• The Korean Journal of Community Living Science
• /
• v.18 no.2
• /
• pp.293-300
• /
• 2007

The antimicrobial activity of 10 different traditional medicinal herbs extracts against E. coli was determined using paper disc method. Caesalpinia sappan and Rhus javanica, extracts in 5 mg/ml, and Paeonia suffruticosa and Seutellaria baicalensis extracts in 10 mg/ml showed significant antimicrobial activity against E. coli. Minimum inhibitory concentrations of medicinal herbs extracts were in the range of $1.4{\sim}8mg/ml\;and\;1.2{\sim}12mg/ml$, for MeOH extracts and EtOH extracts, respectively. In addition, the antimicrobial activity of each solvent fraction was most significant with the EtOAc layer. Optical density at 620nm after 24 hours incubation of E. coli in the presence of 100, 300 or 500 ppm of Caesalpinia sappan extract ranged from 0.02 to 0.1 compared to 0.6 in the absence of Caesalpinia sappan extract, indicating that growth of E. coli was significantly inhibited within 24 hours by the addition of at least 300 ppm of Caesalpinia sappan extract. Optical density at 620 nm after 24 hours incubation of E. coli in the presence of 300 ppm of Rhus javanica extract ranged from 0.02 to 0.2 compared to 0.5 in the absence of Rhus javanica extract, indicating that growth of E. coli was also significantly inhibited within 24 hours by the addition of at least 500 ppm of Rhus javanica extract. In conclusion, these findings suggest that extracts from medicinal herbs may play important roles in antimicrobial activities against E. coli.

• 대한임상약리학회:학술대회논문집
• /
• 1997.12a
• /
• pp.24-24
• /
• 1997

• Biomolecules & Therapeutics
• /
• v.9 no.4
• /
• pp.249-257
• /
• 2001

The effect of oxidative stress on the alterations of different antioxidant enzyme activities was investigated in human skin melanoma cell line (SK-MEL-2). Oxidative stress was induced by the exposure to hydrogen peroxide ($H_2O$$_2$). SK-MEL-2 cells were treated with antioxidants such as vitamin E and selenomethionine in combination with $H_2O$$_2$. SK-MEL-$_2$ cells were exposed to various concentrations of $H_2O$$_2$ and measured the time course of changes in cell viability and antioxidant enzyme activities for 24 hr. Oxidative stress was induced by the exposure to 2.5mM hydrogen peroxide ($H_2O$$_2$) resulted in declining significantly for 24 hr. GPX and CAT activities peaked at 3 hr and returned to control levels by 24 hr. On the contrary, SOD activity was inactive before 6 hr but recovered at 24 hr. In case vitamin E (Vit E) and selenomethionine (Se-Met) were used at nontoxic concentrations (25$\mu$M Vit E/500$\mu$M Se-Met) to oxidative stress was induced by the exposure to hydrogen peroxide ($H_2O$$_2$) led to a 3- and 5-fold increase on the viability comparing to control and caused an increase in GPX activity respectively.

• Journal of the Korean Wood Science and Technology
• /
• v.47 no.5
• /
• pp.587-596
• /
• 2019

This study aimed to determine the antimalarial effect of the Strychnos ligustrina (SLW) wood extracts and to analyze its phytochemicals. The SLW powder samples were macerated with 100% ethanol (E100), 75% ethanol (E75), 50% ethanol (E50), 25% ethanol (E25), and aqueous (A100). The extracts were analyzed by LCMS/MS, and its in-vitro antimalarial activity was tested with Plasmodium falciparum. The results showed that the extract yields of E100, E75, E50, E25, and A100 were 4.3, 5.2, 5.3, 4.7, and 3.6%, respectively. The antimalarial activities of the A100, E25, E50, and E75 extracts were classified as active with $IC_{50}$ values of 38.6, 42.6, 42.9, and $43.7{\mu}g/mL$, respectively. But, the antimalarial activity of the E100 extract was classified as slightly active with $IC_{50}$ values of $87.4{\mu}g/mL$. The dominant compounds contained in the extracts of A100, E25, E50, E75, and E100 was the alkaloid compound, namely brucine with relative concentrations of 24.96, 24.55, 21.33, 11.79, and 11.62%, respectively.

