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Current Research Trends of Wheat Transformation and Biotechnology (밀 형질전환과 이를 활용한 최신 연구동향)

  • Sim, Jae-Ryeong;Kim, Sewon;Lee, Su-Bin;Kim, Beom-Gi;Lee, Saet Buyl;Lee, Jong-Yeol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.386-398
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    • 2020
  • Wheat is one of the world's top three crops and is an important staple crop, accounting for 20% of the nutrient calories consumed by the world's population. However, due to its complex heterogeneous hexaploid chromosomes and vast genome of approximately 16 Gb, compared to those of other crops, molecular biology and biotechnology studies on wheat are lacking. In recent years, wheat genome analysis has been performed using the latest next-generation sequencing technology so that useful genes can be easily obtained, and wheat biotechnology research is accelerating in various fields. In this review, wheat transformation, an indispensable technique for developing new functional biotech wheat by revealing the function of wheat genes, is described in detail. In addition, the latest research results for overcoming plant diseases, abiotic stresses, and wheat-related diseases that are difficult to solve by classical breeding through wheat transformation and biotechnology are described.

Change in Fluorescence Characteristics of Dissolved Organic Matter at Inflow Stream per Catchment of Different Land Use (토지이용도가 다른 소유역별 유입하천에서 용존유기물 형광특성 변화)

  • Kim, Sea-Won;Oh, Jong-Min;Lee, Bo-Mi;Choi, Kwang-Soon
    • Korean Journal of Ecology and Environment
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    • v.44 no.3
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    • pp.292-302
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    • 2011
  • The Lake Sihwa watershed includes industrial, urban, and rural areas simultaneously. In this study, we analyzed the characteristics of dissolved organic matter (DOM) in spatial-temporal runoff patterns at representative sites having different land use in the watershed of Lake Sihwa. The result of synchronous and 3D-EEMs(3-Dimensional Excitation Emission Matrix Spectroscopy) analysis in 4TG (industrial area), fluorescence distribution and variation clearly appeared in the Fulvic-like fluorescence (FLF) and Humic-like fluorescence (HLF) regions along with the Protein-like fluorescence (PLF) region. A characteristic that Peak A (HLF) region fluorescence intensity did not decrease and the HLF region of fluorescence intensity and spatial-temporal changes clearly appeared during rainfall in AS (urban area). The results of fluorescence analysis in MS did not show great changes in PLF and FLF while showing that fluorescence intensity changes over time in the Terrestrial-like fluorescence (THLF) region increased greatly. In conclusion, our results showed significant differences in the runoff characteristics of DOM particularly in industrial, urban and rural area, and these differences should be considered for the efficient controlling of DOM in the watershed.

A Cancer-specific Promoter for Gene Therapy of Lung Cancer, Protein Regulator of Cytokinesis 1 (PRC1) (폐암의 유전자 치료법을 위한 암특이적인 PRC1 프로모터)

  • Cho, Young-Hwa;Yun, Hye-Jin;Kwon, Hee-Chung;Kim, Hee-Jong;Cho, Sung-Ha;Kang, Bong-Su;Kim, Yeun-Ju;Seol, Won-Gi;Park, Kee-Rang
    • Journal of Life Science
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    • v.18 no.10
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    • pp.1395-1399
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    • 2008
  • We have recently reported the PRC1 promoter as a promoter candidate to control expression of transcriptionally targeted genes for breast cancer gene therapy. We tested whether the PRC1 promoter could be also applied for the lung cancer gene therapy. In the transient transfection assay with naked plasmids containing the luciferase fused to the PRC1 promoter, the promoter showed little activity in the normal lung cell line, MRC5. However, in the lung cancer A549 cells, PRC1 showed approximately 30-fold activation which was similar to the survivin promoter, the gene whose promoter has been already reportedas a candidate for the gene therapy of lung cancer. In viral systems, the PRC1 promoter showed approximately 75% and 66% of transcriptional activity compared to the CMV promoter in the adeno-associated virus (AAV) and the adenovirus (AV) systems, respectively. However, the PRC1 promoter in either AAV or AV showed approximately 20% activity compared to the CMV promoter in the normal lung cells. In addition, human lung tumor xenograft mice showed that the PRC1 promoter activity was as strong as the CMV activity in vivo. Taken together, these results suggested that PRC1 might be a potential promoter candidate for transcriptionally targeted lung cancer gene therapy.

