• Korean Journal of Animal Reproduction
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• v.13 no.1
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• pp.49-55
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• 1989

The Results of colchicine and pretreatment time have shown that the cells of Coho Salmon(Oncorhynchus Kisutch) were effectively injected 6 hours prior to the harvest at a final concentration of 10$\mu\textrm{g}$/g body weight. The cell-density from 8,000 cells/㎣ (=8${\times}$106 ml) was found as ideal. The best effect of hypotonic solution in proportion to the cell is 20 : 1 and 50 minutes. The model number of diploid chromosome in this species was 2n=60. The karyotype analysis proved that the diploid complement consisted of 19 pairs of metacentric-, 4 pairs of submetacentric- and 7 paris of acrocentric-chromosomes. The diagrammatic representation proved that the diploid complement consisted of 7 pairs of metacentric-, 2 pairs of submetacentric- and 1 pair of acrocentric-chromosomes.

• Korean Journal of Veterinary Service
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• v.18 no.2
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• pp.177-181
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• 1995

The effects of histamine were investigated on the uterine smooth muscle motility in the pig. The results were summarized as fellows : 1. Histamine caused the contraction of the porcine uterine smooth muscle and the contractile responses increased between the concetration of histamine $10^{-8}$ and $10^{-5}$ M with a dose-dependent manner. 2. The contractile response Induced by histamine ($10^{-6}$ M) was completely blocked by pretrevatment with $H_1$-histaminergic receptor blocker, pyrilamine($10^{-6}$ M) 3. The contractile response induced by histamine($10^{-6}$ M) was increased by pretreatment with $H_2$-histaminergic receptor blocker, cimetidine($10^{-6}$ M) From these results, it was concluded that the effects of uterine smooth muscle by histamine were the contraction mediated by $H_1$-histaminergic receptor and the relaxation mediated by $H_2$-histaminergic receptor in pig.

• Korean Journal of Animal Reproduction
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• v.18 no.1
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• pp.31-37
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• 1994

This study carried out to investigate the effects of cryoprotectants in the medium on the survival rate of rapidly frozen porcine bisected demi-embryos. The porcine bisected demi-embryos following dehydration by cryoprotectants containing sucrose were directly plunged into liquid nitrogen and thawed in 3$0^{\circ}C$ water bath. Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows : 1. The survival rates of without-zona pellucida embryos and 2 blastomeres porcine embryos were 10.0 and 7.1%, respectively. The rate of unfrozen blastomeres (20.00%) was significantly higher than that of non-frozen oocytes. 2. The survival rates of with and without-zona pellucida of bisected porcine embryos by micromanipulator were 20.0 and 14.3%, respectively. 3. The developmental rates of with and without-zona pellucida of bisected poricine embryos by micromanipulator were 13.3 and 7.1%, respectively.

• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.161-162
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• 2010

• Korean Journal of Animal Reproduction
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• v.17 no.3
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• pp.257-262
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• 1993

Various tissue and follicular components were analyzed for the determination of lactate dehydrogenase(LDH) isozyme patterns by electrophoretic technique with chromogen reaction in the pig. Optimum conditions for the tissue homogenate and the storage were finally established. Small quantities of follicular components were analysed for typing of LDH isozymes by microelectrophoresis. Microelectrophoretic analysis showed that only LDH-1 was visible in the oocytes, all isozymes in cumulus masses, and LDH-1, 2 and 3 in follicular fluid. The results provide critical information on the LDH activity of various tissues and follicular components. Furthermore, t he developed methods should be useful the analysis of LDH in the small quantity of samples, especially in the oocyte, and easily applicable to the oocyte and early embryos of other domestic species.

• KOREAN POULTRY JOURNAL
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• v.37 no.10 s.432
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• pp.77-79
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• 2005

최근 우리나라도 전업축산농가의 양계사육 규모가 확대되면서 이에 수반되는 양계장 주변의 악취, 수질 오염 등의 환경문제가 심각하게 대두되고 있다. 축산경영의 건전한 발전을 위해서도 이 문제의 해결은 시급한 과제이며 특히 경영주의 환경의식과 의지가 문제해결의 시발점임을 인식해야 한다. 물론 양계장에서 환경친화적으로 계분을 처리한다는 것은 그리 용이한 일은 아니다. 특히 산란계 사육농가의 계분은 끈적끈적한 오물감과 냄새로 취급하기가 쉽지 않다. 그 원인은 수분 함유량이 높고 역분해성 유기물(분해 용이한 유기물)이 부패되면서 악취와 해충을 발생시키기 때문이다. 현재 계분처리방법은 여러 가지가 있으나 첫째, 지구환경에 친화적인 처리방법으로는 유기성 폐기물인 계분의 재활용에 있다고 생각한다. 현재와 같이 지구환경의 밸런스가 망가지는 상황은 사람들이 다량의 소비만 일삼고 환경 리싸이클에 관심이 덜한데 있다고 본다. 자원 순환형 사회에서 계분의 퇴비화로 가축분뇨를 유통 · 활용하는 일은 유기성 자원의 리사이클에 공헌하는 의미가 크다고 본다. 또한 우리나라의 축산업이 환경 보전형 농업을 실현하는데 보탬이 되는 양질의 퇴비를 대량 생산하여 경작농가에 공급, 유기질 농산물 생산에 많이 기여하길 바란다.

