체 축제는 16개이었으며 그중에서 식물을 이용한 축제는 8개로 전체의 50%를 나타냈고 서귀포시는 15개중에서 7개로 절반이 안되는 47%이었다. 3. 자원식물을 이용한 제주도에서의 연중 축제 프로그램 개발에 있어서 50여개를 제안하며 봄의 3월은 동백꽃축제, 수선화축제, 칡캐기축제, 서향축제, 4월은 꽃잔디축제, 개나리축제, 고사리축제, 조팝나무축제, 모란 축제, 개복사꽃축제, 살구꽃축제, 유채꽃축제, 청보리축제, 새우란축제, 왕벚꽃축제, 진달래축제, 5월은 참꽃나무축제, 대나무축제, 해당화축제, 작약축제, 라일락축제 등이 가능하다. 4. 여름의 6월은 한라산철쭉축제, 꽃창포축제, 접시꽃축제, 우리밀축제, 7월은 풍란축제, 홍화축제, 도라지축제, 수련축제, 산수국축제, 배롱나무축제, 8월은 해바라기축제, 무궁화축제, 다알리아축제, 칸나축제, 다육식물축제, 하귤축제, 열대과수축제, 등이 가능하다. 5. 가을의 9월에는 꽃무릇축제, 미로찾기축제, 10월에는 감국축제, 구절초축제, 11월에는 닥나무한지축제, 국화축제, 제주한란축제, 포인세티아축제 등이 가능하다. 6. 겨울의 12월에는 제주자생 구상나무를 이용한 크리스마스트리 경연대회, 1월에는 납매꽃축제, 먼나무축제, 2월에는 매화축제, 풍년화축제 등이 가능하다.

• Journal of the Korean Institute of Landscape Architecture
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• v.13 no.1
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• pp.95-107
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• 1985

This study is purposed to investigate the role of grasses as the Green Foundation effect on the visual preference to flowering tree and shrub being the principal elements of natural landscape early in the spring. As the flowering shrub materials, Rhododendron mucronulatum and Forsythia Kreana were adopted. Total 48 slides were photographed at the 8 different lawn areas with the 6 planting combinations of flowering shrub materials, and 10 landscape variables - dimensional and color - were measured and preference scores were taken by slide evaluations. The results were : 1) The visual preference to the landscape of flowering shrub in the lawn area was changed with the different lawn situations. 2) With important 4 variables, multilinear regression model was established, hence Y =40.4 + 9.6($X_1$) -7.8($X_2$) -26.8($X_3$) + 15.2($X_4$) where, Y : estimated preference score $X_1$: perimeter of flower zone $X_2$: value of green covered zone $X_3$: hue of green covered zone $X_4$: chroma of green covered zone 3) Most effective variable was 'hue of green covered zone', hence the more green the lawn area ism the more preferred landscape or the more effective green foundation is.

• Journal of Korean Society for Atmospheric Environment
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• v.4 no.1
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• pp.33-44
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• 1988

For the purpose of examining the effects of simulated acid rain on the chemical properties of the experimental soil in Pinus densiflora seedlings and Forsythia koreana rooted cuttings, the experimental design of randomized block arrangement with three replications was implemented in the experimental field of Yesan National Agricultural Junior College. One-year-old Pinus densiflora seedlings and Forsythia koreana cuttings were planted in the pots filled the mixed soils (nursery soil: forest soil of siliceous sandy loam = 1 : V/V)in the early spring of 1985. The regime of artificil acid rain, in terms of spray frequency per monthly and spray amount at single treatment per plot, was simulated on the basis of climatological data averaged from 30 years records. Simulated acid rain (pH 2.0, pH4.0, and pH 5.5 as control) containing sulfuric and nitric acid in the ratio of 3:2 (chemical equivalant basis) diluted ground water, were treated on the experimental plants under condition of cutting off the natural precipitation with vinyl tunnel, during the growing season (May 1 to August 31) in 1985. THe results obtained in this study were as follows; 1. Soil acidity was dropped, and exchangeable aluminum contents in the soil was dramatically increased in both species, with decreasing pH levels of acid rain. 2. Exchangeable potassium, clacium, magnesium contents, and base saturation degree of the soil were highly drcreased in two species as the pH levels of acid rain decreased. 3. In two species, sulfate concentrations in the soil were decreased of pH 4.0 treatment, and remarkably increased at pH 2.0 treatment of acid rain in comparison with control. 4. Total nitrogen and available phosphate contents of the soil were not affected by acid rain treatment in the both species, and Fe contents at pH 2.0 treatment were highest among three acid rain treatments.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.33-33
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• 2021

