• Biotechnology and Bioprocess Engineering:BBE
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• 제9권6호
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• pp.523-527
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• 2004

Recombinant Saccharomyces cerevisiae expression systems were developed to pro­duce a novel human anti-angiogenic protein called LK8, an 86 amino-acid kringle fragment pro­tein with three disulfide linkages. Galactose-inducible LK8 expression plasmid was constructed, and LK8 production levels by four S. cerevisiae strains were compared in order to select an op­timal host strain. S. cerevisiae 2805 was the most efficient among the strains tested. Elevating the LK8 gene copy number through multiple integration using 8-sequences as target sites re­sulted in more than a two-fold increase in the LK8 production level compared with the plasmid­based expression system. The maximum LK8 protein concentration of 25 mg/L was obtained from batch cultivation of the yeast transformant that harbors 16 copies of the LK8 gene. In con­clusion, the strain integrated with the multiple LK8 gene secreted the protein with relatively high yield, although, the increased LK8 gene dosage over 11 copies did not lead to further en­hancement in batch cultivations.

• 제어로봇시스템학회논문지
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• 제21권12호
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• pp.1109-1114
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• 2015

This paper presents an improved learning-based visual inspection method for auto parts inspection in severe lighting changes. Automobile sunroof frames are produced automatically by robots in most production lines. In the sunroof frame manufacturing process, there is a quality problem with some parts such as volts are missed. Instead of manual sampling inspection using some mechanical jig instruments, a learning-based machine vision system was proposed in the previous research[1]. But, in applying the actual sunroof frame production process, the inspection accuracy of the proposed vision system is much lowered because of severe illumination changes. In order to overcome this capricious environment, some selective feature vectors and cascade classifiers are used for each auto parts. And we are able to improve the inspection accuracy through the re-learning concept for the misclassified data. The effectiveness of the proposed visual inspection method is verified through sufficient experiments in a real sunroof production line.

• Journal of Ginseng Research
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• 제40권2호
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• pp.121-126
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• 2016

Background: Ginsenoside F1, a pharmaceutical component of ginseng, is known to have antiaging, antioxidant, anticancer, and keratinocyte protective effects. However, the usage of ginsenoside F1 is restricted owing to the small amount found in Korean ginseng. Methods: To enhance the production of ginsenoside F1 as a 10 g unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the commercial enzyme Cellulase KN from Aspergillus niger with food grade, which has ginsenoside-transforming ability. The proposed optimum reaction conditions of Cellulase KN were pH 5.0 and $50^{\circ}C$. Results: Cellulase KN could effectively transform the ginsenosides Re and Rg1 into F1. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 5.0 and $50^{\circ}C$ for 48 h with protopanaxatriol-type ginsenoside mixture (at a concentration of 10 mg/mL) from ginseng roots. Finally, 13.0 g of F1 was produced from 50 g of protopanaxatriol-type ginsenoside mixture with $91.5{\pm}1.1%$ chromatographic purity. Conclusion: The results suggest that this enzymatic method could be exploited usefully for the preparation of ginsenoside F1 to be used in cosmetic, functional food, and pharmaceutical industries.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.206-215
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• 2005

Polyhydroxyalkanoates (PHAs) are homo or hetero polyesters of (R)-hydroxyalkanoates accumulated in various microorganisms under growth-limiting condition in the presence of excess carbon source. They have been suggested as biodegradable substitutes for chemically synthesized polymers. Recombinant Escherichia coli is one of the promising host strains for the economical production of PHAs, and has been extensively investigated for the process development. The heterologous PHA biosynthetic pathways have been established through the metabolic engineering and inherent metabolic pathways of E. coli have been redirected to supply PHA precursors. Fermentation strategies for cultivating these recombinant E. coli strains have also been developed for the efficient production of PHAs. Nowadays, short-chain-length (SCL) PHAs are being re-invited due to its improved mechanical properties and possible applications in the biomedical area. In this article, recent advances in the development of metabolically engineered E. coli strains for the enhanced production of SCL-PHAs are reviewed. Also, medical applications of SCL-PHAs are discussed.

• 한국멀티미디어학회논문지
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• 제23권4호
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• pp.615-624
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• 2020

By enabling musicians to freely control the elements involved in sound production and tone generation with a variety of timbre, synthesizers have revolutionized and permanently changed music since the 1960s. Paik-Abe Video Synthesizer, a masterpiece of video art maestro Nam June Paik, is a prominent example of re-interpretation of this new musical instrument in the realm of video and audio. This article examines Paik-Abe Video Synthesizer as an innovative instrument to play videos from the perspective of audiovisual art, and establishes its aesthetic value and significance through both artistic and technical analysis. The instrument, which embodied the concept of image sampling and real-time interactive video as an image-based multi-channel music production tool, contributed to establishing a new relationship between sound and image within the realm of audiovisual art. The fact that his video synthesizer not only adds image to sound, but also presents a complete fusion of image and sound as an image instrument with musical characteristics, becomes highly meaningful in this age of synesthesia.