Modeling of Welding Heat Input for Residual Stress Analysis (용접 잔류응력 해석을 위한 Heat Input Model 개발)

  • 심용래;이성근
    • Journal of Welding and Joining
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    • v.11 no.3
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    • pp.34-47
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    • 1993
  • Finite element models were developed for thermal and residual stress analysis for the specific welding problems. They were used to evaluate the effectiveness of the various welding heat input models, such as ramp heat input function and lumped pass models. Through the parametric studies, thermal-mechanical modeling sensitivity to the ramp function and lumping techniques was determined by comparing the predicted results with experimental data. The kinetics for residual stress formation during welding can be developed by iteration of various proposed mechanisms in the parametric study. A ramp heat input function was developed to gradually apply the heat flux with variable amplitude to the model. This model was used to avoid numerical convergence problems due to an instantaneous increase in temperature near the fusion zone. Additionally, it enables the model to include the effect of a moving arc in a two-dimensional plane. The ramp function takes into account the variation in the out of plane energy flow in a 2-D model as the arc approaches, travels across, and departs from each plane under investigation. A lumped pass model was developed to reduce the computation cost in the analysis of multipass welds. Several weld passes were assumed as one lumped pass in this model. Recommendations were provided about ramp lumping techniques and the optimum number of weld passes that can be combined into a single thermal input.

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Comparison of Biological Activities of Epimedium koreanum Nakai Produced in Korea and China (국내산과 중국산 삼지구엽초의 생리활성 비교)

  • Noh, Joon-Hyun;Kim, Young-Jin;Kim, Se-Won;Lee, Jin-Ha;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.3
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    • pp.195-200
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    • 2003
  • This study was carried out to compare the biological activities of Epjmedium koreanum Nakai grown wild in Korea and China. The antioxidative effect of E. koreanum Nakai-extracts grown wild in Gangwondo was 78%, which was higher than that in China as 71%. The inhibition ratio of growing cancer cells was estimated as 89, 91 and 86% for human liver, lung and breast cancer cell lines, respectively in adding 0.1 g/l of ethanol extracts of E. koreanum grown in Korea. The hepatoprotective effect and Angiotensin Converting Enzyme (ACE) inhibiting effect of Korean one were also observed as 124 and 87%, respectively, which were also higher than those produced in China. There was not much difference between Korean and Chinese one in inhibiting ${\alpha}-glucosidase$ activities in all ranges of supplement. It was proved that most effective extraction solvent was mixed type as water and ethanol (1:1, v/v) to have higher biological activities from both Korean and Chinese ones.

Fabrication of SiCp/Al Alloy Composites by In-situ Vacuum Hot Press Process (In-situ Vacuum Hot Press 공정을 이용한 SiCp/Al 복합재료의 제조)

  • Choe, Se-Won;Hong, Seong-Gil;Kim, Yeong-Man;Jang, Si-Yeong;Gang, Chang-Seok
    • Korean Journal of Materials Research
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    • v.11 no.7
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    • pp.590-598
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    • 2001
  • SiCp/pure Al and SiCp/2024Al MMCs were fabricated by in-situ VHP process designed specially just in this study which is composed of the vacuum hot press at range from R.T. to $500^{\circ}C$ and the continuous extrusion without canning process at $520^{\circ}C$. It was investigated the effect of SiC particle size, volume fraction and extrusion ratio on the tensile properties and micro structure in auf composites. In case of the 10:1 extrusion ratio, but SiCp/pure Al and SiCp/2024Al composites were shown a sound appearance and a good micro structure without crack of SiCp as well as uniform distribution of SiCp. However, in case of the 16:1 extrusion ratio, the number of cracked SiC particles more than increased in a higher volume fraction composite and 2024Al matrix composite compared with pure Al matrix one. The tensile strength of the composites reinforced smaller SiCp was higher than that of the bigger SiCp reinforced in same volume fraction and extrusion ratio.

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The Performance-based Executive Stock Options and Firm Value (성과연동형 스톡옵션 부여와 기업가치 : 한국 금융업을 대상으로)

  • Kim, Soo-Jung;Sul, Won-Sik
    • The Korean Journal of Financial Management
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    • v.27 no.2
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    • pp.85-114
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    • 2010
  • Using the financial institutions that have adopted performance-based stock option plan, this paper examines whether performance-based executive stock options improves effectively firm value. Over the period 2002~2005, we investigate short-term and long-term effects of the performance-based stock options on stock price. The empirical results are summarized as follows. First, the announcement of plain vanilla stock options generates no significant effects on firm value. Meanwhile, the announcement of performance-based stock options results in negative and significant abnormal returns, which is contrary to the expectation. In addition, we find that there are strong, significant and negative announcement effects when banks grant performance-based stock options. Secondly, there is no significant difference between the long-term performance of the sample granting stock options and that of the benchmarks, which is similar to the findings of the previous research. Also, we fail to get any evidence that performance-based stock option awards have improved the long-term firm value.

