• Development and Reproduction
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• v.12 no.3
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• pp.289-296
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• 2008

BPES (Blepharophimosis/Ptosis/Epicanthus inversus Syndrome) is an autosomal dominant disorder caused by mutations in FOXL2. Affected individuals have premature ovarian failure (POF) in addition to small palpebral fissures, drooping eyelids, and broad nasal bridge. FOXL2 is a member of the forkhead family transcription factors. In FOXL2-deficient ovaries, granulosa cell differentiation dose not progress, leading to arrest of folliculogenesis and oocytes atresia. Using yeast two-hybrid screening of rat ovarian cDNA library with FOXL2 as bait, we found that small ubiquitin-related modifier (SUMO)-conjugating E2 enzyme UBE2I protein interacted with FOXL2 protein. UBE2I also known as UBC9 is an essential protein for processing SUMO modification. Sumoylation is a form of post-translational modification involved in diverse signaling pathways including the regulation of transcriptional activities of many transcriptional factors. In the present study, we confirmed the protein-protein interaction between FOXL2 and UBE2I in human cells, 293T, by in vivo immunoprecipitation. In addition, we generated truncated FOXL2 mutants and identified the region of FOXL2 required for its association with UBE2I using yeast-two hybrid system. Therefore, the identification of UBE2I as an interacting protein of FOXL2 further suggests a presence of novel regulatory mechanism of FOXL2 by sumoylation.

• Korean Journal of Health Education and Promotion
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• v.2 no.1
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• pp.31-61
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• 2000

Introduction. Despite the fact that half of premature deaths are caused by unhealthy lifestyles such as smoking tobacco, sedentary lifestyle, alcohol and drug abuse and poor nutrition, there are no theoretical models which accurately explain these health promotion related behaviors. This study tests a new model of health behavior called the Model of Health Promotion Behavior. This model draws on elements and frameworks suggested by the Health Belief Model, Social Cognitive Theory, the Theory of Planned Action and the Health Promotion Model. This model is intended as a general model of behavior but this first test of the model uses amount of exercise as the outcome behavior. Design. This study utilized a cross sectional mail-out, mail-back survey design to determine the elements within the model that best explained intentions to exercise and those that best explained amount of exercise. A follow-up questionnaire was mailed to all respondents to the first questionnaire about 10 months after the initial survey. A pretest was conducted to refine the questionnaire and a pilot study to test the protocols and assumptions used to calculate the required sample size. Sample. The sample was drawn from 2000 eligible participants at two blue collar (utility company and part of a hospital) and two white collar (bank and pharmaceutical) companies located in Southeastern Michigan. Both white collar site had employee fitness centers and all four sites offered health promotion programs. In the first survey, 982 responses were received (49.1％) after two mailings to non-respondents and one additional mailing to secure answers to missing data, with 845 usable cases for the analyzing current intentions and 918 usable cases for the explaining of amount of current exercise analysis. In the follow-up survey, questionnaires were mailed to the 982 employees who responded to the initial survey. After one follow-up mailing to non-respondents, and one mailing to secure answers to missing data, 697 (71.0％) responses were received, with 627 (63.8％) usable cases to predict intentions and 673 (68.5％) usable cases to predict amount of exercise. Measures. The questionnaire in the initial survey had 15 scales and 134 items; these scales measured each of the variables in the model. Thirteen of the scales were drawn from the literature, all had Cronbach's alpha scores above .74 and all but three had scores above .80. The questionnaire in the second mailing had only 10 items, and measured only outcome variables. Analysis. The analysis included calculation of scale scores, Cronbach's alpha, zero order correlations, and factor analysis, ordinary least square analysis, hierarchical tests of interaction terms and path analysis, and comparisons of results based on a random split of the data and splits based on gender and employer site. The power of the regression analysis was .99 at the .01 significance level for the model as a whole. Results. Self efficacy and Non-Health Benefits emerged as the most powerful predictors of Intentions to exercise, together explaining approximately 19％ of the variance in future Intentions. Intentions, and the interaction of Intentions with Barriers, with Support of Friends, and with Self Efficacy were the most consistent predictors of amount of future exercise, together explaining 38％ of the variance. With the inclusion of Prior Exercise History the model explained 52％ of the variance in amount of exercise 10 months later. There were very few differences in the variables that emerged as important predictors of intentions or exercise in the different employer sites or between males and females. Discussion. This new model is viable in predicting intentions to exercise and amount of exercise, both in absolute terms and when compared to existing models.

