• 한국식품위생안전성학회지
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• 제32권3호
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• pp.222-227
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• 2017

연구는 대기압 유전체장벽방전 플라즈마 처리에 따른 식품유해 미생물 사멸효과를 조사하기 위해 수행되었다. 플라즈마 처리 시, 활성종 생성 및 농도에 영향을 미치는 노출시간, 노출거리, 산소비율, 전력 변화에 따른 E. coli의 사멸효과를 조사한 결과, E. coli의 사멸율은 플라즈마 처리를 위한 노출시간, 산소비율, 전력의 증가에 따라 증가한 반면, 노출거리의 증가에 따라서는 사멸율이 감소하였다. 이 결과는 미생물 시료가 플라즈마에 노출되는 시간이 증가됨으로서 시료 내 NO 농도가 증가되고, E. coli의 사멸율 역시 증가되는 결과로 뒷받침할 수 있고, 미생물 사멸효과를 높이기 위해서는 활성종의 농도가 증가되어야 함을 의미한다. E. coli와 함께 B. cereus, B. subtilis, B. thuringiensis, B. atrophaeus를 대상으로 대기압 유전체 장벽방전 플라즈마에 의한 살균효과를 조사한 결과, 72.3~91.3%의 높은 사멸율을 나타내었다. 이러한 결과로 미루어, 대기압 유전체장벽방전 플라즈마기술은 다양한 미생물에 적용될 수 있는 유용한 살균기술임을 확인하였다.

• KSBB Journal
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• 제23권3호
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• pp.205-212
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• 2008

본 연구의 목적은 위치특이적 펩타이드 및 단백질을 사용하여 재조합 대장균에서 활성형 인간 상피세포성장인자(hEGF)를 고효율로 발현할 수 있는 방법을 찾아내는 데 있다. 재조합 대장균내 cytoplasm 및 periplasm 영역에서 hEGF의 발현을 위해 각각 세개의 응합 펩타이드 및 단백질을 선정하여 상호 비교하였다. 재조합 대장균에서 hEGF의 발현유도시 대부분 불용성 단백질로 생산되는 현상을 극복하기 위해 cytoplasm영역에서는 ATS, thioredoxin, 리파제를 융합파트너로 사용하였으며 periplasm 영역에서는 foldase인 DsbA와 DsbC, 용융성 고발현 단백질인 maltose binding protein을 선택하여 사용하였다. Periplasm영역에서 발현유도를 시키는 융합단백질의 경우 cytoplasm영역에서의 발현양도 용융성 형태로 고발현 되는 것을 알 수 있었으며 전체적으로 약 2배가량의 용융성 형태로 발현되었다. hECF의 발현율을 가장 높일 수 있는 융합단백 질은 maltose binding protein이었으나 발현된 융합단백질의 24%가 불용성 단백질로 형성되어 활성형 형태로 얻는 데 한계가 있었으며, 활성형 형태로 hEGF의 발현을 위해서는 DsbA를 응합단백질로 사용한 경우에 18.1 mg/L로 가장 높은 발현농도를 보였다. Cytoplasm 영역에서 발현유도를 한 경우에는 ATS와 thioredoxin을 응합파트너로 hEGF를 발현한 경우 용융성 형태로 높은 발현율을 보였다. 특히 ATS와 같은 펩타이드를 N-말단에 융합시킨 경우 불용성을 방지하는 효과를 보여 이황화결합의 불완전성이나 소수성으로 인해 불용성 단백질로 발현되는 기존의 단백질을 활성형 형태로 발현하는데 될 수 있음을 확인할 수 있었다.

• 한국산학기술학회논문지
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• 제16권5호
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• pp.3491-3498
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• 2015

