• YAKHAK HOEJI
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• v.11 no.3_4
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• pp.33-35
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• 1967

The internal structures of "Chunho", "Kangwhal" and "Dangui" and their original plants had been compared with each other in the prior report. Coumarin derivatives, khellactone from the roots of Angelica purpuraefolia $C_{HUNG}$ and decursin and decursinol from A. gigas $N_{AKAI}$ were also isolated. In this report, we made a simple and convenient identification method for the powdered drugs of several Angelica genus by using a thin layer chromatogrophic technique. The ehter extract of the powdered drugs were applied to a silica gel plate, and was developed with n-hexane and ethyl acetate (1:1) mixture. The characteristic U.V. absorption patterns of each drug were shown.

• YAKHAK HOEJI
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• v.11 no.3_4
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• pp.22-26
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• 1967

From the root of Angelica gigas $N_{AKAI}$ (Umbelliferae), known as "Tang Gui" in Korea, substance A (colorless needle crystal, $C_{14}H_{14}O_{4}$, m.p. $178^{\circ}$) and B (white plate crystal, $C_{19}H_{20}O_{5}$, m.p. $111^{\circ}$) were obtained. Acetylation of "A" gave its monoacetate, white needles, $C_{16}H_{16}O_{5}$. Saponification of "B" with 5% sodium hydroxide produced "A" and senecionic acid. Examination using I.R. and N.M.R. spectra shows that "A" is identical with decursinol and that "B" with decursin. The root also appears to contain five coumarin derivatives and two steroidal compounds.

• Korean Journal of Medicinal Crop Science
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• v.22 no.2
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• pp.91-97
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• 2014

The contents of index components in 5 medicinal crops cultivated at different heights in Andong area. The contents of allantoin in Dioscorea batatas Decne and catalpol in Rehmannia glutinosa Liboschitz were not different by cultivation heights (100 ~ 200m). But the contents of index components in Astragalus membranaceus and Angelica acutiloba Kitagawa were higher in samples cultivated at elevated heights. At 700 m of heights, the contents of formononetin and astragaloside I in Astragalus membranaceus were 1.20 and $201.2{\mu}g/g$, respectively. The contents of decursin and nodakenin in Angelica acutiloba Kitagawa were 68.3 and 4.20 mg/g, respectively. Thus Astragalus membranaceus and Angelica acutiloba Kitagawa are recommended to be cultivated in higher altitude.

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.1-5
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• 1994

To decrease the bolting rate of Angelica gigas, The growth inhibitors such as MH, Pp333 (paclobutrazol) and CCC(chloroniequat or Cycocel) were treated twice with the interval of 20 days atthe forming stage of flower bud.Growth and bolting rate in plot treated inhibitors were retarded and decreased, and the yield ofroots was also decreased compared with non - treatment. The treatments of MH and Pp333 amongthree inhibitors showed the better effects in decreasing the bolting than that of CCC, but they did notshow the significant in the yield and contents of decursin among each of them.In order to improve the decreasing effects by chemicals, selection of suitable reagents, concentrationand number of treatment should be investigated.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.16 no.1
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• pp.30-39
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• 1990

Angelica gigas extract and Cnidium of ficinale extract in Bath essence were determined by high performance liquid chromatography with a UV detector by use of Bondapak C-18 column. Decursin and Ligustilide were used as indicator ingredient for analysis of Angelica gigas extract and Cnidium of ficinale extract. Each indicator ingredient was isolated from each plant and purified using silver-ni-trate treated silica column. Their structure were conformed with IR, NMR and HPLC. The recoveries of Angelica gigas extract and Cnidium officinale extract added to Bath essence sample were 96.5-109.5% and 112-115%.

• Korean Journal of Plant Resources
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• v.32 no.2
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• pp.116-123
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• 2019

Angelica gigas Nakai (AGN) is a traditional medicinal herb especially in Korea. It contains pyranocoumarins, which are major active components including decursin (D) and decursinol angelate (DA). This study was carried out to determine the change in active component content and antioxidant activity depending on the root diameter of AGN. Several processing steps are involved to use AGN roots as medicine. The dried AGN roots are divided into body (B), thick root (TkR), medium root (MR) and thin root (TnR) according to their diameter before cutting into medicine. The recovery rates of each root parts per 100 kg were measured as $32.3{\pm}2.5$, $9.0{\pm}1.0$, $39.3{\pm}2.1$ and $15.0{\pm}1.0%$, respectively and the mean diameters were measured as $51.95{\pm}4.55$, $7.05{\pm}0.89$, $2.88{\pm}0.49$ and $1.57{\pm}0.32mm$. Two index components, D and DA, were analyzed. The change of both D and DA content showed a similar tendency. Both D and DA content were increased as the root diameter decreased (higher in TnR). In addition, antioxidant activity was higher in B and TnR, and lower in TkR and MR than control. This study showed that the thinner the root diameter, the higher the D and DA content in AGN roots and that TnR has excellent antioxidant activity compared to other root parts, suggesting that the thinner root part of AGN could be used as a potential material.