• Proceedings of the Mineralogical Society of Korea Conference
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• 1996.10a
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• pp.7-24
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• 1996

'90년대 전반기 중 세계 경제 상황은 정치, 경제 및 환경의 급격한 변화에 따른 불황으로 유황시장에도 심대한 영향을 미치게 되었다. 이와 같은 경기의 후퇴는 다수의 생산자로 하여금 전반적인 시장 상황을 바꿀 수 있는 전략 변경 및 새로운 시장 개발을 촉진시키게 하였다. 최근에 전개되고 있는 극단적인 정치$\cdot$경제적인 변화는 재래시장과 무역균형을 변경시키게 되었다. 개선되는 세계경제에 따른 광범위한 구조조정과 지난 2년간 진행되어온 전반적으로 농업에 유리한 조건에 힘입어 1995년 유황공업은 회복이 시작되어 54.63백만 톤이 생산되었다 국제 유황 무역은 수요와 공급 면에서는 균형을 못 찾고 있다. 1995년 유황생산 중 무역량은 $45\%$에 이르렀다. 유황생산에 영향하는 요소가 변함에 따라 무역의 형태가 변하게 되고 세계 유황 수급 균형에 영향을 주게 되었다. 지난 2년간 있었던 유황생산 회복은 다음 십년간 지속될 것으로 예측된다. 대부분 수요의 증가는 자연가스의 생산을 지배하는 요소에 따라 결정 될 것이다 1986년 이래 유가가 떨어진 후 세계 에너지 수요는 연간 $2.3\%$ 증가하였다. 석유와 가스는 미래 에너지 수요의 증가로 $70\%$이상을 공급하게 될 것이다. 동아시아에서 회수 유황은 원유의 정제로부터 유래된 것으로 가장 신장이 큰 공급원으로 2005년까지 주요유황생산 부분이 될 것이다. 동아시아에서 주요 회수 유황생산국은 일본으로 전체의 $66\%$를 차지한다. 석유 정제로부터 회수 유황생산량은 동아시아에서 증가하고 있다. 유황회수 시설 투자는 일본을 위시하여 한국, 싱가폴 및 태국에서 이루어지고 있으며 이는 점차 증대되는 환경규제에 기인된다. 동아시아 공업국가 예컨대 일본과 한국에서의 유황소비는 인산생산 저조로 정체해 있으나 지난 10년간 여타의 아시아 국가에서의 유황소비는 꾸준히 증가되었다. 이 같은 증가는 앞으로 10년간 계속 될 것으로 추정된다. 이는 유황비료 소비가 4.81백만 톤에서 6.6백만 톤으로 증가될 것으로 예상되고 이는 주로 중국이 내수 인산생산을 증가시키려는 데 기인된다. 더욱이 다가올 10년은 다수의 아시아 국가의 급속한 경제 발전으로 비료 이외의 유황의 소비가 꾸준히 증가 될 것이다. 동아시아는 10.29백만 톤을 생산하고 10.99백만 톤을 소비하여 1995년에는 70만 톤의 유황이 부족하였다. 이와 같은 영향이 계속된다면 동아시아 유황부족은 2005년에는 1.05백만 톤으로 증가될 것으로 예상된다. 그러나 중국에서 황화철에서부터 공급되는 유황함량이 많아 이 지역에서의 원소유황의 진정한 균형에 대해서는 정확하게 평가되지 못한다. 1995년 동아시아에서는 1.3백만 톤의 원소유황을 일본, 캐나다, 미국에서 수입했다. 이들 국가는 앞으로도 이 지역의 주요 공급자가 될 것이다. 황산의 많은 양은 일본에서 이 지역으로 수출되는데 그 양은 1995년 10만톤 이상에 이른다. 더욱이 경제?환경적 이점 때문에 중국이 황화물에서 회수하는 유황대신 원소 유황의 수입을 지속적으로 증가시키고 있어 지역내의 유황 부족이 증가 될 것이다. 이 같은 상황진전으로 앞으로 10년 이내에 2.5백만 톤의 추가시장이 있게 될 것이다. 이 기간내 한국으로서 현재 326,000톤의 부족에서 2005년에는 309,000톤의 과잉으로 유황균형이 변할 수 있는 주요계기가 될 것이다 이 같은 과잉은 회수 유황생산이 1995년 333,000톤에서 2005년 870,000톤으로 $161\%$가 증가될 것으로 예상된다. 동기간 내에 기타 유황생산은 280,000톤에서 320,000톤으로 $14\%$ 증가되는 것으로 추정된다. 그리하여 2005년 한국에서 유황 공급은 1.19백만 톤이고 수요는 881,000톤으로 추정 된다. 미래 한국에서 유황의 또 다른 잠재시장은 식물양분으로서 이다. 인산비료 생산은 유황산업의 골격으로 1995년 세계적으로 인산비료는 유황소비의 53%인 53.60백만 톤을 점유하였다. 작물의 유황결핍 현상은 세계도처에서 나타나고 있어 식물양분으로서 유황시장은 20년전의 시장과 같이 현황을 띠는 시장으로 유황공업이 때를 만나게 될 것이다. 공업국에서 유황의 대기로의 방출억제로 자연 공급량이 감소되고 개발도상국에서 증산으로 유황의 탈취가 증가됨에 따라 유황 부족은 점차 중요한 문제로 확산되고 있다. 세계적으로 $1993\~1994$년간 7.52백만 톤으로 추정되는 유황 부족이 농산물의 수량과 질을 하락시키는 결과를 가져오게 하였다: 이와 같은 현상으로 유황비료의 수요가 증대되었고 산업계는 수요증대에 대응할 기술개발에 노력하게 되었다. 현재의 식량생산과 비료 소비추세가 지속된다면 아시아에서 2000년까지 매년 4.5백만 톤의 유황부족이 있게 될 것이다 이와 같은 유황비료의 부족은 적절한 대책을 취하지 않는 한 2010년에는 6.5백만 톤으로 증가 될 것이다. 동아시아는 경제발전으로 유황비료 장기 잠재시장이 기대되고 새로운 시장으로 $50\%$이상을 점유하게 될 것이다. 서구와 북미에서 유황비료 산업은 이윤 있는 잠재시장으로 인정되고 상업적으로 앞서 있는 시장이다. 점증하는 수요에 대한 대처와 유황비료의 성공은 시장에서 가격에 좌우된다. 실제로 북미와 서구에서 현재의 소매가격은 유황 톤당 $266\~466\$$의 범위에 있다. 인도에서는 비료로서 유황시장은 덜 발달된 단계로서 대표가격은 $120\$$이다. 이 가격 범위로 보아 2010년에 동아시아 시장의 잠재 유황비료 시장은 3.4백만 톤에 이르고 비료공업에서 추가로 얻는 이윤은 408백만 내지 1조5천억$\$$이 될 것이다. 이와 같은 시장이 발전 될 수 있는 것은 계속된 제품개발과 비료산업 시장개척에 달려있다.

