• 약학회지
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• 제11권3_4호
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• pp.33-35
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• 1967

The internal structures of "Chunho", "Kangwhal" and "Dangui" and their original plants had been compared with each other in the prior report. Coumarin derivatives, khellactone from the roots of Angelica purpuraefolia $C_{HUNG}$ and decursin and decursinol from A. gigas $N_{AKAI}$ were also isolated. In this report, we made a simple and convenient identification method for the powdered drugs of several Angelica genus by using a thin layer chromatogrophic technique. The ehter extract of the powdered drugs were applied to a silica gel plate, and was developed with n-hexane and ethyl acetate (1:1) mixture. The characteristic U.V. absorption patterns of each drug were shown.

• 약학회지
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• 제11권3_4호
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• pp.22-26
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• 1967

From the root of Angelica gigas $N_{AKAI}$ (Umbelliferae), known as "Tang Gui" in Korea, substance A (colorless needle crystal, $C_{14}H_{14}O_{4}$, m.p. $178^{\circ}$) and B (white plate crystal, $C_{19}H_{20}O_{5}$, m.p. $111^{\circ}$) were obtained. Acetylation of "A" gave its monoacetate, white needles, $C_{16}H_{16}O_{5}$. Saponification of "B" with 5% sodium hydroxide produced "A" and senecionic acid. Examination using I.R. and N.M.R. spectra shows that "A" is identical with decursinol and that "B" with decursin. The root also appears to contain five coumarin derivatives and two steroidal compounds.

• 한국약용작물학회지
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• 제22권2호
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• pp.91-97
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• 2014

The contents of index components in 5 medicinal crops cultivated at different heights in Andong area. The contents of allantoin in Dioscorea batatas Decne and catalpol in Rehmannia glutinosa Liboschitz were not different by cultivation heights (100 ~ 200m). But the contents of index components in Astragalus membranaceus and Angelica acutiloba Kitagawa were higher in samples cultivated at elevated heights. At 700 m of heights, the contents of formononetin and astragaloside I in Astragalus membranaceus were 1.20 and $201.2{\mu}g/g$, respectively. The contents of decursin and nodakenin in Angelica acutiloba Kitagawa were 68.3 and 4.20 mg/g, respectively. Thus Astragalus membranaceus and Angelica acutiloba Kitagawa are recommended to be cultivated in higher altitude.

• 한국자원식물학회지
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• 제7권1호
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• pp.1-5
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• 1994

To decrease the bolting rate of Angelica gigas, The growth inhibitors such as MH, Pp333 (paclobutrazol) and CCC(chloroniequat or Cycocel) were treated twice with the interval of 20 days atthe forming stage of flower bud.Growth and bolting rate in plot treated inhibitors were retarded and decreased, and the yield ofroots was also decreased compared with non - treatment. The treatments of MH and Pp333 amongthree inhibitors showed the better effects in decreasing the bolting than that of CCC, but they did notshow the significant in the yield and contents of decursin among each of them.In order to improve the decreasing effects by chemicals, selection of suitable reagents, concentrationand number of treatment should be investigated.

• 대한화장품학회지
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• 제16권1호
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• pp.30-39
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• 1990

Angelica gigas extract and Cnidium of ficinale extract in Bath essence were determined by high performance liquid chromatography with a UV detector by use of Bondapak C-18 column. Decursin and Ligustilide were used as indicator ingredient for analysis of Angelica gigas extract and Cnidium of ficinale extract. Each indicator ingredient was isolated from each plant and purified using silver-ni-trate treated silica column. Their structure were conformed with IR, NMR and HPLC. The recoveries of Angelica gigas extract and Cnidium officinale extract added to Bath essence sample were 96.5-109.5% and 112-115%.

• 한국자원식물학회지
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• 제32권2호
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• pp.116-123
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• 2019

본 연구는 참당귀 뿌리의 가공 과정을 통해 분류되는 신, 대미, 중미, 세미 부위에서 지표성분과 항산화 활성을 분석함으로써, 약재로 이용되지 않는 세미 부위의 부산물 활용가능성을 평가하기 위해 수행하였다. 참당귀 뿌리의 가공 시 세미 부위의 회수율은 100kg당 $15{\pm}1.0%$로 비교적 많은 부분을 차지하였다. 지표성분 함량은 참당귀 뿌리의 직경이 작을수록 증가하는 경향성을 나타내었으며, 세미 부위에서 decursin ($4.42{\pm}0.27%$)과 decursinol angelate ($4.46{\pm}0.28%$)의 함량이 가장 높았다. 항산화 활성도 뿌리의 직경이 작아질수록 증가하는 경향성을 나타내었으며, 세미 부위의 항산화 활은 유통되는 한약재와 유사하거나 우수하였다. 세미의 지표성분 함량 및 항산화 활성의 우수성과 회수율을 고려하면, 참당귀 부산물인 세미를 건강기능성 식품, 화장품 등의 추출물 원료로 활용이 가능할 것으로 생각된다.

