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Manure Based Duckweed Production in Shallow Sink : Effect of Genera on Biomass and Nutrient Yield of Duckweed under the Same Nutritional and Management Conditions

  • Chowdhury, S.A.;Sultana, N.;Huque, K.S.;Huque, Q.M.E.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.5
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    • pp.686-693
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    • 2000
  • Biomass yield, nutritive value and nutrient utilization efficiency of different genera of duckweed (DW, Lemnaceae) under the same nutritional and management conditions were studied. Three genera of DW namely: Lemna perpusilla, Spirodela polyrhiza and Woljfia arrhiza, were cultivated in 18 plastic sinks each of $59{\times}54{\times}35cm^3$ size. Each sink contained approximately 80 L of water and was charged once with 6.648 kg of anaerobically fermented cow dung effluent. The seed rate for Spirodela, Lemna and Wolffia were 600, 400 and $600g/m^2$ respectively. Duckweed were harvested at 48 hours intervals. Media total N concentration for Lemna and Wolffia gradually increased with time. Growth of duckweed was measured by subtracting the inoculum from the total biomass production. Growth of Spirodela ceased within 4 days but Lemna and Wolffia continue to grow up to 34 days. Bio-mass yield was significantly (p<0.05) higher in Wolffia (906 kg/ha/d) than Lemna (631 kg/ha/d). Dry matter (DM) yield was very similar in Lemna (14.80 kg/ha/d) and Wolffia (14.57 kg/ha/d). The N content was non-significant higher in Lemna (5.45%) than Wolffia (5.00%) and Spirodela (4.6%). The crude protein (CP, $N{\times}6.25$) yield was non-significantly higher in Lemna (4.83 kg/ha/d) than Wolffia (4.32 kg/ha/d). The acid detergent fibre (ADF) content was the highest in Wolffia (28.59%), followed by Spirodela (19.47%) and Lemna (12.39%). Utilization efficiency of CP was 273 and 314% respectively for Wolffia and Lemna. However, similar efficiency values for Spirodela was only 1.5%. Considering the bio-mass yield, nutritive value and nutrient utilization efficiency, production performance of DW were in the order of Lemna>Wolffia>Spirodela under the present experimental conditions.

A Study for Evaluating of Voltage Stability Margin Considering Shunt Capacitor (조상설비를 고려한 전압안정성 여유전력의 평가에 관한 연구)

  • 김세영
    • Journal of Energy Engineering
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    • v.7 no.1
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    • pp.65-72
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    • 1998
  • This paper presents a fast calculation method for evaluating of voltage stability margin (MW) using the line flow equation in polar form. Here, Line flow equations $(P_{ij},\;Q_{ij}$ are comprised of state variable, $V_i,\;{\Delta}_i,\;V_j$ and ${Delta}_j$, and line parameter, r and x. using the feature of polar coordinate, these becomes one equation with two variables, $V_j,;V_j$. Moreover, if bus j is slack or generator bus, which is specified voltage magnitude in load flow calculation, it becomes one equation with one variable $V_ i $ that is, may be formulated with the second-order equation for $V^2_i$. Therefore, multiple load flow solutions may be obtained with simple computation. The obtained load flow multiple solutions are used for evaluating of voltage stability through sensitivity analysis or its closeness. Also, the method is proposed to calculate for voltage stability margin considering shunt capacitor, which is important element for evaluating of voltage stability. The proposed method was validated to sample systems.

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Variation of Undrained Shear Behavior with Consolidation Stress Ratio of Nakdong River Sand (압밀응력비에 따른 낙동강모래의 비배수전단거통 특성)

  • 김영수;정성관;송준혁;정동길
    • Journal of the Korean Geotechnical Society
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    • v.19 no.4
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    • pp.83-93
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    • 2003
  • This research, in order to study the effects of initial shear stress of anisotropically consolidated sand that has 0.558% fines, performed several undrained static and dynamic triaxial test. To simulate the real field conditions, loose and dense samples were prepared. Besides, the cyclic shear strength of Nakdong River sand under various combinations of initial static shear stress, stress path, pore water pressure and residual strength relationship was studied. By using Bolton's theory, peak internal friction angle at failure which has considerable effects on the relative density and mean effective stress was determined. In p'- q diagram, the phase transformation line moves closer to the failure line as the specimen's initial anistropical consolidation stress increases. Loose sands were more affected than dense sands. The increase of consolidation stress ratio from 1.4 to 1.8 had an effect on liquefaction resistance strength resulting from the increase of relativity density, and showed similar CSR values in dense specimen condition.