• Journal of the Korean Society of Radiology
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• v.17 no.2
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• pp.257-265
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• 2023

The purpose of this study was to vaccinate every year according to the general characteristics of COVID-19, whether to vaccinate every year according to the vaccination experience, whether to vaccinate every year according to knowledge/attitude about vaccination, and negative responses to the vaccinate every year In order to understand the factors affecting the vaccination physician every year by identifying the factors of Statistical analysis is based on general characteristics, variables based on vaccination experience, and knowledge/attitudes related to vaccination. The doctor calculates the frequency and percentage, A square test (-test) was performed, and if the chi-square test was significant but the expected frequency was less than 5 for 25% or more, a ratio difference test was performed with Fisher's exact test. Through multiple logistic regression analysis using variables that were significant in simple analysis, a predictive model for future vaccination and the effect size of each independent variable were estimated. As statistical analysis software, SAS 9.4 (SAS Institute Inc., Cary, NC, USA) was used, and because the sample size was not large, the significance level was set at 10%, and when the p-value was less than 0.10, it was interpreted as statistically significant. In the simple logistic regression analysis, the reason why they answered that they would not be vaccinated every year was that they answered 'to prevent infection of family and hospital guests' rather than 'to prevent my infection' as the reason for the vaccination. It was 11.0 times higher and 3.67 times higher in the case of 'for the formation of collective immunity of the local community and the country'. The adverse reactions experienced after the 1st and 2nd vaccination were 8.42 times higher in those who did not experience pain at the injection site than those who did not, 4.00 times higher in those who experienced swelling or redness, and 5.69 times higher in those who experienced joint pain. There was a 5.57 times higher rate of absenteeism annually than those who did not. In addition, the more anxious they felt about vaccination, the more likely they were to not get the vaccine every year by 2.94 times.

