• 한국식품조리과학회지
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• 제23권1호통권97호
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• pp.19-24
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• 2007

This study investigated the antioxidant and synergistic effects and nitrite scavenging ability of some phenolic compounds(catechin, rutin, quercetin and naringin), The electron donating abilities of naringin, quercetin, rutin and catechin were 6.7%, 92.8%, 87.6% and 92.21%, respectively, The antioxidant activities in O/W emulsion substrates were in order of rutin > quercetin > catechin > naringin. The antioxidant effect of rutin was stranger than that of BHT or ${\alpha}$-tocopherol. ${\alpha}$-tocopherol showed synergistic effect with catechin and quercetin, but ascorbic acid not showed effect. The nitrite scavenging abilities of catechin, quercetin, rutin and naringin were 99.9%, 98.6%, 25.5% and 0.2%, respectively. The nitrite scavenging abilities of quercetin and actechin were very potent as compared with those of BHT and ascorbic acid.

• 약학회지
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• 제45권6호
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• pp.599-603
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• 2001

The node of lotus rhizome (Nelumbo nucifera, Nymphaeaceae) have been used as a traditional medicine for the remedy of hemorrhage, blood stagnancy and thirstiness. To investigate phenolic compound from the node of Nelumbo nucifera, phytochemical isolation and structure elucidation were conducted. Four phenolic compounds were isolated from aqueous methanolic extract and the structure of these compounds were identised as (+)-catechin (1), (+)-gallocatechin (2), (+)-gallocatechin (4u-8)-catechin (3) and scolpoletin (4) respectively by the analysis of spectroscopic evidences and comparisions with the data of authentic samples.

• 약학회지
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• 제53권6호
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• pp.342-350
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• 2009

The interaction of cadmium (II) ion with quercetin and (+)-catechin was investigated in aqueous solution at various pH. The flavonoid/cadmium stochiometries for cadmium (II) binding to quercetin and (+)-catechin have been determined by UV-vis spectroscopy. 1 : 1 Cd (II)-Flavonoid complex had a maximum absorbance and showed the bathochromic shift of the long-wavelength band of the UV-vis spectra in the alkaline pH, that occurs upon complexation, due to a ligandto-metal charge transfer. These results suggest that Cd (II)-flavonoid complex has the optimal condition of chelation in 0.2 M $NH_3$ - 0.2 M $NH_4Cl$ (pH 8.0) solution.

• Applied Biological Chemistry
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• 제37권6호
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• pp.498-502
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• 1994

Cacao bean husk로부터 2종류의 2당체 flavan-3-ol을 분리하였고 $anisaldehyde-H_2SO_4$ 용액, $FeCl_3$ 및 TLC상에서 축합형 탄닌으로 확인되었다. 이들 2종류의 flavan-3-ol 화합물은 procyanidin B-1[epicatechin-$(4{\beta}{\rightarrow}8)$-catechin], procyanidin B-3[catechin-$(4{\alpha}{\rightarrow}8)$-catechin]로 기기분석에 의해 분자구조가 결정되었다. Procyanidin B-1은 0.3 mM에서 50% glucosyltransferase 저해효과가 관찰되었고 비경쟁적 저해작용기작을 보였으며 epicatechin을 함유한 화합물이 저해 효과가 높은 것으로 확인되었다.

• 한국식품과학회지
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• 제31권1호
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• pp.20-23
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• 1999

채취시기 및 엽위별 차카데킨의 함량 및 조성에 대한 정보를 얻고자 다엽을 5월, 7월, 8월에 채취하고 다엽을 1엽 심, 2엽, 3엽, 4엽으로 나누어서 카테킨 함량 및 개별 카테킨 조성을 분석한 결과, 채취시기별로는 8월에 채취한 차엽의 카테킨 함량이 5월이나 7월에 채취한 것보다 더 많았으며, 엽위별로는 1엽 심, 2엽, 3엽, 4엽 중에서 1엽 심에 카테킨의 함량이 가장 많고 4엽이 가장 적었다. 또한 구성 차카테킨의 함량은 EGCG가 가장 많은 양이 함유되어 있었으며, ECG, EC, EGC의 순이었다.

• 한국식품영양과학회지
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• 제34권10호
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• pp.1498-1502
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• 2005

본 연구는 캠벨종 포도 종자 ethyl acetate 분획물로 부터 생리활성 물질을 분리$\cdot$동정하고 분리물질의 생리활성을 검토하기 위하여 수행되었다. 캠벨종 종자 ethyl acetate분획물로부터 sephadex LP-20 column chromatography를 이용하여 활성물질을 단리하고 ${1}^H$ 및 ${13}^C$) NMR 분석을 통하여 (+)-catechin과 (-)-epicatechin을 동정하였다. HPLC를 이용하여 정량 분석한 결과, (+)-catechin은 45.7$\%$의, (-)-epicatechin은 $35.1\%$의 함량을 나타내었으며 이들 화합물이 포도종자 중의 주요한 생리 활성물질로 사료된다. 이들 화합물의 단독 또는 병용 처리 시 항산화 활성, 지질과산화 억제효과 및 자외선 차단 효과를 살펴본 결과, DPPH free radical 소거 활성에서는 (+)-catechin과 (-)-epicatechin 모두가 $RC_{50}$= 11.1 ${\mu}g/mL$와 $RC_{50}$= 10.4 ${\mu}g/mL$으로 높은 free radical 소거 활성을 나타내었으나, 지질 과산화 억제는 (+)-catechin이 나 (-)-epicatechin 단독처리보다는 ethyl acetate 분획물에서 훨씬 효과가 좋은 것으로 나타났다.