• Journal of Applied Biological Chemistry
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• v.63 no.1
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• pp.29-33
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• 2020

The objective of this study was to develop a fast and accurate method of variety discrimination and geographical discrimination origin of Korean angelica (Korean variety, Angelica gigas Nakai) by using TDU-GC/MS. Two peaks of decursin and decursinol, which are coumarin derivatives were identified in the range of Total Ion Chromatogram (TIC) RT 26.9-27.2 of the Korean angelica by GC/MS analysis at the time of condensation in a refrigerated condensation system after thermal desorption of sample extracts. In case of Chinese angelica (Chinese variety), ligustilide peak was detected at the RT 17.2. In order to investigate the difference of volatile components according to the geographical origin of Korean variety, the mass spectra were measured by TDU-GC/MS at the range of m/z 40-400 amu. The TIC of domestic cultivation and Chinese cultivation of the Korean variety, Angelica gigas Nakai showed the same tendency as a whole. However, in partial scans of TIC, two peaks detected at 15.54 and 16.05 of RT showed different peak patterns between Korean angelica (Korean variety) cultivated in Korea and in China. The ratio of Peak A (RT 15.54) and B (RT 16.05) was 0.0-0.2 for domestic cultivation and 0.5-2.8 for Chinese cultivation, confirming the possibility of discriminating origin by comparing the TIC peak pattern of TDU-GC/MS.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.312-321
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• 2018

Angelica tenuissima, also known as Ligusticum tenuissimum, is classified as a food-related plant and has been used as traditional medicines treating headache and anemia in Asia. However, its anti-melanogenic effect has not been reported in detail. When the extract of Angelica tenuissima (ATE) was prepared by the extraction with 70% EtOH at $80^{\circ}C$ (final yield = 22%), the contents of decursin and Z-ligustilide in ATE were determined 0.06% and 8.43%, respectively. Total flavonoid and phenolic content in mg ATE were $5.52{\pm}0.07{\mu}g$ quercetin equivalents and $237.27{\pm}13.24{\mu}g$ gallic acid equivalents, respectively. Antioxidant capacity of ATE determined by DPPH and ABTS assay was increased with a dose dependent manner up to $1000{\mu}g/m{\ell}$. The amount of melanin synthesis followed by ${\alpha}-melanocyte$ stimulating hormone on B16F10 cells were significantly reduced in the presence of ATE (250 to $1000{\mu}g/m{\ell}$, p<0.05). ATE (125 to $1000{\mu}g/m{\ell}$, p<0.05) suppressed the tyrosinase activity but did not show any significant effect on ${\alpha}-glucosidase$ activity at the same condition. Taken together, ATE possesses tyrosinase inhibitory potential with significant antioxidant capacities. These effects of ATE might be involved in suppression of melanin synthesis, at least, in B16F10 cells. The anti-melanogenic potential of ATE will provide an insight into developing a new skin whitening product.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.5
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• pp.421-427
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• 2015

Angelica gigas Nakai is one of the most widely used herbal medicines and is known to have many pharmaceutical effects including an anti-oxidant, anti-cancer etc. This study was carried out to investigate an effect of fertilization on leaf yield, production of dry-matter and accumulation of pyranocurmarine compounds such as decursin (DE) and decursinol angelate (DA) in Angelica gigas Nakai. Effect of fertilization was determined from response surface regression equation composing of 2 by 3 factorial arrangement of urea, sodium dihydrogen phosphate and potassium chloride. Yield of leaf in Angelica gigas Nakai significantly increased until 100 days after transplanting. Production of leaf also tended to increase with increasing nitrogen fertilization. Model of regression equation showed that leaf production depended upon nitrogen ($Pr>{\mid}t{\mid}$ : 0.087, 0.256 and 0.079). Also, statistical results between nitrogen application level and production of dry-matter showed significant relationship (p<0.05) and contents of dry-matter was highest in 10 kg 10a-1 treatment on 24 Sep. Active compound isolated and purified from leaf and root of Angelica gigas Nakai was identified as DE and DA by gas chromatograph-mass spectrophotometry (GC-MS). Concentration of DA as prevalent compound in leaf was highest on 20 Aug. but decreased on 24 Sep. Amount of DE and DA accumulated in Angelica gigas Nakai significantly increased with growth stages and nitrogen level. The result of our investigation imply that nitrogen fertilization is important factor for production of leaf and accumulation of pyranocurmarine in Angelica gigas Nakai as a medicinal/food materials.

• Korean Journal of Plant Resources
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• v.32 no.1
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• pp.9-18
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• 2019

This study was carried out to investigate characteristics of growth and active compounds of Angelica gigas population distributed in Mt. Jeombong. Quadrates were established in native area to harvest root part of Angelica gigas and analyze soil properties in September and October 2017. It was found that Angelica gigas populations were collected above the altitude 758 m near the valley covered with Fraxinus mandshurica and Acer pictum, and so on. In case of soil nutrient, soil organic matter, available phosphorous, and total nitrogen ranged 5.8-25.2%, 23.0-67.9 mg/kg and 0.3-1.3% respectively. Also, the Fresh weight of root ranged 28.8-65.3 g in September and 22.3-75.6 g in October. The content of active compounds ranged 2.7-4.7% in decursin and 2.9-4.5% decursinol angelate in October that was increased by 1% and 2.4% respectively compared with that in September. In the final analysis, there was positive correlation between fresh weight of root and exchangeable cation (natrium, calcium, magnesium). Also, there was positive correlation between active compounds and the content of water in root part of Angelica gigas.