• Journal of Mushroom
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• v.21 no.3
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• pp.132-139
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• 2023

Light plays an important role in fruit-body development and morphology during Pleurotus spp. cultivation. To understand the effects of light color on fruit-body properties, we evaluated the fruit-body characteristics of Pleurotus spp. Varieties cultivated under blue, red, and purple LED light sources. The main results are as follows: The overall fruit-body shape showed differences depending on the color of the LED light. The fruit-bodies of mushroom cultivated under blue and purple light were generally similar to the mushroom shapes typically produced, while those of mushroom cultivated under green light were abnormally shaped, probably due to the absence of effective light source. The average cap lightness of mushrooms cultivated under blue, green, and purple LED lights was 57.0, 57.4, and 59.4, respectively. The average cap lightness of all varieties except Wonhyeong1ho and Hwang-geumsantari cultivated under the three LED light sources were statistically significantly different (P<0.05). The cap redness varied significantly depending on the LED lighting and variety. Only Gonji7hoM, the cap color mutant of Gonji7ho, showed negative cap redness values under all three LED light sources. Among the eight varieties excluding Gonji7ho, the highest cap redness was observed when cultivated under the blue LED. The average harvest weight of the varieties cultivated under purple, blue, and green LED light were 68.0, 58.3, and 50.1 g, respectively. The yield of Gonji7ho, the mushroom variety with the highest yield, cultivated under blue, green, and purple LED light were 92.8, 77.1, and 98.6 g, respectively. The earliness when grown under the purple, blue, and green LED lights were 5.3, 5.8, and 5.8 days, respectively. Among the varieties, six, three, and two cultivars showed the shortest earliness under the purple, green, and blue LED, respectively. The fruit-body lengths were 66.4, 51.8, and 46.8 mm when cultivated under green, purple, and blue lights, respectively. These results are expected to serve as a foundation for producing mushrooms with traits demanded in the market.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.9 no.1
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• pp.9-16
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• 2023

Liposomes as drug delivery system have proved useful carrier for various disease, including cancer. In addition, perfluorocarbon cored microbubbles are utilized in conjunction with high-intensity focused-ultrasound (HIFU) to enable simultaneous diagnosis and treatment. However, microbubbles generally exhibit lower drug loading efficiency, so the need for the development of a novel liposome-based drug delivery material that can efficiently load and deliver drugs to targeted areas via HIFU. This study aims to develop a liposome-based drug delivery material by introducing a substance that can burst liposomes using ultrasound energy and confirm the ability to target tumors using PET imaging. Liposomes (Lipo-DOX, Lipo-DOX-Au, Lipo-DOX-Au-RGD) were synthesized with gold nanoparticles using an avidin-biotin bond, and doxorubicin was mounted inside by pH gradient method. The size distribution was measured by DLS, and encapsulation efficiency of doxorubicin was analyzed by UV-vis spectrometer. The target specificity and cytotoxicity of liposomes were assessed in vitro by glioblastoma U87mg cells to HIFU treatment and analyzed using CCK-8 assay, and fluorescence microscopy at 6-hour intervals for up to 24 hours. For the in vivo study, U87mg model mouse were injected intravenously with 1.48 MBq of ⁶⁴Cu-labeled Lipo-DOX-Au and Lipo-DOX-Au-RGD, and PET images were taken at 0, 2, 4, 8, and 24 hours. As a result, the size of liposomes was 108.3 ± 5.0 nm at Lipo-DOX-Au and 94.1 ± 12.2 nm at Lipo-DOX-Au-RGD, and it was observed that doxorubicin was mounted inside the liposome up to 52%. After 6 hours of HIFU treatment, the viability of U87mg cells treated with Lipo-DOX-Au decreased by around 20% compared to Lipo-DOX, and Lipo-DOX-Au-RGD had a higher uptake rate than Lipo-DOX. In vivo study using PET images, it was confirmed that ⁶⁴Cu-Lipo-DOX-Au-RGD was taken up into the tumor immediately after injection and maintained for up to 4 hours. In this study, drugs released from liposomes-gold nanoparticles via ultrasound and RGD targeting were confirmed by non-invasive imaging. In cell-level experiments, HIFU treatment of gold nanoparticle-coupled liposomes significantly decreased tumor survival, while RGD-liposomes exhibited high tumor targeting and rapid release in vivo imaging. It is expected that the combination of these models with ultrasound is served as an effective drug delivery material with therapeutic outcomes.