• 한국자원식물학회지
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• 제20권1호
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• pp.38-42
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• 2007

The present study was conducted to investigate the translocation of polyphenols, especially catechin derivatives, from mushroom medium mixed with green tea residues into fruiting body of Pleurotus eryngii. Pleurotus eryngii was grown on the media incorporated by mixing or surface-treated with dry materials including leaf petioles and young stems or leaves of green tea. The dry materials treated in medium did not affect plant height and fresh weight of Pleurotus eryngii body. From the samples of Pleurotus eryngii, the eight main catechin derivatives (-)-gallocatechin(GC), (+)-catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epigallocatechin gallate (EGCG), (-)-gallocatechin gallate (GCG), (-)-epicatechin gallate (ECG), and (-)-catechin gallate (EGCG), and caffeine were analyzed quantitatively by HPLC. The results showed that EGC in Pleurotus eryngii was 45% more detected, when incorporated with the dry materials, than untreated control. Especially, content of EGCG was increased in surface-treated Pleurotus eryngii up to 3.2 ppm, while it was not detected or reduced in control and other treatments. Caffeine content was greatly increased regardless of treatment method, compared with control (0.1ppm), showing 44 fold-amount in Pleurotus eryngii at early growth stage when incorporated with the dry materials into medium. The results indicates that functional catechin derivatives of green tea would be partly translocated into Pleurotus eryngii throught incorporation and surface treatment with residues of green tea plants.

• Journal of Nutrition and Health
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• 제34권7호
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• pp.734-740
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• 2001

The purpose of this study was to investigate the effect of green tea catechins on the antioxidative defense enzyme activity of kidney in diabetic rats. Sprague-Dawley male rats weighting 100$\pm$10g were randomly assigned to one normal and three STZ-induced diabetic groups; catechin free diet(DM-0C group), 0.25% catechin diet(DM-0.25C group) and 0.5% catechin diet(DM-0.5C group). Diabetes was induced by intravenous of 55mg/Kg body weight of STZ in sodium citrate buffer(pH 4.3) after 4 weeks feeding of experimental diets. Rats were sacrified at the 6th day of diabetic states. Superoxide dismutase(SOD) activity in kidney was decreased by 25% and 20% in DM-0C and DM-0.25C groups compared with normal group, DM-0.5C group was not significantly different when compared with normal group. Glutathione peroxidase(GSHpx) activity in kidney was were no significant differences the diabetic groups compared to normal group. Xanthin oxidase(XOD) activity was increased by 110% and 63% in DM-0C and DM-0.25C groups compared with normal group, DM-0.5C group was not significantly different when compared with normal group. The contents of superoxide radical(O$_2$)in kindney were 116% and 33%, respectively, higher in DM-0C and DM-0.25C groups than normal group. DM-0.5C group and normal groups were similar levels in their superoxide radical contents of kidneys. Levels of TBARS(thiobarbituric acid reactive substance) in kidney were increased by 62% in DM-0C group, when compared with normal group, but those of DM-0.5C group were similar to that of normal groups. These results indicate that free radical generation system was weakened and free radical scavenger system was enhance in kidney of STZ-induced diabetics rats by dietary catechin. Thereby it may reduce renal disorders such as oxidative damage and aging of tissue.

• 한국식품영양학회지
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• 제31권3호
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• pp.416-424
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• 2018

The physiologically advantageous aspects of green tea have been identified recently and green tea has been a favorite drink of many people. Due to the increased awareness of green tea's positive effects on human health, the demand for foods containing green tea has increased. This has led to the development of diverse green tea-related beverages; thereby many companies in Korea have put a wide variety of manufactured green tea beverages on the market. However, the components within green tea beverages have not been examined in Korea yet. In this study, we investigated the contents of the physiologically functional materials found in green tea, such as catechin, catechin gallate, epicatechin, epicatechin gallate, epigallocatecin gallate, gallocatechin gallate and caffeine. Fifty-six green tea products purchased from the local grocery stores and cafes were analyzed using high performance liquid chromatography (HPLC) analysis. As a result, all tested products contained catechin and caffeine, although the amount of each component was largely different. The total amount of catechin derivatives in the manufactured green tea beverages purchased from cafes was 263.17 mg/L, while they were 61.99 mg/L in the beverages purchased from the local grocery stores. And, to the almost samples the amount of caffeine was proportional to the amount of catechin.

• Journal of the Korean Wood Science and Technology
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• 제51권3호
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• pp.183-196
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• 2023

Recently, the morphological and molecular features of five stain fungi infecting rubberwood (Hevea brasiliensis), namely Paecilomyces maximus, Paecilomyces formosus, Penicillium crustosum, Paecilomyces lecythidis and Aspergillus chevalieri, have been studied. Prior to this study, the authors revealed that catechin from gambir (Uncaria gambir) could inhibit the growth of the white-rot fungus Schizophyllum commune, and it was important to determine the bioactivity of the aforementioned agent against A. chevalieri. The efficacy of the biocidal agent was examined using a laboratory wooden block test. Rubberwood blocks, 8 mm in thickness, 20 mm in width, and 30 mm in length, were impregnated with catechin solution at concentrations of 6%, 9%, 12%, and 15% (w/v) using the vacuum-pressure method, and their bioactivity was monitored over three weeks through visual and scanning electron microscope assessment of fungal growth as well as the discoloration intensity of the wood samples. The results showed that catechin treatment increased the resistance of wood samples to A. chevalieri. Overall, the higher the catechin concentration, the lower the fungal growth. The lowest fungal growth was observed in the wood samples treated with 12% and 15% catechin (score of 0), demonstrating no discoloration. In contrast, the fungal growth score of the untreated wood samples reached 4, indicating severe discoloration. Catechins appear to be adequate biofungicides against stain fungi in rubberwood.