• Asian-Australasian Journal of Animal Sciences
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• 제21권4호
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• pp.494-498
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• 2008

MTT (3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) reduction assay is a method that validates the viability of an active cell. Dehydrogenase in mitochondria converts yellow colored insoluble tetrazolium salt to purple colored water-soluble formazan. Sperm also have mitochondria in the midpiece, therefore sperm viability could be evaluated by MTT reduction assay. Several studies have already demonstrated the capability of application of the MTT reduction assay to sperm of several species in Hepes-BSA buffer. Because most liquid semen was diluted in extender like BTS (Beltsville Thawing Solution), Modena or Androhep when it is used or transferred, semen needed another dilution in Hepes-BSA buffer to assess sperm viability. In this study, we evaluated boar sperm viability especially in BTS extended semen and compared the efficiency of this test with eosin-nigrosin staining. We used the fresh BTS extended semen from a local A.I center. Semen sample was diluted to $3.0{\times}10^7$ sperms/ml in BTS. The rates of formazan production were measured in 96-well microtiter plates immediately and 1h after incubation at $17^{\circ}C$ using a spectrophotometer at wave length 560 nm. Simultaneously, split samples of the same semen were tested, using eosin-nigrosin staining to compare the efficiency of the MTT assay of sperm viability in BTS. The correlation between the results of these tests was calculated using Student-t test and ANOVA. The results revealed a strong correlation between the results of MTT reduction rate and the results that were simultaneously determined by eosin-nigrosin staining at 1 h. In conclusion, the MTT reduction test was an effective and simple method to validate sperm viability and it could be used as a simple tool to evaluate sperm viability in the local A.I center and laboratory.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 1999년도 추계학술발표회
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• pp.81-84
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• 1999

The aqueous geochemical characteristics of Cr(III) and Cr(Ⅵ) in environmental systems are very different from one another: Cr(Ⅵ) is highly soluble, mobile and toxic relative to Cr(III) Reduction of Cr(Ⅵ) to Cr(III) are beneficial in aquatic systems because of the transformation of a highly mobile and toxic species to one having a low solubility in water, thus simultaneously decreasing chromium mobility and toxicity. Fe(II) species are excellent reductants for transforming Cr(Ⅵ) to Cr(III), and in addition, keeping Cr(III) concentrations below the drinking water standard of 52 ppb at pH values between 5 and 11. Investigations of the effects of NOM on Cr(Ⅵ) reduction are for examining the feasibility of using ferrous iron to reduce hexavalent chromium in subsurface environments. Experiments in the presence of soils, however, showed that the solid phase consumes some of the reducing capacity of Fe(II) and makes the overall reduction kinetics slower. The soil components bring about consumption of the ferrous iron reductant. Particular attention is devoted to the complexation of Fe(II) by NOM and the subsequent effect on Cr(Ⅵ) reduction. Cr(Ⅵ) reduction rate by Fe(II) was affected by the presence of NOM (humic acid), The effects of humic acid was different from the solution pH values and the concentration of humic acid. It was probably due to the reactions between humic acid and Cr(Ⅵ), humic acid and Fe(II), and between Cr(Ⅵ) and Fe(II), at each pH.

• 태양에너지
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• 제18권4호
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• pp.1-10
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• 1998

Polymer induced turbulent drag reduction in a rotating disk apparatus was investigated using four different molecular weights of poly(ethylene oxide)(PEO) in a synthetic seawater solution for the purpose of potential application to the cold water piping in the Ocean Thermal Energy Conversion(OTEC) system. To apply drag reduction to the OTEC we measured the temperature dependence on the drag reduction efficiency. From this study, it was found that the drag reduction efficiency increases with the temperature and the concentration. To measure the drag reduction efficiency during the operation period, the drag reduction behavior was detected as a function of time and the results obtained from the experiment was compared to the Brostow's model equation.