• Journal of Applied Biological Chemistry
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• 제63권1호
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• pp.29-33
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• 2020

한약재의 불법 유통을 방지하기 위해 신속 정확한 원산지 판별방법 개발이 필요함에 따라 TDU-GC/MS를 이용하여 한국품종 당귀와 외국품종 당귀를 판별함과 동시에 한국품종 당귀의 재배지가 한국인지를 판별할 수 있는 기법을 연구하였다. 당귀추출물을 열탈착 시킨 후 냉각응축시스템에서 응축시켜 일시에 GC/MS로 분석한 결과 국산 품종 당귀(참당귀)는 TIC의 RT 26.9-27.2에서 coumarin 유도체인 decursin과 decursinol peak가 확인되었다. 중국 품종 당귀(중당귀)의 경우 RT 17.2 부근에서 ligustilide의 peak가 검출되었다. 국산 품종 참당귀 원산지에 따른 휘발성분의 차이를 알아보기 위하여 twister로 흡착하여 TDU-GC/MS로 m/z 40-400 amu 범위에서 mass spectrum을 측정하였다. 참당귀의 국내 및 중국 재배 시료 TIC는 전반적으로 같은 경향을 냈으나 TIC를 부분 scan한 결과 RT 15.4-16.1에서 국내 및 중국 재배의 peak pattern 차이를 확인할 수 있었다. Peak A (RT 15.54)과 B (RT 16.05)의 비율은 국내 재배는 0.0-0.2, 중국 재배의 경우 0.5-2.8으로서 TDU-GC/MS의 TIC peak pattern 비교를 통한 원산지 판별 가능성을 확인하였다.

• 한국자원식물학회지
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• 제31권4호
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• pp.312-321
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• 2018

Angelica tenuissima, also known as Ligusticum tenuissimum, is classified as a food-related plant and has been used as traditional medicines treating headache and anemia in Asia. However, its anti-melanogenic effect has not been reported in detail. When the extract of Angelica tenuissima (ATE) was prepared by the extraction with 70% EtOH at $80^{\circ}C$ (final yield = 22%), the contents of decursin and Z-ligustilide in ATE were determined 0.06% and 8.43%, respectively. Total flavonoid and phenolic content in mg ATE were $5.52{\pm}0.07{\mu}g$ quercetin equivalents and $237.27{\pm}13.24{\mu}g$ gallic acid equivalents, respectively. Antioxidant capacity of ATE determined by DPPH and ABTS assay was increased with a dose dependent manner up to $1000{\mu}g/m{\ell}$. The amount of melanin synthesis followed by ${\alpha}-melanocyte$ stimulating hormone on B16F10 cells were significantly reduced in the presence of ATE (250 to $1000{\mu}g/m{\ell}$, p<0.05). ATE (125 to $1000{\mu}g/m{\ell}$, p<0.05) suppressed the tyrosinase activity but did not show any significant effect on ${\alpha}-glucosidase$ activity at the same condition. Taken together, ATE possesses tyrosinase inhibitory potential with significant antioxidant capacities. These effects of ATE might be involved in suppression of melanin synthesis, at least, in B16F10 cells. The anti-melanogenic potential of ATE will provide an insight into developing a new skin whitening product.

• 한국토양비료학회지
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• 제48권5호
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• pp.421-427
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• 2015

Angelica gigas Nakai is one of the most widely used herbal medicines and is known to have many pharmaceutical effects including an anti-oxidant, anti-cancer etc. This study was carried out to investigate an effect of fertilization on leaf yield, production of dry-matter and accumulation of pyranocurmarine compounds such as decursin (DE) and decursinol angelate (DA) in Angelica gigas Nakai. Effect of fertilization was determined from response surface regression equation composing of 2 by 3 factorial arrangement of urea, sodium dihydrogen phosphate and potassium chloride. Yield of leaf in Angelica gigas Nakai significantly increased until 100 days after transplanting. Production of leaf also tended to increase with increasing nitrogen fertilization. Model of regression equation showed that leaf production depended upon nitrogen ($Pr>{\mid}t{\mid}$ : 0.087, 0.256 and 0.079). Also, statistical results between nitrogen application level and production of dry-matter showed significant relationship (p<0.05) and contents of dry-matter was highest in 10 kg 10a-1 treatment on 24 Sep. Active compound isolated and purified from leaf and root of Angelica gigas Nakai was identified as DE and DA by gas chromatograph-mass spectrophotometry (GC-MS). Concentration of DA as prevalent compound in leaf was highest on 20 Aug. but decreased on 24 Sep. Amount of DE and DA accumulated in Angelica gigas Nakai significantly increased with growth stages and nitrogen level. The result of our investigation imply that nitrogen fertilization is important factor for production of leaf and accumulation of pyranocurmarine in Angelica gigas Nakai as a medicinal/food materials.

• 한국자원식물학회지
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• 제32권1호
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• pp.9-18
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• 2019

점봉산 참당귀는 해발 750-965 m의 교목층은 들메나무, 고로쇠나무, 신갈나무가 분포하고, 아교목층에는 당단풍, 까치박달나무가 분포하는 계곡지로 유기물이 집적되기 쉬운 다습한 지형에 자생하였다. 참당귀 군락의 토양 분석 결과 토양pH는 4.9-6.0으로 우리나라 산림토양의 평균과 매우 유사한 값을 보였으며, 토양 양분의 지표인 유기물함량, 전질소 양이온치환용량, 치환성 Ca, K, Mg, Na의 함량은 산림토양의 평균보다 비교적 높은 값을 보였다. 생장 특성으로는 9월 말에는 뿌리 생장이 평균47.9 g이나 10월 말에는 33.8 g으로 29% 감소하였으며, 유용 성분은 반대로 10월말에 decursin 3.8%, decursinol angelate 3.6%로 각각 26%, 67% 증가하였다. 참당귀 자생지 8개 집단의 생장 특성 및 유용성분 함량과 토양특성 간의 상관분석을 수행한 결과 뿌리 생장은 치환성 양이온인 K, Mg, Na과 높은 양의 상관관계를 보였고, 유용성분은 뿌리의 수분함량과 높은 음의 상관관계를 보였다.