• KOREAN POULTRY JOURNAL
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• v.37 no.10 s.432
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• pp.85-89
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• 2005

우리나라 양계산업은 그동안 외형적으로 사육규모의 확대를 통해 소규모 내적으로도 사육시설의 현대화를 통하여 생산성의 향상과 양계산물의 고급화를 추구해왔다. 그러나 한편으로는 국민의 환경의식의 향상과 함께 환경문제가 크게 대두되면서 환경오염방지에 대한 요구도 더욱 커지고 있으며 가축사육의 규모화 및 단지화는 지역집중화를 초래하고 계분 이용의 불균형을 심화시키는 한 원인이 되고 있다. 또한 닭 사육밀도가 지역에 따라 이미 적정선을 웃돌아 계분의 관리 잘못으로 토양오염, 대기오염, 수질오염 등의 역기능을 유발하고 있다. 생계분 조차도 돈을 받고 판매하던 시절은 이제 옛이야이가 되었고 공을 들여 잘 제조한 양질의 퇴비 조차도 판매처가 없어서 창고에 쌓아놓고 판매처를 찾아야 하는 어려움을 겪고 있는 실정이다. 그러나 1962년부터 시작된 화학비료에 대한 보조금을 2005.7.1부터 폐지되었으며 동시에 화학비료의 보조금을 유기질비료에 확대지원하고 있어서 앞으로 유기질비료의 이용은 더욱 증가할 것으로 예상된다.

• Korean Journal of Animal Reproduction
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• v.16 no.4
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• pp.289-299
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• 1993

The polypeptide patterns of cellular and follicular components were analysed by SDS-PAGE and two dimensional(2-D)electrophoresis combined with isoelectric focusing (IEF) to establish protein profiles in each of the components in porcine follicles. Oocyte-cumulus complexes were cultured in M16+FCS+Gn at 39 in an atmosphere of 5% CO$_2$, in air for 35 h. At the end of the culture, the zona-free oocyte, ZP alone and cumulus cells were prepared and analysed either on 10% SDS-PAGE for the protein profile at the first dimensional gel or 2-D protein pattern. The amounts of each samples were determined for the visualization with Coomasie brilliant blue (CBB) or silver staining, thus giving useful information for the identification of specific proteins in the components or appropriate amount of samples for proper visualization. Oocyte showed 25 and 114 kd major protein band. Other minor components were additionally visualized with CBB on the same gel after silver staining procedure. Cumulus cells also showed specific proteins which is not present in the oocytes. The number of cumulus cell was proper to give major bands with CBB and additional minor bands with silver staining. To establish the degree of contamination from the remnant of the corona radiata to the ZP, zonae were differently prepared or analysed by SDS-PAGE.The preparation of the ZP in this study did not showed any contamination judged by the protein profile of the components. Also follicular fluid showed its specific protein profile without any significant differences among the different sizes of follicles. The established protein profile of each follicular component should be helpful for the identification and elimination of contaminated components, i. e., antigen preparation or immunological studies. The results also suggest that the preparation of each components in the study was appropriate and can be used for a further sensitive biochemical analysis in mammalian oocytes and early embryos.

• Korean Journal of Animal Reproduction
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• v.17 no.4
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• pp.339-346
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• 1994

An understanding of the structure and function of mammalian spermatozoa requires the iso-lation of these components. In this study, frozen-thawed bovine spermatozoa were treated by physical treatments (vortexing, 26 gauge needle, strained 26 gauge needles and freezing-thawing) or chemical treatments (trypsin, dithiothreitol, sodium dodecylsulfate and $\beta$-mercaptoethanoJ) to yield free heads and tails. The most effective treatment was repeated pumping of sperm suspension through a strained 26 gauge needle conneted to a syringe. Spermatozoa by this treatment were mainly broken at the junction of the head and the tail, resulting in 90-100% yields. Also, sperm head surface did not modify during strained 26 gauge needle treatment when either spermatozoa or sperm heads were incubated in 250${\mu}\textrm{g}$/ml of FITC-UEA 1 for 1 h at room temperature to detect the modification of sperm surface components. Other physical treatments were less efficient for the breakdown of spermatozoa. The effects of chemical treatments on bovine spermatozoa are not noticeable. Dissected sperm heads and tails should be fractional leading to nearly pure components by sucrose gradient centrifugation at 1,000 rpm for 15 min. The result suggest that the established method may be useful for the biochemical study of spermatozoal components, and the understanding of oocyte activation mechanism either by spermatozoal components during fertilization or microinjection of isolated components.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.409-413
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• 2014

BACKGROUND: Large amount of veterinary antibiotics have been used in the livestock industry to prevent diseases and promote growth. These antibiotics are excreted through feces and urine in unchanged form and reach to agricultural fields via application of the livestock manure based composts. The purpose of this study was to evaluate the occurrence of tetracyclines-resistant bacteria in the soil received livestock manure compost for a long term. METHODS AND RESULTS: Tetracyclines (tetracycline TC, chlortetracycline CTC, and oxytetracycline OTC) resistance bacteria in the soil of rice-onion field applied pig manure compost (PM), in the soil of grass-rye field received cow manure compost (CM), and in the soil of rice field applied inorganic fertilizer (NPK) were determined. The soil received livestock manure composts clearly showed higher number of TC, CTC, and OTC resistance bacteria compared with the soil treated with inorganic fertilizer. The antibiotic resistant bacteria recovered appeared at 80 mg/L of tetracyclines was identified 1 specie, 6 genera 7 species, and 6 genera 7species in the soils received CM, PM, and NPK, respectively. The dominant resistant bacteria with the CM and PM application were Ochrobactrum and Rhodococcus. CONCLUSION: The application of livestock manure compost in the agricultural field is likely to contribute the occurrence of antibiotic resistance bacteria in the agricultural environment.