자웅산(264.3m)은 DMZ 접경지역인 경기도 파주시 법원읍에 위치하며, 백두대간 줄기 중 한북정맥을 따라 동쪽으로는 노고산(401m), 서쪽으로는 명학산(220m) 사이에 위치하고 있다. 2020년 4월부터 10월까지 계절별로 1회씩 총 3회 식물상 조사를 진행하였고 화상자료와 증거표본을 확보하였다. 본 조사로부터 자웅산 일대에 자생하는 관속식물은 82과 208속 267종 9아종 22변종 4품종으로 총 303분류군이 확인되었다. 양치식물은 10과 18분류군, 나자식물은 2과 6분류군, 쌍자엽식물이 62과 226분류군, 단자엽식물이 8과 53분류군으로 확인되었다. 개나리(Forsythia koreana (Rehder) Nakai), 백운산원추리(Hemerocallis hakuunensis Nakai), 키버들(Salix koriyanagi Kimura ex Goerz) 등 3과 3분류군의 특산식물이 확인되었다. 침입외래식물로는 가시도꼬마리(Xanthium italicum Moretti), 단풍잎돼지풀(Ambrosia trifida L.), 미국개기장(Panicum dichotomiflorum Michx.), 서양민들레(Taraxacum officinale F.H.Wigg.), 애기망초(Conyza parva Cronquist) 등 7과 23분류군으로 조사되어 조사식물 303분류군의 7.6%를 차지하였다. 환경부 지정 식물구계학적 특정종은 V등급이 미나리냉이(Cardamine leucantha (Tausch) O.E.Schulz), IV등급이 당단풍나무(Acer pseudosieboldianum (Pax) Kom.), 산복사나무(Prunus davidiana (Carrière) Franch.) 등 2분류군, III등급이 금낭화(Dicentra spectabilis (L.) Lem.), 꽃쥐손이(Geranium platyanthum Duthie), 분비나무(Abies nephrolepis (Trautv. ex Maxim.) Maxim.) 등 7분류군이 확인되어 III등급 이상의 특정종은 10분류군으로 파악된다.

• Journal of Korean Society of Forest Science
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• v.55 no.1
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• pp.37-46
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• 1982

These studies were carried out to know the measure of damage having an effect on the ornamental trees owing to sulfurous acid gas($So_2$) which injures much forest trees among air pollutions in south area of Korean and to know the difference of smoke resistance on the five representative species among them and therefore to establish the preventive measure of air pollutions in industrial complex of south area of Korea by the ornamental trees. The reaction formula utilized was $Na_2So_2+H_2So_4$ $Na_2So_4+H_O+So_4$. The results obtained were as follows. 1) The ornamental trees surveyed in the industrial complexes of Ul-San, Chang-Won and Po-hang belonged to 51 species, 161,699 trees in all. The most five species among 51 sorts of woody plants were Populus tomentiglandulosa, Buxus microphylla, Forsythia korenan, Juniperus chinensis and Pinus densiflora. 2) The relation between the concentration of sulfurous acid gas($So_2$) and the damage rate upon investigation in each species: In case of low concentration the destructive quantities of cells of woody plants, and the more increased the concentrations, the more increased the amount of damage. 3) The difference of smoke resistance among five species showed to be the strongest in Buxus micorphylla, the second rand Pupulus tomentiglandulosa, the third rand Forsythia koreana, the fourth rank Juniperus chinensis and the weakest in Pinus densiflora. 4) The ornamental trees of industrial complex expecially in south area of Korea in future should be planted gradually broad-leaved trees which have strong sprout, smoke resistance and thick leaved rather than comifers. 5) I think that the area of city forest in industrial complex requires about $50m^2$ per head of population and about 100m width of green belt between industrial area and residential district.

