• Journal of Life Science
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• v.34 no.4
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• pp.271-278
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• 2024

Redox factor (Ref)-1, a ubiquitously expressed protein, acts as a modulator of redox-sensitive tran- scription factors and as an endonuclease in the repair pathway of damaged DNA. However, the function of Ref-1 in the differentiation of monocytes into macrophages has not been defined. In this study, we investigated the effects of Ref-1 on the monocyte differentiation process using the human monocytic cell line THP-1. The differentiation agent PMA increased cell adhesion over time and showed a sig- nificant increase in phagocytic function but decreased the intracellular amount of Ref-1. Ref-1 inhibitor E3330 and Ref-1 knockdown using the siRNA technique reduced cell adhesion and the expression of differentiation markers, such as CD14, ICAM-1, and CD11b, by PMA stimulation. This means that the role of Ref-1 is absolutely necessary in the initial process of differentiating THP-1 cells stimulated by PMA. Next, the distribution of Ref-1 was examined in the cytoplasm and nucleus of THP-1 cells stimulated with PMA. Surprisingly, PMA stimulation resulted in the rapid translocation of Ref-1 to the nucleus. To prove that movement of Ref-1 to the nucleus is required for monocyte differentiation, a Ref-1 vector with the nuclear localization sequence (NLS) deleted was used. As a result, overexpression of ∆NLS Ref-1, which restricted movement to the nucleus, suppressed the expression of differentiation markers and notably reduced phagocytic function in PMA-stimulated THP-1 cells. In conclusion, these data suggest that the differentiation of monocytic THP-1 cells requires Ref-1 nuclear translocation during the initial process of biochemical events following stimulation from PMA.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.905-909
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• 2007

The dual-function protein redox factor-1 (Ref-1) is essential for base excision repair of oxidatively damaged DNA and also governs the activation of many redox-sensitive transcription factors. We examined the role of Ref-1 in regulation of nitric oxide (NO) synthesis employing adenoviral-mediatedoverexpression of Ref-1 in bradykinin-stimulated endothelial cells. Intracellular NO was detected with the NO-sensitive fluorophore DAF-2. Overexpression of Ref-1 potentiates bradykinin-stimulated NO production in endothelial cells. And, cells ifected with AdRef-1 showed higher fluorescence intensity compared with uninfected or AdD1312-infected cells. In parallel with this, over expression of Ref-1 also stimulated endothelial NO synthase (eNOS) enzyme activity, compared with unifected or AdD1312-infected cells, in bradykinin-stimulated cells as well as in unstimulated cells. These results suggest that Ref-1 implicates in endothelium-dependent vasorelaxation resulting from NO production in vascular system.

• Journal of Life Science
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• v.18 no.12
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• pp.1651-1656
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• 2008

Redox factor-1 (Ref-1) is essential for repair of oxidatively damaged DNA and also govern the reductive activation of many transcription factors. In this study, we examined the effect of overexpressed Ref-1 on AKT activation for nitric oxide (NO) production in mouse aortic endothelial (MAE) cells. Adenoviral-mediated overexpression of Ref-1 enhanced NO production in unstimulated- as well as bradykinin-stimulated MAE cells. Importantly, forced overexpression of Ref-1 induced direct phosphorylation of AKT in cells. And, a PI3K inhibitor wortmannin completely abolished the increase in AKT phosphorylation by stimulation of bradykinin and/or overexpressed Ref-1. In addition, inhibition of AKT activity with HA-tagged activation-deficient AKT suppressed Ref-1-induced endothelial NO synthase (eNOS) phosphorylation and resulted in a corresponding inhibition of unstimulated- and bradykinin-stimulated NO production. These results suggest that Ref-1 stimulates direct phosphorylation of AKT for eNOS enzyme activity in murine endothelial cells.

• Journal of Information Management
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• v.39 no.1
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• pp.221-237
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• 2008

CrossRef provides a various queries(OpenAPIs) which can be used for DOI & meta data lookup. CrossRef encourages publishers and library societies to develop diverse system by using the queries. In this thesis, CrossRef's queries are analyzed and DOI Lookup API which could automatically lookup the DOI by various methods was developed. I proposed that how institutions having their own meta data can use the developed API.

• Journal of Life Science
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• v.14 no.1
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• pp.26-31
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• 2004

Redox factor-1 (Ref-1), known as a redox regulator, controls the DNA binding of AP-1 and is activated in HT29 colon cancer cells by hypoxia in vitro. REF-1 also increases tile DNA binding affinity of Hypoxia-inducible Factor-lalpha$ (HIF-lalpha$), HIF-like Factor (HLF) and early growth response-1 (Egr-1) which induce expression of the genes involved in angiogenesis, so that we speculate that REF-1 may play a role in hypoxia-induced angiogenesis. In this research we tried to detect novel proteins interacting with REF-1 using Yeast two-hybrid system using full-length REF-1 cDNA as bait. As result of such screening we detected 3 positive clones. DNA sequencing and GeneBank search revealed that one of the clones contained the same sequences as M.musculus cDNA for tioredoxin.