• Journal of Advanced Marine Engineering and Technology
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• 제39권3호
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• pp.243-247
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• 2015

본 연구는 상류측에 작은 삼각주를 가진 정방형주의 유체력 저감 특성을 양 항력 측정 실험으로 파악한 것이다. 실험파라메터는 정방형주 한 변의 길이에 대한 삼각주의 폭비(H/B=0.2~0.6) 및 정방형주 전면에서부터 삼각주까지의 거리 (G/B=0~3) 로 했다. 삼각주의 폭비를 고정시킨 경우 정방형주의 항력감소율은 간격비가 증가할수록 증가하다 감소하는 특성을 보였다. 또한 같은 간격비에서는 삼각주의 폭비가 클수록 정방형주의 항력감소율이 증가했으며, 폭비 H/B=0.6, 간격비 G/B=1.5일 때 최대 78.5%의 항력감소율을 보였다. 정방형주의 양력감소율은 수직평판의 폭비 및 간격비에 거의 영향을 받지 않았으며, 양력감소율의 평균치는 52.4% 정도였다.

• 청정기술
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• 제20권1호
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• pp.28-34
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• 2014

본 연구에서는 나노 크기의 결정 구조를 가진 타이타니아 담체를 용매열합성법(solvothermal method)을 활용하여 합성한 후 팔라듐과 구리를 담지한 촉매를 제조하였다. 제조된 촉매를 수중 질산성 질소 저감 반응에 적용한 결과, 타이타니아 담체의 결정 크기가 반응 활성에 영향을 미치는 것이 확인되었다. 결정 크기가 작은 담체를 활용한 촉매가 더 빠른 속도로 질산성 질소를 저감하였지만, 반응 중 pH가 높게 형성되어 질소 선택도가 매우 낮은 현상을 보였다. 이를 해결하기 위해 pH 완충제인 이산화탄소를 공급하여 질소 선택도를 약 60% 증가시켰다. 상기에 언급한 촉매를 대상으로 질소 흡-탈착, X-ray diffraction (XRD), $H_2$-temperature programmed reduction (TPR), X-ray photoelectron spectroscopy (XPS) 등의 다양한 특성화 분석을 수행하여 촉매의 반응활성과 물성간의 상관관계에 대해 조사하였다.

• Journal of Electrochemical Science and Technology
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• 제13권4호
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• pp.462-471
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• 2022

In this study, the electrochemical impedance characteristics of CO₂/H₂O co-reduction to produce CO/H₂ syngas were investigated in a low-temperature single cell. The effect of the operating conditions on the single-cell performance was evaluated at different feed concentrations and cell voltages, and the corresponding electrochemical impedance spectroscopy (EIS) data were collected and analyzed. The Nyquist plots exhibited two semicircles with separated characteristic frequencies of approximately 1 kHz and tens of Hz. The high-frequency semicircles, which depend only on the catholyte concentration, could be correlated to the charge transfer processes in competitive CO₂ reduction and hydrogen evolution reactions at the cathodes. The EIS characteristics of the CO₂/H₂O co-reduction single cell could be explained by the equivalent circuit suggested in this study. In this circuit, the cathodic mass transfer and anodic charge transfer processes are collectively represented by a parallel combination of resistance and a constant phase element to show low-frequency semicircles. Through nonlinear fitting using the equivalent circuit, the parameters for each electrochemical element, such as polarization resistances for high- and low-frequency processes, could be quantified as functions of feed concentration and cell voltage.

• 지질공학
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• 제3권2호
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• pp.191-201
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• 1993

환원은 심층 지하처분되는 Cr(VI)의 양을 줄이는데 중요한 수단의 하나이다. 이 반응은 pH와 온도에 따라 민감하게 작용하며, 지하심층 처분장의 낮은 pH와 높은 온도는 토양 유기질, 높은 염소이온 농도와 더불어 Cr(VI)의 환원에 좋은 환경을 제공한다. 본 연구에서는 다양한 환경 조건에 따른 토양 유기질과 염소이온에 의한 Cr(VI)의 환원이 조사 되었다. aquifer와 aquitard 샘플은 각각 미국 루이지애나주 세인트 촬스군의 심층 처분장에서 채취되었다. 본 연구에서는 심층처분장을 대표하는 pH(-0.81~2.0), 온도($50^{\circ}C와{\;}70^{\circ}C$), 염소이온 농도(0, 0.26, 0.52 몰)를 이용하였다.

