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Investigation of in vivo Synergism of Mawhangyounpye-tang, a Poly-herbal Formula and Ciprof1oxacin against Streptococcus Pneumoniae Respiratory Infection (마황윤폐탕과 ciprofloxacin의 병용투여가 Streptococcus pneumoniae 호흡기감염에 대한 항균력에 미치는 영향)

  • An Tae-Ho;Song Kwang-Kyu;Jeon Kwi-Ok;Seo Young-Ho;Cho Dong-Hee;Park Mee-Yeon;Choi Hae-Yun;Kim Jong-Dae
    • The Journal of Korean Medicine
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    • v.26 no.2 s.62
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    • pp.13-24
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    • 2005
  • Objectives & methods; In order to evaluate the in vivo synergic effect of Mawhangyounpye-tang, a traditional poly-herbal formula used in the treatment of respiratory diseases in Korea, with the quinolone antibiotic ciprofloxacin (CPFX), the viable bacterial number and histopathological changes were monitored after experimental respiratory infection with Streptococcus Pneumoniae ATCC 6303. Results: 1. In CPFX groups, the viable bacterial numbers were significantly decreased compared to that of the control group, and were even more dramatically decreased in concomitant group treated with Mawhangyounpye-tang. 2. In the control group, severe infiltration of inflammatory cells, hemorrhage and hypertrophy of alveolar linings were demonstrated at microscopic levels. However, these abnormal histopathological changes were significantly decreased compared. to that of the control group in CPFX groups, and were even more dramatically decreased in concomitant groups treated with Mawhangyounpye-tang. 3. In CPFX groups, the LSA (Iuminal surface of alveoli $\%$) were significantly increased compared to that of the control group, and more dramatically in concomitant groups treated with Mawhangyounpye-tang. Conclusions: According to these results, it is considered that the in vivo antibacterial activity of CPFX against Streptococcus Pneumoniae ATCC 6303 infection of respiratory tract was dramatically increased by concomitant use of Mawhangyounpye-tang.

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Overwinter and Survival of Colletotrichum gloeosporioides and Glomerella cingulata in Soil and Plant Debris of Strawberry (딸기 병걸린 식물잔재물과 토양에서 탄저병균 Colletotrichum gloeosporioides와 Glomerella cingulata의 월동과 생존)

  • Nam, Myeong-Hyeon;Song, Jung-Young;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.10 no.3
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    • pp.198-202
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    • 2004
  • The overwinter and survival of Colletotrichum gloeosporioides and Glomerella cingulata in strawberry tissues under field conditions was investigated in 2001 and 2002. The rates of overwinter survival in plant petiole, runner and crown were 68.7,14.3, and 26.7%, respectively. But, under field condition, viable conidia of overwinter were not detected at the surface, 3, 5, and 10 cm depths of soil. To investigate the survival ability in soil and plant debris, C. gloeosporioides and G. cingulata isolates from strawberry were examined in laboratory and field. The viability of conidia was rapidly declined in all the treatments, with a 40% reduction in population within 10 days. In soil, the survival ability of G. cingulata conidia was similiar to that of C. gloeosporioides. The survival rate of conidia was highest under cool and dry soil conditions, and was decreased by increasing both soil temperature and moisture content. Similar results were also obtained under the condition that infected petioles were buried in soil. Results suggested that conidia as well as plant debris might be a main primary inoculum source of strawberry anthracnose.

Susceptibility of Vibrio vulnificus to Human Serum Bactericidal Activity and Effect of Vibrio Infections on Hematocrit Value in Mice (Vibrio vulnificus의 인혈청살균력에 대한 감수성과 Vibrio 감염이 마우스의 Hematocrit치에 미치는 영향)

