• 한국동물위생학회지
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• 제44권4호
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• pp.247-256
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• 2021

The recombinant lysozyme-HJL34 proteins were expressed and purified using commercial Escherichia (E.) coli expression system. Stx2e⁺ F18⁺ E. coli, Actinobacillus pleuropneumoniae (APP), Streptococcus (S.) suis, and Clostridium (C.) perfringens strains were isolated from pigs. The minimum inhibitory concentrations (MICs) of the recombinant lysozyme-HJP34 proteins were examined by means of the microtiter plate method, according to the NCCLS recommendations. The possibility of its as the alternatives to antibiotics was tested in piglets. The MICs were determined as 75 ㎍/mL, 300 ㎍/mL, 75 ㎍/mL, 35.5 ㎍/m against Stx2e⁺ F18⁺ E. coli, APP, S. suis, C. perfringens, respectively. A total of 25 piglets were divied 5 groups. The piglets in group A~C were fed with commercial feed and those in groups D, E were fed with commercial feedstuff. All piglets in groups B~E were challenged with virulent Stx2e⁺ F18⁺ E. coli, APP, S. suis strains. Groups C and D were treated with antimicrobial from 24 h after challenge. All piglets in group B died within 3 days after challenge. Among 5 piglets in groups C and D piglets, 80% survived after challenge. Among group E piglets, 60% were alive until the end of this study. Therefore, this study indicates that recombinant lysozyme-HJP34 proteins is a suitable possibility as a feed additive for reduction of diseases by bacterial pathogens in piglet feed.

• 대한미생물학회지
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• 제22권3호
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• pp.267-273
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• 1987

In the analysis of plasmid profile obtained by agarose gel electrophoresis, the strain harvoring multiple reference plasmids of known molecular weight were needed to estimate the size of unknown molecular weight plasmids. Six strains of E. coli isolated from clinical specimens carried multiple plasmids and these strains could be available as a reference plasmids harvoring strain. These E. coli strains showed 4 to 9 plasmids of various size ranging 2.5 to 94.3 megadalton (Mdal). The correlation coefficients of linear regression between the relative mobility and molecular weight were 0.99966 to 1.00000. Among them, E. coli KE327 from throat which contained 7 plasm ids as follows: 79 Mdal, 46 Mdal, 33 Mdal(pKY3027 C), 4.9Mdal, 3.8Mdal(pKY3027 E), 3.5Mdal, and 2.7 Mdal. Relative amount of the pKY3027 C was the smallest(7.09%) and that of the pKY3027 E was the largest (24.24%) among the plasmid fractions of E. coli KE327.

• Journal of Microbiology and Biotechnology
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• 제16권1호
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• pp.20-24
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• 2006

Plasmid DNA (pDNA) is a product of interest for many biopharmaceutical companies and research laboratories, because of increase in the number of gene therapy protocols that use nonviral vectors. This work was undertaken to study the effect of antibiotic and dissolved oxygen concentration (DOC) on the production of a ColE 1-type plasmid (pVAX1-LacZ) hosted in Escherichia coli $DH5\alpha$ and cultured in a batch fermentor with 0.751 of Terrific Broth. A decrease in the DOC from $60\%\;to\;5\%$ was shown to increase the specific pDNA concentration approximately 1.5-fold, due to the downregulation of growth. Additionally, this increase in the pDNA concentration led to a 2.2-fold increase in the purity of cell lysates obtained after cell lysis. However, the use of higher DOC led to 2.8-fold higher volumetric productivity as a consequence of a faster growth rate, reducing the fermentation time from 24 to 8 h. Interestingly, the specific pDNA concentration, and pDNA productivity and purity were always higher $(10-15\%)$ in the absence of antibiotic. Overall, the data indicate that nonselective conditions can be used without compromising yield, productivity, and purity of pDNA.

• Transactions on Electrical and Electronic Materials
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• 제13권5호
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• pp.221-224
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• 2012

The present paper reports the comparative optical behavior of a pure nematic mixture E-24 and its anthraquinone dye doped sample. The variation in the ordinary and extraordinary refractive index ($n_o$, $n_e$) of the pure and dye doped samples with temperature has been discussed and it has been found that doped sample have a less refractive index compared to the pure sample. The variation in the order parameter for pure and doped samples with temperature has been discussed and it has been seen that the doped sample has a higher order parameter; the possible reasons have been discussed. The variations in birefringence and optical transmittance have also been presented here.

