• Natural Product Sciences
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• v.2 no.1
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• pp.9-13
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• 1996

Three new withanolides, designated as withatatulins B, C, and D, were isolated from the fresh flowers of Datura tatula Linn. Detailed spectral analysis of these compounds permitted advancement of their structures respectively, as $5{\beta},6{\beta}-epoxy-12{\beta}$,21-dihydroxy-1-oxo-witha-2,24-dienolide (2), $6{\beta},12{\beta},21-trihydroxy-1-oxowitha$ 2, 4,24-trienolide (3) and $5{\beta},6{\beta},12{\beta}$,21-tetrahydroxy-1-oxo-witha-2,24-dienolide (4a). Withanolides with oxygen functions both at 12 and 21-positions are rare and first reported from Datura species.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.6
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• pp.956-963
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• 1995

Twenty esters, 14 alcohols, 5 aldehydes, 5 ketones, 5 lactones, 2 S-containing compounds and 1 hydrocarbon are identified by GC-MS from volatile compounds in a glucose solution containing Yeat-Carbon-Base medium fermented for 64 hrs by Saccharomyces bayanus at pH 3.5, $25^{\circ}C$, 400rpm and 35L/h of aeration for 24hrs. Under the different conditions of conservation(1~4), ethyl 2-hydroxy-4-metjhylpentanoate, ethyl succinate, nonanol and phenylacetaldehyde are produced during conservation of fermented solution. 17 esters increased during conservation at $13^{\circ}C$ for 12 weeks and the increase of ethyl 9-hexadecenoate is important among 13 esters increased during conservation at $35^{\circ}C$ for 24hrs. During conservation, aldehydes increased at $35^{\circ}C$, but decreased at $13^{\circ}C$ and the great increase of isobutanal, benzaldehyde and phenylacetaldehyde is observed at $35^{\circ}C$. Alcoho and lactones increased but ketones decreased during conservation.

• Microbiology and Biotechnology Letters
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• v.7 no.1
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• pp.9-14
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• 1979

The hemicellulose fraction of ryegrass straw was extracted with NaOH and used for production of glucose isomerase by Streptomyces flavogriseus. Up to 25％ crude hemicellulose (20％ as pentosan) could he obtained by treating straw with 1 to 24％ NaOH. The yield of hemicellulose was increased proportionately with increasing NaOH concentration up to 4％, but the rate of increase was slowed thereafter. The optimum condition for hemicellulose extraction from ryegrass straw was to treat straw with 4％ NaOH for 3hrs at 9$0^{\circ}C$ or 24hrs at 3$0^{\circ}C$. Highest level of glucose isomerase activity (3.04 units/ml culture) was obtained when the organism was grown for 2 days at 3$0^{\circ}C$ on 2％ straw hemicellulose. The organism also produced a good quantity of glucose isomerase on xylan, xylose or H$_2$SO$_4$-hydrolysate of straw. The hemicellulose-extracted straw residue could be used as animal feed, because the residue had 75％ higher digestibility and 20％ better feed efficiency for weanling meadow voles than the untreated straw.

• The Journal of Korean Physical Therapy
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• v.24 no.2
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• pp.127-133
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• 2012

Purpose: This study aims to examine the power changes in eletrocenphalogram (EEG) detected from the tibialis anterior muscle, during repetitive contraction exercise in normal female adults. Methods: The subjects of this study were 24 normal adult females, with no musculoskeletal or nervous system disorders. The 24 female subjects were divided into two groups: 12 subjects comprised a voluntary stimulation training group, and the other 12 subjects comprised an electrical stimulation training group. A total of thirty contractions were made repetitively by each woman, with maximal voluntary contraction exercise for six seconds, and a resting time of three seconds. During the experiment, their EEG was measured at eight positions. The eight positions were Fpz, Fz, Cz, CPz, C3, C4, P3, and P4, in accordance with the international 10~20 system. Results: The relative alpha power and beta power showed no statistically significant differences between the two groups. But the relative gamma power of the CPz, C3, C4, P3, and P4 areas showed statistically significant differences between the two groups (p<0.05). The relative theta power of the C4 area showed statistically significant differences between the two groups (p<0.05). Conclusion: Our findings show that tibialis anterior muscle contraction by electrical stimulation and by voluntary repeated contraction differentially affected brain activation. In particular, the CPz, C3, C4, P3 positions of relative gamma power showed brain activation in voluntary contraction. The C4 position of relative theta power showed different brain activation between the two groups.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.5
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• pp.738-744
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• 2008

