• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.447-453
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• 2019

In this study, the effect of supplying volume and frequency of a nutrient solution consisted with $NO_3-N$ 4.6, $NH_4-N$ 3.4, $PO_4-P$ 3, K 3, Ca 4.6 and Mg $2.2mmol{\cdot}L^{-1}$ on growth and fruit quality of 'Duke' blueberry was investigated. Three years old 'Duke' blueberry bushes cultivated in containers ($60{\times}80{\times}40cm$) filled with 130L peat moss and 40L pearlite (v/v) were selected for the experiment. The growth containers were mulched with sawdust. Two different volumes (4L and 8L) of nutrient solution was tested at three different supplying frequencies (one time, two times, and three times) per week and the drainage quality of nutrient solution and fruit quality of 'Duke' blueberry was evaluated. The optimal drainage rate for the vegetable cultivation is known to be 20-30%. The results revealed that the average drainage rates of 27% and 29% for the nutrient solution supplied in 'Duke' blueberry growth medium at 4L, 2 times/7 days and 4L, 3 times/7days, respectively. The highest shoot diameter (4.2mm) and shoot length (31cm) of 'Duke' blueberry was recorded with the 8L of nutrient solution supplied at 3 times per 7 days. According to the analysis of inorganic components in the drainage of nutrient solution, there was a tendency of absorbing nitrogen at the early stage of growth. The supplying volume and frequency of nutrient solution was not significantly affected on 'Duke' blueberry fruit weight, soluble solids content, and titratable acidity. The highest yield per bush (2.7kg) was recorded for the nutrient solution supplied with 4L at three times per 7 days, while the 4L nutrient solution supplied at one time per 7 days resulted the lowest yield of 1.4kg per bush. Consequently, the tested nutrient solution can be applied for the 'Duke' blueberry bushes with the volume of 4L at three times per week for the better crop growth.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.317-330
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• 2009

Research was initiated to investigate germination vigor, number of leaves, plant height and turfgrass density. A total of 18 treatment combinations were used in the study. Treatments were made of soil organic amendment(SOA), sand, and water-absorbing polymer. Germination vigor, leaf number, plant height and turfgrass density were evaluated in Kentucky bluegrass(KB) grown under greenhouse conditions. Significant differences were observed in germination vigor, leaf number, plant height and turfgrass density among 18 mixtures of SOA and polymer. Highest germination rate was associated with mixture of SOA 20% + sand 80% + polymer 0%, resulting in 56.3% for KB. Number of leaves at 60 DAS(days after seeding) were greater with KB over PR, while plant height higher with PR over KB. Leaf number increased with SOA, being SOA 20% > SOA 100% > SOA 10% and with polymer from 0 to 12%. Plant height was greatest with SOA 20% and lowest with SOA 100%. Greater density was observed with PR rather than KB due to longer plant height. Turf density was best under SOA 10% and poorest under SOA 100% in KB. A further research would be required for investigating the individual effect of K-SAM, Ca, perlite on the turf growth characteristics.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.97-102
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• 2005

One of the key molecules involved in skin moisture is hyaluronan (hyaluronic acid, HA) with its associated water of hydration. The predominant component of the ECM (extracellular matrix) of skin is HA. It Is the primordial and the simplest of the GAGs (glycosaminoglycans), a water-sorbed macromolecule In extracellular matrix, Included between the vital cells of epidermis. In the skin, HA was previously thought to derive extlusively from dermis. But, recent studies revealed that HA could be synthesized in epidermis. Flavonoids are polyphenolic compounds that is found mainly in foods of plant origin. Kaempferol was known to increase glutathione synthesis in human keratinocyte. And quercetin blocked PPAR-meidated keratinocyte differentiation as lipoxygenase inhibitors. In this study, we sought to evaluate the effect of flavonid, kaempferol and quercetin on production HA in keratinocyte. We examined the changes of three human hyaluronan synthase genes (HASI, HAS2, HAS3) expression by semi-quantitative RT-PCR when kaempferol or quercetin was added to cultured human keratinocytes. We found that these flavonoids slightly upregulated HAS2, HAS3 mRNA after 24 h. And we investigated the effect on HA production by ELISA. When we evaluated the level of HA in culture medium after 24 h incubation. We found enhanced accumulation of HA in the culture medium. Although the effects of above flavonoids are less than retinoic acid, the data indicate that kaempferol, quercetin can dose-dependently increase the level of HA in epidermis cell line. It suggested that flavonoid, kaempferol, and quercetin increased production of HA in skin and it helped to elevate skin moisture and improve facial wrinkle.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.4 s.54
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• pp.349-357
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• 2005