본 연구는 건축 도시 분야의 시장 전망과, 건축 도시 상품 패러다임 변화, 융복합 교육수요 증가에 기초하여 경력자 실무자들을 대상으로 융복합 교육 수요, 교육방법, 교육과정 운영체계 등에 대한 요구를 파악하는데 목적을 두었다. 건축 도시분야의 융복합 교육에 대한 수요자의 전반적인 요구 식별을 위해, 건설분야와 비건설분야 재직자 실무자를 대상으로 온라인 설문을 실시하였다. 교육수요 조사결과는 세 가지로 요약될 수 있다. 첫 번째, 단기적 관점에서 실무환경과 밀접한 연계성을 가진 학문(세부전공 빈도증가율: 금융(716.7%), 경영(633.3%), 기획 및 마케팅(454.5%))과 기존 학문을 융합하는 것이 중요하다. 두 번째, 융복합 교육에 적합한 운영방식에 대한 설문결과가 온 오프라인을 병행해야 한다는 의견이 79%를 차지했다. 이에 따라 오프라인 대학과 사이버 대학의 장점을 상호 보완한 새로운 교육방법이 요구된다. 세 번째, 융복합 교과과정내 교육수요자의 교과과목 선택방식은 한정 자유형이 70.9%로 나타났다. 교육과정 운영체계는 건설 실무의 흐름에 맞추어 직무관련 교과과목을 배치하고, 융복합 교과과목은 선택적으로 수강할 수 있게 구성하는 것이 필요하다.

• 한국측량학회지
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• 제34권2호
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• pp.121-132
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• 2016

This study proposes an approach for simulating high spatial resolution satellite images acquired under arbitrary sun-sensor geometry using existing images and 3D (three-dimensional) data. First, satellite images, having significant differences in spectral regions compared with those in the simulated image were transformed to the same spectral regions as those in simulated image by using the UPDM (Universal Pattern Decomposition Method). Simultaneously, shadows cast by buildings or high features under the new sun position were modeled. Then, pixels that changed from shadow into non-shadow areas and vice versa were simulated on the basis of existing images. Finally, buildings that were viewed under the new sensor position were modeled on the basis of open library-based 3D reconstruction program. An experiment was conducted to simulate WV-3 (WorldView-3) images acquired under two different sun-sensor geometries based on a Pleiades 1A image, an additional WV-3 image, a Landsat image, and 3D building models. The results show that the shapes of the buildings were modeled effectively, although some problems were noted in the simulation of pixels changing from shadows cast by buildings into non-shadow. Additionally, the mean reflectance of the simulated image was quite similar to that of actual images in vegetation and water areas. However, significant gaps between the mean reflectance of simulated and actual images in soil and road areas were noted, which could be attributed to differences in the moisture content.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.187-192
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• 2010

In order to monitor the possibility of horizontal gene transfer between transgenic rice and microorganisms in a paddy rice field, the gene flow from a bifunctional fusion (TPSP) rice containing trehalose-6-phosphate synthase and phosphatase to microorganisms in soils was investigated. The soil samples collected from the paddy rice field during June 2004 to March 2006 were investigated by multiplex PCR, Southern hybridization, and amplified fragment length polymorphism (AFLP). The TPSP gene from soil genomic DNAs was not detected by PCR. Soil genomic DNAs did not show homologies on the Southern blotting data, indicating that gene transfer did not occur during the last two years in the paddy rice field. In addition, the AFLP band patterns produced by soil genomic DNAs from both transgenic and non-transgenic rice fields appeared similar to each other when analyzed by the NTSYSpc program. Thus, these data suggest that transgenic rice does not give a significant impact on the communities of soil microorganisms, although long-term observation may be needed.

• Nuclear Engineering and Technology
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• 제38권8호
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• pp.785-792
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• 2006

The microwave heating system of KSTAR consists of ECH and LHCD. ECH and LHCD offer the benefits ofa reliable operation at the start of plasma formation and a non-inductive current drive durable steady state operation, respectively. LHCD uses a C-band microwave system with a frequency of 5 GHz. A pulsed power modulator with a power of 3.6 MW, $4{\mu}S$, 200 pps is required to drive the high-powered magnetron. The development of a pulse modulator with 1:4 pulse transformers is the focus of the research in this study. The peak power handling capability is 3.6 MW (45 kV, 90 A at load side with a pulse width of $4{\mu}S$). This paper describes the system overview and test results of the pulsed modulator. In particular, a simulated waveform is compared with the tested waveform.

• 한국재료학회지
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• 제20권1호
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• pp.19-24
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• 2010

The current study examined Ag migration from the Ag paste bump in the SABiT technology-applied PCB. A series of experiments were performed to measure the existence/non-existence of Ag in the insulating prepreg region. The average grain size of Ag paste was 30 nm according to X-ray diffraction (XRD) measurement. Conventional XRD showed limitations in finding a small amount of Ag in the prepreg region. The surface morphology and cross section view in the Cu line-Ag paste bump-Cu line structure were observed using a field emission scanning electron microscope (FE-SEM). The amount of Ag as a function of distance from the edge of Ag paste bump was obtained by FE-SEM with energy dispersive spectroscopy (EDS). We used an electron probe micro analyzer (EPMA) to improve the detecting resolution of Ag content and achieved the Ag distribution function as a function of the distance from the edge of the Ag paste bump. The same method with EPMA was applied for Cu filled via instead of Ag paste bump. We compared the distribution function of Ag and Cu, obtained from EPMA, and concluded that there was no considerable Ag migration effect for the SABiT technology-applied printed circuit board (PCB).

