• Pediatric Infection and Vaccine
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• v.14 no.1
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• pp.69-74
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• 2007

Purpose : Human coronanviruses (hCovs) including hCoV-229E and hCoV-OC43 have been known as etiologic agents of the common colds and were regarded as clinically insignificant agents. However, recent identification of hCoV-NL63 and hCoV-HKU1 in children with lower respiratory tract infections has evoked the clinical concerns about their prevalence and the clinical significance of these hCoVs in children. This study was performed to investigate the prevalence of hCoVs in children with community-acquired pneumonia. Methods : From March 2006 to January 2007, nasopharyngeal specimens collected from children hospitalized with pneumonia, were tested for the presence of common respiratory viruses (respiratory syncytial virus, influenza A, influenza B, parainfluenza viruses, and adenovirus) using multiplex reverse transcriptase polymerase chain reaction (RT-PCR). Human metapneumovirus (hMPV) infection was excluded by nested RT-PCR using primers for the F-gene. To detect the different strains of hCoVs, nested RT-PCR assays specific for hCoVNL63, hCoV-OC43, hCoV-229E, and hCoV-HKU1 were performed. Results : Out of the 217 nasopharyngeal aspirate from children aged under 15 years, respiratory syncytial virus (RSV) was detected in 32 patients, hMPV in 18, human parainfluenza virus in 10, influenza virus A in 2, and adenovirus in 6. HCoVs were detected by RT-PCR in 8 (3.7%) of the 217 patients, hCoV-229E in 1, hCoV-NL63 in 3, and hCoVOC43 in 4 patients. HCoV-HKU1 was not detected in this study population. Conclusion : Recently identified hCoV-NL63 and hCoV-HKU1 seemed to have a little clinical significance in Korean children with severe or hospitalized community-acquired pneumonia.

• BMB Reports
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• v.29 no.3
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• pp.210-214
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• 1996

The pH dependence of the kinetic parameters of mutant O-acetylserine sulfhydrylase (OASS) from Salmonella typhimurium LT-2 has been determined in order to obtain information on the chemical mechanism. The initial velocity pattern obtained by varying the concentrations of OAS at several fixed concentrations of TNB, shows an intersection on the left of the ordinate at pH 7.0, indicating that the kinetic mechanism is a sequential mechanism in which substrate inhibition by OAS is observed while the wild type enzyme showed a ping pong mechanism. The values of $V/E_t$, $V/K_{OAS}E_{t}$ and $V/K_{TNB}E_{t}$ decreased by about 68%, 14% and 16% as compared with the wild type enzyme. The $V/K_{OAS}E_{t}$ is a pK of 6.5 on the acid side of the pH profile, and the $V/K_{TNB}$ is pH independent. As compared with the wild type enzyme, the pKs in the V/K profiles are shifted, reflecting that binding of the cofactor in free E:OAS is less asymmetric.

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.183-187
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• 2015

We use ALLWISE data release W1- and W2-band epoch photometry collected by the Wide-Field Infrared Survey Explorer (WISE) to determine slopes of the period-luminosity relations for RR Lyrae stars in 15 globular clusters in the corresponding bands. We further combine these results with V- and K-band photometry of Galactic field RR Lyrae stars to determine the metallicity slopes of the log $P_F-[Fe/H]-M_K$, log $P_F-[Fe/H]-M_{W1}$, and log $P_F-[Fe/H]-M_{W2}$ period-metallicity-luminosity relations. We infer the zero points of these relations and determine the kinematical parameters of thick-disk and halo RR Lyraes via statistical parallax, and estimate the RR Lyrae-based distances to 18 Local-Group galaxies including the center of the Milky Way.

• Microbiology and Biotechnology Letters
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• v.44 no.3
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• pp.333-340
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• 2016

An alkaline protease was purified and characterized from an alkalophilic microorganism, Bacillus sp. DK1122, isolated from soil in central Korea. The optimum temperature and pH for the growth of the producer strain were 40℃ and pH 9.0, respectively. The protease was produced aerobically at 40℃ after 24 h incubation in modified Horikoshi I medium (pH 9.0) containing 0.5% (w/v) glucose, 0.8% (w/v) yeast extract, 0.5% (w/v) polypeptone, 0.1% (w/v) K₂HPO₄, 0.02% (w/v) MgSO₄·7H₂O, 1% (w/v) Na₂CO₃, and 3% (w/v) NaCl. The alkaline protease was purified by 70% ammonium sulfate precipitation of the culture supernatant of Bacillus sp. DK1122, followed by CM-Sepharose chromatography. The molecular weight of the enzyme was estimated to be 27 kDa on the basis of SDS-PAGE. The optimum temperature and pH for the protease activity were 60℃ and pH 9.0, respectively. Addition of CaCl₂ increased the thermal stability of the purified protease, where 90% of protease activity was retained at 60℃ for up to 3 h. Consequently, it is expected that the alkaline protease from this study, exhibiting stability at pH 7–9 and 60℃, may be promising for application in the food and detergent industries.