• Journal of Plant Biology
• /
• v.38 no.4
• /
• pp.399-407
• /
• 1995

The effects of 0.5 and 1.0 ppm of ozone on the developing chloroplast of barley (Hordeum vulgare L) seedling during greening were investigated by PSI and II activities, chlorophyll fluorescence, and the contents of chlorophyll. Etiolated barley seedling was treated 0.5 and 1.0 ppm ozone for the first 4 h during greening. In 24 h greening experiment, the contents of chlorophyll were decreased by increasing ozone concentration from 0.5 ppm to 1.0 ppm. In 24 h greening experiment, Fo, Fv, Fm and qE were greatly decreased as the concentration of ozone was increased, but those were not considerably decreased in 48 h greening experiment. In another experiment, the developing barley seedling was treated with 0.5 and 1.0 ppm ozone for the last 4 h during greening period, which was 24 h or 48 h. In both experiments the PS II activity was decreased as the concentration of ozone was increased, but not in PS I activity. Fv, Fm and Fv/Fo were also decreased as the concentration of ozone was increased. qP and qR were strikingly decreased as the concentration of ozone was increased in both experiments.iments.

• Journal of Haehwa Medicine
• /
• v.4 no.1
• /
• pp.357-371
• /
• 1995

We have completed a study to measure the contents of glucose, BUN, creatinine. LDH, and T-protein with respect to a fatigued condition in the bloods of rats which a constant swimming is loaded and to measure the maximun swimming time of mice The test has been carried out as a part of the basic study on the efficacy of B. E. P. (Biological Energy Projector) for emitting a light energy having a specific wavelength out of far-infrared rays. As a result. We have reached the following conclusions: 1. At testing of mice's maximun swimming time, all of B.E.P.(2. 4. 8. 24hrs) treated group have been increased in comparison with the control group, but only 24hrs-B.E.P. treated group significantly increased during 4 weeks. 2. The contents of glucose, BUN. creatinine, LDH, and T-protein measured immediately after the swimming of mice have been distinctly changed but not been significantly changed at their increase and decrease in comparison with the control group. 3. At 3rd day out of the swimming loading, the contents of glucose in the blood serum of the white rat have been distinctly increased in comparison with the control group. And 24hrs-B.E.P treated group surpassed 8hrs-B.E.P. treated group. 4. At 1st and 3rd day, the contents of creatinine in the blood serum of the white rat have been distinctly increased at B.E.P. (8, 24hrs) treated groups in comparison with the control group and have been recovered to the condition of the normal group. 5. After three days, the contents of BUN in the blood serum of the white rat have been significantly decreased in B.E.P.(8, 24hrs) treated groups at 3rd day in comparison with the control group and have been recovered to the condition of the normal group. 6. The contents of LDH in the blood serum of the white rat have been decreased in B.E.P.(8, 24hrs) treated groups at 3rd day in comparison with the control group, in particular 24hrs-B.E.P. treated group has been decreased distinctly than the normal group. 7. The contents of T-protein in the blood serum of the white rat have been distinctly increased in B.E.P. (8, 24hrs) treated groups at 3rd day in comparison with the control and normal group. As the above results, it has been proved that the execise of mice and the fatigue metabolism of rats were influenced by the light energy emitted the B.E.P., and it has been also proved that the external stimulation could be used as a preferable stimulative factor for the biological metabolism. If the clinical training and study are positively achieved, the B.E.P. would be used as curative means and preventive measures for helping human body.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.11
• /
• pp.1769-1776
• /
• 2020