• 한국지형학회지
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• 제24권4호
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• pp.43-55
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• 2017

This study tries to examine papers on coastal terrace in the East Coast of South Korea and to summarize formative age and elevation of the terrace. Spatial and temporal variations of uplift rate in the Coast based on absolute age published are also reviewed. The terrace in the middle part in the Coast from Goseong to Samcheok distributes in an elevation of 10-20 m and its formative age is MIS 5a. The terraces during MIS 5e and 7 develop on an elevation of 20-35 m and 60-80 m, respectively. The mid-southern part in the Coast from Uljin to Yeongil Bay has the terraces with elevations of 10-25 m and 25-45 m and their ages are MIS 5a or 5c and 5e, respectively. The terraces with elevations of 10-25 m and 30-45 m correspond to MIS 5a and 5e, respectively, in the southern part in the Coast from Homigot to Busan. Assuming that elevation of sea level during the formation of each terrace is the same as in the present time, uplift rates in the Coast range from 0.05 to 1.36 m/ky with an average of approximately 0.33 m/ky. The highest and lowest rates since MIS 5 are found in the Gyeongju (approximately 0.39 m/ky) and Pohang (approximately 0.19 m/ky) areas. With a consideration of elevation of sea level at that time, however, the middle, midsouthern and southern parts in the Coast show uplift rates of 0.16-0.28 m/ky, 0.20-0.36 m/ky and 0.24- 0.36 m/ky since MIS 5, respectively, suggesting that the southern part in the Coast has experienced relatively higher uplift rate.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1987년도 정기총회 및 창립40주년기념 학술대회 학회본부
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• pp.504-507
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• 1987

Interpenerating Polymer Networks (IPNs) are unique type of polymer blend, synthesized by swelling a crossed polymer (Epoxy) with second polymer (Silicon) and also we adopted Urethane as the second polymer. The relationship between dielectric and mechanical properties of high temperature curing IPNs(E/S, E/U) are investigated. The ratios of weight that we formed we re two kind of thing, one (E/S) about 1[wt%]. 3[wt%], 5[wt%], 7[wt%], 10[wt%], and the other (E/U) about 5[wt%], 15[wt%], 25[wt%]. It was heat-cured for 24hours at $100^{\circ}C$ 48 hours at $10^{\circ}C$, 5 hours at $130^{\circ}C$, 15hours at $130^{\circ}C$ in E/S and also for 5 hours at $130^{\circ}C$ in E/U. From the viewpoint of dielectric and mechanical properties, the optimum condition is obtained from the sample cured for 5hours at $130^{\circ}C$ for 1[wt%] in the E/S, and also obtained from the sample cure d for 5 hours at $130^{\circ}C$ in E/U.

• 대한정형외과스포츠의학회지
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• 제10권2호
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• pp.86-93
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• 2011

목적: 가장 뛰어난 수행 능력을 가진 국가대표 엘리트 태권도 선수의 국제 경기 중의 손상에 대하여 분석하고자 하였다. 대상 및 방법: 2011 경주 세계 선수권 대회에 참가한 146개국 국가대표 950명이 전체 1,063 경기 중에 손상을 받은 52명을 대상으로 하였으며 성별, 연령별, 체급별 경기 손상률과 손상 부위별 손상 발생률과 손상 유형, 그리고 손상 양상에 따른 손상 부위 등에 대하여 분석하였다. 결과: 총 950명, 전체 1,063 경기에서 52명이 손상을 입어 5.47%가 경기 손상을 받았고, 1,000 경기 노출당(per 1,000 athlete-exposures, /1,000 A-E) 손상률은 24.46/1,000 A-E이었다. 여자의 손상률이 남자보다 높았지만 통계학적으로 유의한 차이는 없었다(p=0.512). 연령별로는 30세 이상에서 손상률이 참가자 100명당 17.39%로 가장 높았으며, 체급별로는 남자에서 87kg 이상에서 손상률이 9.23%로 가장 높았고, 여자에서 67~73kg 체급의 손상률이 18.18%로 가장 높았으나 통계학적 유의성은 없었다(p=0.838). 손상 부위는 전체적으로는 하지의 손상률이 13.17/1,000 A-E로 상지나 두경부, 체간 부위보다 높았지만, 손상 부위별로는 수부 손상의 손상률이 5.17/1,000 A-E로 가장 높았고 슬관절 부위가 4.70/1,000 A-E로 다음이었다(p=0.714). 손상 유형으로는 관절 염좌의 손상률이 10.81/1,000 A-E로 가장 높았으며, 다음 순위로 남자에서는 골절의 손상률이 5.40/1,000 A-E로 높았으나 여자에서는 좌상이 9.63/1,000 A-E로 높았다(p=0.033). 중증 손상인 골절은 8예 중 4예에서 수부에서 발생하였다. 결론: 2011 경주 세계 선수권 대회에 참가한 엘리트 태권도 선수의 국제 경기 중 손상률은 5.47%, 24.46/1,000 A-E였다. 전체적으로는 슬관절과 족관절의 염좌가 가장 많은 손상을 차지하였지만 중증 손상인 골절은 수부에서 가장 많이 발생하였다.

