• 제목/요약/키워드: %24C_4%24%24A_3%24S

검색결과 1,853건 처리시간 0.039초

반 정량 식품빈도 조사법 (SQFFQ)과 24시간 회상법을 이용한 영양평가 Software 개발 (Software for Nutritional Assessment Using a Semi-Quantitative Food Frequency Questionnaire and the 24-hour Recall Method)

  • 이상아;이경신;김형숙;이해정;최혜미
    • 대한지역사회영양학회지
    • /
    • 제7권4호
    • /
    • pp.548-558
    • /
    • 2002
  • The purpose of this study was to develop a computer software program for nutritional assessment using a Semi-Quantitative Food Frequency Questionnaire (SQFFQs) and the 24-hour Recall Method. The software for the SQFFQ was divided into input, output, and database. For dietary analyses, recipe and food databases were used. The recipe database included 25 items and the food database was divided into 18 food groups. The food database was composed of 19 general nutrient items, 33 fatty acids, and 18 amino acids. The software developed in this study can be summarized as follows: 1) input items related to the individual s ages information, lifestyle, biological values, and dietary habits; 2) individualized data in percent of the Korean RDA, the energy ratios of carbohydrates, proteins and fats, the ratio of animal to plant source intakes, and the distribution of food group intakes; 3) Statistical data on the individual's information, lifestyle, biological values, and dietary intakes including the frequency of intake of cooked foods, the amounts of food, and the number of food groups, and nutrients. In the 24-hour Recall Method, the input and output consisted of the individual s information and cooked dish intakes. The individual s report included the amounts of nutrient intake according to number of meal and days, in comparison to the Korean RDA, the energy ratio for carbohydrates, proteins and fats, the ratio of animal to plant source intakes, and the distribution of food group intakes. The statistical report presented the number of food groups and foods, and the nutrient intakes. To evaluate the validity of the SQFFQ, the Spearman Rank Order Correlation and kappa values were used. As a result, correlation coefficients comparing the 24-hour Recall Method appeared to be more than 0.5, except for vitamin $B_1, B_2$, niacin, and vitamin E. The kappa values for energy and carbohydrate intakes were both 0.7, and protein, fat, vitamin C, folate, Ca, and iron intakes ranged from 0.3 to 0.7.

A New 5α, 8α-Epidioxy Sterol from the Marine Sponge Plakortis simplex

  • Oh, Jung-Soon;Kim, Myoung-Ha;Song, Ah-Rin;Rho, Jung-Rae
    • 한국자기공명학회논문지
    • /
    • 제14권1호
    • /
    • pp.1-8
    • /
    • 2010
  • Four 5,8-epidioxy sterols were isolated from the marine sponge Plakortis simplex. Their structures were completely determined by an extensive NMR analysis and comparison with NMR data of similar compounds for absolute stereochemistry of the side chain. The compounds were assigned as 5,8-epidioxy- (24S)-ethylcholesta-6,22(E),25-trien-3-ol(1), 5,8-epidioxy-(24S)-methylcholesta- 6,22(E)-dien-3-ol(2), 5,8-epidioxycholesta-6,22(E)-dien-3-ol(3) and 5,8- epidioxycholesta-6-en-3-ol (4).

Simultaneous Determination of C22-C26 Very Long - Chain Fatty Acids Following tert-Butyldimethylsilyl Derivatization by Stable Isotope GC- MS for the Screening of Adrenoleucodystrophy