• Asian-Australasian Journal of Animal Sciences
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• 제21권5호
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• pp.648-656
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• 2008

The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro development of somatic cell nuclear transfer (SCNT) embryos. Green fluorescent protein (GFP) gene was used as a foreign gene and a non-transfected somatic cell was utilized as a control karyoplast. Monolayers of porcine cells were established and subsequently transfected with a GFP-expressing gene (pEGFP-N1) using three types of transfection reagents (LipofectAMINE PLUS, FuGENE 6 or ExGen500). Donor cells used for SCNT included transfected fetal or adult fibroblasts and oviduct epithelial cells, either serum-fed or serum-starved. Oocytes matured in vitro for 42 h were reconstructed with either transfected or non-transfected porcine somatic cells by electric fusion and activation using a single DC pulse of 1.8 kV/cm for $30{\mu}s$ in $Ca^{2+}$ and $Mg^{2+}-containing$ 0.26 M mannitol solution. Reconstructed oocytes were subsequently cultured in NCSU-23 medium for 168 h and the developmental competence and cell number in blastocyst were compared. There were no significant differences (P>0.05) in fusion, cleavage rates or development to the blastocyst stage between non-transfected, transfected, serum-fed and serum-starved cells. However, the rates of GFP-expressing blastocysts were higher in the FuGENE 6 group (71.4%) among transfection reagents and in the fetal fibroblasts group (70.4%) for donor cells. These results indicate that fetal fibroblasts transfected with FuGENE 6 can be used as donor cells for porcine SCNT and that GFP gene can be safely used as a marker of foreign genes in porcine transgenesis.

• Journal of Advanced Marine Engineering and Technology
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• 제30권7호
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• pp.791-799
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• 2006

The aim of this paper is to design an adaptive speed control system of a marine diesel engine by fusion of hard computing based proportional integral derivative (PID) control and soft computing based fuzzy control methods. The model of a marine diesel engine is considered as a typical non oscillatory second order system. When its model and the actual marine diesel engine ate not matched, it is hard to control the speed of the marine diesel engine. Therefore, this paper proposes two methods in order to obtain the speed control characteristics of a marine diesel engine. One is an efficient method to determine the PID control parameters of the nominal model of a marine diesel engine. Second is a reference adaptive speed control method that uses a fuzzy controller and derivative operator for tracking the nominal model of the marine diesel engine. It was found that the proposed PID parameters adjustment method is better than the Ziegler & Nichols' method, and that a model reference adaptive control is superior to using only PID controller. The improved control method proposed here, could be applied to other systems when a model of a system does not match the actual system.

• Journal of Microbiology and Biotechnology
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• 제25권1호
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• pp.119-126
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• 2015

Among the various human growth factors, epidermal growth factor (hEGF, consisting of 53 amino acids) has various effects on cell regeneration, stimulation of proliferation, migration of keratinocytes, formation of granulation tissues, and stimulation of fibroblast motility, which are important for wound healing. Owing to their multiple activities, EGFs are used as pharmaceutical and cosmetic agents. However, their low productivity, limited target specificity, and short half-life inhibit their application as therapeutic agents. To overcome these obstacles, we fused the collagen-binding domain (CBD) of Vibrio mimicus metalloprotease to EGF protein. About 18 or 12 amino acids (aa) (of the 33 total amino acids), which were essential for collagen-binding activity, were combined with the N- and C-termini of EGF. We constructed, expressed, and purified EGF (53 aa)-CBD (18 aa), EGF (53 aa)-CBD (12 aa), CBD (18 aa)-EGF (53 aa), and CBD (12 aa)-EGF (53 aa). These purified recombinant proteins increased the numbers of cells in treated specimens compared with non-treated specimens and control hEGF samples. The collagen-binding activities were also evaluated. Furthermore, CBD-hybridized hEGF induced phosphorylation of the EGF receptor. These results suggested that these fusion proteins could be applicable as small therapeutic agents in wound tissue healing.