  • Ha, Tai-You;Im, Suhn-Young;Chun, Sang-Nam;Kim, Chul-Kee
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.3
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    • pp.355-361
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    • 1986
  • Vibrio vulnificus, a halophilic Vibrio has gained worldwide attention as a cause of severe and frequently fatal wound infections and life-threatening septicemia. For this reason V. vulnificus is thought to produce extreme hemoconcentration and rapid death after infection, and because V. vulnificus is thought to be less susceptible to bactericidal activity of normal human serum, the present study was undertaken to assess the susceptibility of V. vulnificus to human serum bactericidal activity with that of V. parahemolyticus and V. cholerae and to assess the effect of Vibrio species, Salmonella typhimurium and E. coli on hematocrit values in experimentally infected mice. Serum bactericidal activity against both V. vulnificus and V. cholerae was higher than against V. parahemoltyicus. Survival of the test strains in heat-inactivated human serum was greater than that in heat-uninactiveted serum. Both V. parahemolyticus and V. cholerae produced slight hemoconcentration within 2 to 6 hr after intraperitoneal injection of $10^7$ viable bacteria into mice. In contrast, V. vulnificus, S. typhimurium and E. coli produced hemodilution rather than hemoconcentration after 4 or 6 hr after infection. With these results the author can conclude that V. vulnificus is more susceptible to serum bactericidal activity than other Vibrio species, and V. vulnificus did not produce hemoconcentration.

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Effects of Empowerment and Regarding Standard Precautions for Healthcare associated Infection Control in Healthcare Workers on Performances (의료종사자의 임파워먼트와 의료관련감염 관리 표준주의지침 인지도가 수행도에 미치는 요인)

  • Jung, Mi-Ra
    • Journal of Digital Convergence
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    • v.16 no.2
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    • pp.243-250
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    • 2018
  • The purpose of this study was to examine relationships among empowerment, awareness and performance on standard precautions and identify the factors that affect performance of standard precaution in the health care workers. The data were collected from 200 health care workers working in the general hospital located Y city. The data were analyzed by descriptive statistics, t-test, ANOVA, Scheffe, Pearson's correlation coefficient, and stepwise multiple regression. The result of the stepwise multiple regression indicates the awareness predict 49.0% (F=191.98, P<.001) in the performance of standard precaution. Therefore, it is necessary to develop awareness of the standard precaution strategy program for improving the performance of standard precautions in health care workers.

A small epidemiological survey for vivax malaria in Kimpo-gun, Kyonggi-do, Korea undertaken after detecting two consecutive cases (경기도 김포군 통진중학교 축구부원들에 대한 삼일열 말라리아의 역학조사)

  • 이종민;김미영
    • Parasites, Hosts and Diseases
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    • v.35 no.4
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    • pp.291-294
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    • 1997
  • On July and August 1997 two 15-year-old mates of a football team of Tongjin Middle School in northern Kyon99i-do, Korea were consecutively diagnosed as vivax malaria by peripheral blood smear. They had no histories of travelling abroad or drug abuse. Thry witnessed that othev mates in the tram were ill of fever in the same period. A small survey was therefore undertaken to determine whether vivax malaria was outbroken locally. A total of 57 students of the team living together in a dormitory was examined for history of fever. presence of splenomegaly, blood smear and anti-p. uiuax antibody test by immunofluorescent antibody test (IFAT) . Except for the above two patients, only one case rrvraled a marginal tiler of IFAT. No other positive findings of vivax malaria were found. In the results of this local survey. no move cases of vivax malaria were revealed except the two sporadic cases.

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A Clinical Analysis of 20 cases of Diaphragmic Rupture (외상에 의한 횡격막 파열의 임상적 고찰)

  • 이계선;정진악;금동윤;안정태;이재원;신제균
    • Journal of Chest Surgery
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    • v.32 no.4
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    • pp.394-398
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    • 1999
  • Background: According to the changes in the Environmental factors, traumatic diaphragmic rupture is seen in increasing frequency. Many reports described the early diagnostic methods and treatment modalities. In our institution, a study was retrospectively performed to obtain the early diagnostic and treatment methods of diaphragmic ruptures. Material and Method: From January 1994 to April 1998, 20 patients with traumatic rupture of the diaphragm were treated in our institution and We analyzed the patients in preoperative clinical presentations, diagnostic accuracies, associated injuries and postoperative complications. Result: Socially active male patients were affected most. 75% of patients had blunt trauma and 25% had penetrating injury. There were 16 cases of ruptured right diaphragm, 3 cases of left diaphragm and 1 case on both. Preoperative diagnosis were possible in 10 patients (50%) and 6 patients(30%) were diagnosed intraoperatively, but 4 patients (20%) were diagnosed in the late stages. Most common postoperative complication was wound infection, and two died of associated injuries. Conclusion: We conclude that if there is suspicion of diaphragmic rupture after a trauma, careful study and examination is essential and interdepartmental collaboration is very important.

