• Journal of Ginseng Research
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• v.23 no.2 s.54
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• pp.105-114
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• 1999

To investigate the effects of korean ginseng on the expression of transferrin receptor (TfR) in the liver cell membrane, we had carried out the experiments of $[^{3}H]thymidine$ uptake, $^{125}I-transferrin$ binding, and TfR mRNA expression in the liver after partial hepatectomy of normal and 3'-methyl-4-dimethylaminoa-zobenzene (3'-Me-DAB) treated rat with or without treatment of korean gingseng. $[^3H]thymidine$ uptake was not changed in the liver of 3'-Me-DAB or ginseng treated rat compared to that of control rat, but increased in that of partial hepatectomy of normal or 3'-Me-DAB treated rat. And this increased $[^{3}H]thymidine$ uptake was lowered slightly by the treatment of ginseng. Transferrin binding sites in the liver plasma membrane of ginseng treated rat with or without partial hepatectomy were similar, but increased in that of 3'-Me-DAB treated rat with or without partial hepatectomy compared to those of each control rat and these increased binding sites were reduced by ginseng treatment. Transferrin binding affinity (l/kd) was not changed by ginseng treatment, but tended to decrease in the liver of 3'-Me-DAB treated rat or in those after partial hepatectomy of all groups and reverse by ginseng treatment in 3'-Me-DAB treated rat. The expression of TfR mRNA was increased in the liver of 3'-Me-DAB treated rat with partial hepatectomy (peak at 24 hours), but lowered by ginseng treatment in this rat. From these results, it is suggested that korean ginseng has no effect on the increased expression of TfR with decreased affinity in the cell membrane of regenerated liver after partial hepatectomy of rat, but could inhibt that of 3'-Me-DAB treated rat through the regulation of DNA synthesis or TfR mRNA in partial.

• 건강소식
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• v.38 no.12
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• pp.20-21
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• 2014

겨울에는 평소보다 몸을 웅크리고 활동량이 줄어 체중이 늘기 십상이다. 더욱이 따듯한 실내에만 머물고 싶은 생각이 간절하다. 이런 때일수록 운동이 필요한 법. 따뜻한 실내에서 즐길 수 있는 운동으로 포켓볼보다 좋은 게 또 있을까. 천천히 걸으면서 이야기를 나누고 게임하는 동안 차 한잔을 곁들일 수 있는 흔치 않은 레저 스포츠로, 게임하는 재미에 운동 효과까지 제대로 얻을 수 있다.

• 건강소식
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• v.10 no.3 s.88
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• pp.12-15
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• 1986

이번 제4차 전국 장내 기생충 감염율 실태조사가 바야흐로 진행되는 시점에서 우리나라의 기생충학 수준 뿐 아니라 우리국민의 문화적인 긍지와 단결심을 함깨 자랑하게 되기를 바라는 마음 간절하다. 부디 온 국민이 합심하고 협조하여 이번 실태조사가 성공적으로 수행되기를 다시 한번 지면을 통해 부탁 하고져 한다.

• Proceedings of the KOSOMBE Conference
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• v.1994 no.12
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• pp.117-120
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• 1994

수술 전 간 기능이 저하되어 있는 간암환자의 경우 너무 많은 간 조직을 절제하면 남아있는 조직의 기능만으로는 생존하기 어려울 때가 있다 그러므로 수술전 간 기능의 평가를 정확히 할 수 있고 간절제 후에 잔여세포의 기능을 정확히 측정하는 것은 매우 중요한 의미를 갖게 된다. 현재의 일반적인 간 기능 검사나 Indocyanine 등을 이용하는 방법으로는 수술 후 잔여 간세포의 기능을 예측할 수 없다. 그리하여 본 연구에서는 수학적 deconvolution 방법을 이용하여 Hepatic extraction fraction(HEF)를 구하여 수술 후 잔여 간세포의 기능을 정량적으로 예측할 수 있는 software를 개발하고자 한다. 그리하여 간암 환자의 수술여부 및 절제부위의 크기를 결정할 수 있으며 또한 HEF로 신생아 황달의 원인인 간염과 간담도 폐쇄를 식별할 수 있다.

• 방재와보험
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• s.108
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• pp.36-41
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• 2005

예로부터 이어져온 불과 관련되 주술적 액막이 풍속에는 재물과 인명을 앗아가는 화재관련 재난을 피하기 위한 개인 또는 집단 형태의 간절한 기원과 믿음이 담겨있다. 소금이나 물과 같이 불과 상극인 상징물을 이용하거나 마을 단위의 제를 올려 화기를 막고자 했던 풍수적 자연관과 속신을 통해 화재에 대한 나름대로 방비책을 세우고자 했던 다양한 노력의 면면을 엿볼 수 있다.

