• Title/Summary/Keyword: +S9 Mix

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Antimutagenic Effects of Juices from Edible Korean Wild Herbs

  • Ham, Seung-Shi;Oh, Deog-Hwan;Hong, Jeong-Kee;Lee, Jae-Hoon
    • Preventive Nutrition and Food Science
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    • v.2 no.2
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    • pp.155-161
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    • 1997
  • The mutagenic and antimutagenic activities of juices from 20 common edible wild herbs found in Korea were investigated using the spore-rec assay and Ames test. The juices of Hemerocallis fulva and Capsella bursapastoris exhibited a little induction or inhibition of mutagenesis in the presence of selected metal ions, but juices of most edible wild herbs did not affect on the mutagenesis in the spore-rec assay. In the other hand, all of the juices strongly inhibited the mutagenesis induced by benzo[a]pyrene, 2-amino-fluorene, and 3-amino-1,4-dimethyl-5H-pyridol tested on Salmonella typhimurium TA98 or TA100 in the presence of S-9 mix. The antimutagenic effects increased as the concentration of the mutagens increase. The results suggest that concentration of samples or types of various mutagen interact to affect the antimutagenic potential of the juices in the TA98 and TA100 strain.

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Genotoxic evaluation of Gyllus bimaculatus in 3 sets of mutagenicity tests

  • Ahn, Mi-Young;Pae, Hey-Jin;Lee, Byung-Mu;Ryu, Kang-Sun
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.120.1-120.1
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    • 2003
  • The mutagenic potential of Gryllus bimaculatus was evaluated using the short-term genotoxicity tests including Ames, chromosome aberration and micronuclei tests. In salmonella typhimurium assay, G. bimaculatus did not show any mutagenic response in the absence or presence of S9 mix with TA98, TAl00, TA1535, and TA1537. In chromosome aberration test, G. bimaculatus did not show any significant effect on Chinese Hamster Ovary (CHO) cells compared with control. (omitted)

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Volatile Aroma Compounds in Ice Cream Prepared from Lactic Fermented Egg White Food Added with Cream (크림첨가 난백젖산균발효식품으로 만든 아이스크림의 휘발성향기성분)

  • Ko, Young-Tae;Kim, Tae-Eun;Kang, Jung-Hwa
    • Korean Journal of Food Science and Technology
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    • v.33 no.3
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    • pp.373-377
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    • 2001
  • Egg white mix (EWM) added with different ratios of cream $(10{\sim}15%,v/v)$ was fermented with Lactobacillus acidophilus (KCTC 2182) and changes of volatile aroma compounds during fermentation for 21 h were investigated. The amount of volatile aroma compounds between pre-fermented EWM, 15 h-fermented EWM, soft ice cream prepared from fermented EWM, and hard ice cream prepared from soft ice cream was compared. The results were as follows: (1) The amount of ethanol increased gradually until 9 h and increased rapidly to maximum value at 12 h and then decreased rapidly. The amount of acetone at 12 h was slightly higher, but it was not changed markedly during fermentation. Diacetyl was detected at 3 h and increased gradually to maximum value until 15 h and then decreased slightly. The amount of butanol was not changed markedly during fermentation. Acetoin was detected at 3 h and increased rapidly until 15 h and then increased gradually. (2) The amount of acetone, ethanol, diacetyl and butanol of pre-fermented sample, 15 h-fermented sample, soft ice cream and hard ice cream increased in proportion to the amount of cream added to EWM. However, the amount of butanol was relatively constant regardless of added cream ratio. The amount of volatile aroma compounds of soft ice cream was higher than that of other samlples, while that of pre-fermented sample was lower than that of other samples. Diacetyl and acetoin were not detected in pre-fermented sample.

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Studies on Antimutagenic Effects and Gene Repair of Enzymatic Browning Reaction Products (효소적 갈변반응 생성물의 돌연변이 억제효과 및 유전자 수복에 관한 연구)