  • Yoon, Hye-Ran
    • Biomolecules & Therapeutics
    • /
    • 제15권4호
    • /
    • pp.266-272
    • /
    • 2007
  • A rapid analytical method was developed to quantify very long-chain fatty acids (VLCFAs, C22:0, C24:0, C26:0) in human plasma with good sensitivity and specificity using tert-butyldimethylsilyl (TBDMS) derivatization and stable isotope GC-MS selective ion monitoring (GC-MS/SIM). Two-hundred and fifty ${\mu}L$ of plasma was fortified with deuterated stable isotope internal standards (d3-C22:0, d3-C24:0, d3-C26:0) and standard mixtures of chloroform and methanol, and then extracted with hexane and acetonitrile. To upper layer of liquid-liquid-extraction, N-(t-Butyldimethylsilyl)-N-methyltrifluoroacetamide was added and then heated to $60^{\circ}C$ for 30 min to produce the TBDMS derivatives. Derivatives of VLCFAs were analyzed by GC-MS/SIM. Calibration curves showed a linear relationship for the target compounds in the concentration range of $10^{-4}{\sim}2{\times}10^3\;{\mu}g/mL$ with the correlation coefficient ranging from 0.996 to 0.999. The limit of quantification for the plasma was $10^{-4}{\sim}2{\times}10^{-4}\;{\mu}g/mL$ (S/N=3). When applied to the plasma specimens of patients with peroxisomal disorder, X-linked adrenoleucodystropy (ALD, Mckusick 202370), the method clearly differentiated normal subjects from ALD patients. The C24:0/C22:0 and C26:0/C22:0 ratios were significantly elevated in the plasma of patients with X-linked ALD compared to normal subjects. The new developed method might be useful for a rapid and sensitive diagnosis of X-linked ALD and other peroxisomal disorders.

미생물을 이용한 트립신 과대 생산 연구 - Streptomyces용 숙주-벡터계를 이용한 트립신 유전자의 대량발현 최적화 - (Overproduction of Bacterial Trypsin in Streptomyces - Optimization for Streptomyces griseus Trypsin Production by Recombinant Streptomyces)

  • 김종희;홍순광
    • 한국미생물·생명공학회지
    • /
    • 제36권1호
    • /
    • pp.28-33
    • /
    • 2008
  • Streptomyces griseus trypsin (SGT)을 코드하는 sprT 유전자와 그 하류에 존재하는 두 개의 조절 유전자 rsgtR1 및 sgtR2를 동시에 갖고 있는 재조합 벡터 pWHM3-TR1R2를 S. lividans TK24 및 S. griseus IFO 13350에 도입하여, 트립신의 생산성을 더욱 증대시킬 수 있는 배지를 조사하였다. S. lividans TK24/pWHM3-TR1R2의 경우 배양 5일을 기준으로 R2YE에서 가장 높은 생산성(0.74 unit/mL)을 나타냈고, C5/L. (0.66 unit/mL), Livid (0.08 unit/mL), NDSK(0.06 unit/mL) 순으로 나타났다 S. griseus IFO 13350/pWHM3-TR1R2의 경우에는 전반적으로 배양 7일에 트립신 활성이 가장 높았으며, C5/L (1.518 unit/mL), R2YE(1.284 unit/mL), NDSK (0.932 unit/mL), Livid (0.295 unit/mL) 순으로 나타났다. S. griseus IFO 13350/pWHM3-TR1R2를 C5/L 배지에서 7일간 배양한 배양액으로부터 $25%{\sim}60%$ ammonium sulfate 침전, CM-sepharose 및 Sp-sepharose column chromatography를 통하여 트립신을 고순도로 정제할 수 있었다. 최종 purification fold는 6.5배, 순수 정제된 트립신의 specific activity는 69,252 unit/mg, 회수율은 1.4%이었다.

Cloning and Characterization of a Heterologous Gene Stimulating Antibiotic Production in Streptomyces lividans TK-24

  • Kwon, Hyung-Jin;Lee, Seung-Soo;Hong, Soon-Kwang;Park, Uhn-Mee;Suh, Joo-Won
    • Journal of Microbiology
    • /
    • 제37권2호
    • /
    • pp.102-110
    • /
    • 1999
  • Genetic determinant for the secondary metabolism was studied in heterologous expression in Streptomyces lividans TK-24 using Streptomyces griseus ATCC 10137 as a donor strain. Chromosomal DNA of S. griseus was ligated into the high-copy number Streptomyces shuttle plasmid, pWHM3, and introduced into S. lividans TK-24. A plasmid clone with 4.3-kb BamHI DNA of S. griseus (pMJJ201) was isolated by detecting for stimulatory effect on actinorhodin production by visual inspection. The 4.3-kb BamHI DNA was cloned into pWHM3 under the control of the strong constitutive ermEp promoter in both directions (pMJJ202); ermEp promoter-mediated transcription for coding sequence reading right to left: pMJJ203; ermEp promoter-mediated transcription for coding sequence reading left to right) and reintroduced into S. lividans TK-24. The production of actinorhodin was markedly stimulated due to introduction of pMJJ202 on regeneration agar. The introduction of pMJJ202 also stimulated production of actinorhodin and undecylproidigiosin in submerged culture employing the actinorhodin production medium. Introduction of pMJJ203 resulted in a marked decrease of production of the two pigments. Nucleotide sequence analysis of the 4.3-kb region revealed three coding sequences: two coding sequences reading left to right, ORF1 and ORF2, one coding sequence reading right to left, ORF3. Therefore, it was suggested that the ORF3 product was responsible for the stimulation of antibiotic production. The C-terminal region of ORF3 product showed a local alignment with Myb-related transcriptional factors, which implicated that the ORF3 product might be a novel DNA-binding protein related to the regulation of secondary metabolism in Streptomyces.