• Journal of Ecology and Environment
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• 제37권2호
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• pp.91-97
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• 2014

Little is known about how the increased porosity of a deciduous windbreak, which results from loss of leaves, influences wind speed reduction. We hypothesized that, with loss of foliage, the wind speed reduction effectiveness of a deciduous windbreak decreases on near leeward side but not on further leeward side and that wind speed recovers faster in the full foliage season than in other seasons. During summer, autumn, and winter (full, medium, and non-foliage season, respectively), we observed wind speed and direction around a deciduous windbreak in a traditional Korean village on windward and near and further leeward sides (at -8H, 2H, and 6H; H = 20 m, a windbreak height). We used a linear mixed effects model to determine that the relative wind speed reduction at 2H significantly decreased from 83% to 48% ($F_{2,111.97}=73.6$, P < 0.0001) with the loss of foliage. However, the relative wind speed reduction at 6H significantly increased from 26% to 43% ($F_{2,98.54}=18.5$, P < 0.0001). Consequently, wind speed recovery rate between 2H and 6H in summer was two times higher than in autumn and ten times higher than in winter ($F_{2,102.93}=223.1$, P < 0.0001). These results indicate that deciduous windbreaks with full foliage seem to induce large turbulence and increase wind speed recovery rate on leeward side. Our study suggests that further research is needed to find the optimal foliage density of a deciduous windbreak for maximizing windbreak effectiveness regardless of seasonal foliage changes.

• 한국환경농학회지
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• 제24권3호
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• pp.203-209
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• 2005

실험에 사용된 영가 철의 평균 입도는 $85.55{\sim}196.46{\mu}m$의 범위를 나타내었고 비표면적은 $0.055{\sim}0.091m^2/g$으로 나타났다. 영가 철 중 J(93%) 및 PU(88%)를 제외한 PA, F, S, J1는 철을 98% 이상 함유하는 것으로 평가되었다. 영가 철에 의한 Cr(VI)의 환원은 영가 철의 종류에 따라 달랐으며, J 및 PU의 경우 3시간 이내에 98% 이상의 Cr(VI)을 제거하였다. 그러나 PA, F, S, J1의 경우 반응 48시간 동안 각각 74, 65, 29, 11%의 Cr(VI) 만을 제거하였으며 이때 환원 효율은 J > PU > PA > F > S > J1 순으로 나타났다. 영가철에 의한Cr(VI)의 환원 과정에서 용액의 pH는 반응초기 3시간 동안 급격히 증가하였으며 이후 안정화되는 경향을 나타내었다. 영가 철의 종류에 따른 pH의 변화는 PU > J > PA > F > S > J1 순으로 나타났으며 Cr(VI)의 환원효율이 큰 영가 철에서 pH의 상승 폭도 크게 나타나는 것으로 평가되었다. 영가 철의 종류에 따른 산화환원전위 Eh의 변화는 pH와는 반대의 경향을 나타내었으며 Cr(VI)의 환원율이 클수록 Eh의 감소율은 증가하였다. 유기화합물의 경우 반응효율이 영가 철의 물리적 특성에 의해 주로 지배되는 것으로 알려져 있으나 Cr(VI)의 경우 화학적 특성이 반응효율을 지배하는 것으로 판단되었다. 본 실험에 사용된 6종류의 상용 영가 철 중에는 J와 PU가 Cr(VI)의 환원에 가장 효과적인 것으로 조사되었다.

• Environmental Engineering Research
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• 제11권3호
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• pp.149-155
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• 2006

NOx reduction experiments were conducted by direct injection of urea into a diesel fueled, combustion-driven flow reactor which simulated a single engine cylinder ($966cm^3$). NOx reduction tests were carried out over a wide range of air/fuel ratios (A/F=20-40) using an initial NOx level of 530ppm, and for normalized stoichiometric ratios of reductant to NOx (NSR) of 1.5 to 4.0. The results show that effective NOx reduction with urea occurred over an injection temperature range of 1100 to 1350K. NOx reduction increased with increasing NSR values, and about a 40%-60% reduction of NOx was achieved with NSR=1.5-4.0. Most of the NOx reduction occurred within the cylinder and head section (residence time <40msec), since temperatures in the exhaust pipe were too low for additional NOx reduction. Relatively low NOx reduction is believed to be due to the existence of higher levels of CO and unburned hydrocarbons (UHC)inside the cylinder, and large temperature drops along the reactor. Injection of secondary combustible additives (diesel fuel/$C_2H_6$) into the exhaust pipe promoted further substantial NOx reduction (5%-30%) without shifting the temperature windows. Diesel fuel was found to enhance NOx reduction more than $C_2H_6$, and finally practical implications are further discussed.