• Journal of Animal Science and Technology
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• v.53 no.1
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• pp.43-50
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• 2011

An investigation was done to evaluate the effects of adding green tea probiotics, Alisma canaliculatum probiotics and fermented Kelp meal on the growth performance and immune response in sows and piglets. A total of 32 pregnant sows were assigned to 4 treatments in 8 replications with 1 sow as a replicate in this experiment from 2 months before parturition to 28 days after parturition. Three hundred nineteen (319) piglets were produced from experimental sows after parturition. The dietary treatments were CON group (without antibiotics), GTP (basal+green tea probiotics, 0.5% of the diet), ACP group (basal + Alisma canaliculatum probiotics, 0.5% of the diet) and FKM group (basal+fermented Kelp meal, 0.5% of the diet). Litter size and body weight at birth and weaning size and body weight at weanling in piglets were greater in additives group compared to CON group but no statistical differences were found (P>0.05). Diarrhea score showed a decreasing tendency with three additives groups compared to CON group. The lymphocytes in blood of sows fed ACP and FKM group were significantly higher compared to CON group (P<0.05), and the MID of GTP and FKM group were significantly lower compared to CON group (P<0.05). The serum IgG concentration in sows tended to increase with additives groups compared to CON group and the serum IgA and IgM concentration in GTP and FKM group were significantly higher compared to ACP group (P<0.05). The cortisol levels showed a decreasing tendency in additives groups without statistical differences among the groups (P>0.05). Based on these observations, it can be inferred that the GTP and FKM may have such efficacy as antibiotics in growing pigs.

• Korean Journal of Food Science and Technology
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• v.38 no.1
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• pp.104-108
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• 2006

Water extracts of Jehotang were evaluated for their growth-promoting effects on Bifidobacterium longum, Lactobacillus sp., L. acidophilus, and Clostridium perfringens. Addition of Jehotang water extract to modified EG media at 0.1 mg/mL increased growths of B. longum, Lactobacillus sp., and L. acidophilus, with 1.8-fold increase in growth of L. acidophilus compared to that of control. Studies on these strains by agar diffusion method showed Lactobacillus sp. and L. acidophilus were activated by addition of Jehotang extract at 10 mg/disc. Proliferation responses of mice splenocytes and Peyer's patch cells to ConA by LPS-stimulation at 500 mg/kg B.W./day Jehotang extract were investigated in vitro. Upon treatment of 1 mg/mL Jehotang water extract to mice, proliferations of splenocytes and Peyer's patch cells increased 1.4- and 1.6-fold compared to control, respectively. In mice administered Jehotang extract, production of intestinal secretory IgA (sIgA) increased 2.4-fold compared to control. These results indicate water extract of Jehotang stimulated intestinal immune system of mice. In mice treated with Jehotang extract, production of lymphocytes was 4% lower, whereas those of granulocytes and platelets were 4% and slightly higher than control, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.10
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• pp.1517-1522
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• 2002

The effect of post milking teat dipping on somatic cell count (SCC) of milk was determined in 20 Crossbred cows and 20 Murrah buffaloes selected from institute's herd. The animals were divided into two groups of 10 each. Animals of Group I (control) were teat washed with water before the milking while Group II animals were applied teat dipping solution after the completion of milking. The cows were milked 3 times a day while buffaloes were milked twice a day. The milk samples were collected from control and treated animals on day 0, 5, 10, 15, respectively. The milk samples were analyzed for milk constituents like fat, protein, lactose, chloride, IgG, NEFA, pH and EC and total and differential somatic cell counts. The changes in milk composition and somatic cell counts were significantly different (p<0.01) between the animals and between the breeds. However SCC, chloride content (p<0.05) and epithelial cells (p<0.01) varied during different days of study. The alterations in SCC, epithelial cells, TLC, lymphocyte, neutrophil, IgG, and protein content were significantly different (p<0.01) between control and treated groups. The pH, EC, protein, SCC, epithelial cells, lymphocyte and neutrophil cells of milk declined significantly (p<0.05) after the application of teat dipping, the respective values were 6.5 vs 6.40, 2.28 vs 2.37 mhos, 3.33 vs 4.04%, 1.00 vs $0.87{\times}10^5cells/ml$, 0.39 vs 0$0.34{\times}10^5cells/ml$, 0.36 vs $0.31{\times}1,000cells/ml$ and 0.17 vs $0.14{\times}1,000cells/ml$ in cows. However in buffaloes, epithelial cells, lymphocytes, neutrophils, EC and SCC declined (p<0.05) after application of teat dipping, the values being 0.37 vs $0.29{\times}10^5cells/ml$, 0.37 vs $0.25{\times}1,000cells/ml$, 0.14 vs $0.11{\times}1,000cells/ml$, 2.56 vs 2.37 mhos and 0.94 vs $0.73{\times}10^5cells/ml$, respectively. The study indicated that post milking teat dipping could be used as an effective method for the lowering of SCC in milk of crossbred cows and buffaloes.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.388-395
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• 2009