  • PDF

전통 발효 식품에서 분리한 유산균을 이용한 yogurt 발효특성 (Fermentative characteristics of yogurt using lactic acid bacteria isolated from Korean traditional fermented food)

  • 박나영;이신호
    • 한국식품저장유통학회지
    • /
    • 제24권5호
    • /
    • pp.707-713
    • /
    • 2017
  • 전통식품에서 요구르트 제조 가능한 유산균을 선발하기 위하여 김치, 식혜 막걸리에서 분리한 유산균 50균주 중 커드 형성 능력이 있는 16균주를 선발, L. monocytogenes, S. aureus, B. cereus, E. coli, S. entritidis, V. vulnificus에 대한 항균활성과 인공 위액과 담즙액에 대한 내성이 우수한 4 균주를 선발하였다. 4균주의 환원탈지유에서 배양특성비교. 항균활성과 인공 위액과 담즙에서의 내성 등을 고려하여 KM24(구균)와 KM32(간균)을 최종 선발하여 동정한 결과 각각 Pediococcus pentosaceus, Lactobacillus plantarum로 동정되었다. 요구르트 제조 중 유산균수는 KM24와 KM32균주 혼합 접종구가 가장 높았으며, 배양 48시간째 9.66 log CFU/mL을 나타내었다. pH는 배양 48시간째 각각 4.25(혼합 접종구), 3.77(대조구)을 나타내었다. 산도의 변화는 pH 변화와 유사하여, 배양 72 시간 후 KM32 단독 접종구를 제외하고 0.88-1.24%의 범위를 나타내었다. 혼합 접종구와 대조구의 총 폴리페놀함량은 각각 38.45와 $38.86{\mu}g/mL$이었다. DPPH 라디칼 소거능은 KM24 접종구가 가장 높은 52.54%로, 혼합 접종구(48.57%)와 대조구(48.22%)에 비해 항산화활성이 우수하였다. 혼합 접종구의 경우 $4^{\circ}C$에서 10일간 저장 중 가장 높은 유산균수를 유지하였으며, 저장 10일째 9.28 log CFU/mL이었다. pH는 KM32 접종구를 제외하고 저장기간 동안 저장 초기와 유사하였으며, 산도는 저장 5일까지는 다소 증가하다가 그 이후 감소하였다. 혼합 접종구는 대조구에 비해 유산균수는 높았으나 pH는 높고, 산도는 낮은 경향을 나타내었다. 이상의 결과를 미루어 보아 분리균주인 KM24와 KM32은 요구르트 제조 시 복합 starter로 사용이 가능할 것으로 판단되었다.

인삼의 효과에 관한 세포생리학적 연구 -제 I 편 Saccharomyces의 분열에 미치는 영향- (A Cellular Physiological Study on the Effects of Korean Ginseng -Part I. Effects on the Division of Saccharomyces-)