• Journal of the Korean Society of Radiology
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• v.17 no.4
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• pp.489-495
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• 2023

Radiation shielding facilities are constructed in locations where diagnostic radiation generators are installed, with the aim of preventing exposure for patients and radiation workers. The purpose of this study is seek to compare and validate the trend of attenuation thickness of lead, the primary material in these radiation shielding facilities, at different maximum tube voltages by Monte Carlo simulations and measurement. We employed the Monte Carlo N-Particle 6 simulation code. Within this simulation, we set a lead shielding arrangement, where the distance between the source and the lead sheet was set at 100 cm and the field of view was set at 10 × 10 cm². Additionally, we varied the tube voltages to encompass 80, 100, 120, and 140 kVp. We calculated energy spectra for each respective tube voltage and applied them in the simulations. Lead thicknesses corresponding to attenuation rates of 50, 70, 90, and 95% were determined for tube voltages of 80, 100, 120, and 140 kVp. For 80 kVp, the calculated thicknesses for these attenuation rates were 0.03, 0.08, 0.21, and 0.33 mm, respectively. For 100 kVp, the values were 0.05, 0.12, 0.30, and 0.50 mm. Similarly, for 120 kVp, they were 0.06, 0.14, 0.38, and 0.56 mm. Lastly, at 140 kVp, the corresponding thicknesses were 0.08, 0.16, 0.42, and 0.61 mm. Measurements were conducted to validate the calculated lead thicknesses. The radiation generator employed was the GE Healthcare Discovery XR 656, and the dosimeter used was the IBA MagicMax. The experimental results showed that at 80 kVp, the attenuation rates for different thicknesses were 43.56, 70.33, 89.85, and 93.05%, respectively. Similarly, at 100 kVp, the rates were 52.49, 72.26, 86.31, and 92.17%. For 120 kVp, the attenuation rates were 48.26, 71.18, 87.30, and 91.56%. Lastly, at 140 kVp, they were measured 50.45, 68.75, 89.95, and 91.65%. Upon comparing the simulation and experimental results, it was confirmed that the differences between the two values were within an average of approximately 3%. These research findings serve to validate the reliability of Monte Carlo simulations and could be employed as fundamental data for future radiation shielding facility construction.

• Journal of Practical Agriculture & Fisheries Research
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• v.16 no.1
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• pp.193-206
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• 2014

The objectives of the project are to increase farmers' income through GAP and to reduce the loss of agricultural produce, for which the Korean partner takes a role of transferring needed technologies to the project site. To accomplish the project plan, it is set to implement the project with six components: construction of buildings, installation of agricultural facilities, establishment of demonstration farms, dispatching experts, conducting training program in Korea and provision of equipments. The Project Management Committee and the Project Implementation Team are consisted of Korean experts and senior officials from Department of Agriculture, Myanmar that managed the project systematically to ensure the success of the project. The process of the project are; the ceremony of laying the foundation and commencing the construction of training center in April, 2012. The Ribbon Cutting Ceremony for the completion of GAP Training Center was successfully held under PMC (MOAI, GAPI/ARDC) arrangement in SAl, Naypyitaw on June 17, 2012. The Chairman of GAPI, Dr. Sang Mu Lee, Director General U Kyaw Win of DOA, officials and staff members from Korea and Myanmar, teachers and students from SAl attended the ceremony. The team carried out an inspection and fixing donors' plates on donated project machineries, agro-equipments, vehicles, computers and printer, furniture, tools and so forth. Demonstration farm for paddy rice, fruits and vegetables was laid out in April, 2012. Twenty nine Korean rice varieties and many Korean vegetable varieties were introduced into GAP Project farm to check the suitability of the varieties under Myanmar growing conditions. Paddy was cultivated three times in DAR and twice in SAl. In June 2012, vinyl houses were started to be constructed for raising seedlings and finished in December 2012. Fruit orchard for mango, longan and dragon fruit was established in June, 2012. Vegetables were grown until successful harvest and the harvested produce was used for panel testing and distribution in January 2013. Machineries for postharvest handling systems were imported in November 2012. Setting the washing line for vegetables were finished and the system as run for testing in June 2013. New water tanks, pine lines, pump house and electricity were set up in October 2013.