The current study was conducted to investigate the effect of early feed restriction (FR, 8 to 14 d of age) on growth performance and immune function in broiler chickens. Birds were fed corresponding diets from 3 to 35 d of age, which consisted of three phases: starter (3 to 7 d of age), FR (8 to 14 d of age) and re-alimentation (15 to 35 d of age) phases. During the FR period, each group of birds was fed the basal diet ad libitum (CON), 85% (EN85) and 70% (EN70) of lower calorie diet ad libitum (qualitative FR), and 85% (FI85) and 70% (FI70) of voluntary intake on a daily basis (quantitative FR). As a result, there was no statistical difference in weight gain, feed intake and feed conversion ratio between the CON and quantitative or qualitative FR groups during the entire (3 to 35 d) periods. In particular, the EN85 group resulted in a significant (p<0.05) increase in weight gain compared with the EN70, FI85 and FI70 groups. Plasma total protein and albumin at 14 d of age (during FR) were significantly (p<0.05) lower in the quantitative and qualitative FR groups, but these parameters at 35 d of age (after re-alimentation) were higher (p<0.05) in the EN85 and EN70 groups than in the CON group. Plasma IgG level was unaffected by dietary FR procedure. In cytokines, there was no significant difference in the expression of lymphocytic IL-4 and IFN-${\gamma}$ at 14 d of age between the FR and the CON groups, whereas lymphocytic IL-6 and iNOS expression were significantly (p<0.05) lower in FI85 and FI70 groups. Moreover, lymphocytic iNOS was also significantly (p<0.05) lower in birds fed qualitative and quantitative diets compared with those fed ad libitum. In the thymus, IL-4 expression was higher (p<0.05) in FI85 and FI70 groups, whereas IL-6 expression was lower (p<0.05) in the FI85 and FI70 groups than in the CON group. Thymic iNOS was significantly (p<0.05) lower in birds fed qualitatively and quantitatively restricted diets compared with those fed ad libitum. At 35 d of age, there was no difference in the expression of IL-4, IL-6 and IFN-${\gamma}$ of lymphocytes and thymus between the FR and CON groups. In conclusion, 85% of quantitative and qualitative FR would have a beneficial effect on the expression of some cytokines including IL-4 and iNOS without change in growth performance of birds.

• Clinical and Experimental Pediatrics
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• v.59 no.sup1
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• pp.49-52
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• 2016

X-linked agammaglobulinemia (XLA) is a hereditary humoral immunodeficiency that results from Bruton's tyrosine kinase (BTK ) gene mutations. These mutations cause defects in B-cell development, resulting in the virtual absence of these lymphocytes from the peripheral circulation. Consequently, this absence leads to a profound deficiency of lg all isotypes, and an increased susceptibility to encapsulated bacterial infections. A 15-month-old Korean boy presented with recurrent sinusitis and otitis media after 6 months of age, and had a family history of 2 maternal uncles with XLA. Laboratory tests revealed a profound deficiency of Ig isotypes, and a decreased count of $CD19^+$ B cells in the peripheral circulation. Based on his family history and our laboratory test results, he was diagnosed with XLA. We performed BTK gene analysis of peripheral blood samples obtained from family members to confirm the diagnosis. Mutational analysis revealed a novel hemizygous frameshift mutation (c.82delC, p.Arg28Alafs*5), in the BTK gene. His mother and maternal grandmother were heterozygous carriers of this mutation and his two maternal uncles were hemizygous at the same position. After XLA diagnosis, intravenous immunoglobulin (400 mg/kg, monthly) treatment was initiated; recurrent sinusitis and otitis media were subsequently brought under control. To our knowledge, this is the first reported case of a Korean pedigree with a novel mutation in the BTK gene.

• Molecules and Cells
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• v.40 no.9
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• pp.655-666
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• 2017

We constructed a large $na{\ddot{i}}ve$ human Fab library ($3{\times}10^{10}$ colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and ${\kappa}$ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically relevant antigens by phage display. We obtained a database of unique 7,373 VH and 41,804 VK sequences by 454 pyrosequencing, and analyzed the repertoires. The distribution of VH and VK subfamilies and germline genes in our antibody repertoires slightly differed from those in earlier published natural antibody libraries. The frequency of somatic hypermutations (SHMs) in heavy-chain complementarity determining region (HCDR)1 and HCDR2 are higher compared with the natural IgM repertoire. Analysis of position-specific SHMs in CDRs indicates that asparagine, threonine, arginine, aspartate and phenylalanine are the most frequent non-germline residues on the antibody-antigen interface and are converted mostly from the germline residues, which are highly represented in germline SHM hotspots. The amino acid composition and length-dependent changes in amino acid frequencies of HCDR3 are similar to those in previous reports, except that frequencies of aspartate and phenylalanine are a little higher in our repertoire. Taken together, the results show that this antibody library shares common features of natural antibody repertoires and also has unique features. The antibody library will be useful in the generation of human antibodies against diverse antigens, and the information about the diversity of natural antibody repertoires will be valuable in the future design of synthetic human antibody libraries with high functional diversity.