Ecological Management Plan and Biotope Structure of Namsan Urban Natural Park in Seoul (서울 남산도시자연공원의 비오톱 구조 및 생태적 관리방안)

  • Lee Kyong-Jae;Han Bong-Ho;Lee Soo-Dong
    • Journal of the Korean Institute of Landscape Architecture
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    • v.32 no.5
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    • pp.102-118
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    • 2004
  • The purpose of this study was to propose an ecological management plan by the comprehensive analysis of biotope structures on Namsan Urban Natural Park in Seoul. Classified by actual vegetation, structure of layer and vegetation damage, biotope structures were composed of forest area, compact management area, herb area, cultivated area and non-ecology(urban) area. Succession had seened to stop in the Native forest. Artifical forest was divided into two types. The first, upper layer, was too dense to accommodate lower layer plants, the other case was the appearance of Quercus spp. and the first stage plants of succession following the declination of the upper layer plants. The soil pH of Nam-san Urban Park was 4.21∼4.51, which meant the soil was becoming acid. As the result of acidity, leaching of available nutrition(K/sup +/, NH₄/sup +/, Ca/sup ++/ etc.) was immediately influenced by the natural ecosystem, influence of acid rain was disturbed to becoming organic matter which was use to plants. In the case of a biotope structure management plan, the urban area was prohibited to spread outside. Cultivated and herb area was regenerated to natural forest. In the forest area, the compact management area was maintained with its present condition, and then it is desirable to make a preservation area and to plant shrubs. Planted Pinus densiflora Community was needed to eliminate competitive species of canopy layer, and plant shrubs. Management of deciduous broad-leaved Comm. was maintained in its present conditionand it is desirable to raise the diversity of the understory and shrub layer. The management of the artifical forest seems to be suitable for Q. spp. community. The care of naturalized plants prevents the expansion and restores the structure of wild plants. The soil management was a marked restoration soil ecosystem in order to prevent soil acid and drying.

A Study on Characteristics of Temperature and Radicals in Laminar Non-premixed H2/N2 Flame Using LIPF and LRS (레이저 유도 선해리 형광법과 래일레이 산란법에 의한 층류 비예혼합 수소/질소 화염의 온도 및 라디칼 특성에 관한 연구)

  • Jin, Seong Ho;Park, Kyoung Suk;Kim, Gun Hong;Kim, Gyung Soo
    • Transactions of the Korean hydrogen and new energy society
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    • v.13 no.3
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    • pp.169-180
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    • 2002
  • Rayleigh scattering and laser induced predissociative fluorescence are used to obtain two-dimensional images of temperature and species concentration in a laminar non-premixed flame of a diluted hydrogen jet. Rayleigh scattering cross-sections are experimentally obtained at 248nm. Planar images of OH and $O_2$ with tunable KrF excimer laser which has a) $0.5cm^{-1}$ linewidth, b) 0.5nm tuning range, c) 150mJ pulse energy, and d) 20ns pulse width are obtained to determine spatial distributions of OH and $O_2$. The technique is based on planar laser induced predissociative fluorescence (PLIPF) in which collisional quenching is almost avoided because of the fast predissociation. Dispersed LIPF spectra of OH and $O_2$ are also measured in a flame in order to confirm the excitation of single vibronic state of OH and $O_2$. OH and $O_2$ are excited on the $P_2$(8) and $Q_1$(11) line of the $A^2{\Sigma}^{+}({\nu}^{'}=3)-X^{2}{\Pi}({\nu}^{''}=o)$ band and R(17) line of the Schumann-Runge band $B^{3}{\Sigma}_{u}{^-}(\nu^{'}=0)-X^{3}{\Sigma}_{g}{^-}({\nu}^{''}=6)$, respectively. Fluorescence spectra of OH and Hot $O_2$ are captured and two-dimensional images of the hydrogen flame field are successfully visualized.