• Applied Science and Convergence Technology
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• 제24권5호
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• pp.162-166
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• 2015

Linear ion beams have been introduced for the ion beam treatments of flexible substrates in roll-to-roll web coating systems. Anode layer linear ion sources (300 mm width) were used to make the linear ion beams. Oxygen ion beams having an ion energy from 200 eV to 800 eV used for the adhesion improvement of Cu thin films on PET substrates. The Cu thin films deposited by a conventional magnetron sputtering on the oxygen ion beam treated PET substrates showed Class 5 adhesion defined by ASTM D3359-97 (tape test). Argon ion beams with 1~3 keV used for the ion beam sputtering deposition process, which aims to control the initial layer before the magnetron sputtering deposition. When the discharge power of the linear ion source is 1.2 kW, static deposition rate of Cu and Ni were 7.4 and $3.5{\AA}/sec$, respectively.

• 대한의생명과학회지
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• 제29권4호
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• pp.287-295
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• 2023

Amifostine was developed to protect cells, but it is known to induce cytotoxicity and apoptosis, and the exact mechanism is unknown. In this study, we investigated how the DNA mismatch repair (MMR) system interacts with p53 to prevent apoptosis, cell cycle arrest, and cytoprotective effects induced by amifostine. HCT116 colon cancer cells sublines HCT116/p53+,HCT116/p53+, HCT116/p53-, HCT116/E6 and HCT116+ch3/E6 cells were used for evaluation. Amifostine induced G1 arrest and increased toxicity two-fold in p53- cells regardless of MMR expression. Both G1 cell cycle arrest and induction of p53 protein peaked at 24 h after the start of amifostine exposure. Both G1 cell cycle arrest and induction of p53 protein peaked at 24 h after the start of amifostine exposure. Amifostine induced the expression of p21 protein in both p53+ and p53- cells. As for apoptosis, compared to p53- cells, p53+ cells showed 3.5~4.2 times resistance to amifostine-induced apoptosis. HCT116+E6 with both p53 and MMR loss showed maximum apoptosis at 48 h, and HCT116+ch3/E6HCT116+ch3/E6 with p53 loss showed maximum apoptosis at 24 h. As a result, it was confirmed through in vitro experiments that amifostine-induced G1 cell cycle arrest and apoptosis are mediated through a pathway dependent on MMR and p53 protein.

• 한국항행학회논문지
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• 제24권2호
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• pp.73-84
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• 2020

자동차 대수의 증가로 인한 지상 교통의 혼잡을 극복하기 위해 많은 회사들이 새로운 방식의 운송 수단인 미래형 개인항공기(PAV)를 제안하였다. 미래형 개인항공기 중에서도 전기를 동력으로 사용하고 수직 이착륙이 가능한 전기수직이착륙(eVTOL)항공기가 주목을 받고 있으며 그러한 항공기들의 형상은 멀티콥터형에서 틸트 덕티드팬까지 다양하다. 본 연구에서는 eVTOL 유형별 장단점 등 특성을 분석하였다. 틸트날개형, 복합형, 멀티콥터형의 대표적인 eVTOL PAV인 바하나, 오로라, 볼로콥터에 대해 구성성분 합계 방식을 사용하여 유해항력을 구하였으며, 항공기 설계 및 공력 해석 프로그램인 OpenVSP와 XFLR5 프로그램을 사용하여 표면적과 유도항력을 구하였다. eVTOL PAV에 사용되는 배터리는 테슬라 2170 배터리로 가정하고 항속거리를 계산하였다. 또한 각 eVTOL에 대해 이착륙 및 순항을 포함한 임무형상별로 에너지소모 및 최대 비행시간을 계산하여 비교하였다.