• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 2004.05a
• /
• pp.99-100
• /
• 2004

PCBs를 분석한 결과 붕어는 4개 지점에서 모두 검출되었으며 5.6염화 PCBs가 대부분을 차지하였고, 황소개구리는 2개 지점에서 5~7염화 PCBs가 일부 검출되었으며 나머지 시료에서는 검출되지 않았다.

• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 2004.05a
• /
• pp.101-102
• /
• 2004

유기염소계 농약류 14종을 분석한 결과 황소개구리의 경우 모든 시료에서 검출되지 않았다. 붕어의 경우 11종의 분석대상물질이 검출되지 않았고, heptachlor epoxide B, trans-nonachlor, p,p´-DDE가 각각 3개 지점, 7개 지점, 5개 지점에서 검출되었다.

• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 2003.11b
• /
• pp.377-378
• /
• 2003

Co-planar PCBs를 분석한 결과 붕어와 양서류 모두 전 지점에서 검출되었으며, 최대값은 황소개구리에서 1.622 pg-TEQ/g으로서 붕어의 최대값 0.974 pg-TEQ/g 보다 높게 나타났다.

• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 2003.11b
• /
• pp.379-380
• /
• 2003

PCBs를 분석한 결과 붕어는 전 지점에서 검출되었으며 3~7염소가 주로 검출되었고, 황소개구리는 4개 지점에서 2염소, 5~7염소가 일부 검출되었고 대부분의 시료에서 검출되지 않았다.

• Proceedings of the Korean Institute of Landscape Architecture Conference
• /
• 2014.03a
• /
• pp.89-90
• /
• 2014

• Development and Reproduction
• /
• v.10 no.2
• /
• pp.127-133
• /
• 2006

[ $17\;{\alpha}-hydroxylase/17,20-lyase(P450_{C17})$ ] is the key enzyme mediating the conversion of progesterone to $17\;{\alpha}-hydroxyprogesterone$, ultimately to androstenedione during steroidogenesis. R. dybowskii's ovarian $P450_{C17}$ cDNA was cloned to understand the regulatory mechanism of ovarian steroidogenic pathway at the molecular level in amphibian. A 2.5kb cDNA clone encoding a single open-reading frame with a 519 deduced amino acid was isolated with the screening of ovarian cDNA library. This sequence contained the three highly conserved domains as seen in $P450_{C17}$ of other species. The comparison of amino acid sequence of Rana $P450_{C17}$ with other animal's $P450_{C17}$ showed relatively high identity with 76% in Xenopus, 63% in chicken, 60% in rainbow trout, and 45% in human. Phylogenic analysis also indicated that Rana $P450_{C17}$ gene was evolutionary well conserved among vertebrate. Northern analysis indicated that the two different sizes of $P450_{C17}$ transcripts with approximately 2.5 and 3.6kb were detected in ovary tissue, but not in other tissues. The expression vector of Rana $P450_{C17}$ clearly showed the $17\;{\alpha}-hydroxylase$ activity converting the exogenous progesterone into $17\;{\alpha}-hydroxyprogesterone$ in the nonsteroidogenic COS-1 cells. Therefore, Rana $P450_{C17}$ cDNA is very useful to investigate the molecular mechanism of the ovarian steroidogenesis in amphibian.

• Korean journal of applied entomology
• /
• v.46 no.1 s.145
• /
• pp.13-18
• /
• 2007

Morphological characters of adult and immature stages on Elophila turbata (Butler, 1881) belonging to subfamily Nymphulinae, family Crambidae are redescribed base on Korean materials. Also we observed biological characters of this species including host plants, Spirodela polyrhiza(L.) Schleiden, Salvinia natans ($Linn\acute{e}$) Allioni and Lemna perpusilla Torre. And, photographs of adult, genitalia and immature stages are provided.