• Journal of Practical Agriculture & Fisheries Research
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• v.4 no.1
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• pp.128-137
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• 2002

This study was conducted to develop the heat exchanger by utilizing the heat energy of underground water(15℃), which might be used for cooling and heating system of the agricultural facilities. We developed the heat exchanger by using the parallel type plat fin tube made of Aluminum(Al 6063), which was named Aloo-Heat(No. 0247164, offered by Korean Intellectual property Office). The trial manufactures were made from Aloo-heat which was 600mm, 700mm length respectively, and It were welded to the end "U" type in order to direct flow of the underground water. The performance test was carried out under the condition of open space and room temperature with the change of flow rate of the underground water and air. The results are as follows. 1. The trial manufactures had convection heat value from 33 to 156 W/m²℃, and It was coincided with design assumption. 2. The amount of energy transfer was increased with the increment of the area of heat transfer, the air flow, the gap of temperature inlet & outlet the underground water and the air. 3. The heat value was 6,825W when the air flow was 6,000m³/h and the gap of temperature between inlet and outlet of the underground water was 6℃, and It dropped from 25.8℃ to 23.2℃(-2.6℃ difference). The convection heat value was 88.5W/m²℃. 4. The heat value was 2.625W when the air flow was 4,000m³/h and the gap of temperature between inlet and outlet the underground water was 2℃, and It dropped from 27℃ to 22.5℃(-4.5℃ difference). The convection heat value was 33.6W/m²℃. 5. Correlation values(R²) of the testing heat values of the trial manufacture type I, II, and III were 0.9141, 0.8935, and 0.9323 respectively, and correlation values(R²) of the amount of the air flow 6,000m³/h, 5,000m³/h, 4,000m³/h were 0.9513, 0.9414, and 0.9003 respectively.

• Journal of Practical Agriculture & Fisheries Research
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• v.4 no.1
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• pp.119-127
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• 2002

This study was conducted to develop the heat exchanger by utilizing the heat energy of underground water(15℃), which might be used for cooling and heating system of the agricultural facilities. We developed the heat exchanger, parallel type plat fin tube made of Aluminum(Al 6063), which was named Aloo-Heat(No. of The registration design : 0247164, by Korean Intellectual property Office). The fin of exchanger was design of the granulated surface for minimizing fouling factor and dew forms, and also placed parallel to the tube in order to minimized the resistance of flows. 1. Aloo-heat was designed to have 0.03m for inside diameter, 0.036m for outside diameter of tube, 0.0012m for thickness of fin and 0.032m for length of plat fin. 2. t was also designed to have 1.5248m²/m for outside area of heat transfer, 0.0942m²/m for inside area contacting hot liquid, and the ratio (Ra) was 16.1869. 3. Efficiency of the fin was 93 percentage when fin length was 0.032m, and the fin thickness satisfied equation $\frac{h{\rho}}{k}$< 0.2 when it was 0.0012m. 4. According to the performance test of Aloo-heat, as the temperature and rate increased, the heating value also increased, heating value was 504kJ/h·m and 6,048kJ/h·m when it was 60℃, 10 𝑙/min and 80℃, 40 𝑙/min respectively. 5. The test of heating value was confident, because correlation value(R²) was 0.9898 for the temperature and 0.9721 for flow rate of hot liquid, respectively.