New antioxidative substances for cosmeceuticals were screened from natural resources such as microbial metabolites, mushrooms, and medicinal plants. Four antioxidants were isolated from the fungal metabolite of Eupenicillium shearii and their structures were determined to be new phenolic compounds. The compounds were designated as melanocins A, B, C, and D. Melanocins $A{\sim}D$ exhibited free radical scavenging activity on DPPH and superoxide with $EC_{50}$ values of $21{\sim}94\;and\;7{\sim}84{\mu}M$, respectively, which were stronger activity than those of ${\alpha}-tocopherol$ and BHA. Melanocin A showed anti-wrinkle effects on the UV-irrated hairless mouse skin. A novel hispidin antioxidative compound designated as inoscavin A was isolated from the fruiting body of the mushroom, Inonotus xeranticus. Inoscavin A scavenged superoxide radical with $EC_{50}$ values of $0.03{\mu}g/mL$, and inhibited rat liver microsomal lipid peroxidation with $EC_{50}$ values of $0.3{\mu}g/mL$. Benzastatins $A{\sim}G$, the novel antioxidants isolated from the culture of Streptomyces nitrosporeus showed potent lipid peroxidation inhibitory activity with $EC_{50}$ values of $3{\sim}30{\mu}M$. A cyclopentene compound with strong hypopigmentary effect was isolated from the fungal metabolite of Penicillium sp. and identifed as terrein. Terrein significantly reduced melanin levels in a melanomacyte cell line, Mel-Ab. It showed 10 times stronger activity than kojic acid, but exhibited no cytotoxic effect even in $100{\mu}M$. It was suggested that terrein reduced melanin synthesis by reducing tyrosinase production by MITF down-regulation.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.26 no.1
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• pp.163-186
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• 2000

We have previously screened 150 medicinal plants on the inhibition of elastase and found a significant inhibitory effects of the extracts of Areca catechu L. on the aging and inflammation against the skin tissues. To isolate and identify the compounds having biological activity, we was further purified by each of the solvent fractions, silica gel column chromatography, preparative TLC and reversed-Phase HPLC. Peak in HPLC, which coincided with the inhibitory activity against elastase, was identified as Phenolic substance using various colorimetric methods, UV, and IR. $IC_{50}$/ values of phenolic substance purified from Areca catechu were 26.9 $\mu\textrm{g}$/$m\ell$ for porcine pancreatic elastase (PPE) and 60.8 $\mu\textrm{g}$/$m\ell$ for human neutrophil elastase (HNE). This Phenolic substance showed more potent activity than those of reference compounds, oleanolic acid (76.5 $\mu\textrm{g}$/$m\ell$ for PPE, 219.2 $\mu\textrm{g}$/$m\ell$ for HNE) and ursolic acid (31.0 $\mu\textrm{g}$/$m\ell$ for PPE, 118.6 $\mu\textrm{g}$/$m\ell$ for HNE). According to the Lineweaver-Burk Plots, the inhibition against both PPE and HNE by this phenolic substance was competitive with substrate. Phenolic substance from Areca catechu exhibited high free radical scavenging effect ($SC_{50}$/ : 6 $\mu\textrm{g}$/$m\ell$) and inhibited effectively hyaluronidase activity ($IC_{50}$/: 210 $\mu\textrm{g}$/$m\ell$). These results suggest that the Phenolic substance Purified from Areca catechu showed anti-aging effect by protecting connective tissue proteins.

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.4
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• pp.189-194
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• 1975

On a newly reclaimed acidic soil, investigation was made to find out the influence of lime and P application at a largeg uantity on the uptake of Zn by corn and its distribution among the leaves, under afactorial combination with two levels of Zn, lime, and P. The results are summarized as following. 1. The application of lime for neutralization of soil significantly reduced the uptake of Zn by corn. 2. Liberal doses of P (5% of P absorption coefficient of soil; 500ppm) applied before sowing did not influence the uptake of Zn. 3. The concentration of Zn in bottom leaves better reflected the Zn uptake status of corn than the upper leaves. The concentration of Zn in bottom leaves responded clearly to the treatments of lime and Zn, while those of upper leaves tended to be constant unresponding to application of lime and Zn. 4. On the present experimental condition, the yield of corn was increased with the increase of P application. But the concentration of P in plant tissue remained constant under different P levels. 5. Application of lime (calcium hydroxide) on low P plots, depressed the yield of corn significantly. It was speculated that the lime applied at large dose made the applied P less available to corn.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.2
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• pp.107-113
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• 1987

A pot experiment was carried out using Seomjinbyeo to investigate the effects of some sulfur-containing substances on the growth of rice plants and on soil characteristics. The results are summarized as follows: 1. Soil pH in plots given the sulfur treatments was lower than that in the urea plot during the growing stages. Soil pH in plots given the mineral sulfur treatments was higher than that in the ammonium sulfate plot at the early growth stage of rice. However, as the stage of rice growth progressed, soil pH became similar. After the experiment, soil pH was higher, but the sulfur content of soil was lower in the urea plot compared with that in other sulfur plots. After the experiment, the sulfur content of soil in the ammonium sulfate plot was the lowest among the plots given the sulfur treatments. 2. Plant heights and the number of tillers of the rice plants in plots given the sulfur treatments were higher than those in the urea plot, specially in the number of tillers of the rice plants. 3. Nitrogen and sulfur contents of the tops of rice plants in plots given the sulfur treatments were slightly higher than those in the urea plot. nitrogen and sulfur contents in the ammonium sulfate plot were higher than those in plots given the mineral sulfur treatments at the early growth stage of rice. However, as the stage of rice growth progressed, those in plots given the mineral sulfur treatments were higher. 4. The chlorophyll content in the leaf blade at heading stage in plots given the gypsume and given the mineral sulfur treatments was significantly higher than that in the urea plot. The N/S ratio was negatively correlated with the chlorophyll content.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.1
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• pp.61-66
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• 1989