Studies on the Structure of the Forest Community in Mt. Sokri(II) -Analysis on the Plant Community by the Classification and Ordination Techniques- (속리산 삼림군집구조에 관한 연구(II) Classification 및 Ordination 방법에 의한 식생분석 -)

  • 이경재;박인협;조재창;오충현
    • Korean Journal of Environment and Ecology
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    • v.4 no.1
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    • pp.33-43
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    • 1990
  • A survey of Popju Temple district. was conducted using 70 sample plots of 500$m^2$ size. The classification by TWINSPAN and DCA ordination were applied to the study area in order to classify them into several groups based on woody plants and environmental variables. By both techniques. the plant com-munity were divided into six groups by the altitude and soil moisture. The successional trends of tree species seem to be from Pinus densiflora, Sorbus alnifolia through Quercus serrata to Carpinus laxiflora and from P. densiflora, Fraxinus sieboldiana through Q. mongolica in the canopy layer, and from Lespedeza cyrtobotrya, Rhus trichocarpa, Zanthoxylum schnifolium through Rhododendron mucronulatum, Corylus sieboldiana, Lindera obtusiloba, Magnclia sieboldii to Euonymus sieboldianus in the understory and shrub layer. The species diversity of the plant community in the burnt plot was decreased by the forest fire but the importance values of Quercus species were increased in above plot.

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Energy Efficient Alloy Design in PSN-PMN-PZT Ceramic System for Piezoelectric Transformer Application (고효율 압전 트랜스포머용 PSN-PMN-PZT 조성 설계)

  • Choi Yong-Gil;Ur Soon-Chul;Yoon Man-Soon
    • Korean Journal of Materials Research
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    • v.15 no.12
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    • pp.814-817
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    • 2005
  • In order to enhance energy efficiency in high electric conversion devices such as Power transformers, which need to have high power properties, an alloy design approach in $Pb(Zr,Ti)O_3(PZT)$ base ceramic system was attempted $0.03Pb(Sb_{0.5}Nb_{0.5})O_3-0.03Pb(Mn_{1/3}Nb_{2/3})O_3-(0.94-x)PbTiO_3-xPbZrO_3$[PSN-PMN- PZT] ceramics were synthesized by conventional bulk ceramic processing technique. To improve power properties, the various Zr/Ti ratio was varied ]lear their morphotropic phase boundary (MPB) composition of PSN-PMN-PZT system and their effects on subsequent piezoelectric and dielectric properties for the transformer application at high power were systematically investigated using an impedance analyzer. Microstructure and phase information were characterized using X-ray diffractometer (XRD), a scanning electron microscope (SEM) and others. When the Zr/Ti ratio was 0.415/0.465, the value of $Q_m\;and\;k_p$ were shown to reach to the maximum, indicating that this alloy design can be a feasible composition :or high power transformer.

Steam Activated Carbon Preparation Using HTFBR from Biomass and its Adsorption Characteristics

  • Asirvatham, J. Herbert;Gargieya, Nikhar;Paradkar, Manali Sunil;Prakash Kumar, B.G.;Lima Rose, Miranda
    • Carbon letters
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    • v.9 no.3
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    • pp.203-209
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    • 2008
  • The objective of this work is to study the feasibility of the preparation of the activated carbon (AC) from coconut tree flowers using high temperature fluidized bed reactor (HTFBR). The activating agent used in this work is steam. The reactor was operated at various activation temperature (650, 700, 750, 800 and $850^{\circ}C$) and activation time (30, 60, 120 and 240 min) for the production of AC from coconut tree flowers. Effect of activation time and activation temperature on the quality of the AC preparation was observed. Prepared AC was characterized in-terms of iodine number, methylene blue number, methyl violet number, ethylene glycol mono ethyl ether (EGME) surface area and SEM photographs. The best quality of AC from coconut tree flowers (CFC) was obtained at an activation temperature and time of $850^{\circ}C$ and 1 hr restectively. The effectiveness of carbon prepared from coconut tree flowers in adsorbing crystal violet from aqueous solution has been studied as a function of agitation time, carbon dosage, and pH. The adsorption of crystal violet onto AC followed second order kinetic model. Adsorption data were modeled using both Langmuir and Freundlich classical adsorption isotherms. The adsorption capacity $q_m$ was 277.78 mg/g., equilibrium time was found to be 180 min. This adsorbent from coconut tree flowers was found to be effective for the removal of CV dye.