  • Ham, Seung-Shi;Kim, Sung-Wan;Kim, Young-Myung
    • Korean Journal of Food Science and Technology
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    • v.22 no.6
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    • pp.632-639
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    • 1990
  • The biological activities of twelve different kinds of enzymatic browning reaction products(EBRP), which resulted from the reactants four kinds of polyphenols with polyphenol oxidase extracted from Ligularia fischeri, pimpinella brachycarpa and Aster scaber of edible mountain herbs. All of twelve samples did not show any mutagenic effect in the spore rec-assay, Ames mutagenicity test and DNA breaking test. However metal ions such as $Cu^{2+},\;Fe^{2+}$, and $Ni^{2+}$ were increased the DNA breakage in rec-assay. The EBRPs inhibited the mutagenicities induced by $benzo({\alpha})pyrene (B({\alpha})P)$, 3-amino-1,4-dimethyl-5H-pyrido-[4,3-b]indole(Trp-P-1) and 2-aminofluorene(2-AF) in Salmonella/microsome assay system with S-9 mix. In effects of EBRPs on the DNA repair system, the activity of EcoRI was highly inhibited and that of $T_{4}$ DNA ligase was inactivated by addition of EBRPs. The results of transformation ratio of plasmid pGA658 into E. coli HB 101 was significantly decreased by the reaction products of S. brachycarpa polyphenoloxidase (PPO). When UV light was exposed to the mixture of DNA and EBRP before the thanformation, the reaction products from L. fischeri PPO with pyrogallol, catechol and hydroxyhydroquinone stimulated transformation ratio.

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Analysis of Marketing Strategy in Domestic Online Luxury Fashion Platform (국내 온라인 명품 패션 플랫폼 마케팅전략 분석)

  • Min Gyung Lee;Hyeon Ju Kim
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.1
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    • pp.361-372
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    • 2023
  • In this study, three luxury fashion start-up platforms, Balaan, Trenbe, and Must-it, were selected as research subjects. The purpose of this study is to compare and analyze the marketing mix strategies of each of the three online sites. The results of our study are as follows. First of all, the product strategies of the three luxury platform companies are characterized by the composition of products from high-end brands to SPA brands, and product composition such as kids, home living, Used goods and art in addition to women's and men's wear. In addition, the pricing strategies of luxury platforms show price differences depending on the luxury platform even for the same product. It is shown as a structure that directly determines margin. Therefore, in order to secure an edge in price competitiveness, each platform provided discount coupons and savings that are not available in offline stores such as department stores, providing opportunities to purchase luxury goods at a lower price than offline stores.Lastly, the sales promotion strategies of the three luxury platform companies was used include price discount promotions such as price discounts, discount coupons, and regular sales, and value-added sales such as membership registration/review points, events, product information, delivery services, social contribution activities, and SNS utilization.

Prediction of residual compressive strength of fly ash based concrete exposed to high temperature using GEP

  • Tran M. Tung;Duc-Hien Le;Olusola E. Babalola
    • Computers and Concrete
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    • v.31 no.2
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    • pp.111-121
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    • 2023
  • The influence of material composition such as aggregate types, addition of supplementary cementitious materials as well as exposed temperature levels have significant impacts on concrete residual mechanical strength properties when exposed to elevated temperature. This study is based on data obtained from literature for fly ash blended concrete produced with natural and recycled concrete aggregates to efficiently develop prediction models for estimating its residual compressive strength after exposure to high temperatures. To achieve this, an extensive database that contains different mix proportions of fly ash blended concrete was gathered from published articles. The specific design variables considered were percentage replacement level of Recycled Concrete Aggregate (RCA) in the mix, fly ash content (FA), Water to Binder Ratio (W/B), and exposed Temperature level. Thereafter, a simplified mathematical equation for the prediction of concrete's residual compressive strength using Gene Expression Programming (GEP) was developed. The relative importance of each variable on the model outputs was also determined through global sensitivity analysis. The GEP model performance was validated using different statistical fitness formulas including R2, MSE, RMSE, RAE, and MAE in which high R2 values above 0.9 are obtained in both the training and validation phase. The low measured errors (e.g., mean square error and mean absolute error are in the range of 0.0160 - 0.0327 and 0.0912 - 0.1281 MPa, respectively) in the developed model also indicate high efficiency and accuracy of the model in predicting the residual compressive strength of fly ash blended concrete exposed to elevated temperatures.

Mechanical Properties and Modeling of Amorphous Metallic Fiber-Reinforced Concrete in Compression

  • Dinh, Ngoc-Hieu;Choi, Kyoung-Kyu;Kim, Hee-Seung
    • International Journal of Concrete Structures and Materials
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    • v.10 no.2
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    • pp.221-236
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    • 2016
  • The aim of this paper is to investigate the compressive behavior and characteristics of amorphous metallic fiber-reinforced concrete (AMFRC). Compressive tests were carried out for two primary parameters: fiber volume fractions ($V_f$) of 0, 0.3, 0.6 and 0.8 %; and design compressive strengths of 27, 35, and 50 MPa at the age of 28 days. Test results indicated that the addition of amorphous metallic fibers in concrete mixture enhances the toughness, strain corresponding to peak stress, and Poisson's ratio at high stress level, while the compressive strength at the 28-th day is less affected and the modulus of elasticity is reduced. Based on the experimental results, prediction equations were proposed for the modulus of elasticity and strain at peak stress as functions of fiber volume fraction and concrete compressive strength. In addition, an analytical model representing the entire stress-strain relationship of AMFRC in compression was proposed and validated with test results for each concrete mix. The comparison showed that the proposed modeling approach can properly simulate the entire stress-strain relationship of AMFRC as well as the primary mechanical properties in compression including the modulus of elasticity and strain at peak stress.