As part of our current work to discover structurally and/or biologically novel compounds from Korean wild mushrooms, we isolated five ergostane-type steroids (1-5) from the fruiting bodies of Xerula furfuracea via repeated column chromatographic separations and HPLC purification. The chemical structures of the isolated steroids were shown to be (22E,24R)-24-methylcholesta-4,22-diene-3,6-dione (1), ergosta-7,22-diene-3β,5α,6β-triol (2), ergosta-7,22-diene-3β,5α,6β,9α-tetraol (3), (22E,24R)-5α,8α-epidioxyergosta-6,22-diene-3β-ol-3-O-β-D-glucopyranoside (4), and (22E,24R)-5α,8α-epidioxyergosta-6,9,22-triene-3β-ol-3-O-β-D-glucopyranoside (5)based on comparison of the data regarding their spectroscopic and physical properties with those of previous studies. Notably, this is the first report on the presence of the identified steroids (1-5) in this mushroom. We tested compounds 1-5 to determine their effects on adipogenesis and osteogenesis in the mouse mesenchymal stem cell line C3H10T1/2 and found that compounds 4 and 5 suppressed the differentiation of stem cells into adipocytes. Notably, in addition to its suppressive effect on adipogenesis, compound 5 was also shown to promote the osteogenic differentiation of stem cells. These findings demonstrate that the bioactive compounds isolated might be effective for the treatment of menopause-associated syndromes, such as osteoporosis and obesity, as the isolated compounds were shown to suppress adipogenesis and/or promote osteogenesis of stem cells.

• Proceedings of the CALSEC Conference
• /
• 2002.01a
• /
• pp.19-24
• /
• 2002

e-Korea의 과제 기업의 구조개선과 생산성제고 등 질적발전으로 이어지지 못함 경제전반의 투명성 또한 개선되지 못하고 있는 실정 Economist가 발표한 2000년 35개 국가별 불투명 지수에서 한국은 5위(73점)를 차지했으며, 특히 회계-경영분야는 가장 불투명(1위, 90점) 세계 e-Business 환경이 급속히 변화(중략)

• Microbiology and Biotechnology Letters
• /
• v.26 no.2
• /
• pp.89-95
• /
• 1998

Rhodopseudomonas sphaeroides K7 and E15-1 produced hydrogen from glucose rapidly for the first 24 hrs of culture under the anaerobic and photosynthetic conditions and then ceased the hydrogen production because of the accumulation of organic acids such as acetic acid and formic acid in the culture broth, decreasing the pH to 4.2-4.5. Only 43% and 73% of glucose in the culture were consumed even after 6 days of incubation by R. sphaeroides K7 and E15-1, respectively. The hydrogen production and glucose consumption, however, were substantially increased when the pH of the culture was adjusted to 6.8-7.0: Hydrogen production continues even after 10 days of culture and glucose was consumed completely after 2.5 and 4.5 days by R. sphaeroides K7 and E15-1, respectively, Furthermore, the bacteriochlorophyll contents in R. sphaeroides K7 and E15-1 were increased by 44 and 9 folds and the cell concentrations by 10 and 2.5 folds, respectively, after 7 days of culture. R. sphaeroides K7 and E15-1 also produced hydrogen from acetic, lactic, butyric and malic acids under the anaerobic and photosynthetic conditions even though the amounts of hydrogen produced were lower than that from glucose. The results of this experiment indicate that under the anaerobic and synthetic conditions R. sphaeroides K7 and E15-1 might use the NADH oxidation mediated by ferredoxin and hydrogenase to evolve hydrogen from glucose for the first 24 hrs and then the organic acids produced were used as electron donners for the production of hydrogen in the nitrogen-limited condition.

• Reproductive and Developmental Biology
• /
• v.30 no.1
• /
• pp.7-11
• /
• 2006

This study was conducted to examine the effects of hormone and sodium pyruvate on in vitro maturation of canine oocytes. Canine oocytes were collected from the ovaries of dogs and cultured in NCSU-37 medium with hormones and sodium pyruvate for 72 hr. Oocytes matured to the metaphase II (MII) stage were observed only from estradiol $17{\beta}\;(E_2)$, and the presence of gonadotropin did not improve the nuclear maturation. No oocytes were developed to the MII stage when $E_2$ was added to medium during the first 6 and 24 hrs of culture period. The presence of $E_2$ during the whole culture period enhanced the nuclear maturation to the MII stage (6.0%, P<0.05). High concentration of sodium pyruvate (2.5 mM) slightly enhanced the nuclear maturation to the metapahse I (HMI) stage, but not the MII stage. the result of the present study shows that the presence of $ E_2$ during the whole culture period of 72 hr enhances the maturation of canine oocytes to the M stage, but sodium pyruvate does not affect the nuclear maturation of the canine oocytes.