• Journal of the Chungcheong Mathematical Society
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• v.14 no.2
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• pp.9-17
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• 2001

Vogiouklis introduced $H_v$-hyperstructures and gave the "open problem: for $H_v$-groups, we have ${\beta}^*={\beta}^{\prime\prime}$. We have an affirmative result about this open problem for some special cases. We study ${\beta}$ relations on $H_v$-quasigroups. When a set H has at least three elements and (H, ${\cdot}$) is an $H_v$-quasigroup with a weak scalar e, if there are elements $x,y{\in}H$ such that xy = H \ {e}, then we have (xy)(xy) = H.

• Proceedings of the IEEK Conference
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• 2001.06b
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• pp.229-232
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• 2001

4H-SIC(silicon carbide) MESFET large signal model was studied using modified Materka-Kacprzak large signal MESFET model. 4H-SiC MESFET device simulation have been conducted by Silvaco's 2D device simulator, ATLAS. The result is modeled using modified Materka large signal model. simulation and modeling results are -8V pinch off voltage, under $V_{GS=0V}$, $V_{DS=25V}$ conditions, $I_{DSS=270㎃}$mm, $G_{m=45㎳}$mm were obtained. Through the power simulation 2GHz, at the bias of $V_{GS=-4V}$ and $V_{DS=25V}$, 10dB Gain, 34dBm(1dB compression point)output power, 7.6W/mm power density, 37% PAE(power added efficiency) were obtained.d.d.d.

• Proceedings of the KIEE Conference
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• 2005.07c
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• pp.2149-2152
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• 2005

가속기 연구소에서 500kV 고전압 다중채널 다중갭(MGMC) 스위치를 개발하였다. 이 다중 채널 다중 갭 스위치는 개스를 채우는 방식으로 자발 방전전압을 증가 시킬 수 있는 10단의 갭으로 구성이 되어 있다. 멀티갭 스위치의 구조는 스위치의 자체 인덕턴스를 증가시키는 결과를 가져온다. 따라서 이러한 문제점을 해결하기 위하여 다중채널을 사용하여 인덕턴스를 줄이는 스위치로 구조를 설계하였다. 제작된 스위치는 500kV의 Marx generator를 사용하여 시험을 하였다. 본 논문에서는 개발된 스위치 선계 및 스위치의 특성에 대하여 알아보고자 한다.

• Proceedings of the KIPE Conference
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• 2016.07a
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• pp.279-280
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• 2016

전기자동차를 위한 양방향 탑재형 충전기의 장내구성을 달성하기 위해 전해캐패시터를 사용하지 않는 전력변환장치가 요구된다. 본 논문에서는 V2G-V2H 기능을 갖는 무 전해캐패시터 양방향 탑재형 충전기의 제어알고리즘을 제안한다. 제안하는 양방향 충전기는 정현파 충전방식이 적용되었고 인버터만 제어하여 V2G-G2V-V2H 모드전환이 끊김없는 장점을 갖는다 제안하는 알고리즘을 검증하기 위한 3.3kW급 시작품의 시험결과로 타당성을 검증하였다.

• Journal of Microbiology and Biotechnology
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• v.18 no.6
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• pp.1186-1190
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• 2008

Single-chain Fv (scFv) antibody against c-Met is expected to be employed in clinical treatment or imaging of cancer cells owing to the important biological roles of c-Met in the proliferation of malignancies. Here, we show that the productivity of scFv against c-Met in Escherichia coli is significantly influenced by the orientation of its variable domains. We generated anti-c-Met scFv antibodies with two different domain orders (i.e., $V_L$-linker-$V_H$ and $V_H$-linker-$V_L$), expressed them in the cytoplasm of E. coli trx/gor deleted mutant, and compared their specific activities as well as their productivities. Productivity of total and functional anti-c-Met scFv with $V_H/V_L$ orientation was more than five times higher than that with $V_L/V_H$ format. Coexpression of DsbC enhanced the yield of soluble amounts of anti-c-Met scFv protein for both constructs. The purified scFv antibodies of the two different formats exhibited almost the same antigen-binding activities. We also compared the productivities and specific activities of anti-c-Met diabodies with $V_H/V_L$ or $V_L/V_H$ formats and obtained similar results to the case of scFv antibodies.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.799-802
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• 2002

Continuously variable transmission(CVT) mechanisms considered here combine the functions of a K-H-V type differential gear unit and a V-belt type continuously variable unit(CVU). As combining the functions of a K-H-V type differential gear unit and a V-belt type CVU, 24 different mechanisms are presented. Some useful theoretical formula related to speed ratio, power flow and efficiency are derived and analyzed. These mechanisms have many advantages which are the decrease of CVT size, the increase of overall efficiency, the extension of speed ratio range, and the generation of geared neutral.