  • 정너팔
    • The Korean Journal of Physiology
    • /
    • 제3권1호
    • /
    • pp.45-49
    • /
    • 1969
  • The effects of water extracts and powder of Korean ginseng on the division of Saccharomyces cerevisiae were studied. 1. The addition of several doses of water extracts and powder of ginseng to the yeast medium of Moyer and Coghill showed various promoted division of Saccharomyces. 2. The optimal dose of ginseng on tile division of Saccharomyces (0.08% dry ginseng medium solution per $10\;cells/mm^3$) could be recognized. 3. On the culture for 24 hours at $18^{\circ}C$, the cell number of control group was $13.25{\times}10^3\;cells/mm^3$ and that of the optimal dose group of water extracts of ginseng was $23.20{\times}10^3\;cells/mm^3$. On the culture, for 24 hours at $25^{\circ}C$, the cell number of control group was $16.85{\times}10^3\;cells/mm^3$ and that of the optimal dose group was $30.20{\times}10^3\;cells/mm^3$. The increasing rate of cell divison by the ginseng was about twice than that of control group. The optimal dose treatment of ginseng at $18^{\circ}C$ was more effective than control group at $25^{\circ}C$. 4. On the culture for 24 hours at $18^{\circ}C$, the increasing rate of water extracts of ginseng was 75.1%, and the rate of ginseng powder was 7.6%. On the culture for 24 hours at $25^{\circ}C$, the rate of water extracts of ginseng was 79.8%, and the rate of ginseng powder was 57.2%. Therefore water extracts of ginseng was more effective than ginseng powder of same dry weight, and the promoted effect of ginseng powder at $25^{\circ}C$ was more effective than at $18^{\circ}C$.

  • PDF

찹쌀첨가량에 따른 백편의 조직감 특성의 변화 (Change of Texture of Back-pyun as affected by glutinous-rice)

  • 이윤경;이효기
    • 한국식품조리과학회지
    • /
    • 제2권2호
    • /
    • pp.43-54
    • /
    • 1986
  • 백편을 만들때 멥쌀가루 100에 찹쌀가루를 총중량의 0%, 5%, 10%, 15%, 20%, 25%, 30% 첨가하고 저장시간 0시간, 24시간, 48시간, 72시간 저장한 후($4^{\circ}C$ 에서) reheating한 백편의 기호성과 조직감(Textur) 에 어떠한 영향을 끼치는 가를 관능검사와 기계적 검사에 의해 측정한 결과를 다음과 같이 요약할 수 있다. 1. 관능검사 결과 백편의 Color는 찹쌀의 첨가량이 증가할수륵 누런색이 짙어졌으며, 24,48,72시간 저장 한 후 reheating한 백편도 누런색이 짙어지는 경향을 나타냈다. Coarseness는 찹쌀의 첨가량이 증가할수록 증가하는 경향이었으나 저장전의 백편은 유의적인 경향을 나타내지 않았다. 찹쌀의 첨가량이 증가할수륵 Softness, Dryness, Crumblyness는 감소경향을 나타냈으며 24,48,72시간 저장한 후 reheating한 백편도 유의적인 감소청향을 나타냈다. 외관의 기호도는 찹쌀의 첨가율이 5%인 백편을 가장 좋아했고, 조직감 및 종합적인 기호도는 찹쌀의 첨가율이 $10{\sim}20%$인 백편을 좋아했다. 2. Rheometer에 의한 백편의 Texture는 찹쌀의 첨가량이 증가할수록 Compression force, Gumminess, Chewiness가 증가하는 경향을 나타냈다. 24,48,72시간 저장한 후 reheating한 백편은 저장전보다 점점 감소경향을 나타했다가 72시간 저장한 후 reheating하면 다시 증가경향을 나타했다. Work ratio 및 Recovered height는 찹쌀의 첨가량과 저장근간의 변화에 크게 영향을 미치지 않았다. Reheating하지 않고 0,24,48,72시간 저장한 백편의 Compression force는 찹쌀의 첨가량이 증가할수륵 증가경향이 켰고 저장시간이 경과함에 따라 Compression force가 증가하였는데 특히 24시간 저장하는 동안 급격히 증가하였다. 3. 찹쌀 첨가량이 증가할수록 수분함량은 증가하였고, 저장시간이 경과함에 따라 수분함량은 감소경향을 나타냈으나 24시간 저장한 후 reheating한 백편만이 수분함량의 증가경향을 나타했다. 4. 백편의 Softness는 Compression force 및 Work ratio와 유의적인 상관관계를 나타내었으며, Dryness와 Crumblyness는Work ratio와 유의적인 상관관계를 나타내고 있어 백편의 조직감은 Compression force 와 Work ratio로 대치할 수 있을 것이라고 사료된다. 수분함량은 기계적 검사보다 관능검사와 더욱 높은 상관관계를 나타냈다.

  • PDF