The Antimutagenic and Antioxidant Effects of Red Pepper Seed and Red Pepper Pericarp (Capsicum annuum L.)

  • Sim, Ki-Hyeon;Han, Young-Sil
    • Preventive Nutrition and Food Science
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    • v.12 no.4
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    • pp.273-278
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    • 2007
  • In this study, we examined the antimutagenicity of red pepper seed and red pepper pericarp ethanol extracts using the standard Ames test in the presence and absence of S9 mix. The extracts showed inhibitory effects on both the TA98 and TA100 Salmenella Typhimurium strains against the mutagenic activity of promutagen 2-aminoanthracene, and were also protective against the directly acting mutagens sodium azide and 2-nitrofluorene. The red pepper seed elicited stronger antimutagenicity than the red pepper pericarp. Both the red pepper seed and red pepper pericarp directly quenched nitric oxide to different degrees and the scavenging activities increased with increasing concentrations. Nitric oxide scavenging activity ranged from $22{\sim}77%$ in the red pepper seed, and from $36{\sim}49%$ in the red pepper pericarp. The TEAC values for red pepper seed extract were $47.89{\pm}1.64mg\;g^{-1}$ in the ABTS radical scavenging assays, while those of red pepper pericarp extract were $94.18{\pm}1.61mg\;g^{-1}$. Therefore, we conclude that red pepper seed and red pepper pericarp have antimutagenic activities as well as antioxidant activity.

Inhibitory Effects of Red Ginseng on Skin Tumor Formation Induced by Ethyl Carbamate Metabolites (에틸카바메이트 대사산물에 의해 유발된 마우스 피부 종양에 대한 홍삼의 억제효과)

  • 박광균;오상환;정원윤
    • Toxicological Research
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    • v.16 no.1
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    • pp.9-16
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    • 2000
  • Ginseng (the root of Panax ginseng C. A. Meyer, Araliaceae) has been used for traditional medicine in China, Korea, Japan and other Asian countries. It is most often used as a general tonic, and it involves a wide range of pharmacological actions, such as antiaging, adaptogen-like effect to foreign deleterious infringement, immunoenhancement, antistress, antitumor, and antioxidant actions. Red ginseng showed anticarcinogenic activity against various chemical carcinogens in mouse and cancer-preventive effect of human being as on mice in experimental and epidemiological studies. In the present study, we have found the protective properties of red ginseng against vinyl carbamate (VC) which is the proximate carcinogen of ethyl carbamate and its ultimate carcinogenic epoxides. Red ginseng exhibited dose-dependent inhibition on the mutagenci activities of boty VC in the presence of S9 mix and vinyl carbamate epoxide (VCO) without metabolic activation in Salmonella typhimurium TA1535. Formation of DNA adducts from VCO was also attenuated in the presence of red ginseng. Oral administration of red ginseng prior to the topical application of each of the above carcinogens and TPA treatment resulted in significant reduction in both incidence and multiplicity of skin tumors in mice. These results indicate that red ginseng possesses a strong chemopreventive effect against mouse skin carcinogenesis induced by VC or VCO.

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Spatial Regeneration for Preservation of Historic Urban Features and Improvement of Living Conditions in High-density Historic Districts: Urban Renewal Design Strategies for Shanghai's Old Town

  • Huang, Na;Cai, Yongjie;Zhuang, Mingyu;Zhou, Yi;Zhou, Jun
    • International Journal of High-Rise Buildings
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    • v.10 no.1
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    • pp.9-16
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    • 2021
  • Being the origin of Shanghai City, the Old Town presents a high-density spatial texture, a characteristic of local living conditions in the Shanghai context. However, the Old Town is faced with competing interests: the preservation of historic urban features and the improvement of contemporary living conditions. In view of its high density and poor living conditions, this paper focuses on two types of blocks for urban design research, and proposes two spatial regeneration strategies, as "overlapping lilong" and "texture continuity". It is expected to inherit the regional characteristics of urban space, improve the plot ratio and supplement the mix of functions, through the translation of the traditional lilong typology and the reproduction of historical streets and alleys, so as to provide operable spatial strategies and design methods for the organic renewal of Old Town and other historic districts.