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Broncho-Pleuro-Gastro-Colonic Fistula -A case report- (기관-흉강-위장-대장 누공 - 1예 보고 -)

  • Mun, Sung-Ho;Jang, In-Seok;Lee, Chung-Eun;Kim, Jong-Woo;Choi, Jun-Young;Rhie, Sang-Ho
    • Journal of Chest Surgery
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    • v.43 no.2
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    • pp.224-227
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    • 2010
  • A fistula between the respiratory and gastrointestinal systems is generally caused by infection and trauma. We experienced a 51-year old man with a broncho-pleuro-gastro-colonic fistula. He complained of chronic foul odor during respiration. He had suffered a traumatic diaphragmatic rupture 30 years ago. The infection of the diaphragm caused necrosis of the right lower lobe of the lung. It also caused a broncho-pleural fistula. The infection also created adhesion and a perforation of the gastric cardiac portion and the colonic splenic flexus portion of the gastro-intestinal track. We performed left lower lobectomy of the lung, reconstruction of the diaphragm and gastro-intestinal reanastomosis.

Surgical Treatment of Delayed Traumatic Anuerysm of the Innominate Artery - A case report- (외상 후 발생한 지연성 무명동맥류의 수술적 치료 -1예 보고 -)

  • Park Hoon;Keum Dong Yoon;Kim Hyung Tae;Koo Ja Hyun;Ko Sung Min;Choi Sae Young;Park Nam Hee
    • Journal of Chest Surgery
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    • v.39 no.2 s.259
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    • pp.162-165
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    • 2006
  • The innominate artery aneurysm is an uncommon entity. A 36-year-old man was transferred to our hospital because of incidental finding of right superior mediastinal mass. He had a history of blunt chest trauma due to automobile accident 16 years earlier. Computed tomography scanning demonstrated 5-cm sized sacular aneurysm with thrombus at the innominate artery. The prosthetic bifurcated bypass grafting from the ascending aorta to the right common carotid artery and right subclavian artery was performed under the moderate hypothermic cardioplumonary bypass. We report a successful surgical treatment for a rare case of the innominate artery aneurysm.

Microvascular Reconstruction of Extensive Foot Injuries (유리피판술을 이용한 족부재건)

  • Chung, Yoon-Kyu;Chung, Seum;Kim, Joo-Bong
    • Archives of Reconstructive Microsurgery
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    • v.3 no.1
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    • pp.64-71
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    • 1994
  • The successful treatment of the extensively traumatized foot warrants reconstruction utilizing tissue that will provide adequate coverage, is resistant to infection, thin enough to conform to the contours of the foot as well as durable to constant frictional movement and weight bearing. Currently, free flaps offer the best means in achieving these difficult and sometimes contradictory goals. We treated twenty-one patients suffering from extensive soft tissue loss of the foot due to trauma, electric burn or postburn sequelae with free flaps. A fasciocutaneous, musculocutaneous or muscle flap with skin grafting was used based on the location, volume of tissue required, and the functional anatomical requirement of the injured region. The follow-up duration averaged twenty-nine months. From our group of patients, we believe that the muscle free flap with skin grafting offers the most favorable outcome.

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The efficacy and influence on growth of olive flounder Paralichthys olivaceus vaccinated against Edwardsiella tarda and Streptococcus iniae (넙치, Paralichthys olivaceus에 투여된 Edwardsiella tarda와 Streptococcus iniae에 대한 혼합백신의 예방효과와 성장에 미치는 영향)

  • Kim, Myoung-Sug;Kim, Kyoung-Duck;Kim, Kang-Woong;Park, Myoung-Ae;Kim, Jin-Woo
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.327-334
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    • 2009
  • This study was performed to verify the efficacy and safety of vaccine mixed formalin killed Edwardsiella tarda and formalin killed Streptococcus iniae in olive flounder Paralichthys olivaceus. Sera were obtained from each group at 2, 4, 6 and 8 weeks after vaccination and agglutination titers to E. tarda and S. iniae were determined using the microtiter method. Three weeks after vaccination, the fish challenged by intraperitoneal injection of E. tarda or S. iniae and immunization resulted in a relative percentage survival (RPS) of above 81.0-92.8%. Neither the groups vaccinated nor control groups resulted in significantly reduced weight gain 140 days post-vaccination, but differences between the groups vaccinated and control groups were found at the early days post-vaccination. This results suggest that it is possible to prevent edwardsiellosis and streptococosis after a vaccination without affecting growth of olive flounder.