Enhancement of anti-inflammatory and anti-tumorigenic properties of 3D-spheroid formed mesenchymal stem cells derived from rheumatoid arthritis joints

  • Seung-Chan Lee;Chae-Yeon Hong;Yong-Ho Choe;Tae-Seok Kim;Won-Jae Lee;Gyu-Jin Rho;Sung-Lim Lee
    • Journal of Animal Reproduction and Biotechnology
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    • v.37 no.4
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    • pp.246-254
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    • 2022
  • Current studies have revealed the capacity of mesenchymal stem cells (MSCs) in term of immunomodulatory properties, and this distinct potential is downgraded according to the disease duration of patients-derived MSCs. In order to enhance the immunomodulatory and anti-tumorigenic properties of the rheumatoid arthritis (RA) joints-derived MSCs, we aggregate synovial fluid-derived MSCs from RA joints (RA-hMSCs) into 3D-spheroids by the use of hanging drop culture method. Cells were isolated from synovial fluids of RA joints with longstanding active status over 13 years. For aggregation of RA-hMSCs into 3D-spheroids, cells were plated in hanging drops in 30 μL of advanced DMEM (ADMEM) containing 25,000-30,000 cells/drop and cultured for 48 h. To analyze the comparative immunomodulatory effects of 3D-spheroid and 2D monolayer cultured RA-hMSCs and then cells were cultured in ADMEM supplemented with 20% of synovial fluids of RA patients for 48 h and were evaluated by qRT-PCR for their expression of mRNA levels of inflammatory and anti-inflammatory markers. Cellular aggregation of RA-hMSCs was observed and cells were aggregate into a single sphere. Following treatment of RA patient's synovial fluids into the RA-hMSCs, spheroids formed RA-hMSCs showed significantly (p < 0.05) higher expression of TNFα stimulated gene/protein 6 (TSG-6) than the monolayer cultured RA-hMSCs. Therefore, the 3D-spheroid culture methods of RA-hMSCs were more effective than 2D monolayer cultures in suppressing inflammatory response treated with 20% of RA-synovial fluids by expression of TNFα (TSG-6) according to the immune response and enhanced secretion of inflammatory factors.

Characterizing Milk Production Related Genes in Holstein Using RNA-seq

  • Seo, Minseok;Lee, Hyun-Jeong;Kim, Kwondo;Caetano-Anolles, Kelsey;Jeong, Jin Young;Park, Sungkwon;Oh, Young Kyun;Cho, Seoae;Kim, Heebal
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.3
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    • pp.343-351
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    • 2016
  • Although the chemical, physical, and nutritional properties of bovine milk have been extensively studied, only a few studies have attempted to characterize milk-synthesizing genes using RNA-seq data. RNA-seq data was collected from 21 Holstein samples, along with group information about milk production ability; milk yield; and protein, fat, and solid contents. Meta-analysis was employed in order to generally characterize genes related to milk production. In addition, we attempted to investigate the relationship between milk related traits, parity, and lactation period. We observed that milk fat is highly correlated with lactation period; this result indicates that this effect should be considered in the model in order to accurately detect milk production related genes. By employing our developed model, 271 genes were significantly (false discovery rate [FDR] adjusted p-value<0.1) detected as milk production related differentially expressed genes. Of these genes, five (albumin, nitric oxide synthase 3, RNA-binding region (RNP1, RRM) containing 3, secreted and transmembrane 1, and serine palmitoyltransferase, small subunit B) were technically validated using quantitative real-time polymerase chain reaction (qRT-PCR) in order to check the accuracy of RNA-seq analysis. Finally, 83 gene ontology biological processes including several blood vessel and mammary gland development related terms, were significantly detected using DAVID gene-set enrichment analysis. From these results, we observed that detected milk production related genes are highly enriched in the circulation system process and mammary gland related biological functions. In addition, we observed that detected genes including caveolin 1, mammary serum amyloid A3.2, lingual antimicrobial peptide, cathelicidin 4 (CATHL4), cathelicidin 6 (CATHL6) have been reported in other species as milk production related gene. For this reason, we concluded that our detected 271 genes would be strong candidates for determining milk production.