• 복식문화연구
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• 제27권1호
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• pp.57-63
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• 2019

Due principally to the desire to seek lower production costs, the bulk of the world's textile and clothing manufacture migrated to low-cost zones, mainly outside Europe, over the course of the late-twentieth century. In the early-twenty-first century, fast fashion became a dominant force worldwide, with 'Western' retail buyers hunting cheaper deals from clothing manufacturers (mainly in Asia), and with occasional disasters not changing matters beyond the duration of a fashion season. Progressively, seams became narrower, cheaper raw materials were used and durability was no longer an aim. Why bother to do otherwise? This was what the 'Western' consumer wanted: fashion to be worn only a few times and then discarded, despite the fact that vast amounts of human, technological and financial resources were wasted in such a quest. By the end of the second decade of the twenty-first century, the production of textile and clothing products continued to contribute substantially to global warming. This paper reviews briefly the current conditions of manufacture, and argues that the research agenda should be focused on addressing the implications of a progressively changed focus, not on fast-fashion products, but instead on the production of products with greater durability. Meanwhile 'Western' consumers need to turn away from fast fashion and realise that waste is bad for their economy and their society. It is argued further, that after a period of re-adjustment, substantial financial rewards await the national textile and clothing industries that undergo such a turn around.

• 한국식품영양학회지
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• 제34권1호
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• pp.69-78
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• 2021

The aim of this study was to evaluate the basic data of Cirsium setidens Nakai fine powder (FPC), which will then be used in the development of functional fooditems. We measures and evaluated the level of pectolinarin content, phenol content, flavonoids content, antioxidants and anti-obesity properties of FPC. Our results from the study showed that the pectolinarin, phenol, and flavonoids contents of FPC measured at 10.95±0.1 5mg/g, 12.92±0.18 mg gallic acid equivalent (GAE)/g and 26.47±0.77 mg rutin equivalent (RE)/g, respectively. The exhibited antioxidant activity of FPC increased significantly depending on the dosage, and additionally. FPC did not show any cytotoxicity up to the dosage level of 500 μg/mL. During adipocyte differentiation, FPC significantly inhibited ROS production and lipid accumulation, compared with the control substance. These results suggest that FPC could be considered a promising resource of natural antioxidants and could serve a variety of health-improving roles in the production of functional food ingredients.

• Journal of Multimedia Information System
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• 제9권1호
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• pp.1-10
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• 2022

China is a big country in animal fur industry. The total production and consumption of fur are increasing year by year. However, the recognition of fur in the fur production process still mainly relies on the visual identification of skilled workers, and the stability and consistency of products cannot be guaranteed. In response to this problem, this paper proposes a feature fusion-based animal fur recognition network on the basis of typical convolutional neural network structure, relying on rapidly developing deep learning techniques. This network superimposes texture feature - the most prominent feature of fur image - into the channel dimension of input image. The output feature map of the first layer convolution is inverted to obtain the inverted feature map and concat it into the original output feature map, then Leaky ReLU is used for activation, which makes full use of the texture information of fur image and the inverted feature information. Experimental results show that the algorithm improves the recognition accuracy by 9.08% on Fur_Recognition dataset and 6.41% on CIFAR-10 dataset. The algorithm in this paper can change the current situation that fur recognition relies on manual visual method to classify, and can lay foundation for improving the efficiency of fur production technology.

• 토지주택연구
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• 제13권2호
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• pp.17-30
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• 2022

본 연구는 문재인 정부에서 공공기관 정책으로 급격하게 추진되었던 '사회적 가치'가 당초 출발점이 되었던 '큰 사회' 어젠다에 비추어볼 때 지향성에 문제가 있었다는 점을 지적하고 새로운 가치지향을 탐색하는 것을 목적으로 한다. 이를 위해 영국의 '큰 사회 논의'에 대해 살펴본 후 '공공가치'와 '공동생산'이라는 대안적 가치와 수단을 제시하였고 이를 실현하기 위한 정책적 시사점을 제안하였다. 이에 따르면 공공기관이 주도했던 기존의 사회적 가치 관련 정책과 방법은 대폭 수정될 필요가 있을 것으로 판단된다.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.737-745
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• 1996

In recent years, with the increase in the consumption of natural resources and energy, global environmental problems have appeared. This is a very serious environmental load on worldwide food production. For this reason, innovative techniques for production of low entropy by using effectively the energy for the ecosystemic agriculture have been expected. In this study, granular ceramics of 2 to 3mm in diameter having electrical charges at the surface were produced, using the natural raw materials of silicate minerals haing excellent moldabilities and sintering properties . Production of water having functions was attempted by effective use of the electrochemical energy of the ceramics with an efficient water treatment apparatus in which the ceramics were fluidized in water, differently from conventional systems. In the experimental results, the EC of water treated with the ceramics was not changed, but the ORP and also the pH and the DO were changed. The speed of oxidation -re uction reaction was high, and the ceramics -treated water enhanced the vigor of seeds. It can be expected that this treatment system, by which the ORP of water can be moderately controlled, is advantageous in controlling the growth of plants.