• Korean Journal of Food Science and Technology
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• v.39 no.2
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• pp.122-127
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• 2007

In this study, an analytical method was optimized for detecting dioxin-like PCBs in fish and shellfish. Here, homogenized samples were extracted using an accelerated solvent extraction (ASE) system with 33 mL cell size. Multilayer column chromatography, which consisted of acidic, basic and neutral silica gels, was used for the clean up of the extracts. The instrumental analysis was executed by HRGC/HRMS to a resolution of 10,000 using 4 window multiple ion detection (MID) mode. For the results, the average recoveries ranged from 94.1 to 104.1% (${\pm}8.4$) and the limit of detection was approximately 0.1 pg/g at S/N ratio >3. Finally, the detected concentrations of dioxin-like PCBs for fish and shellfish were in the range of 0.030-1.836 pg TEQ/g.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.4
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• pp.604-608
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• 2002

Accelerated solvent extraction (ASE) was used for a rapid and simple extraction of propylenchlorohydrin (PCH) residue in hydroxylpropyl modified starch. The effects of temperature, pressure and extraction solvent on the extraction efficiency were investigated to find the optimal condition of ASE. The optimal conditions for PCH extraction in hydroxylpropyl modified starch were static time of 50 min, purge time of 300 sec, heating time of 5min, temperature of 12$0^{\circ}C$, pressure of 2500 psi, flush (%) with 100 volumes, and ethylacetate as an extraction solvent. The recovery (96.1%) of this method was higher than that (76.4%) of Code of Food Additive. Therefore, the ASE was a good method in both aspects of efficiency and effectiveness.

• Journal of Soil and Groundwater Environment
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• v.7 no.2
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• pp.3-11
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• 2002

The objective of this study was to survey the oil contamination around the Mountain Baegun at Uiwang city to obtain the preliminary data for bioremediation. For measuring the oil concentrations and physical properties from soil, we analyzed BTEX. TPH and pH, organic content, water content, pormeability coefficient, gravity, porosity and used the purge & trap method for analyzing BTEX. Using the Accelerated Solvent Extractor, we pretreated the samples and then analyzed TPH using GC-FID as soon as possible. From the analysis results, maximum concentration of TPH was 24.773mg/kg and BTEX was 101.7mg/kg. The results of TPH at the Mountain Baegun were higher than the enforcement standard of soil contamination(Korea) and the BTEX concentrations were also higher than the advisory standard of soil contamination(Korea). From these results, the Mountain Baegun may requires to remedy the oil-contaminated soil. In addition, we performed the field bioremediation test for five weeks at the Mountain Baegun using the microbial additives that were developed by our laboratory. From the results of the field test, we could find the about 95% of the oil was removed from the contaminated soil in five weeks. So we consider that it is the one of the useful solutions to remedy the oil-polluted site.

• Food Engineering Progress
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• v.15 no.4
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• pp.289-296
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• 2011

Twenty four fractions by solvent extraction and/or acid precipitation from fruit body and culture broth of Inonotus obliquus were prepared, and their inhibitory effect against acetylcholinesterase (AChE) was investigated. Among these fractions, acid (1 M HCl) precipitates from cell-free culture broth and fruit body exhibited the highest inhibitory effect on AChE in vitro. Acid precipitates inhibited AChE activity in a concentration-dependant manner and $IC_{50}$ values of both acid precipitates were 0.53 mg/mL. The inhibition pattern was general non-competitive inhibition. The energetic parameters were also determined by dual substrate/temperature design. Both acid precipitates increased the values of Ea, ${\Delta}H,/;{\Delta}G$ and ${\Delta}H^{\ast}$ decreasing the value of ${\Delta}S$ for AChE. The results implied that the acid precipitates from I. obliquus increased the thermodynamic barrier, leading to the breakdown of ES complex and the formation of products as inhibitory mechanism.