• 한국식품저장유통학회지
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• 제12권3호
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• pp.299-304
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• 2005

감 껍질 추출물을 Sephadex LH-20 및 MCI-gel CHP 20 column을 이용하여 2개의 주요 탄닌을 분리할 수 있었으며, NMR, IR, FAB-mass 등을 이용한 구조 동정 결과(+)-catechin, (+)-gallocatechin으로 확인되었다. 소석회와 보조응집제로 정제 탄닌을 처리할 때 얻어진 탁도, T-N, T-P 및 CODcr 제거율은 gallocatechin이 catechin보다 우수한 것으로 나타났으며, 이는 탄닌 분자의 -OH기가 타 물질과의 반응성이 높아 하수속의 물질과 결합을 하게 되는데 분자구조에 이러한 -OH기가 많을수록 응집 침전하는 능력이 더 우수한 것으로 판단되었다. 또한 단일 탄닌을 첨가하는 것보다 혼합 탄닌 실험군이 탁도, T-N, T-P 및 CODcr 제거율이 다소 높은 것으로 나타나, 물질 간 synergy 효과도 있는 것으로 확인되었다.

• 생약학회지
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• 제10권2호
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• pp.41-53
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• 1979

A 6', 8a-bonded catechin dimer was synthesized by action of phenoloxidase on 2mol (+)-catechins. The same dimer and other two dimers which are also 6', 8a-bonded were isolated from the fresh cortex of Quercus robur. The 4, 8a-bonded and the etherified 4, 8a-bonded dimers were isolated from it. It was discussed about the meaning of the enzymatically produced 6', 8a-bonded dimers in plant cell. The isolated dimers are as following: 1. 6', 8a-bonded dimers: (+)-catechin-(+)-catechin-6', 8a-dimer(IX), (+)-catechin-(+)-gallocatechin-6', 8a-dimar (XIII), (+)-gallocatechin-(+)-catechin-6', 8a-dimer(XIV). 2. 4, 8a-bonded dimers: (+)-catechin-(+)-catechin-4, 8a-dimer(X), (-)-epicatechin-(+)-catechin-4, 8a-dimer (XI), (+) -galloatechin-(+)-catechin-4, 8a-dimer (XII). 3. 4,8a-bonded dimers with ether linkage: etherified(+)-catechin-(+)-catechin-4, 8a-dimer (XV), etherifid (+)-catechin-(+)-gallocatechin-4, 8a-dimer (XVI), etherified (+)-gallocatechin-(+)-catechin-4, 6a-dimer (XVII).

• 약학회지
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• 제45권6호
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• pp.599-603
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• 2001

The node of lotus rhizome (Nelumbo nucifera, Nymphaeaceae) have been used as a traditional medicine for the remedy of hemorrhage, blood stagnancy and thirstiness. To investigate phenolic compound from the node of Nelumbo nucifera, phytochemical isolation and structure elucidation were conducted. Four phenolic compounds were isolated from aqueous methanolic extract and the structure of these compounds were identised as (+)-catechin (1), (+)-gallocatechin (2), (+)-gallocatechin (4u-8)-catechin (3) and scolpoletin (4) respectively by the analysis of spectroscopic evidences and comparisions with the data of authentic samples.

• 생명과학회지
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• 제21권7호
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• pp.976-984
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• 2011

감잎으로부터 tyrosinase 억제물질의 추출을 위하여 60% ethanol을 사용하여 추출한 경우 추출물의 phenol성물질 함량이 21.91 mg/g로 가장 높았고, tyrosinase 억제효과도 37%의 억제율로 가장 높게 나타났다. 감잎 추출물로부터 tyrosinase 저해 활성 물질을 정제하기 위하여 Sephadex LH-20 column과 MCI-gel CHP-20 column을 이용하여 gradient로 용출한 결과 tyrosinase 저해 활성을 가지는 2가지 순수한 compound를 정제하였으며, 정제된 2가지 compound를 구조 동정한 결과, (+)-gallocatechin과 prodelphinidin B-3이었다. 정제물의 tyrosinase 저해활성을 측정한 결과 (+)-gallocatechin과 prodelphinidin B-3는 29.5, 40.2%의 저해활성을 나타내었으며, melanoma 세포에서의 멜라닌 생합성 저해효과를 측정한 결과 (+)-gallocatechin과 prodelphinidin B-3는 각각 32.5, 46.7%의 저해활성을 나타내어 멜라닌생성억제효과가 매우 뛰어남을 알 수 있었다. 정제된 미백물질을 이용하여 제조한 essence 제품의 안정성을 살펴본 결과, 미백 essence제품의 pH는 10,000 ppm의 농도에서 4.90~4.95였으며, 점도는 23,000~26,000 cP로 60일 동안의 저장기간 동안 큰 변화를 나타내지 않았다. 일반적 보존 시험인 온도에 따른 안정성과 광안정성(인공광, 자연광) 검사에서도 안정하였으며, 특수, 가혹 보존 시험인 온도순환에 따른 안정성 검사에서도 상의 분리와 변색, 변취 없이 모두 안정함을 확인할 수 있었다.