In most developing countries consumers purchase retail cuts from hot carcasses and prepare traditional meat products as per their convenience and requirements. In this study, effects of different post mortem handling practices on quality of meat curry from culled sheep meat have been studied. After slaughter, leg cuts were subjected to nine commonly prevalent handling conditions in India viz. deboning (boning out) and cooking within 2-3 h (1), deboning immediately and cooking after 5-6 h (2), deboning after 5-6 h and cooking (3), deboning immediately, storage at $4^{\circ}C$ for 24 h and cooking (4), chilling for 24 h at $4^{\circ}C$, deboning and cooking (5), deboning after 5-6 h, storage for 24 h at $4^{\circ}C$, and cooking (6), deboning after 5-6 h, storage for 48 h at 4??C and cooking (7), deboning after 5-6 h, freezing and cooking (8), deboning after 5-6 h, storage for 24 h at $4^{\circ}C$, freezing and cooking (9). Significant differences were observed in pH, water-holding capacity, cooking loss and shear force values. Sensory scores were significantly higher in conditions (1), (5) and (9), and significantly lower in conditions (4) and (6). From the results, it was concluded that, to have the best quality product, meat should be cooked either immediately after slaughter or should be deboned just before cooking. Storage of deboned meat at refrigerated temperature must be avoided.

• Journal of Chest Surgery
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• v.21 no.3
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• pp.425-446
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• 1988

After 24 hours of preservation under 15 mmHg perfusion pressure the recovery rates of isolated canine hearts were determined. Preservation was performed in a cold room maintained at 4*C with 4 different types of perfusates bubbled with a mixture of 95% 0y and 5% CO~ using a modified perfusion unit designed in our institute. The perfusates used were as follows; Group 1: Krebs-Henseleit solution, Group 2: Krebs solution added by albumin and PGE1. Group 3: Modified Wicomb*s solution, Group 4: Modified Collin*s solution. The extent of myocardial recovery was evaluated using a modified isolated carmine perfusion model by measuring heart rate, systolic arterial pressure, left atrial pressure[LAP] and cardiac output. In addition to the above hemodynamic parameters, biochemical and enzymatic assays from perfusates and electron microscopic changes of the myocardium were also studied. The results were as follows; 1] The heart recovery rates were 41.6%, 53.4% and 108.9% in groups 1, 2 and 3, respectively, and group 3 elicited the best result[p< 0.001]. The heart beat was never recovered in group 4. 2] Recovered systolic arterial pressures[mmHg] were 63.3% in group 1, 94.9% in group 2 and 94.3% in group 3. 3] LAPs[mmHg] were 20 in group 1, 13.5 in group 2 and 11.2 in group 3, which suggested that the best myocardial preservation was elicited in group 3[p< 0.05]. 4] Cardiac output, the sum of aortic stroke volume and coronary leakage, were 69.1% in group 2, and 90.7% in group 3, but these were not statistically significant[p=0.24]. No aortic stroke output was measured in group 1 and 4. 5] The degree of myocardial edema increase was 17.5` in group 1, 24.6% in group 2, 20.9% in group 3 and 55.3% in group 4. But there were no statistical differences in each group[p= 0.08]. 6] CPK-MB[U/L] levels were increased 750% and 332%[p< 0.05], glucose levels[mg/dl] 60.5% and 78.2% and SGOT[U/L] levels 523% and 333%, in groups 2 and 3, respectively. Biochemical and enzymatic assays could not be performed in group 1 and group 4, because of poor recovery of heart beat. 7] Electron microscopic findings in the myocardium of most groups revealed slight to moderate muscle cell and mitochondrial edema. But all these findings were within the limits of reversible change. From these above results, it is suggested that modified Wicomb*s solution seems to be the most useful physiologic salt solution for preservation of the heart. We propose that after further study and improvement, our portable continuous hypothermic perfusion system will contribute to the development of a better preservation method for donor hearts for human heart transplantation.