• Korean Journal of Agricultural Science
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• v.44 no.3
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• pp.409-415
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• 2017

This study was conducted to investigate the effect of supplementation of fermented wheat bran (FWB) on growth and blood characteristics in weaned pig. A total of 36 weaned pigs ($Landrace{\times}Yorkshire{\times}Duroc$; BW, $7.78{\pm}0.04kg$) were randomly allocated to three dietary treatments with different FWB concentrations (0, 0.5, and 1.0%), and each treatment had 3 replicate pens with 4 pigs per pen. The FWB was obtained from a mixture of wheat bran and two microbes (Lactobacillus plantarum M10 and Saccaromyces cerevisiae) and was determined to contain $10.19{\pm}0.27log\;CFU/g$ of L. plantarum and $7.73{\pm}0.38log\;CFU/g$ of S. cerevisiae. Experimental diets were prepared by mixing 0 (control), 0.5, or 1.0% of the FWB to the basal diet, and fed to the weaned pigs for 7 weeks. During the experimental period, the pigs had access to the diet and water ad libitum. Feed intake increased significantly in the 1.0% FWB group compared to the control and 0.5% FWB groups (p < 0.05), whereas the other growth parameters were not different among the treatment groups. White blood cells and lymphocytes were significantly decreased in the FWB treatment groups compared to the control group, but other blood corpuscles were not different among the treatment groups (p < 0.05). The pigs fed 0.5% FWB showed greater serum IgG than the control and 1.0% FWB groups (p < 0.05). In conclusion, the FWB fed to weaned pigs did not negatively affect their growth performance, but rather reduced mortality by fortifying immunity.

• Korean Journal of Veterinary Research
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• v.46 no.3
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• pp.225-234
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• 2006

In the present study toxicity and immunostimulating activity of the lectin(KML-C), which was extracted from Korean mistletoe(Viscum album coloratum) were investigated in swine. To determine the toxicity, lectin was injected into thigh or cervical muscles of 4-week-old piglets(Landrace) and observed clinically and pathologically. For determination of the immnunostimulating activity, lectin($0.7{\mu}g/kg$ of body weight)-adjuvanted vaccine of Aujeszky's disease virus(ADV)(NYJ1-87) which was inactivated by 0.2% formalin was injected into the cervical muscle of antibody-negative piglets in the same age group. Subpopulation of the immune cells and serum neutralizing(SN) antibodies in the piglets were examined after vaccination, and resistance of the piglets against challenge by virulent NYJ1-87 was further examined. The results were also compared with those from piglets injected with aluminum hydroxide [$Al(OH)_3$]-adjuvanted vaccine of inactivated NYJ1-87 and NYJ1-87 vaccine without adjuvant, and the results are as follows. By injection of lectin with $30{\mu}g/kg$ of body weight to the thigh muscle, all of 12 piglets died after signs such as dyspnea, fever, systemic erythema and subcutaneous hemorrhages, and lesions pertaining to poisonous hepatitis and dysfunction of kidney were observed. By injection of lectin with $7{\mu}g/kg$ of body weight to the thigh muscle, all of 12 piglets showed signs such as edema and cutaneous hemorrhage in the injected area, lameness and depression, and lesions pertaining to poisonous hepatitis and dysfunction of kidney were observed. By injection of lectin with 1, 3 and $5{\mu}g/kg$ of body weight to the thigh muscle of each one piglet, signs such as congestion, induration and grayish coloration in the injected area, depression and inappetence were observed in all piglets. Toxic changes were also observed in the liver and kidney of piglets by lectin of 3 and $5{\mu}g$. By injection of lectin with 0.5 and $0.7{\mu}g/kg$ of body weight to the cervical muscle of each 9 piglets, all piglets were clinically normal and there were no significant changes in blood counts and chemistry values. Whereas, epithelial swelling and vacuolation of convoluted tubules were observed from one piglet injected with lectin of $0.7{\mu}g$, and necrosis and fibrosis of muscular fiber were observed in the muscle of one piglet injected with lectin of $0.5{\mu}g$. Only population of sIgM+ B lymphocytes increased among immune cells in all of 15 piglets immunized with lectin($0.7{\mu}g/kg$ of body weight)-adjuvanted vaccine, while compared to those in $Al(OH)_3$-adjuvanted vaccine and vaccine without adjuvant. No additional stimulation to the immune cells was recognized when lectin was added to $Al(OH)_3$-adjuvanted vaccine. In piglets immunized with lectin-adjuvanted vaccine, SN titers in reciprocal values for loge were 1.3-4.0 at 1-4 weeks after vaccination, which was similar to those with 1.0-3.3 by vaccine without adjuvant but lower than those with 2.0-5.7 by $Al(OH)_3$-adjuvanted vaccine. Also, no additional increase in the SN titers was recognized when lectin was added to $Al(OH)_3$-adjuvanted vaccine. Piglets immunized with lectin-adjuvanted vaccine were resistant to challenge by the virulent NYJ1-87 at 4 weeks after vaccination, and the SN titers reached to 5.0 one week after challenge, which was higher than those with 4.0 by vaccine without adjuvant but somewhat lower than those with 7.7 by $Al(OH)_3$-adjuvanted vaccine.