• Parasites, Hosts and Diseases
• /
• v.21 no.1
• /
• pp.83-86
• /
• 1983

A survey on the infection status of Sparganum and Gnathossoma in frogs was carried out in 11 areas in Gyeongsangnam-do and Cheonlanam-do province, a southern part of Korea. 1. Among 626 frogs examined, 4% were infected with Sparganum, but none with Gnathostoma. The infection rates of Sparganum were variable according to the areas. Those from Kimhae, Sunchon, Jinhae, Sachun, Kosung, Keoje, and Hadong were 16%, 9.1%, 9.1%, 4.1%, 2%, 2%, and 1.5% respectively, but frogs from Hapchun, Kwangju, Namwon and Sanchung were not found infected. 2. Rana nigromaculata was the only species infected with Sparganum. Rana rugesa and Bombing orientalis were not infected. 3. Sparganum was mainly found in the legs and the trunk of frog. About 92.5% of worms were detected in the femoral intermuscular connective tissue. 4. Number per infected frog was in range of one to five, and 2.8% of frogs were inacted by only one worm. 5. The heavier the frog weight, the higher the infection rate of Sparganum. In frogs of 51~60grams, 28.6% were infected.

• Journal of the Korean Chemical Society
• /
• v.8 no.4
• /
• pp.164-168
• /
• 1964

We isolated histone-type basic proteins from lemna paucicostata for the first time. Basic proteins were extracted directly with dilute mineral acids from homogenized lemna paucicostata. Amino acid compositions of basic protein portions adsorbed on Amberlite CG-50(at pH 6. 0) were resembled to those of calf thymus histones. Especially, lysine content was the greatest of the other amino acids. By chromatographic studies, adsorbed portions of basic protein components on carboxymethyl cellulose column(at pH 4. 2) were shown to be homogeneous to calf thymus histones, however, the area under the individual curve was different, and furthermore, the containing of a non-adsorbed portion in the large extent was markedly different from calf thymus histones. And amino acid compositions of adsorbed portions represented the histone-type basic propertes, but non-adsorbed portions were considered as a different protein compared with the typical histone. When calf thymus histone and protein components separated from lemna paucicostata were heated($60^{\circ}C$) with a solution of $HgSO_4-H_2SO_4$, precipitates were not obtained.

• The Korean Journal of Zoology
• /
• v.37 no.2
• /
• pp.161-173
• /
• 1994

면역효소법을 이용하여 3종의 한국산 개구리 참개구리(Rono nigromaculuto), 옴개구리(R. rugosa), 북방산개구리(R. 겨대bowskii)의 뇌에서 GnRH 뉴우런의 분포 부위와 GnRH의 종류 등을 연구하였다. 1차 항체로는 anti-rat GnRH, anti-salmon GnRH anti-chicken 11 GnRH 항체를 사용하였다. 3종의 개구리에서 mGnRH cGnRH 11와 sGnRH가 이둔 동정되었으나 3가지 항체에 대한 각 종의 면역 반응성은 종에 따라 달리 나타났다 mGnRH는 옴개구리와 참개구리에서, sGnRH는 북방산개구리에서 강한 면역 반응을 나타냈으며 cGnRH 11는 3종의 개구리에서 중간 정도의 면역 반응을 나타냈다. 각각의 GnRH의 상대적인 양에는 차이가 있으나 일부 경우를 제외하고는 뇌의 동일한 지역에 분포하였다. 참개구리에서는 GnRH가 중격 내측핵(NMS), Broca band 핵(NDB)에 집단으로 분포하였다. 북방산개구리에서는 GnRH가 중격 내측핵, Broca bnad 핵에서 등쪽에서 배쪽으로 길게 선상으로 가장 협소하게 분포하였으며, 번식기와 직전(1월-3월)에만 면역 반응을 나타냈다. 옴개구리의 뇌에서 가장 광범위한 지역, 즉 종뇌의 중격 내측핵, Broca band 핵, 아래 교차 지역(SCA)과 간뇌에 GnRH 신경세포가 분포하였으며. 제3뇌실 맥락얼기에서 mGnRH 신경세포가 처음으로 동정되었다. 3종에서 공통적으로 중격 내측핵과 Broca band 핵에서 유래한 신경섬유는 복측 시상하부를 거쳐 정중융기에 이르렀다. 이러한 결과는 GnRH가 뇌하수체에서 생식소 자극 호르몬의 분비 조절에 밀접한 관계가 있음을 뜻한다.