• Korean Journal of Remote Sensing
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• v.38 no.5_1
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• pp.627-646
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• 2022

Recently, the seriousness of climate change-related problems caused by global warming is growing, and the average temperature is also rising. As a result, it is affecting the environment in which various temperature-sensitive creatures and creatures live, and changes in the ecosystem are also being detected. Seasons are one of the important factors influencing the types, distribution, and growth characteristics of creatures living in the area. Among the most popular and easily recognized plant seasonal phenomena among the indicators of the climate change impact evaluation, the blooming day of flower and the peak day of autumn leaves were modeled. The types of plants used in the modeling were forsythia and cherry trees, which can be seen as representative plants of spring, and maple and ginkgo, which can be seen as representative plants of autumn. Weather data used to perform modeling were temperature, precipitation, and solar radiation observed through the ASOS Observatory of the Korea Meteorological Administration. As satellite data, MODIS NDVI was used for modeling, and it has a correlation coefficient of about -0.2 for the flowering date and 0.3 for the autumn leaves peak date. As the model used, the model was established using multiple regression models, which are linear models, and Random Forest, which are nonlinear models. In addition, the predicted values estimated by each model were expressed as isopleth maps using spatial interpolation techniques to express the trend of plant seasonal changes from 2003 to 2020. It is believed that using NDVI with high spatio-temporal resolution in the future will increase the accuracy of plant phenology modeling.

• Journal of Korean Society of Forest Science
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• v.13 no.1
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• pp.25-39
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• 1971

Recently successful induction of haploid plant by means of anther culture method has become a big topic among geneticists and plant breeders. The haploid plant can be used as a precious material for such basic researches as mutation or genetics. Once the haploid is obtained, production of homozygous plant is not a difficult problem. The method of producing homozygous plant can, also, be applied to the practical breeding works. When applied to the hybridization of self-fertilizing breeding period would be greatly shortened and in cross-fertilizing vegetables production of uniform hybrid seed would be very easily obtained. Last few years many scientists attempted anther cultures using various plant species, but it was successful only in several species. Unlike the other tissue cultures which use somatic organs or tissues as explants, anther culture seems to be very difficult because the plants or calli have to be induced from the haploid microspores or pollen grains. In the present experiment anther culture of fruit trees and ornamental shrubs of four genera and seven species was attemped. Anthers of Various stages ranging from tetrad and late microspore were cultured on the modified Murashige and Skoog's medium supplemented with various concentrations of auxins and kinetin as growth regulators. Handling of materials, sterilization, and other operations of culture were done by routine methods. The results were summarized as follows: 1. Calli were induced in the anthers of Forsythia Koreana Nak., Rhododendron mucronuratum Turcz., R. yedoense Max. var. Poukhanense Nak., and Prunus armeniaca L. var. ansu Max. No signs of callus were observed in Prunus persica Sieb. et Zucc. var. vurgaris Max., Pyrus ussuriensis var. macrostipes (Nak.), and Prunus salcina Lindley. 2. Calli were easily formed in any of the media with differing concentrations of auxins and kinetin. 3. In F. Koreana calli developed from anther surface and connective. Callus emerging out of anther locule was not observed. 4. Somatic calli arose from filament, connective, and inside of anther wall in R. mucronulatum. Many of the microspores accumulated starch grains. 5. The anther lobes located opposite the filament of R. yedoense turned easily to calli. This phenomenon was not observed in R. mucronulatum. Microspore embedded for a period in the medium became starch pollen. No callus was observed arising from microspore. 6. In P. armeniaca calli were not induced from somatic anther tissues. Instead, callus emerged out of anther locule rupturing the anther slit. Starch was not formed in the microspore. 7. In P. persica, Pyrus ussuriensis, and P. salcina, calli were not observed in the anthers examined more than 60 days after culture. Microspores of these species, however, were free of starch grains even after long period of subculture. 8. It was learned that somatic calli of the species examined arose usually from endothelium of anther wall, septum of two neighboring anther locules, parenchyma tissues of connectives, or anther lobes. 9. In the anther locule of P. armeniaca cultured long in medium, swollen microspores, polynucleate microspores, multicellular pollen grains, or callus mass were frequently observed, this indicating that the callus of this species was microspore-origin. 10. It was clarified that in P. armeniaca production of haploid plant by anther culture might be possible.