• Korean Journal of Medicinal Crop Science
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• v.13 no.3
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• pp.109-113
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• 2005

To analyze the genetic relationship 8 accessions of Ostericum koreanum Kitakawa, random amplified polymorphic DNA(RAPD) analysis was performed using 60 Operon primers. The 25 primers out of 60 random primers were amplified DNA by PCR using genomic DNA of O. koreanum. Eighty-five (49.1%) among 173 bands derived from 25 primers showed poly morphism, On the basis of similarity coefficient analysis by UPGMA, 8 accessions of O. koreanum Kitakawa could be classified into three groups at the similarity coefficient value of 0.71. Group I contained three accessions (Nam Gangwhal), Group II contained one accession (Nam Gangwhal) and Group III contained four accessions (Buk Gangwhal), The range of total genetic similarity coefficient value of 8 accessions of O. koreanum Kitakawa was $0.63{\sim}0.96$. Buk Gangwhal was flowered 18 to 26 days earlier than Nam Gangwhal, and Nam Gangwhal leaf stalk was thin and long as bolting rate high compared with Buk Gangwhal.

• Journal of agriculture & life science
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• v.44 no.2
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• pp.1-6
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• 2010

This study was carried out to investigate the effects of the various soil textures and NPK fertilizer application rates on growth and yield of Ostericum koreanum by pot experiment. Mean temperatures during cropping seasons of May, June, August and October were higher than those of normal year, but also the values were equal or lower for the other period. The growth and yield of Ostericum koreanum in the 1.5 fertilization rates became higher as the fertilizer rates increased by different soil textures. The content of soil organic matter and available phosphorus were high by application of high fertilization levels, while another contents did not show any significant differece. The yield of Ostericum koreanum was high in the loam soil by 278kg/10a. Those for clay soil was lower. Therefore, these results suggested that optimum soil texture for cultivation of this plant could be loam soil.

• Korean Journal of Plant Tissue Culture
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• v.22 no.5
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• pp.299-306
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• 1995

The objective of this study is to establish an efficient celll culture system for somatic embryogenesis in Ostericum koreanum and Angelica purpuraefolia. The highest frequency of embryogenic callus on immature seeds of O. konanum and A, purpuraefolia. was obtained when seeds were cultured on MS medium containing 0.1 mg/L 2,4-D and 0.1 mg/L BA. However somatic embryos were formed directly from the edge of cotyledon and hypocotyl of plant which regenerated on medium supplemented with 0.1-3.0 mg/L NAA. Immature seed explane cultured at 25$\pm$2$^{\circ}C$ after 10 days treatment at 5$^{\circ}C$ produced embryogenic callus and somatic embryos, and these differentiated into whole plane. Addition of glutamine and coconut milk to media did not enhance the frequency of somatic embryogenesis in immature seed cultures of A. purpuraefolia. However in immature seed culture of O. koreanum, the frequency of somatic embryogenesis were increased on media supplemented with glutamine and 10% coconut milk. Especially addition of glutamine to the medium substituted effect of NH$_4$N0$_3$ in constast to coconut milk. The highest frequency of conversion somatic embryos into plantlet was 89.1% on MS basal medium Embryogenic calli were grown vigorously when maintained on medium with 0.01 mg/L 2,4-D and 0.01 mg/L BA.

• Korean Journal of Medicinal Crop Science
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• v.15 no.1
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• pp.12-16
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• 2007

This study was carried out to investigate the cultivated soil properties, the growth and yield of Ostericum koreanum Kit. in the Bonghwa area. The results were as follows: In Bonghwa area, soil texture of upland soil were mostly sandy loam and loam. Sloped areas of $7\sim15%$ were plentiful than the other slopes, that of next was $15\sim30%$ slope. Sandy loam and loam soil were mostly 'well' in the drainage class, but loamy coarse sand and loamy sand soil were 'excessively well' drainage class. In the 'moderately well' drainage class, yield of Ostericum koreanum was 289kg/10a, and that of 'poorly' drainage class was low. The yield of loam soil texture was 284kg/10a. Soil organic matter, Potassium and EC were lower in 'poorly' drainage, but the other constituents were not definite tendency by different drainage classes.

• The Hankook-Saengyark Bo
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• no.89
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• pp.2-2
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• 1984

도깨비시장 외제약 거의 가짜

• Korean Journal of Plant Resources
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• v.25 no.5
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• pp.586-590
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• 2012

This study was conducted to investigate the seedling cultivation methods of Ostericum praeteritum Kitag. The results are as follows ; Ostericum praeteritum transplanted from wild seedlings and seedling cultured in the open field grew quite well, but their bolting rate was higher than other seedlings resulting in low root production. Seedling cultured in tray pot of green house produced good quality root as 426 kg/10a indicating the most adequate seedling method for Ostericum praeteritum production. Main compounds of Ostericum praeteritum roots were found as oxypeucedanin and bisabolangelone, and contents of wild seedlings were lower than theirs of the other seedlings methods.

• Korean Journal of Pharmacognosy
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• v.5 no.1
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• pp.7-8
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• 1974

• The Hankook-Saengyark Bo
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• no.248
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• pp.4-4
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• 2001

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.172-172
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• 1993

Acetaminophen(이하 AAP)은 간의 mixed function oxidase system에 의해 toxic metabolite로 대사되어 간괴사를 일으킨다. 최근에는 지금까지 임상적으로 사용되어온 한약이나 생약의 cytochrome P450의 활성과 간의 glutathion 합성에 미치는 영향에 대한 연구가 활발하게 진행되고 있다. 본 연구에서는 AAP의 간독성을 억제하는 물질을 규명하고자 민간이나 한방에서 보간 또는 해독제로 많이 사용되고 있는 생약을 screening하였다. 그 결과 간의 약물대사 효소계에 엉힝을 미치는 것으로 보고된 강활(Angelica koreana)과 민간이나 한방에서 간질환치료의 목적이나 해독제로 쓰여온 시호(Buplerum falcatum), 토복령(Smilax china) 및 금은화(Lonicera japonica)가 AAP로 유발된 간독성에 미치는 효과를 살펴보았다. 방법: 예비실험을 통해 강활, 시호, 토복령 및 금은화의 MeOH ext.가 AAP로 유도된 간독성에 방어효과가 있음을 확인하고 이를 hexane, ether, ethyl acetate 및 butanol로 계통분획하여 랫트에 전처치한 후 고농도의 AAP를 투여하여 간 손상을 일으키고 혈청중의 효소활성과 지질ㆍcholesterolㆍbilirubin함량 등을 측정하였다.

• 한국약용작물학회:학술대회논문집
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• 2007.11a
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• pp.284.1-284.1
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• 2007