• Microbiology and Biotechnology Letters
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• v.17 no.4
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• pp.313-320
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• 1989

Rhodotorula glutinis K-24 was found to produce internal, cell wall bound, and external invertase. Internal invertase was purified by column chromatographies on DEAE-Sephadex A-50, Sp-sephadex C-50, gel filtration on Sephadex G-200 and isoelectric focusing. Cell wall bound invertase was partially purified by the following procedures; column chromatography on DEAE-Sephadex A-50 and gel filtration on Sephadex G-100. Optimum pH and temperature for enzymatic activities of internal and cell wall bound invertase were pH 3.0 and 6$0^{\circ}C$, respectively. Both enzymes were inhibited by HgC1$_2$, AgNO$_3$, MnSO$_4$, and sodium dodecylsulfate. The molecular weights of internal and cell wall bound invertases were estimated to be 310,000 and 61,000, respectively. Other physicochemical properties of the both enzymes were similar.

• Korean Journal of Plant Resources
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• v.27 no.4
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• pp.365-370
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• 2014

This study was conducted to determine the effects of seed storage methods ($-20^{\circ}C$, stratification, $2^{\circ}C$ dry, $2^{\circ}C$ wet and room temperature) and $GA_3$ application (control, $dH_2O$, 10, 100, 1000 ppm) on seed germination and seedling growth of S. lyratum. As a result seed germination rate of S. lyratum was the highest at 91% when seeds were stored at room temperature and then soaked for 24 hours in $GA_3$ 10 ppm. And seedlings of S. lyratum showed the best quality when seed were stored at $2^{\circ}C$ dry and then soaked for 24 hours in $GA_3$ 1000 ppm, with the growth characteristics of plant height (47 mm), number of leaves (8), leaf width (12 mm), leaf length (19 mm), fresh weight (aerial/root part; 471/476 mg), dry weight (aerial/root; 106/41 mg) and seedling quality indices (106). Therefore, S. lyratum seed were stored at $2^{\circ}C$ dry, and then soaked for 24 hours in $GA_3$ 1000 ppm, seed germination rate was more than 80% and production of superior quality container seedlings.

• Microbiology and Biotechnology Letters
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• v.18 no.4
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• pp.368-375
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• 1990

Rhodoto& ghtbth~ K-24 was found to produce external invertase in addition to internal and cell wall bound invertase. External invertase was purified to an electrophoretically homogeneous state and partitally characterized and was compared with internal and cell wall bound invertase of which procedures for purification and characterization were reported previously. The enzyme was purified by ethanol precipitation, column chromatographies on DEAE-Sephadex A-50 and SP-Sephadex C-50, and gel filtration on Sephadex G-100. The molecular weight and subunit molecular weight of external invertasGwere estimated to be 220,000 and 100,000, respectively. The isoelectric point of the enzyme was about pH 6.0. The optimum pH and temperature for enzyme activity were pH 4.0 and $60^{\circ}C$, respectively. The enzyme remained stable at the wide range, from pH 3.0 to 11.0 and stable up to $40^{\circ}C$, but was inactivated at temperatures above that. $HgC_12, AgN0_3, MnS0_4$, SDS and p-CMB inhibited the enzyme activity. The $K_m$ value of the enzyme for sucrose was $1.0\times 10^{-2}$M. From these results, the three isozymes from Rh. glutinis K-24 seem to have the similar enzymatic properties, but to differ in molecular and subunit weights.

• Journal of the Korean Wood Science and Technology
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• v.32 no.4
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• pp.52-57
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• 2004

Seven compounds were isolated from stationary cultured mycelia of Inonotus obliquus, of which several of them showed certain antitumor activities. Seven compounds were identified as lanosterol(1), inotodiol(2), trametenolic acid(3), 3β,22,25-trihydroxy-lanosta-8-ene(6), 3β,22-dihydroxy-lanosta-8,24-diene(A), 3β-hydroxy- lanosta-8,24-dien-21-al(B) and methyl trametenolate(C), respectively. The precursor compound of sclerotium obtained from shaking-cultured and stationary-cultured mycelia of Inonotus obliquus is lanosterol. Ergosterol and ergosterol peroxide were obtained by shaking-culture, and the substituted compounds of C-21 and C-22 of lanosterol were obtained by stationary culture.