Plants were exposed to different sub-optimal growth temperature regimes for hardening or sprayed with abscisic acid (ABA) before being placed at $10^{\circ}C$ for three days. Comparisons were made with non-treated plants for plant survival, photosynthetic activities and fatty acids composition of phospholipids. The level of endogenous ABA of the hardened seedlings was compared with that in control and ABA sprayed-seedlings. The results of the above experiment were summarized as follows: 1. Compared to non-treated plants, the hardening and ABA treatment resulted in higher plant survival rate and better seedling growth following earlier transplanting. The low temperature hardening which gradually increased the duration of the low temperature treatment at 2-day intervals was more effective than the controled temperature hardening which gradually decreased the temperature from $25^{\circ}C$ to $20^{\circ}C$ and $15^{\circ}C$. 2. The photosynthetic activities of rice leaves after 3-day exposure to $10^{\circ}C$ were higher in hardened and ABA sprayed seedlings than in non-treated plants. The root activities after low temperature treatment were also higher in hardened and ABA sprayed seedlings than in control. 3. Fatty acids of phospholipids in rice plants such as stearic acid and oleic acid were decreased and the highly unsaturated fatty acids, linoleic acid and lenolenic acid were increased by hardening or ABA treatments. It is assumed that the increase of cold tolerance in these plants was due to the higher ratio of unsaturated to saturated fatty acids. Respectively, the ratio was 1.86, 1.97 and 1.80 in plants given controled temperature hardening, the low temperature hardening and ABA treatments. While in control plants, it was 1.17. 4. ABA content in rice seedling given the low temperature hardening treatment was 67.2 ng/g F.W which was approximately twice that of non-treated control, which was 33.9 ng/f F. W., in contrast, the ABA content of the ABA treated seedlings was 91.7 ng/g F. W.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.1
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• pp.45-52
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• 1989

The effects of soil properties and N, P and K application rates on the contents of inorganic constituents in the rhizomas of Ji-whang were investigated to know the condition for producing the better quality rhizoma of Ji-whang. Most of soil texture for Ji-whang cultivating belongs to sandy clay loam and sandy loam soils. The nitrogen contents in the rhizomas of Ji-whang was negatively correlated with the contents of organic matter and total nitrogen in surface soils, whereas positively correlated with the sand contents in subsoils. The calcium contents in the rhizomas of Ji-whang were negatively correlated with the sand contents, however, positively correlated with the contents of clay and organic matter in soils. The iron contents was positively correlated with the contents of clay, available phosphorus and exchangeable potassium in surface soils. Much more contents of the inorganic constituents in the leaf of Ji-whang was generally detected than that in the rhizoma of Ji-whang. The contents of iron and copper in the leaf was determined to be more than that in the rhizomas by ten times. In the results of field experiments, it is apparent that the nitrogen contents in the rhizoma and leaf increased with increasing N application rate more than 20kg/10a and 10kg/10a, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.1
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• pp.1-7
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• 2001

Total upland area for cultivating the vegetable crops in the Alpine soils of Northeastern South Korea has been extending its limit to meet the increasing demand of vegetable food in recent decades. About 70% of these alpine soils are located in over 7% of the slope and most of vegetable crops have been cultivated intensively without practicing the best management systems. Thus, soil erosion and continuous cropping system have degenerated the soil fertility and shown detrimental effects on water quality. We initiated an intensive and extensive investigation to characterize the fertility problems encountered in these uplands. Objectives of this paper were to characterize the fertility status in the Alpine soils cultivated with vegetable crops for many years and to provide the recommendations for adequate soil management measures including fertilization and erosion control. Soils in general have good drainage with textural classes of loam or sandy loam. Their topographical characteristics tended to lead them to shallow plow layers, and the steepness of the terrain created erosion hazard. Of the soils examined, about 11% of uplands over 30% gradient was found in need of an urgent reforestation. A high content of gravel and firm hardness of soil attributed to inhibit the utilization of farm machinery and plant-root development. The average soil pH 5.6 was slightly low relative to pH 5.70 of the national average. Organic matter content was high compared with 2.0% of national average, but decreased with the prolonged cultivation periods. Available $P_2O_5$ concentration was unusually high due to the consequence of over dose application with chemical and organic fertilizers. Exchangeable cations as Ca, Mg, and K were appeared to be decreased in these regions with prolonging the cultivation periods. There were no significant differences in cation exchange capacity (CEC) and electrical conductivity (EC) among locations. Heavy metal contents were mostly lower than the threshold of danger level designated by Soil Environment Conservation Law of South Korea. Results indicated that a proper countermeasure and the best management practice should be immediately implemented to conserve the top soil and fertility in the Alpine regions.