• The Korean Journal of Pharmacology
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• v.29 no.1
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• pp.85-96
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• 1993

To investigate the alteration of transferrin receptor (TfR) in the proliferating or transformed liver cells, $^{125}I-transferrin$ binding experiment was carried out in the isolated parenchymal cells (PC) or nonparenchymal cells (NPC) from normal regenerated rat liver after partial hepatectomy and from the liver of 3-methyl-4-dimethyl-aminoazobenzene (3-Me-DAB) treated rat. With the administration of 3-Me-DAB for 8 weeks, the liver tissue showed marked morphologic changes of oval cell proliferation, regenerations of hepatocytes, and atypical proliferations of bile ducts, but these changes were little affected by partial hepatectomy. Transferrin binding values in PC or NPC homogenate from the regenerated liver of normal rat, were increased by 3rd day and diminished to control level at 7th day after partial hepatectomy. With the treatement of 3-Me-DAB for 8 weeks, transferrin binding sites in homogenates were higher than those of normal rat liver and increased by 7th day after partial hepatectomy. Transferrin binding sites (Bmax) in the cell membrane of NPC were higher than those of PC of normal rat liver, but there was no significant difference in Kd values between both groups (5.05, 6.3 nM). In the normal resenerated rat liver, transferrin binding sites in the PC or NPC plasma membrane, were increased by 3rd day and diminished to control level at 7th day after partial hepatectomy. With 3-Me-DAB tratment, transferrin binding sites in both liver NPC and PC plasma membrane were increased about 3 folds, compared to those in each plasma membrane of normal rat liver. And after partial hepatectomy of 3-Me-DAB trated rat, transferrin binding sites were increased by the 3rd day in the NPC plasma membrane but increased by the 7th day in the PC plasma membrane. In the transferrin binding sites of the PC or NPC plasma membrane of 3-Me-DAB treated liver, two kinds of Kd values $(3.1{\sim}4.7\;nM,\;25.4{\sim}54.1\;nM)$ were detected. The present results suggest that 1) TfRs are distributed in the liver PC as well as NPC; 2) Increased TfRs in PC or NPC plasma membrane of normal regenerated liver after partial hepatectomy and 3-Me-DAB treated rat liver, may be due to increased intracellular synthesis; 3) Increased TfRs in normal regenerated liver after partial hepatectomy might be related to the expression of a single type of high affinity site $(Kd,\;3.1{\sim}7.5\;nM)$, but in 3-Me-DAB treated rat liver might be related to the expression of high and low affinity types of receptors $(Kd,\;25.4{\sim}54.1\;nm)$.

• 월간 기계설비
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• s.81
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• pp.76-85
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• 1997

현장에서 공사에 참여하다 보면 작업이 번거롭고 공수가 제일 많이 들어가는 공정이 아파트, 호텔 등에서의 화장실과 욕실 배관이다. 필자가 지난 80년부터 설비공사업계에 몸담아 오면서 아파트에 있어서 위생배관 시공밥업에 변화가 있었다면 UBR이 개발된 것 외에는 별다른 기술개발이 이루어지지 않고 있는 실정이다. 건설분야는 3D업종에 속해 기술을 배우려는 젊은이들이 줄어들고 있어 심각한 문제이다. 우리 설비공사업계가 건설시장 개방에 대비하여 살길을 찾기 위해서는 새로운 기술개발이 최우선 과제이다. 이러한 문제의 근본적인 해결에 기여를 했으면 하는 간절한 바램과 함께 국내 설비기술 수준 향상에 조금이나마 도움이 되었으면 하는 뜻에서 화장실 배관의 배선공법등을 적어 보았다.

• Nuclear Medicine and Molecular Imaging
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• v.43 no.5
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• pp.429-435
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• 2009