• 생약학회지
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• 제32권1호통권124호
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• pp.68-71
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• 2001

For the use of tannins in the whitening-effect cosmetics, inhibitory effect against tyrosinase activity was determined. Three condensed tannins including gallocatechin, gallocatechin 3',4'-di-O-gallate and epicatechin 3-O-gallate and three hydrolyzable tannins, 1,2,6-tri-O-galloyl-${\beta}$-D-glucose, 2,3-(S)-HHDP-D-glucose and pedunculagin showed 15-29% mild inhibitory effects against tyrosinase activity.

• 대한본초학회지
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• 제32권6호
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• pp.41-48
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• 2017

Objectives : This study was designed to estimate the antioxidative and neuroprotective effects of Torreyae Semen hot water extracts (TS). Methods : Torreyae Semen was extracted by hot water for 2 hours with a temperature of 105 degrees. Polyphenols and total flavonoid were measured and LC-MS/MS was used to certificate anticipated antioxidative compounds. The antioxidant activities of TS were measured as scavenging effects of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and Nitrite Oxides (NO). Cell viability and proliferation rate was measured MTT assay. The toxicities to thymocytes and splenocytes were evaluated by the proliferation rate of primary cultured cells of 7 weeks, male Balb/c mice. The antioxidant activities of TS on C6 mouse glioma cells were measured by the analysis of total glutathione contents variation. The neuroprotective effects against oxidative stresses were measured by MTT assay. Results : Polyphenols of TS was $92.00{\pm}1.24{\mu}g/mg$, and total flavonoids was $0.36{\pm}0.14{\mu}g/mg$. TS includes gallocatechin, epigallocatechin, gallocatechin gallate and epigallocatechin gallate. TS included gallocatechin, epigallocatechin, gallocatechin gallate, epigallocatechin gallate. TS showed DPPH and NO scavenging effects as dose-dependent manner at the concentrations of $0-10mg/m{\ell}$. In MTT assay, TS shows no significant toxicity to C6 cells, primary cultured thymocytes and splenocytes of Balb/c mice. TS increased the level of total glutathiones. TS increased cell viabilities of C6 cells against oxidative stresses such as $H_2O_2$, sodium nitroprusside (SNP), Rotenone at the concentrations of $0-0.063mg/m{\ell}$. Conclusions : TS shows the antioxidant and neuroprotecitive effects in these experiments.

• 약학회지
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• 제36권5호
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• pp.474-480
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• 1992

Astilbes rhizoma has been used for headache, arthralgia, chronic bronchitis and stomachalgia in traditional chinese medicine. The analgesic activities and their components of Astilbe chinensis var. davidii Rhizomes were evaluated. The ether and ethylacetate fractions of 70% EtOH extract showed considerable analgesic activities by acetic acid induced writhing method. Compound $1{\sim}5$ were isolated from ethylacetate fraction and identified as gallic acid, (+)-catechin, (+)-gallocatechin, bergenin and 11-O-galloylbergenin on the basis of spectroscopic methods. Among them (+)-gallocatechin showed stronger analgesic activity than that of other compounds.

• 생약학회지
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• 제27권1호
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• pp.1-5
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• 1996

Twelve compounds were isolated from stems of Securinega suffruticosa. On the basis of chemical and spectroscopic evidence, they were identified as gallic acid, corilagin, tercatain, geraniin, bergenin, norbergenin, 4-O-galloylnorberg-enin, 11-O-galloylnorbergenin, (+)-catechin, gallocatechin, isoquercitrin and rutin.

• 생약학회지
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• 제29권3호
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• pp.217-224
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• 1998

Eight compounds were isolated from the stem bark of Cornus walteri. On the basis of chemical and spectroscopic evidences, the structures of these compounds were identified as gallic acid, (+)-gallocatechin, (+)-catechin, quercetin, quercitrin, rutin, isoquercitrin and hyperoside respectively.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.78.2-78.2
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• 2003

We have previously reported that green tea catechins (GTC) displayed anti-thrombotic activity, and that this might be due to anti-platelet rather than anti-coagulation effects. In the present study, we have studied the anti-platelet activity and mechanism of gallocatechin gallate (GCG), which is a component of GTC. GCG inhibited the collagen- and U46619-induced aggregation of rabbit platelets, with IC$\^$50/ values of 63.0 and 48.3 ${\mu}$M, respectively. GCG also inhibited collagen-induced serotonin release and TxB$_2$ formation in a similar manner of platelets aggregation. (omitted)