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A Study on the Traffic Stream and Navigational Characteristics at the Adjacent Sea Area of Busan Central Wharf (부산 중앙부두 주변해역의 교통흐름 및 통항특성에 관한 연구)

  • Kim Se-Won;Lee Yun-Sok;Park Young-Soo;Kim Jong-Sung;Yun Gwi-Ho;Kim Dae-Hee
    • Journal of Navigation and Port Research
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    • v.30 no.1 s.107
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    • pp.9-15
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    • 2006
  • At the adjacent sea area of Busan Central Wharf, a variety of vessels, such as middle-large passenger ships, small hugh speed crafts, container ships, cargo ships and working boats as well as small miscellaneous vessels are freely sailing comparatively without special rules and marine traffic control. In this research, we analyzed traffic stream and navigational characteristics cf main traffic route based on statistics and distribution of tracks by ship's type and tonnage cf the passing vessels after conducting marine traffic survey twice using exclusive software. We examined the traffic safety of the passing vessels by classifying the sea area by each function based on the analysis about this traffic situation, and analyzing the effect by designating 'Inner passage'. We also studied the plan for the effective rearrangement cf Central Wharf considering basically the traffic safety of arrival and departure in a point of view of navigators.

A Study on Temperature Characteristics of Various Depth using Infrared Thermography (적외선 열화상기법을 이용한 균열 깊이에 따른 온도특성에 관한 연구)

  • Jung, Ju-Yeong;Yoon, Hyuk-Jin;Cho, Hyun-Woo;Yang, Hui-Seung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.3
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    • pp.83-89
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    • 2018
  • The thermal characteristics of concrete specimens were analyzed using cement paste specimens with artificial cracks. In order to understand the temperature characteristics of the specimen depending on the crack depth, the specimen was heated and the minimum temperatures of the specimens at which cracks appear were investigated according to the crack depth. It was confirmed that the surface temperature distribution of the specimen varies depending on whether the specimen is cracked or not, because of the single and multiple reflections of the incident energy. Furthermore, as the temperature distribution of the specimen reaches a steady state, the temperature data tends to decrease with the crack depth. Through the observation of the normalized temperatures, it was found that the temperature of the specimens obtained from this experiment reached a steady state after 10 minutes. At this time, the standard deviation of the normalized temperature is around 0.01 or less, and the temperature decreases linearly with increasing crack depth. This result is considered to be closely related to the area where multiple reflections occur in the cracked region. If the correlation between the crack region and the incident energy is analyzed for various specimens, it can be applied to the diffuse reflection of the light.

A Study on the Thermal Characteristics of Horizontal Ground Heat Exchanger using Thermal Response Test (열응답시험을 이용한 수평형 지중열교환기 열특성 연구)

  • Chang, Keun Sun;Kim, Min-Jun;Kim, Young-Jae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.3
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    • pp.24-30
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    • 2016
  • Vertical and standing column well ground heat exchangers have mostly been installed for ground source heat pump systems (GSHP) and thermal response tests (TRT) have been applied to evaluate the thermal characteristics for these heat exchangers. In this paper, the TRT coupled with a line source method was applied to evaluate the thermal characteristics of the horizontal ground heat exchanger (HGHX). Load tests of a HGHX were also performed to examine the daily variations of the ground and fluid temperatures associated with the daily intermittent operation of GSHP. For this test, the straight HGHX (depth 2 m, length 50 m, 8 line) was installed in Ansan city. The results showed that the variations of ground thermal conductivity of HGHX during one year were relatively small with the range of $1.43{\sim}1.64W/m{\cdot}K$, and the maximum and minimum values appeared in December and May, respectively. Load tests with heat injection rate of 6.0 kW for 10 hours per day to HGHX during twelve days were performed in June, September and December, and resulted in a ground initial temperature rise of $4.31^{\circ}C$, $3.14^{\circ}C$, and $1.21^{\circ}C$ during these days, respectively.