• Journal of Information Management
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• v.27 no.1
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• pp.1-39
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• 1996

The purpose of this paper is to design a model of integrated OPAC called as EconRef. This model not only provides users of libraries with systematic, rapid information service, but also supports librarians to do their tasks effectively. The designed model is constructed based on two operating systems such as REGIS system and The Book House and is developed by using KPWin++ is an expert system shell which combines hypertext and expert system functions. The designed system consists of six modules ; three reference expert systems for document sources, experts and statistical sources; OPAC ; external database ; user's guide. For the evaluation of the designed system, performance of EconRef system is compared with that of the naive and expert reference librarians. And also the features of the system are compared with those of REGIS systems. The tests comparing BconRef system searching with librarians searching have shown that EconRef system is at least as good as searching with expert librarians and much superior to searching with naive librarians.

• The Journal of the Acoustical Society of Korea
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• v.14 no.4
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• pp.5-11
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• 1995

In this paper, we design the new high-speed seek algorithm which can access the data of disk (in 3.5-inch optical disk drive). When seek the target track on optical disk in high-speed using the ref-T. E(Reference Tracking Error) and sample pulse signal, it's always possible high-speed seek without re-check and compensation for error by the ref_T. E and sample pulse signal. The performance test result is represented as follow ; it is satify the average seek time of 28mses.

• Proceedings of the Korea Contents Association Conference
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• 2008.05a
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• pp.879-882
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• 2008

Abroad service of domestic academic thesis is very important for the enhancement of cited counting and Korea journals acknowledgement. The basis is to register DOI, the international academic thesis identifier at each thesis. It requires uneasy process depositing academic thesis to CrossRef which manages and registers DOI. KISTI has been conducting DOI/CrossRef deposit business object to domestic societies in scientific and technical field. In this thesis, we designed and implemented academic thesis DOI enrollment supporting system which is able to manage the required process on the online for the DOI/crossRef deposit business.

• Journal of Animal Science and Technology
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• v.63 no.2
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• pp.394-404
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• 2021

The objective of this study was to evaluate quality characteristics of low-nitrite emulsified-sausages (ESs, < 75 ppm) containing paprika oleoresin solution (POS) for replacing sodium nitrite (NaNO2). Pork ESs were prepared with four treatments (reference (REF), 150 ppm NaNO2; TRT1, 0 ppm NaNO2 + 0.1% POS; TRT2, 37.5 ppm NaNO2 + 0.1% POS; and TRT3, 75 ppm NaNO2 + 0.1% POS). The physicochemical and texture properties, microbial counts, residual nitrite and thiobarbituric acid reactant substances (TBARS) were measured during refrigerated storage of 35 days. Although TRT2 and TRT3 had lower levels of NaNO2, they had higher redness and yellowness than REF (p < 0.05). Microbial counts of total bacterial counts and Enterobacteriaceae of TRT2 and TRT3 were similar to those of REF (p > 0.05). Expressible moisture percentages (EM, %) of TRT2 and TRT3 were lower than those of REF (p < 0.05). TBARS values of TRT2 and TRT3 were not different from those of REF (p > 0.05). Among treatments, TRT1 had the highest TBARS values (p < 0.05). In conclusion, 0.1% POS in combination with 37.5 ppm NaNO2 would have quality characteristics similar to those of REF. Therefore, approximately 3/4 of the initial nitrite level could be replaced with 0.1% POS, and eventually developed healthier pork products.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.2B
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• pp.219-231
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• 2010

The Reference Evapotranspiration Calculator Software (REF-ET) supports computational guidelines for the reference evapotranspiration using seventeen FAO Penman-Monteith (PM) equations simultaneously such as the ASCE and FAO standardized forms. The REF-ET can conveniently consider missing data predictions and regional site characterizations, when reference ET is computed on monthly, daily, and hourly time steps. The applicability of the REF-ET was estimated to simulate the reference ET using hourly weather data from Seoul weather station for 29 years. The result found that the FAO24-Rd and 1957-Makk equations closely concerned with solar radiation parameter which were the most highly correlated to reference ET computed by pan coefficient. In addition, the 1957-Makk equation was identified as the most correct computational method for reference ET by analysis of bias and root mean square error. The 1957-Makk equation could predict the reference ET within the error of less than 1.06 mm/day, though all the other equations tended toward overestimation of predicting the reference ET in comparison with refecence ET of pan. The results of this study suggest that the REF-ET will be applicable to support reference ET estimation for a variety of field condition and time-scale.