Purpose: The purpose of this study was to assess the prognostic value of preoperative FDG-PET in colorectal cancer (CRC) patients with hepatic metastasis (HM). Materials and Methods: 24 CRC patients (M:F=14:10; age, $63{\pm}10$ yrs) with HM who had undergone preoperative FDG PET were included. Cure-intent surgery was performed in all the patients and HMs were controlled using resection (n=13), radio-frequency ablation (RFA) (n=7), and resection plus RFA (n=4). Potential prognostic markers tested were maxSUV of primary tumor, maxSUV of HM, maxSUV ratio of HM over primary tumor (M/P ratio), histologic grade, CEA level, venous/lymphatic/nerve invasion, T stage, N stage, no. of HM, no. of lymph node metastasis, and treatment modality of HM. Results: 14 CRC patients developed a recurrence with a median follow-up duration of 244 days, whereas 10 patients did not develop recurrence with a median follow-up duration of 504 days. M/P ratios but other potential prognostic markers were significantly higher in the recurrent patients ($0.72{\pm}0.14$) than recurrence-free patients ($0.54{\pm}0.23$) (p=0.038). M/P ratio only was found to predict recurrence by Cox multivariate analysis (hazard ratio 37.7, 95% confidence interval 2.01-706.1, p=0.016). The 11 patients with lower M/P ratio of <0.61 had significantly better disease-free survival rate than the 13 patients with higher M/P ratio (${\geq}0.61$) (p=0.026). Conclusion: maxSUV ratio of HM over primary tumor (M/P ratio) may be useful for prognosis prediction of CRC patients with HM. Higher FDG uptake of HM than that of primary tumor may indicate a more advanced status in stage IV CRC.

• Journal of the KSME
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• v.19 no.3
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• pp.164-168
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• 1979

이상에서 논술한 바와 같이 금속절삭에 있어서는 절삭공구는 열의 와중의 격열한 상태하에서 작동이 이루어져야 하기 때문에 절삭유제를 분사함으로써 냉각작용으로 경도저하를 방지하고, 균체작용을 시킴으로써 열의 발생을 적게 하며 동력 소비를 감소함과 동시에 절삭면의 정밀도를 향상시킨다. 또한 절삭유제에 극압첨가제를 첨가함으로써 고체윤활을 생성케하여 용찰을 방지 하는 효과를 얻고 있다. 이 외에도 절살유제는 칩의 세척작용, 공작물과 기계의 방작용등의 효 과를 얻고 있다. 요는 절삭가공에서 절삭유제의 사용은 절삭공구의 수명을 연장하고 생산능률을 올리는 효과를 기대하는 것이라고 요약할 수 있다. 여기서 부차적으로 고려해야할 것은 절삭유 제의 토방법도 중요하다. 동일한 조건하에서는 토방법에 따라 효과가 다르다. 가장 효과적인 토 법은 공구의 앞여유면쪽으로 분사하는 것이 가장 좋으며, 칩 위에 분사하는 것이 가장 비효과 적이다. 이와 같은 절삭유제 작용과 효능을 알고 실제가공에 있어서 다소라도 도움이 되었으면 다행으로 생각하는 마음 간절하면서 끝맺는 바이다.

• Applied Microscopy
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• v.40 no.2
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• pp.73-80
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• 2010

Liver regeneration is a result of highly coordinated proliferation of hepatocytes and nonparenchymal liver cells. Partial hepatectomy (PH) is the most often used stimulus to study liver regeneration because, compared with other methods that use hepatic toxins, it is not associated with the tissue injury and inflammation, and the initiation of the regenerative stimulus is precisely defined. Granulocyte macrophage-colony stimulating factor (GM-CSF), which is a cytokine able to regulate the proliferation and differentiation of epithelial cells, was first identified as the most potent mitogen for bone marrow. Particularly, rrhGM-CSF, which is highly glycosylated and sustained longer than any other types of GM-CSF in the blood circulation, was specifically produced from rice cell culture. In this experiment, effects of rrhGM-CSF administration were evaluated in the regenerating liver after 78% PH of rats. Morphological changes induced by PH were characterized by destroyed hepatocyte plate around the central vein and enlarged nuclear cytoplasmic ratio and increased hepatocytes with two nuclei. And then, proliferation of liver cells (parenchymal and nonparenchymal) and rearrangement of plates and lobules seemed to be carried out during liver regeneration. These alterations in the experimental group preceded those of the control. Since proliferating cell nuclear antigen (PCNA) is known to be a nuclear protein maximally elevated in the S phase of proliferating cells, the protein was used as a marker of liver regeneration after PH in rats. PCNA levels by western blot analysis and immunohistology were compared between the two groups. PCNA protein expression of two groups at 12 hr and 24 hr after injury showed similar pattern. The protein expression showed the peak at 3 days in both groups, however, the protein level of the experimental group was higher than that of the control. On immunohistochemical observations, the reaction product of PCNA was localized at the nuclei of proliferating cells and the positive reaction in experimental group at 3 days was clearly stronger than that in control group. The results by Western blotting and immunohistology for PCNA showed similar pattern in terms of the protein levels. In conclusion, rrhGM-CSF administration during liver regeneration after 78% PH accelerated breakdown and restoration of the hepatic plate and expression of PCNA. These results suggest that rrhGM-CSF might play an important role during liver regeneration in rats.